• 한국미생물·생명공학회지
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• 제32권2호
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• pp.149-153
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• 2004

븐 연구에서는 E. coli MT201을 이용한 L-threonine 발효공정을 확립하였다. 회분식 배양에서 L-threonine생산이 균체 증식과 더불어 증가하는 증식과 연관된 형태의 생산 곡선을 보여주었다. L-Threonine 생산에 대한 조건을 확립하기 위한 유가식 배양에서 첨가배지내 최적 효모추출물과 포도당의 농도는 각각 60 g/$\ell$와 600 g/$\ell$이였으며, 약 87 g/$\ell$의 L-threonine이 생산되었다. 유가식 배양에서도 L-threonine 생산은 회분식 배양과 마찬가지로 균체량의 증가와 더불어 L-threonine 생산이 증가하는 증식과 연관된 형태를 보여주었다. 이러한 결과를 바탕으로 적절한 균체량의 증가와 L-threonine 생산성 향상을 위하여 L-methionine과 인산염이 추가로 공급된 첨가배지를 이용한 고농도 배양 조건이 확립되었다. 한편 고농도 배양에 따른 용존산소의 결핍을 해결하기 위하여 산소가 포함된 혼합공기를 사용하였다. 최적화된 유가식 배양 결과 균체 증식도 원활하게 증가하였으며, L-threonine의 생산도 약 98 g/$\ell$로 향상되었다. 이때 L-threonine의 생산성은 약 3.85 g/$\ell$/h이었다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제17권5호
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• pp.10-19
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• 2012

The aim of this study was to examine chemical and isotopic compositions of groundwater and lake water near an area contaminated by petroleum and to evaluate influence of petroleum on them during the period from March to August 2011. In dry season, $Ca^{2+}$ and $SO{_4}^{2-}$ were dominant in the groundwater and lake water and $Ca^{2+}$ and $HCO{_3}^-$ were significant in wet season. ${\delta}^{18}O$ and ${\delta}D$ of the groundwater and lake water were plotted near LMWL (${\delta}D=8.06{\delta}^{18}O+12.5$). ${\delta}^{18}O$ and ${\delta}D$ of the lake water did not show seasonal variation. However, ${\delta}^{18}O$ and ${\delta}D$ of the groundwater were enriched in wet season compared with those in dry season because of influence of small ponds around wells where evaporation losses were slightly experienced. Redox condition of most lake water was oxidation environment in contact with the atmosphere during the study period. However, redox condition of groundwater was transitional environment in dry season and oxidation environment in wet season because of influence of contaminant such as petroleum. In some groundwater, the concentrations of $NO{_3}^-$ in some groundwater were less than 1 mg/L because of denitrification. Also, $NO{_3}^-$ showed positive correlation with $SO{_4}^{2-}$ and weak negative correlation with $HCO{_3}^-$, because of influence of denitrification.

• 한국화재소방학회논문지
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• 제20권4호
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• pp.72-76
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• 2006

고분자/점토의 nanocomposite는 고분자에 소량의 점토를 첨가해도 물리적, 기계적, 열적 특성이 증가하기 때문에 최근 이에 관련된 연구가 증가되고 있다. 특히 montmorillonite(MMT)와 같은 smectite 계열의 점토는 높은 종횡비, 판형의 층상구조, 경제성 때문에 산업적으로 이용가치가 많다고 할 수 있다. 본 연구에서는 PE/MMT nanocomposite는 고분자를 용융시킨 후 점토를 삽입하여 시편을 제조하였다. 나노입자의 구조는 XRD 및 TEM을 이용하여 확인하였고, 난연성은 LOI, 탄화층 생성량, 연기중량농도의 측정을 통하여 검토하였다. 또한 PE/MMT nanocomposite의 열적 안정성은 DTG-DTA 분석을 통하여 검토하였다. 그 결과 종래의 화합물보다 그 특성이 보강되었다. 난연성은 연소시 탄화층 형성에 의하여 증가 되었음을 확인할 수 있었다.

• Journal of Ginseng Research
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• 제45권1호
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• pp.108-118
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• 2021

Background: Korean ginseng (Panax ginseng Meyer) contains a variety of ginsenosides that can be metabolized to a biologically active substance, compound K. Previous research showed that compound K could be enriched in the red ginseng extract (RGE) after hydrolysis by pectinase. The current study investigated whether the enzymatically hydrolyzed red ginseng extract (HRGE) containing a notable level of compound K has cognitive improving and neuroprotective effects. Methods: A scopolamine-induced hypomnesic mouse model was subjected to behavioral tasks, such as the Y-maze, passive avoidance, and the Morris water maze tests. After sacrificing the mice, the brains were collected, histologically examined (hematoxylin and eosin staining), and the expressions of antioxidant proteins analyzed by western blot. Results: Behavioral assessment indicated that the oral administration of HRGE at a dosage of 300 mg/kg body weight reversed scopolamine-induced learning and memory deficits. Histological examination demonstrated that the hippocampal damage observed in scopolamine-treated mouse brains was reduced by HRGE administration. In addition, HRGE administration increased the expression of nuclear-factor-E2-related factor 2 and its downstream antioxidant enzymes NAD(P)H:quinone oxidoreductase and heme oxygenase-1 in hippocampal tissue homogenates. An in vitro assay using HT22 mouse hippocampal neuronal cells demonstrated that HRGE treatment attenuated glutamate-induced cytotoxicity by decreasing the intracellular levels of reactive oxygen species. Conclusion: These findings suggest that HRGE administration can effectively alleviate hippocampus-mediated cognitive impairment, possibly through cytoprotective mechanisms, preventing oxidative-stress-induced neuronal cell death via the upregulation of phase 2 antioxidant molecules.

