• 환경생물
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• 제41권4호
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• pp.364-369
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• 2023

Two newly recorded sea stars, Henricia densispina (Sladen, 1879) and H. reniossa asiatica Djakonov, 1958, collected from the East Sea of Korea using fishing nets, were described in this study. Morphologically, H. densispina is characterized by the shape (sub-triangular) similarity of the intermarginal plates to the superomarginal plates. Henricia reniossa asiatica Djakonov, 1958, has long and slender arms (R/r=7.2-9.1); clustered abactinal paxillae with 3-13 abactinal spinelets; a denuded abactinal skeleton made up of crescentic, elongated cross-shaped, the presence of small ossicles that divided the papular areas; and an adambulacral armature comprised of 14-17 spinelets. This study provides the first occurrence of H. densispina and H. reniossa asiatica in the Korean fauna and a detailed morphological description of these species' key characteristics is provided.

• Applied Microscopy
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• 제46권2호
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• pp.93-99
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• 2016

The thickness of specimen is an important factor in microscopic researches. Thicker specimen contains more information, but it is difficult to obtain well focused image with precise details due to optical limit of conventional microscope. Recently, a microscope unit that combines improved illumination system, which allows real time three-dimensional (3D) image and automatic z-stack merging software. In this research, we evaluated the usefulness of this unit in observing thick samples; Golgi stained nervous tissue and ground prepared bone, tooth, and non-transparent small sample; zebra fish teeth. Well focused image in thick samples was obtained by processing z-stack images with Panfocal software. A clear feature of neuronal dendrite branching pattern could be taken. 3D features were clearly observed by oblique illumination. Furthermore, 3D array and shape of zebra fish teeth was clearly distinguished. A novel combination of two channel oblique illumination and z-stack imaging process increased depth of field and optimized contrast, which has a potential to be further applied in the field of neuroscience, hard tissue biology, and analysis of small organic structures such as ear ossicles and zebra fish teeth.

• 대한디지털의료영상학회논문지
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• 제17권1호
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• pp.49-56
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• 2015

Temporal bone CT scan side skull fracture. In addition to the confirmation of the ossicles, such as fractures and dislocations, temporomandibular facial fractures, deformities surgery helps to establish a science plan. Cochlear implant surgery has been performed in the state before and after identifying purposes. Test methods are being implemented by the Conventional direct axial and Direct coronal scan, the basic method of Temporal bone CT. Helical scan is a fast Volumetric data obtained compared with the Conventional scan, the patient reduced the dose, and there are some advantages, such as reduced Beam hardening streak artifacts caused by dental fillings. This study is a comparative analysis by dose reduction for patients with a dose according to the conventional scan method and then effective from 2015 by helical scan method performed in 2014 through the retrospective survey, which was then optimized for the purpose of inspection.

• Journal of Yeungnam Medical Science
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• 제8권1호
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• pp.127-135
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• 1991

성공적인 종이수술을 위하여 안전하고 효과적인 접착제가 필요하며, 합성 접착제의 독성작용 때문에 인체에서 추출한 접착제를 사용하게 되었다. Fibrin 접착제가 저장 혈액에서 체취되면 감염성 질환의 전염 위험성이 있지만 자가 fibrin 접착제를 사용하면 전염위험성이 없다. 저자들은 ammonium sulfate fibrin 접착제와 상품화된 fibrin 접착제의 접착력을 비교한 결과 ammonium sulfate fibrin 접착제의 접착력이 상품화된 fibrin 접착제의 반 정도의 접착력을 가지지만 그 정도의 접착력이면 고막재생술, 안면신경 봉합술, 이소골 재건술, 외이도 후벽재건술, 뼈가루를 이용한 전두동 및 유양동폐쇄술 등 이비인후과 수술에 사용하기엔 충분한 접착력이다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제35권5호
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• pp.304-309
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• 2009

