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Development of Self-Managed Food Sanitation Check-List and On-Site Monitoring of Food Sanitation Management Practices in Restaurants for Control of Foodborne Illness Risk Factors (식중독 발생 위험요인 관리를 위한 외식업체 자가위생관리점검표 개발 및 현장모니터링)

  • Chung, Min-Jae;Choi, Jung-Hwa;Ryu, Kyung;Kwak, Tong-Kyung
    • Korean journal of food and cookery science
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    • v.26 no.5
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    • pp.603-616
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    • 2010
  • Based on recent dramatic increases in foodborne outbreaks in restaurants, self-managed sanitation systems are now recommended to control contributing risk factors. This study aimed to improve sanitation management practices in restaurants and had two objectives. First, we tried to develop a self-managed sanitation check-list, including risk factors contributing to foodborne illness and Korean food hygiene regulation articles. We also tried to evaluate current sanitation management practices in restaurants according to operation and restaurant type. Thirty restaurants were evaluated by on-the-spot inspectors using an auditing tool consisting of four dimensions, seventeen categories, and forty-one items. Total compliance rate categorized by operation type significantly differed between chain restaurants and self-managed restaurants, with values of 85.5% and 51.6%, respectively. Therefore, self-managed restaurants, which showed the lowest compliance rate of below 30.0%, need more strict control to improve current unsanitary management practices, specifically relating to 'sterilization of knives, chopping boards, and wiping cloths', 'sanitation training', 'not allowing access into the kitchen to outsiders', 'handling of food or utensils on shelves at a 15 cm distance away from floor', 'prevention of cross-contamination of cooked foods or vegetables', and 'records of kitchen access or inspection'. Thus, an effective food sanitation system is essential and should be implemented to improve the existing sanitary conditions in restaurants. However, the most important factor to achieving food sanitation management objectives is food handlers' self-motivation.

Quantifying Uncertainty of Vitamin C Determination in Infant Formula by Indophenol Titration Method (인도페놀 적정법에 의한 성장기용조제식 중 비타민 C 함량분석의 측정불확도 산정)

  • Jun, Jang-Young;Kwak, Byung-Man;Ahn, Jang-Hyuk;Kong, Un-Young
    • Korean Journal of Food Science and Technology
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    • v.37 no.3
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    • pp.352-359
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    • 2005
  • Uncertainty involved during determination of vitamin C content in infant formula was quantified by indophenol titration method. Uncertainty sources in measurand, such as purity, weight, final volume of standard, volume of standard solution used for titration, sample weight, final volume of sample, extraction solution used for titration, titration of extraction solution and standard solution by indophenol solution were identified and used as parameters for combined standard uncertainty based on Guide to the expression of uncertainty in measurement (GUM) and Draft EURACHEM/CITAC Guide. Uncertainty parameters of each source in measurand were identified as resolution, reproducibility and stability of chemical balance, standard material purity, repeatability, reproducibility, end point of titration, 1 mL pipet, 5 mL autopipet, and 100 mL mass flask. Each uncertainty component was evaluated by types A and B and included to calculate combined uncertainty. Analytical test result for traceability under laboratorial conditions using Certified Reference Material (CRM) test was certified as $108.4{\pm}1.7mg/100g$, which was within CRM certification range of $114.6{\pm}6.6mg/100g$. Uncertainty test result of vitamin C content of 5 g sampling was $56.7{\pm}2.44mg/100g$. Uncertainty could be reduced by identification of uncertainty sources and components related with vitamin C determination by indophenol titration method and by decreasing uncertainty sources and components.

