• International Journal of Oral Biology
• /
• v.44 no.4
• /
• pp.165-172
• /
• 2019

Berberine has been used clinically for more than a decade to treat various diseases, has been shown to be effective in osteoblast differentiation, and is a potential treatment option for osteoporosis. However, compared with existing osteoporosis drugs, berberine is somewhat less effective. This study aimed to identify a new compound with efficacy superior to that of berberine. The osteogenic activities of various berberine derivatives were evaluated via cell differentiation in C2C12 preosteoblast cell lines. Alkaline phosphatase (ALP) staining assay and structure-activity relationship demonstrated that compound 2b had a potent osteogenic effect. Furthermore, compound 2b dose dependently increased ALP activity and showed no toxicity at the effective concentration, indicating its efficacy. Additionally, compound 2b upregulated BMP2-induced transcriptional activity in a promoter activity assay using ALP, BSP, and OC promoters.

• The Journal of the Korea Contents Association
• /
• v.9 no.11
• /
• pp.247-253
• /
• 2009

We investigated the cell growth rate according to the change of temperature of the Thermo-rod used for the local hyperthermia therapy. For this study, we fabricated the Thermo-rods (TR) using Duplex Stainless Steels having magnetic properties as well as non magnetic properties. To evaluate cell growth rates up to 15 days, we conducted cell proliferation test using cell counting methods. For the tests, the CAPEs and Osteoblats were seeded on the 6-we11 plates with the induction heated thermo-rods 30 mins a day for 15 days with 2 days interval and without induction heated thermo-rods as control group respectively. We calculated cell growth rates, 6 hours after heating. From the results, in case of CAPEs and Osteobalsts seeded groups, the cell growth rates in all groups increased drastically for 6 days after seeding, but decreased irregularly after 6 days. In conclusion, the cell growth rates showed no significant difference among all groups and it indicated that there were no effects of temperate ($41^{\circ}C$) on cell growth rates.