• Korean journal of applied entomology
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• v.42 no.3
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• pp.241-248
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• 2003

The aphid-eating gall-midge, Aphidoletes aphidimyza (Rondani), as predator of aphids was wildly used as one of the biological control agents for control of several kinds of aphids. Their some kinds of biological characteristics were examined. The developmental periods of A. aphidimyza from egg to adult with the green peach aphid as prey were 40 and 12 days at 15$^{\circ}C$ and 30$^{\circ}C$, respectively. Supplying the cotton aphid as prey, their developmental periods were shorter than with the green peach aphid. In case of the preservation of pupa in the cold condition, emergence rates were over 90% for 1 and 2 weeks at 5$^{\circ}C$ and l0$^{\circ}C$, respectively. Adult females of A. aphidimyza began mating and laying eggs at 2-3 days after emergence, and they laid about 200 eggs for lo days of average life span. Most of adults were emerged from pupa at 6 to 8 pm during a day, and they mainly acted in the early night.

• Korean Journal of Environmental Biology
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• v.40 no.2
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• pp.187-198
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• 2022

In this study, Telenomus remus Nixon (Hymenoptera: Scelionidae) was first reported as a natural enemy of the fall armyworm Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) egg collected from corn fields in Korea, and its biological characteristics was studied. Based on morphological and molecular analysis, the parasitoid emerged from S. frugiperda eggs was identified as T. remus. We found that T. remus can attack the eggs of S. frugiperda, Spodoptera litra and Spodoptera exigua under a laboratory condition. The longevity of T. remus female adult was longer than that of male adult. The egg-to-adult period of T. remus was not affected by the host age and sex. T. remus female adult laid at least 1-3 eggs a day to a maximum of 37 or more eggs, and the most oviposited on the 3^rd to 4^th day after emergence. The host preference for oviposition of T. remus adult was high in the order of S. litura>S. exigua>S. frugiperda. T. remus preferred to parasitize 1- and 2-day-old host egg rather than 3-day-old host egg. When compared to the sex ratio of T. remus progeny, the rate of female progeny was higher at the initiation time of oviposition, while the proportion of male progeny increased significantly with female adult age, especially after 8-day-old adult. This information may be useful for improving T. remus mass rearing system and developing a biological control program to control S. frugiperda.

• Korean journal of applied entomology
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• v.58 no.4
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• pp.381-387
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• 2019

From 2017 to 2019, the ovipositional and the developmental characteristics of Gampsocleis ussuriensis Adelung in the Buan area of Jeonbuk Province were examined. G. ussuriensis were mostly found in the weeded areas around the reservoir, where the adults first appeared in mid-July, showed up where by the early September, and overwintered in eggs. Nymphs appeared in early April to mid-July the next year. The nymphs hatched from early April and adults appeared after molting five times. The ovipositional period of G. ussuriensis was approximately 58 days. The total number of eggs per female was 124. The mean longevity of adults was 95.6 days for females and 84.8 days for males. Ovipositional mats were best with mixed Masato and Coco-Pitt at a ratio of 7:3. Developmental period of G. ussuriensis nymphs was 64.1 days at 24℃ and was longer than at different temperatures. The higher the temperature, the shorter the developmental period. The survival rate of nymphs was the best at 32℃ in 77.8%. The higher the density while rearing, the lower the survival rate, and the faster the development and molting velocity.

• International Journal of Industrial Entomology and Biomaterials
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• v.26 no.2
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• pp.124-130
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• 2013

Bumblebees are widely used to pollinate various greenhouse crops. Among the different bumblebee species, Bombus ignitus is indigenous to Korea, China, Japan and Russia. B. ignitus undergoes one generation per year, and artificial hibernation is essential for year-round rearing of the bumblebee. Keeping the queens under low-temperature conditions for several months is an effective method for terminating their diapause and promoting colony development. In the present study, we investigated how cold temperature affects the artificial hibernation of B. ignitus queens. Under chilling temperatures of $-2.5^{\circ}C$, $0^{\circ}C$, $2.5^{\circ}C$ and $5^{\circ}C$ with constant humidity >80%, the queens stored at $2.5^{\circ}C$ exhibited the highest survival rates, which were 74.0% at one month, 67.0% at two months, 60.0% at three months, 46.0% at 4 months, 33.0% at 5 months and 24.0% at 6 months. Lower survival rates were observed at $0^{\circ}C$, $5^{\circ}C$, $7.5^{\circ}C$ and $12.5^{\circ}C$. At $2.5^{\circ}C$ the colony developmental characteristics after diapause were 1.2- to 1.5-fold greater than those when queens were stored at $5^{\circ}C$. Thus, $2.5^{\circ}C$ and 70% R.H. were the most favorable chilling temperature and humidity conditions for terminating the diapause of B. ignitus queens.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.11
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• pp.1503-1509
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• 2010