Purpose: Fibrous dysplasia (FD) is a fibro-osseous disease associated with activating missense mutations of the gene encoding the $\alpha$-subunit of stimulatory G protein. FD may affect a single bone (called monostotic form) or multiple bones (called polyostotic form). The extent of lesions reflects the onset time of mutation. In this study, cells from monostotic FD in maxilla of a patient were isolated and cultured in vitro for characterization. Materials and Methods: The single cells were released from FD lesion which was surgical specimen from 15 years-old boy. These isolated cells were cultured in vitro and tested their proliferation activity with MTT assay. In osteogenic media, these cells underwent differentiation process comparing with its normal counterpart i.e. bone marrow stromal cells. The proliferated FD cells were detached and transplanted into the dordsal pocket of nude mouse and harvested in 6 weeks and 12 weeks. Results and Summary: FD cells have an increased proliferation rate and poor differentiation. As a result, cells isolated from FD lesion decreased differentiation into osteoblast and increased proliferation capacity. MTT assay presented that proliferation rate of FD cells were higher than control. However, the mineral induction capacity of FD was lesser than that of control. Monostotic FD cells make fewer amounts of bone ossicles and most of them are woven bone rather than lamellar bone in vivo transplantation. In transplanted FD cells, hematopoietic marrow were not seen in the marrow space and filled with the organized fibrous tissue. Therefore, they were recapitulated to the original histological features of FD lesion. Collectively, these results indicated that the FD cells were shown that the increased proliferation and decreased differentiation potential. These in vitro and in vivo system can be useful to test FD cell's fate and possible.

• 한국어류학회지
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• 제30권3호
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• pp.137-143
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• 2018

이 연구의 목적은 우리나라 연안에 서식하는 두줄망둑의 자치어 골격발달을 관찰하여 분류학적 연구자료로 이용하는 것이다. 부화 후 7일째 평균전장 $4.44{\pm}0.28mm$ (n=5) 일 때 섭이기능을 하는 윗턱에는 전상악골, 주상악골이 골화하였고, 아래턱에는 악골이 골화하기 시작하였다. 부화 후 13일째 평균전장 $5.62{\pm}0.24mm$ (n=5) 일 때 두개골의 액골, 노정골, 상이골이 골화하였다. 부화 후 32일째 평균전장 $11.8{\pm}0.28mm$ (n=5) 일 때는 견대부에 사출골, 후쇄골이 골화하였고, 부화 후 40일째 평균전장 $13.3{\pm}0.21mm$ (n=5) 일 때 두개골의 측사골이 골화하면서 모든 골격의 골화가 완료되었다.

• 대한족부족관절학회지
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• 제7권2호
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• pp.218-222
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• 2003

Purpose: The objective of this study was to define the Os submalleolare as an any ossicles below medial malleolus of tibia and to determine the characteristics of clinical and radiological characteristics and to evaluate results of surgical treatment of Os submalleolare in sports player group. Materials and Methods: Twenty-two patients with Os submalleolare were identified between November, 1, 1998 and June, 30, 2002. Results: The mean age was 18.3 years. All patient were male and soccer players comprised 71%(20 cases). Associated disease were 8 cases(29%) of chronic ankle instabilities, one case(4%) of anterior impingement syndrome and one (4%) of plantar fascitis. The most common clinical symptom was pain during walking and sports activity and sign was tenderness around medial malleolus. Only simple radiograph could reveal presence of ossicle and differentiate with acute fracture. If symptom and sign obscured, Bone scan(7 cases) and MRI(3 cases) identify causes of pain and tenderness. As a surgical treatment, all bony fragment caused symptom and sign were eliminated and medial collateral ligament was reattached meticulously. Associated chronic ankle instability were present, modified Brostrom procedure was done simultaneously. On follow-up, The symptomatic pain were wholly disappeared at average 2.2 months (1-6months) after operation. On one year follow-up, all patients have been daily life without any complaints and have gone back to the game within three months. Conclusion: as submalleolare have relatively rare incidence in sports players and most common clinical symptom and sign is tenderness on medial malleolar area of ankle, can be diagnosed both physical examination and plain X-ray film. As a treatment, Removal of ossicle and reattachment of MCL could obtain excellent or good results.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제38권1호
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• pp.29-37
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• 2012

