• 한국임상수의학회지
• /
• 제28권4호
• /
• pp.449-451
• /
• 2011

1.28 kg, 수컷, 13살의 붉은귀 거북이(red-eared slider, Trachemys scripta elegans)가 한 달 간의 식욕 부진, 운동성 감소, 전지 부종 및 발적을 주증으로 내원하였다. 고요산혈증과 함께 방사선 앞발가락과 손목 관절의 비투과성 을 동반한 골융해 소견을 보이는 토피성 통풍(tophaceous gout)이 방사선 검사 상에서 확인되었다. Allopurinol (20 mg/kg, 경구, 하루 한 번)과 u/d (Hill's diet)를 처방하였으며 다른 소염제나 항생제는 투여하지 않았다. 내원 한달 후, 거북이의 임상 증상과 방사선 소견은 호전된 양상을 보여주었다. 약물 반응에 따라, 거북이는 관절 통풍으로 추정 진단되었으며 allopurinol은 거북이의 관절 통풍에 효과적임을 보여주었다.

• 대한골관절종양학회지
• /
• 제19권2호
• /
• pp.78-82
• /
• 2013

랑게르한스 세포 조직구증(Langerhans' cell histiocytosis)은 단핵구계에 속하는 정상 조직구들이 과도하게 증식하여 다양한 임상 경과와 치료결과를 보이는 질환 군으로 알려져 있다. 특히, 척추에 침범하는 경우에는 단발 혹은 다발성의 골 용해를 특징으로 한다. 병변의 침범 부위, 진행 정도 및 동반 증상에 따라 단순 경과 관찰에서부터 방사선 치료, 화학요법 및 수술적 치료에 이르기까지 다양한 치료법들이 적용되어 왔으나, 아직까지 확립된 치료법은 없는 것으로 알려져 있다. 저자들은 경추와 요추를 동시에 침범하면서 척추 내 골수 파괴 소견과 병적 골절을 동반한 단일조직 랑게르한스 세포 조직구증 환자에 대해 수술적 치료 및 전신화학요법을 시행한 1예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다.

• Journal of Korean Neurosurgical Society
• /
• 제51권3호
• /
• pp.151-154
• /
• 2012

We report here two cases of primary intraosseous meningioma with aggressive behavior. A 68-year-old man presented with a one year history of a soft, enlarging mass in the right parietal region. Magnetic resonance image (MRI) revealed a 6 cm sized, heterogeneously-enhancing, bony expansi1e mass in the right parietal bone, and computed tomograph (CT) showed a bony, destructive lesion. The tumor, including the surrounding normal bone, was totally resected. Dural invasion was not apparent Diagnosis was atypical meningioma, which extensively metastasized within the skull one year later. A 74-year-old woman presented with a 5-month history of a soft mass on the left frontal area. MRI revealed a 4 cm sized, multilobulated, strongly-enhancing lesion on the left frontal bone, and CT showed a destructive lesion. The mass was adhered tightly to the scalp and dura mater. The lesion was totally removed. Biopsy showed a papillary meningioma. The patient refused adjuvant radiation therapy and later underwent two reoperations for recurred lesions, at 19 and at 45 months postoperative. The patient experienced back pain 5 years later, and MRI showed an osteolytic lesion on the 11th thoracic vertebra. After her operation, a metastatic papillary meningioma was diagnosed. These osteolytic intraosseous meningiomas had atypical/malignant pathologies, which metastasized to whole skull and the spine.