Studies on the Processing of Chestnut (Castanea pubinervis Schneid) Part 1. Trials on the Raw Material Adaptability for Processing and Colored Products Development (밤(Castanea pubinervis schneid) 가공(加工)에 관(關)한 연구(硏究) 제1보 밤의 가공(加工) 적성(適性) 및 유색(有色) 가공품(加工品) 개발(開發)을 중심(中心)으로)

  • Suh, K.S.;Han, P.J.;Lee, S.J.
    • Korean Journal of Food Science and Technology
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    • v.6 no.2
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    • pp.98-108
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    • 1974
  • Fourteen varieties of Korean chestnut were subjected to the test of adaptability for processing and the possibilities of colored products development. The fruit size of Korean chestnut ranged $11{\sim}18g$ and these small fruits could not be expected to be utilized for the processing of Marron glaces which requires larger size as $25{\sim}30g$. As the storge period is extended the discoloration tendency of chestnut flesh was increasingly developed, however, the fresh chestnut has shown layer-separating phenomenon and ragged surface of fruit which delivers disagreeable appearance to the finished product. The principal factors of discoloration occurred during processing were the behavior of tannin and darkening rate shown on flesh differed each other among varieties; the Chukpa and Yuma variety have exhibited the most serious discoloration and the Taab-b variety, the lightest. Taab-b variety in this connection could be expected to be available for Kanroni processing. For the industrialization of chestnut processing the flame-scorched peeling method is advisable. The capacity of this method is proportional to the square of scorching radius and highly flexible in controlling its performance. As for the processing of colored product, the sugar dehydration and coating and the sugar penetration method demand further study in basical views; however, the canned product of chestnut-redbean has shown the possibility of being utilized as a substitute for or paralleled use with the sugar-syruped canned product which so far has been considered as the only item of export to Japan.

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Effects of soil Moisture Levels on Growth and Dry Matter Accumulation of Sorghum and Corn II. Changes of dry matter accumulation and chemical composition (토양수분함량이 수수속작물과 옥수수의 생육 및 건물합성에 미치는 영향 II. 건물축적 및 성분함량의 변화)

  • 한흥전;한민수;안수봉
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.5 no.2
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    • pp.152-161
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    • 1985
  • To examine the effects of different levels of soil moisture on dry matter production and chemical compositions of sorghum cv. Pioneer 931, sorghum-sudangrass hybrid cv. Pioneer 988, sudangrass cv. Piper, and corn cv. Suweon 19. Soil moisture contents were maintained with approximately 100, 80 and 40% of field moisture capacity. The results are summarized as follows; 1. The highest dry matter yields per plant were found at 60% soil moisture level with 176.2g, 180.8g and 164.0g for pioneer 931, Pioneer 988 and corn, respectively. 2. Dry matter accumulation in accordance with soil moisture levels and growth stages of all crops except sudangrass were in the order of 60>40>80>100% soil moisture level. 3. The highest absolute growth rate (AGR) of sorghum, sorghum-sudangrass hybrid and corn was shown at 60% soil moisture level, that of sudangrass was shown at 80% soil moisture level. The relative growth rate (RGR) of all crops was high in the early growing stage and was low at maturity. The highest net assimilation rate (NAR) of all crops was found at 60% soil moisture level with $72-467g/m^2/day$ from June 29 to July 5. 4. The higher crude fiber content in leaf of Pioneer 931 was shown at 100% and 80% soil moisture levels with 28.6-28.8%, that of corn had no significant difference among soil moisture levels. The crude protein content in leaf of all crops was 14.2-21.6% at 60% soil moisture level, 13.8-16.0% at 40% soil moisture level, and 7.3-13.9% at above 80% soil moisture levels, respectively. 5. The crude fiber content in stern of all crops and all soil moisture levels was 24.6-36.7%, and the crude protein content in stem was 2.5-5.3% in dry matter basis.

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Growth and Yield Comparisons among Barley Varieties with Different Resistance to Barley Mosaic Virus (보리 모자이크 바이러스 저항성 정도가 상이한 보리 품종간 생육 및 수량 비교)