• 산업식품공학
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• 제22권4호
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• pp.353-357
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• 2018

Previous studies have suggested that rice bran oil (RBO), an edible oil from the byproducts of rice milling, has anti-inflammatory effects in inflammation inducing macrophages, known as M1 subsets. Yet the effects of RBO on the counterpart M2 subsets, the "healing" macrophages, were poorly investigated to date. In this regard, recent studies on the molecular/cellular anti-inflammatory mechanisms of dietary components have demonstrated that mitochondrial respiration contributes to macrophage functioning. Therefore, the current study examined whether RBO regulates cytokine secretion by modulating mitochondrial metabolism in wound healing M2 subsets. Palm oil (PO), enriched with medium-chain fatty acids, served as a positive control. C57BL/6 mice were fed a diet containing either corn oil (CO), PO or RBO for 4 weeks, followed by purification of bone marrow-derived macrophages (BMDM) from their tibias and femurs. Cells were further polarized to M2-BMDM, and the expression of M2 marker (CD206) on cellular surfaces were not affected by dietary intervention. In addition, the secretion of anti-inflammatory cytokine (IL-10) in the culture supernatant was not affected by dietary lipids. Oxygen consumption rate, the indicator of mitochondrial respiration in M2-BMDM was not regulated by RBO intervention and PO treatment. Taken together, this study imply that RBO did not intervene both the regulation of inflammatory responses and mitochondrial respiration in M2 macrophages.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.197-197
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• 2022

Wheat is highly susceptible to heat stress, which significantly reduces grain yield. In this study, we used RNA-seq technology to analyze the transcript expression at three different time-points after heat treatment in three cultivars differing in their susceptibility to heat stress: Jopum, Keumkang, and Olgeuru. A total of 11,751, 8850, and 14,711; 10,959,7946, and 14,205; and 22,895,13,060, and 19,408 differentially-expressed genes (log2 fold-change > 1 and FDR (padj) < 0.05) were identified in Jopum, Keumkang, and Olgeuru in the control vs. 6-h, in the control vs. 12-h, and in the 6-h vs. 12-h heat treatment, respectively. Functional enrichment analysis showed that the biological processes for DEGs, such as the cellular response to heat and oxidative stress-and including the removal of superoxide radicals and the positive regulation of superoxide dismutase activity-were significantly enriched among the three comparisons in all three cultivars. Furthermore, we investigated the differential expression patterns of reactive oxygen species (ROS)-scavenging enzymes, heat shock proteins, and heat-stress transcription factors using qRT-PCR to confirm the differences in gene expression among the three varieties under heat stress. This study contributes to a better understanding of the wheat heat-stress response at the early growth stage and the varietal differences in heat tolerancea.

• 자원환경지질
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• 제23권2호
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• pp.245-259
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• 1990

Petrochemical analyses of granitic rocks including trace element, REE and oxygen isotope were carried out to understand petrogenesis of plutonic rocks from the Chungju, Wolaksan and Jecheon granite batholiths, which might be related with tungsten-base metal-fluorite mineralization in the Hwanggangri metallogenic province. Different geochemical characteristics such as major and trace elements were found between Jurassic Daebo granitic rocks (Chungju, Jecheon, Wonju, and Boeun granitic rocks) and Cretaceous Bulgugsa granitic rocks (Wolaksan, Muamsa and Sokrisan granitic rocks). Cretaceous granitoids are characterized by high $SiO_2$and $K_2O$ contents and low $TiO_2$, $Al_2O_3$, MgO and CaO contents. They also have relatively high contents of trace elements(Zn, V, Co, Cr, Sr, and Ba) in comparison with the Jurassic granitoids. (Eu)/($Eu^*$) and $(La/Lu)_{CN}$ ratios of Jurassic plutons vary from 0.78 to 1.13 and from 26.02 to 30.5, respectively, while the ratios of Cretaceous ones range from 0.22 to 0.28 and from 4.42 to 14.2, respectively. The REE patterns of the Cretaceous and Jurassic granitic rocks have quite different Eu anomalies: large negative Eu anomaly in the former, and mild or absent Eu anomaly in the latter. The large Eu negative of Cretaceous granitic rocks are interpreted as a differentiated product of fractional crystallization of granitic magma deduced by Rayleigh fractionation model(Tsusue et al., 1987). Oxygen isotopic compositions of quartz for Daebo and Bulgugsa granitic rocks range from 9.98 to 10.51‰ and from 8.26 to 9.56‰, respectively. The Daebo granitic rocks enriched in $^{18}0$ suggest that the magma be undergone different partial melting processes from the Bulgugsa ones. Of the Bulgugsa granitoids, Wolaksan and Sokrisan mass have different contents of trace elements and ${\delta}\;^{18}0$ values of the silicate minerals, which indicate that they are not from the identical source of magma. Many mineral deposits are distributed in and/or near the Wolaksan and Muamsa granitic rocks, but a few mineral deposits are found in and near the Chungju and Jecheon granite batholiths. It might be depend on geochemisty of the related igneous rocks which have low contents of Ba, Sr, Co, V, Cr, Ni, Zn and high contents of Nb and Y, and on lithology of country rocks such as cabonate and noncarbonate rocks.