Introduction: Fibrous-osseous lesions of the jaws are difficult to diagnose precisely until excised biopsy results are found, so they might be confused with malignant lesions. This clinical study focused on the diagnostic aids of lesions that demonstrate different clinical, radiologic, and histological findings. Materials and Methods: A total of 16 patients with benign fibrous-osseous lesions on the jaws (6 fibrous dysplasias, 6 ossifying fibromas, 3 cemental dysplasias, and one osteoblastoma) were reviewed. Nine patients with malignant fibrous-osseous lesions (8 osteosarcomas and one Ewing's sarcoma) were also retrospectively reviewed. Results: Osteosarcoma patients complained of facial swelling and tooth mobility. The radiographic findings showed the irregular resorption of cortical bone and periosteal reactions. Histological features included cellular pleomorphism and atypical mitosis. An Ewing's sarcoma patient complained of tooth mobility and facial swelling. Onion-skin appearance and irregular expansile marginal bony radiolucency were seen in the radiography. Fibrous dysplasia patients complained of facial swelling and asymmetry. The radiographic features were mostly ground-glass radiopacity. Histological findings showed a bony trabeculae pattern surrounded by fibrous ground substances. Ossifying fibroma patients complained of buccal swelling and jaw pains, showing expanded cortical radiolucent lesions with a radiopaque margin. Histological findings were revealed as cellular fibrous stroma with immature woven bones. In cemental dysplasia, most of their lesions were found in a routine dental exam. Well-circumscribed radiopaque lesions were observed in the radiography, and cementum-like ossicles with fibrous stroma were seen in the microscopy. An osteoblastoma patient complained of jaw pain and facial swelling. Radiographic findings were mottled, dense radiopacity with osteolytic margin. Trabeculae of the osteoid with a vascular network and numerous osteoblasts with woven bone were predominantly found in the microscopy. Conclusion: Our study showed similar results as other studies. We suggest the clinical parameters of diagnosis and treatment for malignant and benign fibrous-osseous lesions of the jaws.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제27권3호
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• pp.248-258
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• 2005

The challenging task of classifying the fibro-osseous(FO) lesions has been previously attempted but only in the past 15 years has the entire spectrum of diversity been appreciated. For the clinicians, it is hard to clearly diagnose the lesions before operations. The purpose of this study was to review the literature about fibro-osseous lesions of the jaws and to analyse our clinical cases. As the results of the review of clinical features, radiography and histopathologic findings of sixteen cases of fibro-osseous lesions, we could elucidate diagnostic aids for treatment of benign FO lesion in jaws. Six patients involving fibrous dysplasia complained the facial swelling and facial asymmetry. The radiographic features of the lesions showed ground-glass radiopacity mostly and the histologic findings showed typically Chinese character-shaped trabeculae without osteoblastic rimming in the fibrous stroma. Six patients with ossifying fibroma were notified as swollen buccal cheek state. Their radiographic findings showed cortical expanded radiolucent lesion with sclerotic defined border, which was contrast to the normal adjacent bone. The lesions showed variant radiolucent lesions. Histological findings were revealed as cellular fibrous stroma with woven bones, variable patterns of calcifications. Three patients with cemental dysplasia didn't have specific complaints. Well circumscribed radiopaque lesions on mandibular molar area were observed. Cementum-like ossicles with fibrous stroma were found on microscopic findings. A osteoblastoma case with jaw pain was found. The radiographic feature was a mottled, dense radiopacity with osteolytic border on mandibular molar area. Under microscopy trabecule of osteoid with vascular network were predominantly found. Numerous osteoblast cells with woven bone were found. These clinical, radiographic and microscopic findings of benign fibrous-osseous lesions would suggest diagnostic criteria for each entity of FO lesions.

