• Clinical and Experimental Reproductive Medicine
• /
• 제45권4호
• /
• pp.183-188
• /
• 2018

Objective: The purpose of this retrospective study was to evaluate the appropriateness of various follicle-stimulating hormone (FSH) starting doses in expected normal responders based on the nomogram developed by La Marca et al. Methods: A total of 117 first in vitro fertilization cycles performed from 2011 to 2017 were selected. All women were expected normal responders and used a recombinant FSH and flexible gonadotropin-releasing hormone antagonist protocol. The FSH starting dose was empirically determined (150, 225, or 300 IU). The FSH starting dose indicated by La Marca's nomogram was determined using female age and serum $anti-M{\ddot{u}}llerian$ hormone or basal FSH levels. If the administered dose was exactly the same as the proposed dose, the cycle was assigned to the concordant group (34 cycles). If not, it was assigned to the discordant group (83 cycles). Optimal ovarian response was defined as a total of 8-14 oocytes, hypo-response as < 8 oocytes, and hyper-response as > 14 oocytes. Results: Between the concordant and discordant group, ovarian response (optimal, 32.4% vs. 27.7%; hypo-response, 55.9% vs. 54.2%; and hyper-response, 11.8% vs. 18.1%) and the number of total or mature oocytes were similar. Ovarian hyperstimulation syndrome was rare in both groups (0% vs. 1.2%). The implantation rate, clinical pregnancy rate, miscarriage rate, and live birth rate were all similar. Conclusion: The use of the proposed FSH starting dose determined using La Marca's nomogram did not enhance the optimal ovarian response rate or pregnancy rate in expected normal responders. Individualization of the FSH starting dose by La Marca's nomogram appears to have no distinct advantages over empiric choice of the dose in expected normal responders.

• Asian-Australasian Journal of Animal Sciences
• /
• 제9권6호
• /
• pp.737-741
• /
• 1996

Dry matter yield of reed canarygrass was investigated in uncultivated rice paddy in Korea, and an attempt was made to estimate the most economic and efficient cutting frequency and rates of nitrogen (N) fertilization, for increased production of reed canarygrass. Total dry matter yields of reed canarygrass per year were 7.4-15.7, 8.5-16.1, and 7.5-13.4 tons/ha in 3, 4, and 5 cutting frequencies, respectively, and over the N treatments of 0-120 kg N/ha/cut. When cut 3 or 5 times annually, the 2nd cut produced the highest proportion of total yield at 38.4 and 33.0%, respectively, when cut 4 times the 3rd cut was highest (38.3%). The ranges of economic N level, limiting N level and efficiency of dry matter production were 243.3-293.0, 387.2, and 14.6 kg DM/kg N, respectively. These indicators were particularly low for the 5 cutting frequency possibly because of the unusually high temperature in the summer season. The best cutting frequency for the dry matter production of reed canarygrass in 1994 was 3 per year because of the higher efficiency of dry matter production.

• 한국수산과학회지
• /
• 제50권3호
• /
• pp.296-301
• /
• 2017

We conducted a study to 1) determine the indicators of gonadal maturity in male and female Korean walleye pollock Theragra chalcogramma for the purposes of artificial insemination; 2) establish the RNA/DNA ratio of mature eggs in this species; and 3) monitor the effect of refrigerated storage on egg viability and the motility of sperm collected from dead adult males. During the spawning season, the color of female gonads changed from orange to transparent, and that of male gonads changed from pale orange to milky white. The DNA content and RNA/DNA ratio of mature eggs were maintained without significant changes for approximately 6 h when eggs were preserved at $4^{\circ}C$. Sperm could be obtained from both milt and undiluted semen. Sperm obtained from milt ceased moving on the second day after isolation, while over 60% of sperm obtained from semen showed movement until the 13th day. Seven attempts were made to artificially inseminate mature eggs, of which two resulted in successful fertilization. The successful inseminations produced 94,000 and 5,000 fertilized eggs, respectively. This study shows that artificial insemination of walleye pollock is a viable strategy when natural propagation is not possible.