• Archives of Pharmacal Research
• /
• 제26권11호
• /
• pp.929-936
• /
• 2003

Methanol extract (MeOH), n-hexane (Hx), chloroform ($CHCl_3$), ethyl acetate (EA), butanol (BuOH) and aqueous ($H_2O$) fractions of Eucommiae Cortex including geniposidic acid (GA), geniposide (GP) and aucubin (AU) were tested for their therapeutic efficacy on osteoporosis. The contents of GA, GP and AU in the cortex and leaf of Eucommia ulmoides Oliver were quantified by HPLC. The effect of Eucommiae Cortex on the induction of growth hormone (GH) release was studied by using rat pituitary cells. The proliferation of osteoblast-like cells increased by herbal extracts was assayed using a tetrazolium (MTT), alkaline phosphatase (ALP) activity, and [$^3H$]-proline incorporation assays. The inhibition of osteoclast was studied by using the coculture of mouse bone marrow cells and ST-2 cells. As a result, the GA, GP and AU were present in the cortex more than in the leaf of E. ulmoides Oliver. The MeOH (1mg/mL), Hx, $CHCl_3$ and EA fractions (each 20 $\mu$ g/mL) had potent induction of GH release. The $CHCl_3$ exhibited the potent proliferation of osteoblasts. The AU, GP and GA were increased proliferation of osteoblasts. In addition, GA ($IC_{50}: 4.43{\times}10^{-7}$M), AU and GP were significantly inhibited proliferation of osteoclast. In summary, it is thought that the components in a part of the fractions of Eucommiae Cortex participate in each step of mechanism for activating osteoblast to facilitate osteogenesis, and suppress osteoclast activity to inhibit osteolysis.

• 한국방사선학회논문지
• /
• 제12권1호
• /
• pp.107-114
• /
• 2018

고관절 시행에서 식립각도는 추후 환자의 운동범위나 힘의 분배조건에 따라 폴리에틸렌 라이너의 마모에도 영향을 미친다. 인공관절요소로부터 마모입자는 골괴사나 다른 생화학적으로 많은 합병증을 일으킨다. 이런 점에서 인공고관절에서 마모와 정렬각도를 수술 후 추시를 통해 측정하는 것은 중요하다. 특히 관절면의 마모를 측정하는 것은 그 량이 크지 않아 상당한 정밀도를 요구한다. 현재 널리 쓰이는 상용 소프트웨어는 재현성과 측정방법의 표준화가 되어 있지 않아 사용에 많은 어려움이 있다. 본 논문에서는 일반 병원의 방사선촬영 환경에서 CAD만 추가로 구비하면 행할 수 있는 인공관절면 마모와 식립각 측정법을 제시한다. 인공관절만의 X-ray영상을 이용한 본 방법의 정확도와 정밀도 평가를 수행하였다. 또한 실제 인공고관절환자의 X-ray영상을 가지고 2년추시에 따른 마모와 식립각을 측정하였다. 본 연구에서 제시한 CAD를 이용한 방법은 마모측정에서 정확도 0.06 mm, 정밀도 0.05 mm, 식립각은 $0.27^{\circ}$의 정밀도를 갖는다.

• 한국임상수의학회지
• /
• 제25권2호
• /
• pp.126-130
• /
• 2008

6연령의 말티즈 견이 오른쪽 견갑부의 종괴로 내원하였다. 신체검사와 자기공명영상(MRI)의 평가, 전체혈구 계산 (CBC), 혈청화학검사, 미세흡인 생검(FNA), 조직학적인 평가가 이루어졌다. 전체혈구계산 검사에서는 정상범위를 나타내었으며, 혈청화학 검사상에서는 ALP의 증가를 보였다. 방사선 소견에서 견갑골의 완전한 골 융해 소견을 보였으며, 자기공명영상에서는 혼재된 신호강도를 보였다. 면역조직화학(염색과 Masson's trichrome 염색) 소견에 근거하여, 섬유육종으로 최종진단 되었다.

• 대한골관절종양학회지
• /
• 제8권3호
• /
• pp.90-95
• /
• 2002

42세 남자 환자가 우측 족관절부에 4개월 동안 증가하는 동통 및 종괴를 주소로 내원하였다. 단순 방사선 소견 및 전산화 단층 촬영 소견상 우측 거골 경부의 골내부 및 피질골 파괴소견을 보였다. 병리소견은 고악성도의 골형성 골육종이었다. 두 사이클의 수술전 항암치료에도 불구하고, 족관절부의 종괴는 커졌고 폐전이로 진행되었다. 원발 병소에 대하여 슬관절하 절단, 폐전이에 대하여 쐐기모양 폐 절제술을 실시하였고 수술후 항암치료를 시행하였으나 수술후 10개월에 폐전이로 환자는 사망하였다.

