• Maxillofacial Plastic and Reconstructive Surgery
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• v.18 no.4
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• pp.746-757
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• 1996

Bony defects may be found as a result of congenital anomalies, traumatic injury, automobile collisions and industrial accidents in the maxillofacial area. Such conditions are often associated with severs functional and esthetic problem. Various surgical procedure has been utilized in attempts to repair and reconstruct bony defects. Bone is a complex, living, constantly changing tissue. The architecture and composition of cancellous and cortical bone allow the skeleton to perform its essential mechanical functions. Periosteum covers the external surface of bone and consists of two layers : an outer fibrous layer and an inner more cellular and vascular layer. The inner osteogenic layer or cambium layer can form new bone while the outer layer firms part of the insertions of tendons, ligaments and muscles. This study was under taken to evaluate bone healing process on partial defect of calvarial bone with or without periosteum in rat. We made calvarial defects of different size(4mm, 6mm, 8mm) with periosteum or without periosteum in rat to study the effect of defect size on healing process. Control and experimental groups sacrified at 1, 2, 4, 6, 8 weeks, postoperatively. We examed the specimens by gloss findings, light microscophy, and fluorescent microscophy. The results were as follows. 1. Gloss findings: Control groups are larger bony defects than experimental groups after 2 weeks, and than control groups advanced healing of defected bone but experimental groups are lesser after 4, 6 weeks. After 8 weeks, bone defect has not been identified in control and experimental groups. 2. Light microscope: All defects of control groups are larger bony defects than experimental groups after 2 weeks. And than control groups show smaller defect after 4 weeks. After 8 weeks, the control group reveal pin-point sized, hardly identifiable defect space and the experimental group reveal small, but definite defect space. 3. Fluorescent microscope : Each week, new bone formation of control group is very similar to the experimental group. In this study, Osteogenesis of calvarial bone defects with periosteum or without periosteum was examined for 8 weeks in rats. The replaced periosteum had batter new bone formation than the removed periosteum.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.12
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• pp.57-64
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• 2017

Today, due to the environmental regulations regarding air pollution in the EU, the use of EPS (Styrofoam) as the cushioning material in the packaging industry is decreasing. In effect, air cushioning based cushioning materials are rapidly expanding into the market and replacing EPS, due to their excellent buffering ability and environmental friendliness. This is a new selective filling type air filling material manufacturing technology that affords improvements in the amount of raw materials required, its processing and its aesthetic appearance compared to the conventional air filling cushioning materials. In this study, a multi-stage air cell filling valve molding technology is developed based on selective filling technology, which allows packages to be selectively filled in various forms by applying valve forming structure technology. This multi-stage air cell filling valve molding technology is a technique in which a plurality of injection ports are formed by laminating three layers of films, viz. a first injection film, a valve film, and a second injection film having valve ends. In the conventional technology, a separate external air injection path for injecting air into a plurality of connected air bags is needed. However, in the proposed system, an external air injection path is formed inside the air bag, Due to the lack of need for an injection furnace, the raw material and process are reduced and air is injected and then discharged, while the air bag is reduced in length to 63 ~ 66% of its normal value. The outer surface of the outer air injection path is integrated inside by maintaining the original length of the cross section, while the unnecessary folded air is injected into the interior of the air bag, This smart air filling type cushioning material manufacturing technology constitutes a big improvement over the existing technologies.

• Applied Microscopy
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• v.39 no.1
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• pp.9-15
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• 2009

The ultrastructure of the fertilized egg envelope in Cichlasoma managuensis belonging to Cichlidae were investigated by routine light and electron microscopes. The fertilized eggs of Cichlasoma managuensis was of the light yellow, non-transparent, ellipsoidal, adhesive and nonfloted type. The size of fertilized egg was the major axis $1.92{\pm}0.08\;mm$, the minor axis $1.43{\pm}0.04\;mm$. The egg envelopes have a single micropyle, which is thought to the pathway of sperm in the area of the animal pole. An outer surface of fertilized egg envelope was covered by a adhesive reticular structures and the fertilized egg envelopes consisted of two distinct layers; an outer adhesive layer and an inner layer of $13{\sim}15$ horizontal lower electron density lamellae alternating with interlamellae of higher electron density. The external shape of fertilized egg is common trait of fishes belonging to Cichlidae and these ultrastructural characters of fertilized egg envelope can be utilized in taxonomy of teleost.