  • Park Jong-Chul;Lee Mi-Ja;Choi In-Bae;Kim Mi-Jung;Park Chul-Soo;Kim Jung-Gon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.5
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    • pp.477-482
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    • 2006
  • Viral diseases, especially Barley yellow mosaic virus (BaYMV) and Barley mud mosaic virus (BaMMV), have been most serious in barley fields. In this study, we investigated the effect of different level of resistance to viral diseases on the plant growth and yield in barley. Various viral disease symptoms on leaves of overwintered plants were similar between medial-resistant and susceptible varieties of Saessalbori and Baegdong. In diagnosis of virus infection, BaYMV and BaMMV were detected in Saessalbori and Baegdong, but not in the resistant variety, Naehanssalbori. Plant height was restrained about $11{\sim}12cm$ prior to heading in Saessalbori and Baegdong comparing to Naehanssalbori. Even if both varieties were medial resistant to virus diseases, Saessalbori was different from Baegdong in heading date and culm length due to its recovery from viral damages prior to heading. Both medial-resistant and susceptible varieties were quite different from the resistant variety in yield components such as heading date, number of spikes and culm length when evaluated in the virus-infected or non-infected field. Baegdong delayed 7 days in heading date and reduced by more than 50% in culm length and spike numbers as compared to Naehanssalbori. On the other hand, Saessalbori showed similar heading date, but was shorter by 20% in culm length than Naehanssalbori. Three varieties tested in the non-infected field over two years were not significantly different for yield potential with ranges of $340{\sim}405kg/10a$. However, significant yield reduction (P<0.01) was observed in Saessalbori and Baegdong with ranges of $108{\sim}288kg/10a$ as compared to Naehanssalbori (391 kg/10a) when tested in the virus-infected field. Yield potentials of Saessalbori and Baegdong reduced by 35 and 63%, respectively in the virus-infected field as compared to those in the non-infected field. Our results showed that damages from virus diseases were significant on the early plant growth to yield and its components in barley.

Topographical Change Monitoring of the Sandbar and Estimation of Suspended Solid Flux in the Nakdong River Estuary - Focused on Jinudo - (낙동강 하구역 사주지형 변동과 부유사(SS) 수송량 산정 - 진우도를 중심으로 -)

  • Lee, I.C.;Lim, S.P.;Yoon, H.S.;Kim, H.T.
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.11 no.2
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    • pp.70-77
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    • 2008
  • In this study, to establish countermeasure from marine casualties as a basic study fur long-term prediction of topographical change around Jinudo in the Nakdong river estuary, spatio-temporal topographical change monitoring was carried out. Also, in order to estimate the deposition variations concerning SS (Suspended Solid) flux which moved at St.S1 during neap and spring tide, respectively. From the topographical monitoring, it was found that the annual mean ground level and deposition rate were 141 mm and 0.36 mm/day and all parts except the northern part of Jinudo had the active topographical changes and a tendency to annually deposit. From vertical distribution of SS net fluxes, $SS_{LH}$ (latitudinal SS net flux) during spring tide overall flows average 28 $kg/m^2/hr$ (eastward), and $SS_{LV}$ (longitudinal SS net flux) flows average 11.1 $kg/m^2/hr$ (northward). And, $SS_{LH}$ overall flows average 4.8 $kg/m^2/hr$ (eastward), and $SS_{LV}$ flows average 1.5 $kg/m^2/hr$ (northward) during neap tide similar with spring tide. The depth averaged values of the latitudinal and longitudinal SS net fluxes during spring tide were approximately 6 times higher than those during neap tide. As result of, it was considered that topographical change of southern part of Jinudo was affected by resuspension of bottom sediments due to strong current in bottom layer during flood flow.

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Chemoprevention of Helicobacter pylori-associated Gastric Carcinogenesis in a Mouse Model; Is It Possible?