• 한국임상수의학회지
• /
• 제33권5호
• /
• pp.300-303
• /
• 2016

An age-unknown, 4.8 kg, male, wild, domestic short-hair cat was presented for left hindlimb lameness. A physical examination revealed a draining tract which was suspected of bite on left calcaneal bone. The left tarsal joint was markedly swollen and exudates were observed around the draining tract. Sequestrum at left calcaneus bone, and osteolysis were identified by radiography. The sequestrum and its surrounding exudative tissue were debrided during surgery and the tissue was submitted for bacterial culture and sensitivity test. The debridement caused a bone defect ($1.5cm{\times}0.5cm{\times}0.5cm$) on the medial left calcaneal bone. Plate and screw fixation was performed to the calcaneus bone as buttress plate. Recombinant human bone morphogenetic protein-2 (rhBMP-2) loaded hydroxyapatite was implanted in the bone defect. Furthermore, Amikacin-impregnated collagen sponges were also placed around bone plate to deliver local antibiotics. A systemic antibiotic treatment regimen based on bacterial culture and sensitivity test results was administered for 4 weeks. The wound properly healed without any signs of infection, and the bone healing was confirmed by radiography. The patient showed normal weight bearing ambulation at 18 weeks after surgery. The use of rhBMP-2 and local antibiotic delivery system is a good surgical option for the one-stage treatment of chronic osteomyelitis.

• Journal of Microbiology and Biotechnology
• /
• 제32권2호
• /
• pp.141-148
• /
• 2022

Many bone diseases such as osteolysis, osteomyelitis, and septic arthritis are caused by gram-negative bacterial infection, and lipopolysaccharide (LPS), a bacterial product, plays an essential role in this process. Drugs that inhibit LPS-induced osteoclastogenesis are urgently needed to prevent bone destruction in infective bone diseases. Marein, a major bioactive compound of Coreopsis tinctoria, possesses anti-oxidative, anti-inflammatory, anti-hypertensive, anti-hyperlipidemic, and anti-diabetic effects. In this study, we measured the effect of marein on RAW264.7 cells by CCK-8 assay and used TRAP staining to determine osteoclastogenesis. The levels of osteoclast-related genes and NF-κB-related proteins were then analyzed by western blot, and the levels of pro-inflammatory cytokines were quantified by ELISA. Our results showed that marein inhibited LPS-induced osteoclast formation by osteoclast precursor RAW264.7 cells. The effect of marein was related to its inhibitory function on expressions of pro-inflammatory cytokines and osteoclast-related genes containing RANK, TRAF6, MMP-9, CK, and CAII. Additionally, marein leads to markedly inhibited NF-κB signaling pathway activation in LPS-induced RAW264.7 cells. Concurrently, when the NF-κB signaling pathway was inhibited, osteoclast formation and pro-inflammatory cytokine expression were decreased. Collectively, marein could inhibit LPS-induced osteoclast formation in RAW264.7 cells via regulating the NF-κB signaling pathway. Our data demonstrate that marein might be a potential drug for bacteria-induced bone destruction disease. Our findings provide new insights into LPS-induced bone disease.

• 한국임상수의학회지
• /
• 제39권3호
• /
• pp.131-137
• /
• 2022

Osteomyelitis typically occurs because of the direct inoculation of bacteria or fungi after penetrating trauma or surgical contamination or, by extension, from soft tissue infection. Osteomyelitis is rarely reported in wildlife animals, though severe chronic osteomyelitis cases do exist in wildlife owing to the scarcity of medical support in the wild environment. This report describes three cases of chronic osteomyelitis in wild raccoon dogs related to trauma. The typical symptoms of three reported cases were ataxia, stiffness, muscle atrophy, and lethargy. All three cases were relevant to traumatic or severe external injury, and skin infestation caused by ectoparasites was apparent on an ocular inspection. In the radiographic examination, diffuse sites of osteolytic lesions and remarkable periosteal responses were demonstrated around the injured limb in all three cases. Apparent neutrophilia with a left shift, lymphocytosis, and monocytosis in hematological examinations generally indicated chronic infection as shown in case 1 and 3. Treatment was attempted with broad-spectrum antibiotics and non-steroidal anti-inflammatory drugs, such as amoxicillin/clavulanic acid, enrofloxacin, clindamycin, and meloxicam. These treatment options helped improve the overall prognosis of chronic osteomyelitis, but the outcomes did not meet the treatment goal entirely. Osteomyelitis can be extremely challenging to treat, particularly in wild animals, because of their distinctive traits, such as masking phenomenon and uncontrolled exposure to ectoparasites. Earlier diagnosis with a radiographic examination, hematological examinations, and careful patient monitoring, followed by prolonged antibiotic therapy and restricted exercise, are the key factors leading to a better prognosis.