• Applied Microscopy
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• v.39 no.3
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• pp.237-243
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• 2009

Chinese minnow, Rhynchocypris oxycephalus is a teleost belonging to Leuciscinae, Cyprinidae. The oogenesis and ultrastructure of egg envelope in Chinese minnow were investigated by light and electron microscopes. The ovary was of white yellowish and ellipsoidal shape with the major axis 30 mm and the minor axis 7mm. Cytoplasm of oogonia was basophilic and many nucleoli were located at inside of nuclear membrane. In primary oocytes, yolk vesicles were distributed only in the marginal area and egg envelope was not formed on the outside of an egg. In secondary oocytes, the egg envelope was formed and yolk vesicles in the cytoplasm were increased than the earlier stage. The basophilic substance of cytoplasm was changed to acidic. In case of matured egg, thickness of egg envelope and size of egg were increased. The yolk vesicles were changed to yolk mass in accordance with development. The outer surface of egg envelope was covered by microvilli-structures, and had a micropyle on the area of animal pole. Egg envelope consisted with 2 layers, an adhesive outer layer with microvilli-structures and fibrillar inner layer. In conclusion, the oogenesis of Chinese minnow was characterized by the increase in cell size, the formation and accumulation of yolk, and the decrease of basophilic substance in the cytoplasm. The oogenesis of Chinese minnow seems to share common patterns in Cyprinidae, but these ultrastructural unique characters of egg envelope can be utilized in taxonomy of teleost.

• Journal of Korean Society of Forest Science
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• v.86 no.3
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• pp.288-300
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• 1997

Morphology and anatomy of the fruit and seed of eight species of Rhus (Anacardiaceae) were investigated. The inflorescence of R. typhina was an apical and erect thysus, that of R. javanica was panicle. while that of the others were an axillary and pendulous panicle. Anatomical investigation of the pericarp showed that exocarp and mesocarp in R. javanica and R. typhina were integrated, but its in the others were disintegrated and uniform. In all these species, endocarp consisted of distinctly three lignified cell layers (outer, middle and inner endocarp) and crystal layers. The surface sculpturing of mature seed was variable and also contributed to understanding of species delimitation and relationships within the genus. Categorization by cluster analysis with 27 characters obtained from morphological and anatomical features of the fruit and seed resulted in two groups : R. trichocarpa - R. javanica group vs. R. ambigua - R. verniciflua - R. sylvestris - R. succedanea group. Taxonomic significance for the genus by these characters was supported by results of principal component analysis. The keys for the genus Rhus were provided using morphological and anatomical characters of the inflorescence, and fruit and seed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.4
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• pp.415-430
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• 1996

Spatio-temporal variations in zooplankton community and ropepod indicator species were investigated along with the interaction between zooplankton distribution and environmental factors in Chinhae Bay. Zooplankton samples were monthly collected at 7 stations from February to September in 1993. A NORPAC net was vertically hauled from bottom to surface, At the same station, environmental factors such as temperature, salinity and COD (chemical oxygen demand) were measured at two different water layers, surface and bottom. In August and September, salinity declined below 30.00‰ , while eutrophic parameters such as COD showed the higher concentrations than those in other months, with higher concentrations at inner bay stations. Salinities were, however, higher at bay mouth areas. These distributional patterns were believed to be caused by input and dispersion of organic matters from nearby land. Zooplankton communities were composed of 7~14. Of these, Noctiluca scintillans was predominant and occupied 90.6‰ of total zooplankton abundance. Cladocera and Copepoda were secondly abundant taxa. Among 6 to 10 copepod species appeared, Acartia omorii and A. hudsonics were most common species during the survey months except March and September. Species diversities were greater, in general, at inner bay than outer bay. The lowest diversity index was observed in February, while the highest in July. Cluster analysis could divide the study area into 2 or 4 zones for each month. Zone 1, mouth area of the bay, was characterized by the influence of offshore waters. Zone II was mixing area. Zone III and IV seemed to be affected by nearby land.