• The Korean Journal of Ecology
• /
• 제17권4호
• /
• pp.463-469
• /
• 1994

For better understanding of population dynamics of Symplocarpus renifolius, some aspects of seed production were studied in natural populations for 3 years. The rate of reproducing plants (RP) was 8.06% among the whole studied. The RPs were 0.0% in leaf size class under 500cm2 per individual, and 3.6% in 500~1,000cm2, and 44.3% in over 3,000cm2. The resource allocated to sexual organ was 11.6% of total biomass at the end of growing season, and that to belowground was about 80% regradless of presence or absence of sexual organ. In the previous and the next years of seed production, the energy allocated to sexual organ didn't affect the changes of leaf size, year by year. After flowing season. Especially, a large number of sex organ were degraded in April, a period of pollination and fertilization. The number of seeds per individual was degraded in April, a period of pollination and fertilization. The number of seeds per individual was 13~22 and didn't relate to leaf size. But the weight per seed increased along leaf size per individual. Therefore, in S.renifolius population, large individual produced large seeds rather than many seeds.

• The Korean Journal of Ecology
• /
• 제26권2호
• /
• pp.71-74
• /
• 2003

Effects of nitrogen addition on the growth of Indigofera pseudo-tinctoria (Leguminosae) in the waste landfill site was investigated. Nitrogen fertilization in the nitrogen poor soils of waste landfill may influence the growth of nitrogen fixing plants beneficially or detrimentally. When I. pseudo-tinctoria was fertilized with three different levels of nitrogen, the coverage of plants treated with 46 g N/$m^2$ and 460 g N/$m^2$ was significantly less than that of plants treated with 23 g N/$m^2$. The growth rates of plant height treated with 46 g N/$m^2$ and 460 g N/$m^2$ were significantly less than those of plants treated with 23 g N/$m^2$. The growth rates of plant diameter treated with 46 g N/$m^2$ and 460 g N/$m^2$ were significantly less than those of plants treated with 23 g N/$m^2$. Dry weights of whole plants in control sites were higher than those of all the others nitrogen treatment sites. Nodule numbers were higher in control plants than those of plants in all the other nitrogen treatment sites. It is suggested that nitrogen fertilizer addition over 23 g N/$m^2$ affect the growth of some nitrogen fixing plants, such as I. pseudo-tinctoria, negatively.

• 한국초지조사료학회지
• /
• 제12권2호
• /
• pp.104-110
• /
• 1992

This experiment was carried out to determine the effects of nitrogen(N) fertilization levels(0. 120, 240 and 360 kg/ha/y) on seasonal nitrate nitrogen($NO_3$-N) concentration and water soluble carbohydrate (WSC) content of grasses grown under shading condition. The plants were sampled on 7. 14, 21, 28 and 35 days after harvesting in spring(May), summer(July) and autumn(September), 1988. respectively. Shading degree was controlled artificially ca. 45-50%, and each annual level of N was distributed 5 times equally. The $NO_3$-N concentration of grasses was very low(0.064 %) in spring, high in summer(O.l98 %) and autumn(0.234%), and NO,-N was slightly decreased with growing of grasses. Also the NO,-N concentration of grasses was increased significantly with increasing level of N. In spring, the $NO_3$-N of grasses was very safety(less than 0.1 %), regardless of plant height and N level. In summer and autumn, however, plant heights of below 40 cm(summer) and 44 cm(autumn), and N levels of over 210 kg(summer) and 140 kg(autumn) were over toxic level to animals, respectively. The WSC content of grasses was slightly decreased with grass growth. and significantly decreased with high level of N. From the above results, it is suggested that 200 kg/ha of N in this experiment is effective for forage quality. Also a little more amount of N(50-70kgltimes) in spring. and low level of N(20-30 kgltimes) in summer and autumn season may be desirable on woodland posture.