  • Hahm, Ki-Baik;Song, Young-Joon;Oh, Tae-Young;Lee, Jeong-Sang;Surh, Young-Joon;Kim, Young-Bae;Yoo, Byung-Moo;Kim, Jin-Hong;Ha, Sang-Uk;Nahm, Ki-Taik;Kim, Myung-Wook;Kim, Dae-Yong;Cho, Sung-Won
    • BMB Reports
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    • v.36 no.1
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    • pp.82-94
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    • 2003
  • Although debates still exist whether Helicobacter pylori infection is really class I carcinogen or not, H. pylori has been known to provoke precancerous lesions like gastric adenoma and chronic atrophic gastritis with intestinal metaplasia as well as gastric cancer. Chronic persistent, uncontrolled gastric inflammations are possible basis for ensuing gastric carcinogenesis and H. pylori infection increased COX-2 expressions, which might be the one of the mechanisms leading to gastric cancer. To know the implication of long-term treatment of antiinflammatory drugs, rebamipide or nimesulide, on H. pylori-associated gastric carcinogenesis, we infected C57BL/6 mice with H. pylori, especially after MNU administration to promote carcinogenesis and the effects of the long-term administration of rebamipide or nimesulide were evaluated. C57BL/6 mice were sacrificed 50 weeks after H. pylori infection. Colonization rates of H. pylori, degree of gastric inflammation and other pathological changes including atrophic gastritis and metaplasia, serum levels and mRNA transcripts of various mouse cytokines and chemokines, and NF-${\kappa}B$ binding activities, and finally the presence of gastric adenocarcinoma were compared between H. pylori infected group (HP), and H. pylori infected group administered with long-term rebamipide containing pellet diets (HPR) or nimesulide mixed pellets (HPN). Gastric mucosal expressions of ICAM-1, HCAM, MMP, and transcriptional regulations of NF-${\kappa}B$ binding were all significantly decreased in HPR group than in HP group. Multi-probe RNase protection assay showed the significantly decreased mRNA levels of apoptosis related genes and various cytokines genes like IFN-$\gamma$, RANTES, TNF-$\alpha$, TNFR p75, IL-$1{\beta}$ in HPR group. In the experiment designed to provoke gastric cancer through MNU treatment with H. pylori infection, the incidence of gastric carcinoma was not changed between HP and HPR group, but significantly decreased in HPN group, suggesting the chemoprevention of H. pylori-associated gastric carcinogenesis by COX-2 inhibition. Long-term administration of antiinflammatory drugs should be considered in the treatment of H. pylori since they showed the molecular and biologic advantages with possible chemopreventive effect against H. pylori-associated gastric carcinogenesis. If the final concrete proof showing the causal relationship between H. pylori infection and gastric carcinogenesis could be obtained, that will shed new light on chemoprevention of gastric cancer, that is, that gastric/cancer could be prevented through either the eradication of H. pylori or lessening the inflammation provoked by H. pylori infection in high risk group.

Sex Determination of Porcine IVF Embryos by Polymerase Chain Reaction (PCR) (중합효소연쇄반응을 이용한 돼지 체외수정란의 성감별)

  • 강미선;김용준;이해이;유일정
    • Journal of Embryo Transfer
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    • v.16 no.3
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    • pp.203-211
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    • 2001
  • This study was carried out to determine sex of porcine embryos produced by in vitro fertilization. Porcine oocyte-cumulus complexes were cultured in BSA-free North Carolina State University (NCSU) 23 medium containing porcine follicular fluid (10%), cystein (0.1 mg/ml) and hormonal supplement (10 IU eCG and 10 IU hCG per ml) for 20~22 hrs. They were then cultured in the same medium but without hormonal supplement for additional 20~22 hrs. After culture, cumulus cells were removed and oocytes were co-incubated for 6 hrs with four different concentrations (5$\times$10$^4$, 2.5$\times$ 10$^{5}$ , 5.0$\times$10$^{5}$ and l0$\times$10$^{5}$ ) of porcine sperm. After fertilization, oocytes were transferred into NCSU 23 with 0.4% BSA medium. The cleavage and blastocyst formation rates were evaluated at 48 and 144 hrs, respectively. In this study, the polymerase chain reaction (PCR) was used to determine the sex of porcine embryos in the stage of blastocyst. The PCR was performed using a set of oligonucleotide primers (5‘-TCATGGACCAGGTAGGGAAT-3', 5’-GAAAGACACGTCCTTGGA GA-3') for 491 bp fragment of porcine male-specific DNA sequence. In the flour different sperm concentration (5$\times$10$^4$, 2.5$\times$10$^{5}$ , 5.0$\times$10$^{5}$ and l0$\times$10$^{5}$ ) for fertilization condition, the cleavage rate was 55.95, 67.88, 60.18 and 47.60%, respectivety, and the development rate of blastocysts was 16.03, 20.40, 21.41 and 12.37%, respectively. At 5.0$\times$10$^4$and 2.5$\times$10$^{5}$ of sperm concentrations per ml cleavage rate and development rate of blastocyst were higher than those of 5.0$\times$10$^4$and l0$\times$10$^{5}$ of sperm concentration (P<0.01). The male of porcine embryos was detected at 491 bp by PCR, and 18 of the 31 porcine blastocysts were the male (58.1%) and the rest 13 were the female(41.9%).

