• Title/Summary/Keyword: Oxidoreductase

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NAD(P)H-quinone oxidoreductase-1 silencing modulates cytoprotection related protein expression in cisplatin cytotoxicity

  • Park, Se Ra;Jung, Ju Young;Kim, Young-Jung;Jung, Da Young;Lee, Mee Young;Ryu, Si Yun
    • Korean Journal of Veterinary Research
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    • v.56 no.1
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    • pp.15-21
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    • 2016

  • NAD(P)H-quinone oxidoreductase-1 (NQO1) is a down-stream target gene of nuclear factor erythroid 2-related factor 2 (Nrf2), and performs diverse biological functions. Recently, NQO1 is recognized as an effective gene for the cytotoxic inserts with its diverse biological functions, which is focused on antioxidant properties. The aim of present study was to assess the impact of NQO1 knockdown on cytoprotection-related protein expression in cisplatin cytotoxicity by using small interfering (si) RNA targeted on NQO1 gene. Cytotoxicity of cisplatin on ACHN cells was assessed in a dose- and time-dependent manner after siScramble or siNQO1 treatment. After cisplatin treatment, cells were subjected to cell viability assay, western-blot analysis, and immunofluorescence study. The cell viability was decreased in the siNQO1 cells (50%) than the siScramble cells (70%) after 24 h of cisplatin ($20{\mu}M$) treatment. Moreover, cytoprotection-related protein expressions were markedly suppressed in the siNQO1 cells after cisplatin treatment. The expression of Nrf2 and Klotho were decreased by 20% and 40%, respectively, of that in siScramble cells. Nrf2 and Klotho activation were also decreased in cisplatin treated siNQO1 cells, confirmed by cytoplasm-tonuclear translocation. Our findings demonstrate that the increased cisplatin-induced cytotoxicity was accompanied by suppressed Nrf2 activation and Klotho expression in siNQO1 cells.

  • https://doi.org/10.14405/kjvr.2016.56.1.15 인용 PDF

The Effects of Acupuncture at Heart and Kidney Meridian on Mitochondrial Respiratory Chain Complexes Activities in Rats (심경, 신경의 오수혈 침자가 Mitochondrial Respiratory Chain Complexes에 미치는 영향)

  • Choi, Donghee;Lee, Yumi;Kim, Mirae;Park, Jeonghye;Kim, Hyeran;Na, Changsu;Youn, Daehwan
    • Korean Journal of Acupuncture
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    • v.37 no.1
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    • pp.37-45
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    • 2020

  • Objectives : Mitochondria are typically known as intracellular double membrane-bound structures that supply energy for intracellular metabolism including Krebs cycle and beta-oxidation. Also, acupuncture has been known to stimulate the flow of energy. To explore the effect of acupuncture on the mitochondrial respiratory chain activities in rat's heart and kidneys, the activities of mitochondrial respiratory chain complexes I to IV were observed. Methods : The rats were divided into 11 groups; Normal (no acupuncture treatment and under anesthesia for 10 min), heart meridian five-transport-points (acupuncture treatment at HT9, HT8, HT7, HT4 and HT3 under anesthesia for 10 min), and kidney meridian five-transport-points (acupuncture treatment at KI1, KI2, KI3, KI7 and KI10 under anesthesia for 10 min). All rats were sacrificed and the heart and kidneys were examined for the changes of respiratory chain activities. Results : Acupuncture at HT7 increased the activity of succinate dehydrogenase; acupuncture at KI2 increased the activity of ubiquinol cytochrome C oxidoreductase; and acupuncture at HT9, HT8, HT3 and KI1 increased activities of cytochrome C oxidase. Conclusions : Acupuncture assists mitochondrial repiratory chain activity via the Cytochrome C oxidase signaling pathway in heart and kidney of rats.

  • https://doi.org/10.14406/acu.2020.006 인용 PDF KSCI

Simulation of Ammonia Reduction Effect by Hydroxylamine-oxidoreductase Enzyme Immobilized on the Surface of Water Pipe (수로관 표면 고정 히드록실아민-산화환원효소에 의한 암모니아 저감 효과 모사)

  • Lee, Sang-Ryong;Park, Jin-Won
    • Journal of the Korea Organic Resources Recycling Association
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    • v.28 no.4
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    • pp.35-41
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    • 2020

  • The immobilization of the hydroxylamine-oxidoreductase on the water channel surface was performed to investigate the efficacy of ammonia removal in turbulent flow. The reaction by this enzyme proceeds rapidly by converting hydroxylamine into nitrous acid. For the analysis of the effect, a dimensionless mass transfer governing equation was established with the physical properties based on room temperature. The ammonia diffusion coefficient in water and the kinematic viscosity coefficient of water were 2.45×10-9 ㎡/s and 1×10-6 ㎡/s, respectively. The distribution of ammonia concentration in the water was calculated with respect to the distance from the point at which exposure to ammonia began. The quantitative distribution with respect to the mixing depth was also found. Such a quantitative analysis can provide insight into whether the enzyme immobilized on the water channel surface can be effectively used for ammonia removal.

  • https://doi.org/10.17137/korrae.2020.28.4.35 인용 PDF KSCI