• 한국산학기술학회논문지
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• 제18권7호
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• pp.662-669
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• 2017

군용 회전익 항공기는 임무 특성상 피탄 환경에 노출이 많이 되며, 피탄 후 연료누설에 의한 화재는 인명 손실로 직결된다. 피탄 시 조종사 및 승무원 생존성 향상을 위해 회전익 항공기 연료탱크는 내탄 성능과 내 폭발 성능이 요구된다. 내탄 성능은 자기밀폐성 셀을 연료탱크에 적용하여 만족시킬 수 있으며, 내폭발 성능은 폭발가능 영역의 산소 농도를 낮추는 방법과 열에 의한 연료의 증발을 최소화하여 가연성 연료 증기의 생성을 억제하는 방법을 통해 만족시킬 수 있다. 한국형 기동헬기는 피탄에 대한 내폭발 요구도를 만족시키기 위해 불활성가스발생장치를 통해 연료탱크 내 불활성 가스를 충진 시켜 산소농도를 낮추는 방법을 적용하고 있다. 본 논문에서는 국내 무기체계 양산단계의 부품국산화 업무 절차에 따라 개발된 불활성가스 발생장치의 개발 과정 전반에 대하여 기술하고 있다. 순수 국내 기술력을 통해 개발/제작 되었으며, 단품 성능 시험 및 항공기 장착 시험을 통해 기 장착되어 있는 제품 대비 동등이상의 성능을 가짐을 확인할 수 있었다.

• 대한기계학회논문집B
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• 제34권12호
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• pp.1101-1109
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• 2010

본 연구에서는 촤 연소 시에 분위기 가스 변화에 따른 연소 반응 변화를 수직 관형 연소로를 이용하여 실험적으로 측정해 보았다. 실험 조건으로 아역청탄인 Adaro탄과 역청탄인 Coal valley탄을 사용하였고, 두 탄을 $O_2/CO_2$조건과 $O_2/N_2$조건에서 온도를 900, 1100, $1300^{\circ}C$, 산소 분압을 12, 21, 30%로 변화를 주어 촤 반응성 실험을 수행하였다. 순산소 조건과 일반 대기 조건을 비교하였을 경우 순산소 조건일 때 입자온도와 총괄 반응량이 낮게 나타나는 경향을 보였지만, 입자 표면에서의 반응률을 계산한 결과, 분위기 가스의 영향과 상관없이 거의 동일한 경향을 가지는 것으로 나타났다. 또한 두 조건에서의 촤 연소 반응을 위한 반응 속도 상수 및 활성화 에너지 역시 분위기 가스의 영향과 상관없이 유사한 값을 가지는 것으로 확인하였다. 전반적으로 Adaro탄이 Coal valley탄보다 입자 온도와 총괄 반응량이 높게 나타났다.

• 한국해양학회지
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• 제7권1호
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• pp.1-14
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• 1972

낙동강은 유로연장 525km, 유역면적 23,852$\textrm{km}^2$에 달하는 남한 제1의 하천수계를 이루고 있고, 그 수원은 멀리 강원도 삼척군 삼장면 함백산에서 이루어져, 많은 지류수가 합류되어 경남 김해군 명지면과 부산시 서구를 거쳐 남해의 동부해역으로 유입된다. 하구일대는 우리나라에서도 굴지의 해태어장이 형성되고, 인근해역에서는 많은 어패류가 생산된다. 낙동강 하구수역의 수질에 관해서는 원(1964)이 간헐지 수질의 연간변화에 관한 조사가 이루어졌고, 또 원(1970)은 가덕도와 용원리 사이의 해역에서 3월 17-23일에 걸친 조석에 따른 영양염조사를 보고하였고, 최 외(1970)는 낙동강 하구일대의 해태어장개발을 목적으로 한 영양염, 유기현탁물, 조석류, 저질 등에 관한 일련의 결과를 보고하고 있다.