• Animal Bioscience
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• 제35권4호
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• pp.567-576
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• 2022

Objective: An experiment was conducted to determine the effect of algae-based antioxidant containing Se yeast (EconomasE^®) on the growth performance, visceral organ weight, meat quality, and oxidative stability of broiler chickens. Methods: Nine hundred sixty, day-old male broiler chickens (Cobb, 43.97±0.55 g) were divided into three dietary treatments and allocated into 12 deep litter pens in a completely randomized design giving 4 replicate cages for each treatment. Three dietary treatments were: i) control (CON, basal diet with sufficient nutrient), ii) vitamin E (VitE, basal diet supplemented with 100 IU VitE), and iii) Algae-based antioxidant containing Se yeast (EcoE, basal diet supplemented with 0.2% algae-based antioxidant containing Se yeast: EconomasE^®). Maize soybean meal based basal diets were formulated to meet or exceed the nutrition requirement for broiler chickens. Chickens were fed ad-libitum experimental diets during the 42 days experiment period. On days 21 and 42, body weight and feed intake were measured to calculate the feed conversion ratio of the chickens. Intestine and visceral organs were measured together with meat quality and oxidative stability on days 14 and 42. Results: Chickens fed with EcoE showed improved (p<0.05) growth performance, meat quality, and higher (p<0.05) oxidative stability compared to the chicken fed on CON. Moreover, broiler chickens fed with EcoE showed similar (p<0.05) growth performance with better (p<0.05) meat quality and higher oxidative stability compared to the broiler chickens fed VitE (p<0.05). Conclusion: The algae-based antioxidant containing Se yeast can be supplemented into commercial broiler diets as a substitution of VitE while maintaining growth performance with enhancing meat quality and oxidative stability of the broiler chickens.

• 한국미생물·생명공학회지
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• 제15권6호
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• pp.396-401
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• 1987

$\beta$-Galactosidase 유전자 조작된 두 대장균 숙주-벡터 시스템의 단백질 생산가 배양특성을 비교하였다. Tac 프로모터를 갖는 Escherichia coli JM109/ pTBG10 균주가 pL 프로모터를 갖는 E. coli MH 3000(pRKcI857)/ pASl(lacz) 균주보다도 단백질 생산성에 있어서 훨씬 우수하였으며 초기 및 중간 대수 증식기에서의 발현 유도가 단백질 생산에 있어서 유리함을 관찰하였다. 또한 유전자 발현시기에 있어서는 산소요구량이 매우 높았으나 pH를 일정하게 조절함에 의해 산소요구량을 어느 정도 낮출 수 있었고 생산성도 향상됨을 관찰할 수 있었다. 고생산성 균주(E. coli JM109/pTBG10)의 경우 플라스미드 함유 균체의 플라스미드 상실 균체에 대한 비증식속도의 비 $\mu$ + /$\mu$- 가 저생산성 균주보다도 낮았으며 이것은 유전자발현에 따른 단백질 합성부담이 매우 큰 때문으로 판단되었다. 또 플라스미드 안정성을 30세대까지 추적하였는바 유전자 발현 조건하에서 두 균주 모두 25세대 후 10% 정도의 안정성을 보였으나 E. coli JM109/pTBG10 균주의 안정성이 상대적으로 우수하다고 판단되었다.

• 상하수도학회지
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• 제29권3호
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• pp.407-414
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• 2015

The effect of activated carbon particle diameter (i.e. US sieve No. $8{\times}10$ ($d_p{\approx}2.19mm$), $18{\times}20$ ($d_p{\approx}0.92mm$), $50{\times}60$ ($d_p{\approx}0.27mm$) and $170{\times}200$ ($d_p{\approx}0.081mm$) on caffeine adsorption is investigated. BET surface area was increased with decreasing particle diameter ($d_p$), and caffeine adsorption rates increased with decreasing $d_p$. Moreover, pseudo-second order model is predicted the experimental data more accurately than pseudo-first order model, and the fastest rate constant ($k_2$) was $1.7g\;mg^{-1}min^{-1}$ when $d_p$ was 0.081 mm. Surface diffusion coefficient (Ds) was decreased with decreasing $d_p$ based on the minimum sum of square error (SSE). Practically, certain ranges of Ds are acceptable with high reliability ($R^2$) and it is determined that the effect of $d_p$ on Ds is unclear. The effect of pH on caffeine adsorption indicated the dependency of m/L ratio (mass liquid ratio) and $pH_{pzc}$. The $pH_{pzc}$ (i.e. $7.9{\pm}0.2$) was not affected by $d_p$. The higher caffeine adsorption at pH 4 and pH 7 than at pH 10 is due to $pH_{pzc}$, not $pk_a$ of caffeine.