• Journal of Sericultural and Entomological Science
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• v.24 no.2
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• pp.55-72
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• 1983

These studies were done to find out any difference, ultrastructural, physical or chemical, between the shells of diapausing and non-diapausing eggs of the silkworm, Bombyx mori L. 1. From the electron-microscopic observation, the egg shells have four distinctive layers. In addition to the four layers, the shells in the diapausing eggs has another layer with low electron density on its surface. 2. The permeability of the egg shell to hydrochloride was much lower in diapausing egg than in non-diapausing egg. Also the permeability changed in the opposite directions with the egg age: the diapausing eggs decreased while non-diapausing ones increased. 3. The permeability increased when the diapausing egg shell was treated with HCl. When they were treated with ether, however, the increase in permeability was much smaller. It seems there was an ether soluble material involved in the content of the egg shell. 4. The diapausing eggs were also much more resistant to desiccation than the non-diapausing ones. The former, when treated with HCl or chilling, became less resistant to desiccation. 5. The positive histochemical response of the egg shell to PAS-Alcian blue and protein stainings suggests presence of abundant proteins and carbohydrates in the egg shell. On the other hand, the staining response to lipid was more positive in the inner layers than in the outer layer of the shell. 6. The egg shell adhesives seems to be mucopolysaccharides produced by colleterial glands, since the oviposited eggs showed a positive responses to carbohydrate and negative to lipid-staining chemicals, but not the mature oocytes in the ovarioles. 7. There were two bands on the electrophoretic pattern of the SH proteins extracted from the egg shells both in the diapausing egg and non-diapausing one: a slow moving major component and a fast moving minor one. However, the electrophoretic mobility showed a difference in the minor components between them. It is evident that the fast moving minor one of non-diapausing egg ran a little further than that of diapausing egg. 8. In amino acids analysis, no significant differences were found in their composition between diapausing and non-diapausing egg and SH proteins contain relatively more glycine and less cystine.

• Horticultural Science & Technology
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• v.32 no.2
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• pp.171-177
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• 2014

In an effort to investigate special distribution of glucose content of potato tuber of a cultivar 'Superior' after harvest, a whole tuber was longitudinally cut into halves and cut-surface was divided into 11 regions. Approximate glucose concentration of each section was determined using a commercial blood glucose meter. Higher level of glucose was detected in the outer layers of tissue than inner part of tuber although there were lower coefficients of variation value, 37.4% and 34.1% among individual tubers and among defined sections, respectively. A positive correlation between the whole tuber and individual section was existed in glucose content, where the central pith tissue gave the highest coefficient (r = 0.921) and bud end tissue did the lowest (r = 0.544). Glucose content of the tubers stored for 4 months at $2.0^{\circ}C$ was 5.5 fold higher compared to the tubers kept in ambient temperature. The chip color of the former tubers was much brighter than those of the latter tubers. The result obtained in the present study suggests that the blood glucose meter can be used to a rapid and simple evaluation of glucose content and therein be applied to estimate the processing quality of potato tubers during postharvest handling.