• 한국수정란이식학회지
• /
• 제12권1호
• /
• pp.111-116
• /
• 1997

The present study was carried out to produce in vitro fertilized embryos with immature follicular oocytes collected by transvaginal aspiration from Holstein cows. A simple aspiration apparatus consists of two stainless steel tubes, an inner tube (needle holder; 1.2cmdiameter, 55cm long) and an outer tube (1.5cm diameter, 4Scm long), and a hand-operated vacuum pump was used. Under epidural anesthesia, the needle guide was passed into the vagina of the cow to a point next to the cervix. An ovary was placed against the wall of the vagina over the end of the aspiration needle by rectal manipulation. As the needlepassed into the ovary, an assistant was asked to apply vacuum(l00mrnHg) and the ovary was manipulated back and forth in all directions over the needle. When all sites of the ovary was aspirated, the needle was withdrawn and the needle guide was moved to the other side of ovary and the procedure was repeated. When the oocyte aspiration procedure was finished, collected fluid was transported to laboratory. Oocytes surrounded with at least 1 layer of cumulus cells were matured, fertilized and cultured in vitro. The results were as follows; Ninety seven oocytes were collected by transvaginal aspiration from seventeen Holstein cows(5.7 /head). The number of oocytes surrounded with at least 1 layer of cumulus cells were 60(61.9%). Following in vitro maturation, fertilization and culture, the cleavage and development rate to morula+blastocyst were 83.3% and 30.0%, respectively. From this study, transferable in vitro fertilized embryos could be produced with imma- ture follicular oocytes collected by transvaginal aspiration from Holstein cows using a simple aspiration apparatus.

• 한국가축번식학회지
• /
• 제16권3호
• /
• pp.199-208
• /
• 1992

These studies were carried out to investigate optimal physological conditions for in vitro fertilization (IVF) of mouse ova. The unfertilized ova were obtained by superovulation from ICR mice of 4 to 6 weeks old. Tyrode's 280 solution was used as basal media, and pH and osmolality of basal media were adjusted with the supplementation of sodium bicarbonate and sodium chloride, respectively. The optimal pH, and osmolality of culture media and the optimum period of sperm preincubation were examined in fertilization in vitro of mouse ova and the subsequent culture in vitro of embryos. The pH range of media examined was designed from 6.5 to 7.5 with 0.2 interval and the range of osmolality from 250 to 370 mOsm with 20 interval, and the period of sperm preincubation examined was 30, 60, 120, and 180 minutes. The ova developed to 2-cell embryosafter 26hrs. of incubation with preincubated sperm were evaluated as in vitro fertilized ones. The results obtained were summarized as follows: 1. The percentage of in vitro fertilized ova was highest (64.7%) in media of pH 7.1 and lowest (38.0%) in pH 6.7. No significant difference in % fertilized ova was found from the media of pH 7.1 to 7.5. Compared with the result from pH 7.1 medium, the pollyspermy was increased signifciantly (p<0.05) in the media of pH over 7.5 and below 6.9;, and the % degenerated ova was significantly (p<0.05) increased in the media of pH below 6.9. 2. The percentage of in vitro fertilized ova was highest (69.4%) in media of osmolality 330 mOsm and lowest (47.9%) in osmolality 250 mOsm. No significant difference in % fertilized ova was found from the media of osmolality 310 to 350 mOsm. Compared with the result from osmolality 330 mOsm in medium, the polyspermy aws increased significantly(p<0.05) in the media of osmolality over 350 mosmol and blow 290 mOsm, and the % degenerated ova was significantly (P<0.05) increased in the media of osmolality below 290 mOsm. 3. The percentate of in vitro fertlilized ova was highest (62.7%) in media of period sperm preincubation 180 min. and lowest (40.4%) in sperm preincubation 30 minutes. No significant difference in % fertilized ova was found from the media of sperm preincubation 120 to 180 minutes. Compared with the result from sperm preincubation 180 minutes in medium, the polyspermy was low differ no significantly(P<0.05) in the media of period sperm preincubation, and the % degenerated ova was signifciantly(P<0.05) increased in the media of sperm presincubation below 60 minutes.