• 한국식품영양과학회지
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• 제31권2호
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• pp.184-188
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• 2002

시료 달걀의 난황 속에는 올레산과 팔미트산이 가장 많이 함유되어 있었고, 저장 1, 2주에 그 함량이 최고점으로 도달 하였으며, 3주 때는 감소하였다. 일반란의 경우 저장기간이 길어질수록 팔미트산과 올레산은 증가 후 다시 감소하였고, 리놀레산은 증가하였다. 인삼란의 경우 팔미트산과 스테아르산이 감소 경향을 보였다. DHA란의 팔미트산과 올레산의 변화는 없었으나, 리놀레산이 증가하였다 감골란은 올레산이 급격히 감소의 경향을 보이는 반면, 팔미트산은 증가 후 감소의 경향을 반복하였다. 저장기간이 길어짐에 따라 포화 지방산의 함량은 감소하고 불포화지방산의 함량은 증가하는 경향을 보였다. 구입 당시 모든 달걀에 나타나지 않았던 DHA가 저장기간 동안에 극미량 검출되었다. 한편, n-6/n-3 비율은 일반란이 저장 1~3주, 감골란은 구입 당시, DHA란은 저장 0~3주의 저장기간 동안 섭취 권장 범위에 해당되었다. 저장기간 동안 다중불포화지방산, 단일불포화지방산과 포화 지방산의 비 (P/M/S)가 DHA란은 변화가 없었고, 인삼란은 점차 증가하는 경향을 보였다. P/M/S비는 3주 저장한 일반란, 2주 저장한 인삼란과 0~3주 저장기간의 DHA란에서 적정권장 수준을 나타내었다.

• 동아시아식생활학회지
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• 제15권5
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• pp.582-590
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• 2005

The application of consomme prepared with different ratios of ground beef and ground chicken to improve taste preferences and the cost of foodstuffs was attempted by reviewing fundamental data and the effect on the sensory and physical characteristics of treatment These treatments were prepared with different ratios(9:1(A), 7:3(B), 5:5(C), 3:7(D), 1:9(E) w/w) of ground beef and ground chicken, respectively. Sensory properties were evaluated in the aspects of both acceptability and intensity. From the acceptability aspect, A treatment showed the highest scores in smell, B in mouthfeel and taste, A and B in color and overall acceptability, respectively. In terms of acceptability, B treatment was favored most for color, mouthfeel, taste and overall acceptability except smell. The more the ground chicken, the higher the intensity aspect E treatment showed higher values than A in all the intensity aspects. Moisture contents and crude proteins increased by increasing the mixed ratio of ground beef, whereas crude ash, solid matter and pH increased by increasing the mixed ratio of ground chicken. Tumidity values were the highest in E treatment, whereas the lowest in A. Tumidity values were increased significantly by increasing the mixed ratio of ground chicken. Viscosity increased by increasing the mixed ratio of ground chicken. The highest viscosity was shown in E treatment The colorimetric lightness values(L) decreased and redness(a), yellowness(b) and total color difference values(AE) increased significantly by increasing the mixed ratio of ground chicken. This experimental study showed that A and B treatment had the highest values in overall acceptability. The results of analysis in crude protein, solid matter, tumidity and viscosity were increased by increasing the mixed ratio of ground chicken. B treatment prepared with a ratio of 7:3 of ground beef and chicken had the most favorable sensory quality. These results suggested the basic information to improve acceptability and to save the costs of Consomme recipes for foodservice institutions.

