• Journal of Microbiology and Biotechnology
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• v.10 no.3
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• pp.367-374
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• 2000

The composition of dissolved gases and nutrients in a liquid medium were determined for establishment of the optimum conditions for in vitro culture of Helicobacter pylori. A microaerobic condition facored by the organism was prepared by adjusting the partial pressure of the gas, agitation speed, and viscosity of the medium. The gaseous concentrations were controlled by utilizing CampyPak Plus that reduced oxygen while augmenting carbon dioxide. Agitation of the broth facilitated the oxygen transfer to the cells, yet inhibited the growth at high rates. An increase of viscosity in the medium repressed the culture although this variable was relatively insignificant. The chemical constituents of the liquid broth were examined to establish an economic model for H. pylori cultivation. The microbe required a neutral pH for optimum growth, and yet was also able to proliferate in an acidic condition, presumably by releasing the acidity-modulating enzyme, urease. Cyclodextrin and casamino acid were investigated as growth enhancers in place of serum, while yeast extract unexpectedly inhibited the cells. A low concentration of glucose, the unique carbon source for the organism, increased the cell density, yet high concentrations resulted in an adverse effect. Under optimally dissolved gas conditions, the cell concentration in brucella broth supplemented with serum substitutes and glucose reached $1.6{\times}10^8$ viable cells/ml which was approximately 50% higher than that obtained in the liquid medium added with only cyclodextrin or serum.

• Journal of Astronomy and Space Sciences
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• v.9 no.2
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• pp.183-192
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• 1992

We have investigated the properties of dust grains in the envelopes of infrared carbon stars by testing various radiative transfer model spectra with different stellar and enveloped parameters. We have deduced a new opacity pattern for the dust grains reflecting both the experimental data and the model fitting with recent infrared observations. The best pattern we find is very similar to amorphous carbon with a slight modification that could be attributed to some unknown dust grain materials. Unlike oxygen-rich dust grains, the optical properties of carbon grains do not show any reasonable tendency of temperature dependence. We find that the Planck mean values of radiation pressure efficiency factors for the modified amorphous carbon are much larger than those for graphite.

• Journal of Korea Foundry Society
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• v.15 no.5
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• pp.477-482
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• 1995

A computer simulation model of various smelting process for melting waste sand was developed by using energy and material balance concept. This model can predict the coal, flux and oxygen consumption and the volume and temperature of off-gas. The major critical variables for smelting process can be explained by using the analysis of energy and material balance. The major conclusions were as follows; 1. The most important variables for smelting process were high post-combustion ratio, high heat transfer efficiency and refractory protection technology. 2. For saving energy in this smelting process, selection of raw materials i.e coal, flux are very important, espacially using of low volatile coal is very profitable. 3. The treatment cost of waste sand is high and environmental restriction is severe, in this reason we must be concerned in the treatment of waste sand by smelting process.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.1
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• pp.55.2-55.2
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• 2012

Silicate-carbon stars are characterized by oxygen-rich (O-rich) dust features despite their carbon-rich (C-rich) photospheres. While the origin of silicate-carbon stars has been a mystery ever since their discovery, the most widely accepted hypothesis is that the silicate-carbon stars have a low-luminosity companion and the O-rich material is stored in a circumbinary disk or a circumstellar disk even after the primary star becomes a carbon star. In order to study the properties of circumstellar dust envelopes of silicate-carbon stars, we perform radiative transfer model calculations using RADMC-3D with an axi-symmetric dust density distribution (a disk) as well as a spherically symmetric dust distribution. For various dust envelope models with different shapes and chemistry, we calculate the model spectral energy distributions (SEDs) and compare the model results with the observed SEDs of selected 5 silicate-carbon stars. The Circumstellar disk models are fairly well fitted with the observational data of 5 silicate-carbon stars. We find some evidences that the circumbinary disk model could be a better explanation for the origin of silicate carbon stars than the simple detached silicate dust shell model of the transition phase of the stellar chemistry.

• Journal of Aquaculture
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• v.7 no.4
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• pp.239-249
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• 1994

This study was conducted to elucidate mixing characteristics of the media to dertermine hydraulic model parameters of the three phase fluidized bed for the water quality management of aquaculture. The volume and H/D ratio of the reactor were 1.76 Liter and 10.9, respectively. The experimental results showed that variance $(\sigma^2)$ and dispersion number $(D/{\mu}L)$ were above 0.47 and $0.35\~\infty$, respectively. Therefore, the condition of the reactor was almost ideal complete mixing. The dependence of the overall oxygen-transfer coefficient $(K_{La})$ on each experimental variables through a multple linear regression was given by the following equation ; $$K_{La}\;=\;44.9(D_i/D_c)^{-0.4611}\;A_f^{0.8622}\;C_m^{-0.0746}\;d_p^{-0.4302}$$

• Journal of Microbiology and Biotechnology
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• v.21 no.4
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• pp.430-437
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• 2011