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Fertility status of Jeju volcanic ash soil and its improvement (제주도전토양(濟州道田土壤)의 비옥도현황(肥沃度現況)과 개량(改良))

  • Ryu, In Soo;Yoo, Sun-Ho;Yoon, Jung Hai
    • Korean Journal of Soil Science and Fertilizer
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    • v.8 no.3
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    • pp.121-133
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    • 1975
  • Soils of the agricultural land derived mostly from volcanic ash in Jeju island may be characterized by relatively high PH, high contents of organic matter and exchangeable bases, and significantly low content of available phosphorus. The PH, organic matter, and available phosphorus of the soils in northern Jeju are 6.1, 12.6%, and 23 ppm, while those of the soils in the southern Jeju are 6.4, 3.7 %, and 76 ppm respectively. Upland soils in Jeju may be classified into 4 groups on the basis of soil fertility: black soil, very dark brown soil, dark brown, and red yellow soil. The organic matter content of black soil, very dark soil and dark brown soil is 15%, 7%, and 3% respectively. The soils of high organic matter content show the high phosphorus absorption coefficient, low content of available phosphorus, and low degree of the base saturation. The soil productivity for barley in the northern Jeju is higher than that in the southern Jeju and the productivity in the western Jeju is higher than that in the eastern part. The productivity for barley is in decreasing order of dark brown soil, very dark brown soil and black soil. Yields of potato and sweet potato in Jeju are higher than in the mainland. Those crops are considered to be tolerant to aluminumtoxicity. The response of sweet potato to nitrogen, phosphorus, and potassium in Jeju soils is lower than that in the mainland, while the response of barley and rape to nitrogen and phosphorus is higher than that in the mainland. The response to fertilizer applied is greater in the northern Jeju than in the southern Jeju. Lime requirement for the Jeju soils calculated on the basis of laboratory tests is so tremendous that single application of lime required might induce adverse effect. Most of the phosphorus applied to the Jeju soils is readily fixed as unavailable form and application of phosphorus in the level of 30 to 40 kg/10a is thought to give little effect.

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Effect of Soil Salinity for Ecological Restoration in the Reclaimed Area of Seasides (임해매립지의 생태계 복구를 위한 토양중 염류의 활성도 분석)

  • Chang, Kwan-Soon;Kim, Hyong-Bok
    • Korean Journal of Soil Science and Fertilizer
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    • v.32 no.2
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    • pp.147-154
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    • 1999
  • This study was carried out to obtain reasonable management method of salt-affected soil for ecological restoration in the reclaimed land. Chemical properties of reclaimed soil was investigated base on reclamation years. Ionic acitivity in soil and satruration extract were analyzed to estimate the effect of salt interception by planting ground treatment. The soil porperties of reclaimed land was saline-sodic soil with $11.3dSm^{-1}$ of electrical conductivity, 34.8% of exchangeable sodium percent in first reclamation year. Electrical conductivity, exchangeable sodium and exchangeable chlorine were remarkedly decreased during six years after reclamation but chemical properties of reclaimed soil was unsuitable status for tree growth. Exchangeable sodium perecnt was higher in the neighborhood parks and street tree sites than in the buffer green spaces and was higher in subsoil than in topsoil of profile in all sites. Content of soduim, chloride and sulfate in saturation extract were more than other ions. Content of soduim and chloride were higher in the neighborhood parks and street tree sites than in the buffer green spaces and were higher in subsoil than in topsoil. Content of calcium plus magnesium of soil was higher in the buffer green space than in the neighborhood park and street tree but content of calcium and magnesium in saturation extract were higher, as result from exchangeable sodium, in the neighborhood parks and street tree sites than in the buffer green spaces. Concentration of salt in soil showed the difference with mounding height and planting ground treatment. The lowest concentration of salt appeared in buffer green spaces and street tree sites was the highest. Salt interception by mounding height in the same planting ground treatment was more effective 120cm of mounding height than 70cm of mounding height.

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