• 대한물리의학회지
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• 제11권3호
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• pp.81-88
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• 2016

PURPOSE: This study was aimed at investigating the types of gender roles and the relationship between gender roles and job satisfaction among neurological physical therapists. METHODS: The study subjects were 169 (male 74, female 95) neurological physical therapists working at general hospitals or rehabilitation centers in the Daejeon Metropolitan City area, South Korea. To identify job satisfaction scores, the subjects completed a questionnaire, and a vernier caliper was used by a trained examiner to measure the lengths of the subjects' index and ring fingers (i.e., digitus secundus manus and digitus annularis, respectively) to examine gender roles. The index to ring finger length ratio (i.e., 2D:4D ratio) was calculated using a personal computer. An independent t-test was performed to compare the finger length ratio and job satisfaction of male group with that of the female group and a correlation analysis was performed to examine job satisfaction by gender roles. RESULTS: Finger length ratio is lower in males than in females. However, there was no significant difference statistically (p>.05). Regarding job satisfaction by gender, males were more satisfied with their jobs than females (p<.05). However, there were no significant correlations between job satisfaction and gender roles (p>.05). CONCLUSION: It cannot be concluded that bias against gender roles is a contributing factor for neurological physical therapists being satisfied with their job, and thus bias against gender roles among neurological physical therapists should be removed.

• 한국동물생명공학회지
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• 제34권3호
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• pp.222-231
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• 2019

Among fatty acid families, the polyunsaturated fatty acids were demonstrated to be mediators in various reproductive processes as precursor of steroid hormone (via cholesterol) and prostaglandins (via arachidonic acid), and in the last decade, major research was focused on the effects of omega-6 and especially omega-3 fatty acid. Eicosapentaenoic acid, the longest members of omega-3 fatty acid family, can be produced by a series of desaturation and elongation reactions from shorter member such as α-Linolenic acid. However, very few studies have provided detailed descriptions of Eicosapentaenoic acid effects and mechanisms of action in mammalian oocytes. The purpose of this study was to evaluate the effect of Eicosapentaenoic acid supplementation on in vitro maturation and developmental potential of porcine oocytes. Various concentrations of Eicosapentaenoic acid was added into in vitro maturation medium, and we evaluated the degree of cumulus expansion, nuclear maturation rate, blastocysts quality, and levels of prostaglandin E2, 17β-estradiol, progesterone in the spent medium. High doses (100 μM) of Eicosapentaenoic acid supplementation significantly inhibited cumulus expansion and oocyte nuclear maturation, and prostaglandin E2 synthesis also significantly decreased compared with other groups (p < 0.05). Supplementation of 50 μM Eicosapentaenoic acid showed higher quality blastocysts in terms of high cell numbers and low apoptosis when compared with other groups (p < 0.05), and synthesis ratio of E2/P4 also significantly increased compared with control group (p < 0.05). However, Supplementation of 100 μM Eicosapentaenoic acid showed high apoptosis when compared with other groups (p < 0.05), and synthesis ratio of 17β-estradiol/progesterone also significantly decreased compared with control group (p < 0.05). Our results indicated that supplementation with appropriate levels of Eicosapentaenoic acid beneficially affects the change of hormone synthesis for controlling oocyte maturation, leading to improved embryo quality. However, high doses of Eicosapentaenoic acid treatment results in detrimental effects.

• Bulletin of the Korean Chemical Society
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• 제15권7호
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• pp.559-563
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• 1994

Glycosylation of benzoylated glycosyl halides of glucose, galactose and mannose with potassium p-nitrophenoxide and 18-crown-6 complex in chloroform resulted in the stereospecific formation of 1,2-trans p-nitrophenyl glycopyranosides in good yields. The same reaction with benzylated mannopyranosyl chloride gave the ${\alpha}-and\;{\beta}-p-nitrophenyl$ mannopyranosides in 3 : 1 ratio. However, acetylated 2-azido-altropyranosyl chloride gave ${\beta}-p-nitrophenyl$ altropyranoside only.

• Clinical and Experimental Pediatrics
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• 제49권4호
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• pp.417-423
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• 2006