The effects of the agitation and aeration rates on the production of serratiopeptidase (SRP) in a 5-L fermentor (working volume 2-l) were systematically investigated using Serratia marcescens NRRL B-23112. The dissolved oxygen concentration, pH, biomass, SRP yield, and maltose utilization were all continuously measured during the course of the fermentation runs. The efficiencies of the aeration and agitation were evaluated based on the volumetric mass transfer coefficient ($K_La$). The maximum SRP production of 11,580 EU/ml with a specific SRP productivity of 78.8 EU/g/h was obtained with an agitation of 400 rpm and aeration of 0.075 vvm, which was 58% higher than the shake-flask level. The $K_La$ for the fermentation system supporting the maximum production (400 rpm, 0.075 vvm) was 11.3 $h^{-1}$. Under these fermentor optimized conditions, kinetic modeling was performed to understand the detailed course of the fermentation process. The resulting logistic and Luedeking-Piret models provided an effective description of the SRP fermentation, where the correlation coefficients for cell growth, SRP formation, and substrate consumption were 0.99, 0.94, and 0.84, respectively, revealing a good agreement between the model-predicted and experimental results. The kinetic analysis of the batch fermentation process for the production of SRP demonstrated the SRP production to be mixed growth associated.

• Journal of Electrochemical Science and Technology
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• v.14 no.4
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• pp.333-348
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• 2023

In this study, a 3D model of the proton exchange membrane fuel cell is established, and a new 3D baffle structure is designed, which is combined with the parallel flow field and then optimized by numerical simulation methods. The number of baffles and the cross-sectional trapezoidal base angle are taken as the main variables, and their impacts on the performance indexes of the cathode side are analyzed. The results show that the 3D baffle can facilitate the convection and diffusion mass transfer of reactants, improve the uniformity of oxygen distribution, enhance the drainage capacity, and make the cell performance superior; however, too small angle will lead to excessive local convective mass flux, resulting in the decrease of the overall uniformity of oxygen distribution and lowering the cell performance. Among them, the optimal number of baffles and angle are 9 and 58°, respectively, which improves the net output power density by 10.8% than conventional flow field.

• Journal of Power System Engineering
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• v.11 no.3
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• pp.22-28
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• 2007

Liquefied gas vaporizer is a machine to vaporize liquefied gas such as liquid nitrogen($LN_{2}$), liquefied natural gas(LNG), liquid oxygen($LO_{2}$) etc. For the air type vaporizer, the frozen dew is created by temperature drop (below 273 K) on vaporizer surface. The layer of ice make a contractions on vaporizer. The structure analysis on the heat transfer was studied to see the effect of geometric parameters of the vaporizer, which are length 1000 mm of various type vaporizer. Structure analysis result such as temperature variation, thermal stress and thermal strain have high efficiency of heat emission as increase of thermal conductivity. As the result, Frist, With-fin model shows high temperature distribution better than without-fin on the temperature analysis. Second, Without-fin model shows double contractions better then with-fin model under the super low temperature load on the thermal strain analysis. Third, Vaporizer fin can be apply not only heat exchange but also a stiffener of structure. Finally, we confirm that All model vaporizer can be stand for sudden load change because of compressive yield stress shows within 280 MPa on thermal stress analysis.

• Journal of the Korean Society of Marine Environment & Safety
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• v.26 no.5
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• pp.569-575
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• 2020

In this study, a fuel cell system model for ship power was developed and verified by comparing the experimental results obtained by supplying pure oxygen. To verify the proposed model, the fuel cell output characteristics when oxygen was supplied were compared with those when air was supplied using an air compressor. In addition, the effect of the change in the thermal properties of the fuel cell system on the output of the stack was examined. Within the experimental range of this study, when pure oxygen was supplied as the cathode supply gas, the calculated and experimental voltages and outputs obtained through modeling were almost the same over the entire load range. When air was supplied instead of oxygen for the cathode supply at a constant load of 560 A, each stack voltage was approximately 14 V, the stack output was approximately 8 kW, and the stack efficiency was approximately 3 %. It was confirmed that the overall system efficiency was reduced by approximately 8 %. Among the thermal properties examined in this study, the heat transfer coefficient of the coolant to the stack was found to have the greatest effect on the output of the stack.

• Bulletin of the Korean Chemical Society
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• v.34 no.9
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• pp.2629-2634
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• 2013

Fisetin is a naturally occurring flavonoid with some anti-cancer and anti-inflammation capabilities. In this study, we perform docking studies between human c-Jun N-terminal kinase 1 (JNK 1) and fisetin and proposed a binding model of fisetin and JNK 1, in which the hydroxyl groups of the B ring and oxygen at the 4-position of the C ring play key roles in binding interactions with JNK. Fluorescence quenching and saturation-transfer difference (STD) NMR experiments showed that fisetin exhibits good binding affinity to JNK, $1.32{\times}10^8M^{-1}$. The anti-inflammatory activity of fisetin was also investigated. Fisetin significantly suppressed tumor necrosis factor, the NO production, and macrophage inflammatory cytokine release in LPS-stimulated RAW264.7 mouse macrophages. We found that the anti-inflammatory cascade of fisetin was mediated through the JNK, and cyclooxygenase (COX)-2 pathways. Our findings suggest the potential of fisetin as an anti-inflammatory agent.