• Journal of Crop Science and Biotechnology
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• v.11 no.4
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• pp.237-242
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• 2008

A liguleless mutant, which showed complete loss of lamina joint region at the junction between leaf blade and leaf sheath, was isolated from a Ds insertional mutants derived from the source cultivar, Dongjin. This mutant could not affect other developmental patterns like phyllotaxis. Southern blot analysis, using GUS as a probe, revealed that the liguleless mutant contained three Ds copies transposed in the rice genome. Among the four genomic sequences flanking the Ds, one was mapped in the intergenic region (31661640 - 31661759), and the other two predicted a protein kinase domain (12098980 - 12098667) as an original insertion site within a starter line used for massive production of Ds insertional mutant lines. Another predicted and inserted in first exon of liguleless 1 protein (OsLG1) that was mapped in coding region (LOC_Os04g56170) of chromosome 4. RT-PCR revealed that the OsLG1 gene was not expressed liguleless mutants. Structure analysis of OsLG1 protein revealed that it predicted transcription factor with a highly conserved SBP domain consisting of 79 amino acids that overlapped a nuclear localization signal (NLS). RT-PCR revealed that OsLG1 is mainly expressed in vegetative organs.

• Journal of the Korean Society of Safety
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• v.34 no.4
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• pp.111-118
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• 2019

Accidents occurring due to natural disasters in chemical process facilities where technologies are concentrated can cause secondary damage. The concept of the relationship between natural disasters and highly intensive technologies has evolved into the Natech (Natural Hazards Triggered Technological Disaster) research. Currently, the number of earthquakes is increasing all over the Korean peninsula. To assess the risk of Natech when an earthquake has occurred in South Korea, the Rapid Natech Risk Assessment Tool (RAPID-N) developed by the European Commission's Joint Research Center (EC JRC) was used in the present study. The RAPID-N can be used for Natech risk assessment based on mapping and can be utilized for sufficient preparation for reduction of the effects of Natech accidents. A total of 261 chemical facilities actually existing in Pohang were initially analyzed to select eight facilities and the Pohang earthquake that occurred in 2017 was implemented in the RAPID-N utilizing the shake map. High risk areas were selected through Natech risk assessments for the selected eight work places and countermeasures for the areas were suggested. High risk areas exist depending on the location, since the damage influence ranges could be overlapped and each chemical facility has an independent probability of Natech. Therefore, studies on Natech emergency plans and emergency evacuation routes should be actively conducted considering such high risk areas. The present study was conducted to demonstrate the feasibility of Natech risk assessment in South Korea through the RAPID-N. These findings can be used as a reference material to lay a foundation for Natech risk assessment and related policies in South Korea.