목 적 : 항암제와 방사선 치료의 개발, 골수나 조혈모세포 이식 기회의 증가로 소아 백혈병과 고형 종양을 가진 환아들이 장기 생존하게 되면서 치료에 따른 장기 부작용, 특히 심장 독성에 크게 관심을 가지게 되었다. 항암제에 의한 심장 독성을 파악하는데 가장 적절한 방법은 비침습적이면서 정량적으로 측정할 수 있는 심초음파라고 할 수 있다. 본 연구에서는 이미 소아암으로 진단을 받고 치료를 받는 중이거나 치료를 종료한 환아들을 대상으로 조직 도플러 영상을 사용하여 심실 기능을 평가하고, 향후 동질환의 환아에서 조기 및 후기 심독성을 조기에 발견하기 위한 방법으로 반복적인 조직 도플러 영상을 적용하는데 기여하고자 하였다. 방 법 : 2005년 4월 1일부터 7월 1일까지 계명대학교 동산의료원 소아 종양 크리닉을 방문한 항암치료 중이거나 항암치료 종료된 환아 23명을 대상으로 하였다. 전체 대상 환아군(1군)은 단축 분획 29% 이상(2군), 28% 이하의 군(3군)으로 나누고, anthracyclin 사용군(4군)과 미사용군(5군)으로 다시 나누었다. M-mode와 간헐파 도플러를 이용하여 단축 분획, 구혈률, 승모판막으로 유입되는 혈류의 E, A 속도, DT, IVRT를 측정하였고, 조직 도플러 영상을 이용하여 심첨 4, 2 방도에서 중격, 전벽, 하벽, 측벽의 기저부, 중간, 심첨 부위의 Sm, Em, Am 속도를 측정하였다. 측정한 값을 이용하여 modified Tei index, E/A, E/Em ratio를 구하였다. 결 과 : 환아의 평균 나이는 8년 4개월이었고 남아 12명, 여아 11명이었다. 항암제 중 anthracyclin을 사용한 환아(4군)는 13명이었고, 방사선 치료를 받은 환아는 4명이었으며 단축 분획이 28% 이하인 환아(3군)는 6명이었다. 대조군의 평균 나이는 6년 6개월이었고 남아 6명, 여아 3명이었다. 환아군과 대조군간에 성별, 나이에 있어서 유의한 차이는 없었다. 대조군과 1군의 E/Em ratio는 $7.06{\pm}1.64$, $6.46{\pm}1.85$로 두 군간에 P<0.05로 의미있는 차이가 있었으나 다른 측정값들은 유의한 차이가 없었다. 대조군과 2, 3, 4, 5군간에도 E/Em ratio는 P<0.05로 의미있는 차이가 있었다. 2, 3군과 4, 5군간에는 측정값들의 군간의 유의한 차이가 없었다. 결 론 : 좌심실 평균 이완기압을 반영한다고 알려져 있는 E/Em ratio에서 대조군과 항암 치료 군간에 차이가 확인됨으로써 항암 치료 이후의 심장 기능 부전은 수축기 기능 변화가 아닌 이완기 기능 변화가 먼저 오는 것을 알 수 있었다. 심장 이완기 기능을 평가하는 간헐파 도플러 측정값들에서는 두 군간에 차이가 없음으로 이완기 기능을 평가하는데는 조직 도플러 영상을 이용하는 것이 더 민감한 방법이었다. 따라서 항암 치료 이후에도 장기적으로 관심을 가지고 심장 기능에 대한 잦은 추적 관찰이 필요하겠고, 추적 관찰하는 방법으로는 조직 도플러 영상을 이용한 심초음파 검사가 비침습적이고 간편하며 유용한 방법일 수 있다.

• Journal of Plant Biotechnology
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• 제49권1호
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• pp.90-98
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• 2022

This study was performed to determine the optimal ratio for preparing an extract comprising the Ecklonia cava and Hizikia fusiformis complex as a therapeutic material for alleviating inflammatory respiratory diseases. First, to examine the optimal ratio for preparing the complex (SD-EH), Ecklonia cava and Hizikia fusiformis extracts were used; four extracts with different mixing ratios were prepared. The effects of the SD-EH extract on MUC5AC mRNA expression in PMA-treated NCI-H292 cells were analyzed; it was confirmed that the MUC5AC expression was significantly reduced after treatment with the SD-EHA-001 (E(100) : H(0)), SD-EHB-001 (E(90) : H(10)), SD-EHC-001 (E(80) : H(20)), and SD-EHD-001 (E(70) : H(30)) extracts. Western blotting was used to determine whether the SD-EH extract affects the expression levels of COX-2 and MMP-9 in PMA-treated A549 cells. The protein expression levels of COX-2 and MMP-9 were significantly lower (p < 0.001) in the cells treated with the SD-EHC-001 (E(80):H(20)), SD-EHD-001 (E(70) : H(30)), and SD-EHE-001 (E(60) : H(40) extracts than in the cells treated with PMA alone. The SD-EHC-001 (E(80) : H(20)) extract markedly downregulated the expression levels of MUC5AC, COX-2, and MMP-9. Therefore, the SE-EH extract may serve as a potential therapeutic agent for treating inflammatory respiratory diseases.