Forty-eight 40-wk-old Hi-sex laying hens were individually caged in an environmentally controlled house to evaluate the effect of garlic (Allium Sativum) juice administration on egg production, egg quality, and yolk cholesterol. Garlic juice was prepared by blending pealed garlic cloves with distilled water (1:1, w/w). Hens were randomly divided into four equal groups; one served as a control and the other three groups were individually gavaged, 3.75 ml, 7.5 ml, or 15 ml garlic juice, three times a week, which respectively represented 0.25, 0.50 and 1% of body weight. Egg production was recorded on a daily basis; egg weight, albumen height, albumen and yolk pH, Haugh unit, and bacterial count of E. coli-challenged eggs were recorded at day of oviposition (day-1) and after 5 and 10 days of storage at room temperature. Yolk cholesterol content was analyzed for five successive weeks. Garlic juice increased (p<0.05) egg weight and mass with no change in egg production intensity. Garlic juice administration recorded higher (p<0.05) albumen height and improvement in Haugh unit. Also, eggs from garlic-treated hens recorded lower (p<0.05) albumen and yolk pH when compared to eggs collected from control hens. Garlic reduced (p<0.05) the $log_{10}$ of bacterial count in egg contents linearly when challenged with E. coli. Egg-yolk cholesterol content was not influenced by garlic juice administration. It is concluded that garlic juice improved performance characteristics and may increase egg shelf life as indicated by egg quality improvement and lower bacterial count of E. coli-challenged eggs. The levels of garlic juice used in this study were insufficient to influence egg yolk cholesterol.

• Parasites, Hosts and Diseases
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• v.55 no.2
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• pp.193-196
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• 2017

This study investigated the development characteristics of Dermacentor everestianus under laboratory conditions. The time taken for D. everestianus to complete the whole life cycle was 110.2 days on average, and the average developmental durations of larvae and nymphs were 17.1 days and 29.5 days, respectively. The summation of the prefeeding, feeding, and preoviposition periods of females was 17.8 days, and the oviposition and egg incubation lasted for 18.1 days and 27.7 days, respectively. A highly positive correlation was observed between the weight of engorged female and the number of egg mass laid (r=0.947). The reproductive efficiency index and the reproductive fitness index were 7.1 and 6.1, respectively.

• Korean journal of applied entomology
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• v.38 no.2
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• pp.101-107
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• 1999

Queens of Korean native bumblebee species, Bombus ignitus were collected from the field in the spring of 1998 to investigate their ecological characteristics and reared under the laboratory conditions; 3 0 f 1$^{\circ}$C, 55 25% of relative humidity and darkness. As a result, 94.4% of the queens laid eggs in 6.00f 2.41 days after the field collection. The first worker and the first drone from the egg cell of the collected queens appeared in 18.90 f 1.16 and 68.96 f 3.94 days, respectively. In the colony foundation, 86.1% of the collected queens founded their colony, and 96.8% of them produced new queens in 71.14f 6.88 days. The life span of the colony-founded queen covered for 86.90f24.35 days, whereas that of non-colonized queen for 15.67 f 7.37 days. One colony sized as big as 987.82f 145.84 in number, composing of workers (188.79f 18.23), drones (691.10f 159.24) and queens (107.93 f47.48). Copulation lasting time of queen and drone took 23.00f 7.33 minutes in average. In a new queen emergence, there were two patterns; single peak type of new queen emergence in 12.31 k6.95 days and two peaks type of it in 74.00f 17.61 days. The number of eggs per egg cell of new queen averaged as 8.86f 4.00 grains and an egg period covered 3 days after oviposition. An egg cell sized as 7.15 f0.88 mm in a width, 7.79 f 1 .l 1 mm in a length and 3.33 f 0.23 mm in a height. An egg shaped as banana and sized as 1.22 f 0.09 mm in a width and as 3.5 1 f 0.21 mm in a length, weighing as 2.70 + 0.30 mg.