• 생물환경조절학회지
• /
• 제20권4호
• /
• pp.357-364
• /
• 2011

능유바위솔을 분화로 재배하고자 할 때, 적정 광도 와 내음성정도, 적정 분용토, 그리고 적정 시비조건을 알아보기 위해 실험을 수행하였다. 그 결과 능유바위솔은 52% 차광에서 생육이 가장 양호하였으며, 82% 차광까지는 생육이 감소하기는 하지만 상품성은 유지되어 내음성이 뛰어난 것으로 판단되었다. 90% 이상의 차광에서는 고사주가 발생하였으며, 엽색도 탈색되고 잎이 위로 서는 등 관상가치가 크게 저하되었다. 분용토로는 마사토 : 유비상토 : 강모래(6 : 2 : 2, v/v/v) 에서 생육이 가장 좋았다. 특히 지상부 생체중의 경우 다른 용토에서는 약 4~8g 범위였으나, 마사토 : 유비상토 :강모래(6 : 2 : 2, v/v/v)에서는 16g으로 2배 이상의 생육량을 보였다. 시비조건은 Hyponex 액비를 1,000~2,000배액으로 1회/주 처리했을 때, 생체중 및 초폭, 분지수 등에서 가장 좋게 나타났다. 특히 생체중의 경우 대조구(무처리)가 16g인데 비해 1,000배액 1회/주와 2,000배액 1회/주 처리에서 약 29g으로 80% 정도 생장량이 증가하였다. 엽색에서는 액비의 농도가 높을 수록 엽색이 짙어지는 경향이 보였다.

• 유기물자원화
• /
• 제22권4호
• /
• pp.28-44
• /
• 2014

국내 경제성장에 힘입어 최근 30여 년 간에 걸쳐서 축산물 소비가 급격하게 늘어나고 있다. 이에 따라 국내 가축 규모는 전업화, 대형화 되는 추세에 있다. 가축 사육두수의 증가는 필연적으로 가축분뇨 발생량 증가를 초래하게 되었다. 농림축산 통계에 따르면 2013년 말 기준으로 약 47,235천 톤의 가축분뇨가 발생되었다. 이 분뇨를 처리하기 위해서 퇴비화, 액비화, 정화 그리고 혐기소화와 같은 다양한 가축분뇨 처리방법이 적용되고 있다. 가축분뇨를 퇴비화 처리 하는 시설에 있어서는 에스컬레이터식, 패들식, 스크루식 또는 로타리식 등의 4가지 종류의 교반시설이 적용되고 있다. 가축분뇨 액비화 시설의 경우에는 호기적 방법과 무산소적 방법 등의 2가지 시설이 운영되고 있다. 가축분뇨를 액비화하기 위해서 전국에 약 8천기에 달하는 액비화 시설이 설치, 운영되고 있다. 가축분뇨 정화처리 시설에서는 고액분리 처리, 2차 처리 그리고 고도 처리의 3가지 단계의 기술을 적용하고 있다. 2012년 말 기준으로 볼 때 혐기소화 처리방법에 의해서 처리된 돼지분뇨 슬러리는 약 21천 톤에 달한다. 가축분뇨 처리를 위해서 퇴비, 액비, 정화 시설, 혐기소화 시설 등을 선택함에 있어서 악취제거 효율을 높이는 것이 대단히 중요한 사안이 되었다. 본 연구에서는 국내에 설치된 가축분뇨처리 시설의 특징과 운영방식을 6년간에 걸친 시설 설치관련 서류 검토와 현장조사를 통하여 분석하였다.