• Development and Reproduction
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• v.2 no.1
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• pp.75-80
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• 1998

메기 정자는 그 길이가 약 62.5 \mu m이며 구형의 핵, 짧은 중편 및 꼬리를 ㄱ진 일반적인 메기류 정자의 미세구조적 특징을 나타내었다. 정자는 대부분의 경골어류의 정자에서와 같이 첨체를 가지고 있지 않았으며 염색질은 완전치 농축되어 있었다. 핵와(nuclear fossa)는 약 0.6 \mu m 함입되어 있었고 그 속에 기부 중심립과 말단 중심립의 일부가 포함되어 있었다. 두 중심립은 140 \circ C의 각도로 배열되어 있었으며 말단 중심립에서 9개의 부수체가 언형질막을 향하여 배열되어 있었다. 미토콘드리아는 중편 세포질에서 2층 또는 3층으로 배열되어 있었으며 핵의 후반부와 꼬리의 기부를 둘러싸고 있었다. 꼬리는 축사만으로 구성되어 있었으며 lateral fins는 관찰되지 않았다. 메기 정자의 가장 큰 구조적 특징은 중편 세포질에 구성되어 있는 관구조(tubular structure)이었다. 대부분의 경골어류의 정자는 중편 세포질에 미토콘드리아만을 포함하고 있으나, 메기 정자에서는 중편 세포질의 전반부에 미토콘드리아가 포함되어 있고, 후반부에는 소관이 모여 망상구조를 형성하는 관구조가 잘 발달되어 있었다. 이와 같은 관구조는 현재까지 Characiformes의 정자 이외의 다른 경골어류에서는 보고된 바 없으며 이러한 구조는Characiformes과 메기류의 계통학적 관계를 연구하는데 매우 중요한 형질로 여겨진다. ^u The spermatozoa of Silurus asotus are appoximately 62.5 \mu m in length and relatively simple cells composed of spherical head, a short midpiece and a tail as in most Siluriformes. The ultrastructure of the spermatozoa of S. asotus is characterized by the following features. The nucleus measuring about 1.5 \mu m in length is depressed with a deep nuclear fossa of about 0.6 \mu m in length, two fifth of the nuclear diameter. The fossa contains the proximal centriole and the half of the distal centriole. Two centrioles form an angle of approximately 140 \circ to each other. the nine satellite rays radiate from the outer surface of the distal centriole. the mitochondrea surround the basal nucleus and the axoneme, and are arranged in two or three layers in the postnuclear cytoplasm. The lateral fins are lost in the sperm tail. The most significant feature is manifested in the midpiece. The midpece comprises two parts, the mitochondria and the tubular structure unlike other teleost fishes containing only the mitochondria. The tubular structure was reported only in the spermatozoa of Citharinus belonging to the characiformes of teleost fishes. Thus it is considered to be a good characteristics for the study of phylogenetic link between Siluriformes and Characiformes.

• Elastomers and Composites
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• v.39 no.2
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• pp.131-141
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• 2004

The recycling of waste XLPE(crosslinked polyethylene), which is a major source of scraps from high voltage power transmission cables, has been discussed. The waste XLPE scraps were ground into fine powder with various sizes from less than $100{\mu}m$ up to about $1000{\mu}m$ using two types of tailor-made pulverizers. The compounds were prepared in a modular intermeshing co-rotating twin screw extruder at various conditions such as different compositions, types and powder sizes of waste XLPE, screw configurations and various polymer matrices (LDPE, HDPE, PP, PS). The mechanical and rheological properties and the fracture surface or the compounds were investigated. It was found that an improved impact strength was obtained from the compound with white XLPE powder pulverized from the scraps without outer/inner semi-conductive layers. Generally, the impact strength increases with the content of XLPE but decreases with the size of XLPE. Especially for LDPE, the extrusion was possible up to 80 wt% loading of XLPE. Also, the impact strength increases with the number of kneading disc blocks in the given screw configurations. The melt viscosity of the compounds increases with increasing XLPE loading. However, the higher shear thinning behavior of the compounds at common shear rates implies proper processibility of the compounds. In addition, the impact strength for other polymer matrices used increases with XLPE and it is noticeable that the impact strength of PS/XLPE (80/20 wt%) compound was improved twice that of pure PS.