• Korean journal of applied entomology
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• v.60 no.2
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• pp.175-183
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• 2021

As a baseline study of (common name) Locusta migratoria (Orthoptera: Acrididae) to utilize as a food source, we investigated the developmental characteristics of ovaries and eggs. Locusta migratoria had a pair of ovaries and more than 90-104 panoistic ovarioles. The ovary length in the adult stage was longer than it is in the 5th nymph stage. The length on the first day of the adult stage was 2.5-fold longer than the first day of 5^th instar. The ovary length showed a tendency to increase until the 30th day of the adult phase, but decreased from the 35^th day. Ovarioles length was about half of the ovary length, showing a similar tendency to the variation of ovary length. The lengths of the ovaries and the ovarioles increased proportionally to body weight. The matured eggs were identifiable from the 15^th day of the adult stage, and the number was the highest on the 30^th day at 50.6 individuals. Spermatheca size was also affected by the ovary development. The ovaries of L. migratoria were highly matured until the 30th day of adult stage. Weight and size of eggs increased sharply from the 4th day after egg oviposition and remained from the 7th day after spawning. Based on the above results, we found that the ovary of L. migratoria was most developed on the 30th day of adult phase, and 1st nymph is being hatched on the 10th day of the oviposition.

• Korean journal of applied entomology
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• v.61 no.3
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• pp.423-434
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• 2022

The two previously developed artificial diets (N4 and N6) used for rearing Spodoptera frugiperda (Noctuidae) larvae, were selected as highly-fit ones for rearing Mythimna loreyi larvae. Almost all biological characteristics were not significantly different between the colonies reared on the two diets at 25℃ and 15:9 h (light:dark) photoperiod. The developmental periods were 4.9-5.2 days for eggs, and 22.3-23.2 days for larvae. The pupal period and weight were different between the sexes in each diet colony. The pupal periods in females and males showed 12.6-12.8 days and 14.1-14.5 days, respectively. The pupal weights were ca. 345 mg for females and ca. 380 mg for males. The pupation and emergence rates were ca. 91-94%, and ca. 91-95%, respectively, without significant differences between the two colonies. The pre-oviposition and oviposition periods were 3.4 days and 4.7-4.8 days, respectively. The adult longevity was 8.2 days in females and 10.3-12.4 days in males. Total offsprings produced were found to be 724-847 larvae on an average with ca. 1,400 maximum larvae. In the life table analysis, the intrinsic rates of increases (0.1181 for N4 and 0.1253 for N6) were not significantly different between the two colonies. Individual differences in the larval instar number 5 and 6 were found within a diet colony. The ratios of 5-instar larvae were ca. 22% in N4 colony and ca. 7% in N6 colony. The larval period of 6-instar larvae was longer than that of 5-instar larvae. Width of head capsule in larvae varied from ca. 309 ㎛ for 1st instar to ca. 3,065 ㎛ for 6th instar. Body lengths measured from ca. 2.0 mm for 1st instar to ca. 29.1 mm for 6th instar. Larvae of M. loreyi and M. separata were found at the same time in a maize field during June and July, 2020.

• Korean journal of applied entomology
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• v.46 no.1 s.145
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• pp.51-56
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• 2007

The two species of rice bugs causing pecky rice, Cletus punctiger and Cletus schmidti are often observed coexisting in the rice fields of nearby fallow land. Direct feeding damage to rice by C. punctiger and C. schmidti can lead to a reduction in grain quality and quantity. These studies were carried out to investigate the development of C. punctiger and C. schmidti at various constant temperatures ranging from 20 to $30^{\circ}C$, 65% RH, and a photoperiod of 16L:8D. Egg hatchability of C. punctiger/C. schmidti at the temperatures of 20, 25 and $30^{\circ}C$ were 80.6/88.0, 91.7/96.3, 96.4/96.2%, respectively. The development periods of eggs of C. punctiger/C. schmidti at the temperatures of 20, 25, and $30^{\circ}C$ were 16.4/18.4, 9.4/10.2 and 6.4/7.3 days, respectively. Mean developmental periods of 1 st, 2nd, 3rd, 4th and 5th nymphs of C. punctiger/c. schmidti at $30^{\circ}C$ were 2.1/2.0, 3.5/4.0, 3.3/5.6, 3.2/4.8 and 5.8/6.9 days, respectively. Oviposition began 8.1 days after emergence at $25^{\circ}C$, and the longevity of female and male were 120.0 and 117.3 days, respectively. Total number of eggs through the life of female were 245.5 laying 2.2 eggs a day in average at $25^{\circ}C$. The development periods of egg and nymphs of C. punctiger were relatively shorter than those of C. schmidti. Availability of male had affected the egg hatchability greatly that laid at 30th day after 60 days period of oviposition in the presence of adult male of C. punctiger. The fertile eggs laid by the female together with male was 92.1% but those without male was only 9.6%.