• 대한기관식도과학회:학술대회논문집
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• 대한기관식도과학회 1979년도 제13차 학술대회 연제순서 및 초록
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• pp.4.4-5
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• 1979

만성중이염은 이비인후과 영역에 있어서 가장 빈도가 높은 질병중의 하나이며 항생물질 및 화학요법 등 많은 의학의 발달이 있음에도 불구하고 본 질환의 근본적인 치료법으로는 수술요법 밖에 없는 실정이다. 만성중이염의 수술요법은 중이화농의 제거를 위한 근치수술에서 부터 시작되어 근년에 이르러서는 중이염의 제거는 물론 청력개선을 도모하는 소위 고실성형술이 발달하였다. 고실성형술의 술식에 따른 수술후 청력 개선에 관해서는 학자들의 많은 연구가 있으나 술전 청력과 고실내 상태와의 관계는 언급이 적다. 저자들은 술전 청력상으로 중이내 병변의 종류와 정도를 추정할 수 없을가 하는 문제와 전음기구의 기초적, 실험적 이론을 어떻게 적응시키고 응용해야 할 것인가 하는 문제에 착안하여 술전 청력이 가지 는 의의를 규명하고 이에 따라 수술 술식을 선택할 계획의 일부로서, 고실내 병변과 청력과의 관련성을 검토하였다. 연구대상은 1977년 1월 1일부터 1978년 12월 31일까지 2년간 수술현미경하에 고실성형수술을 시행한 만성중이염 189례를 대상으로 술전 청력장애와 술중 관찰한 고실내 병변과의 상관관계를 검토하였다. 관찰항목출 고실내 이소골의 병변을 중심으로 이소골에 특별한 병변없이 고막천공만 있는 군(Ⅰ군), 이소골연쇄의 운동성 및 연속성에 이상이 없이 이소골 주위에만 병적 육아조직이 있는 군(Ⅱ군), 이소골의 연속성은 정상이나 가동성에 이상이 있는 군(Ⅲ군) 및 이소골이단군(Ⅳ군) 마다의 1) 이소골 병변, 고막청공의 크기 및 청력형과 기도청력장애와의 관계, 2) 저음역 골도연장, 고음역 골도장애 및 Carhart's notch와 고실내 병변과의 관계 3) 교실내 육아조직의 발생부위 등과 기도청력과의 관계를 검토하여 그 성적을 다음과 같이 요약한다. 1) 만성중이염 총 189례의 평균 청력손실 역치는 44.6dB였고 기도청력은 이소골 연쇄상태와 관계가 있으며 정상, 육아조직형성, 이소골 운동장애 및 이소골 연쇄의 이단 순서로 청력이 악화하였다. 2) 이소골파괴례의 평균 청력손실 역치는 49.1 dB이며 3개 이소골의 전결손례와 1∼2개의 부분결손례에는 청력손실에 유의한 차이가 있었으나 이소골 부분파괴례 간에는 차이가 없었다. 이소골연쇄에 병변이 없이 고막천공만이 있는 예의 기도청력은 약 45dB이내이며 천공이 커질수록 청력장애가 악화하는 경향이나 고실내 병변에 따라 차이가 있었다. 이소골에 병변이 없는 소천공의 청 력손실은 30dB이네 이며 30dB를 넘는 것은 이소골 기타에 병변이 있었다. 5) 청력형은 수평형과 저음역장애형이 각각 57례(30.2%), 67례(35.4)로 가장 많았으며 고음역장애형은 이소골의 운동장애가 있는 예에 많은 경항이었다. 6)이소골의 가동성이 유지되면서 어떤 형태의 부하가 있을 때는 저음역골도의 연장이 있고 고음역골도의 악화는 중이염으로 인한 내이병변으로 온다고 추정한다. 7) 소위 Carhart's notch는 14례(7.4%)했으며 이소골연쇄의 강직례에서 발생한다고 생각된다. 8)고실내 병리조직학적 염증형과 청력장애와는 관계가 없었다. 9)고실내 육아조직의 발생부위 특히 전정창과 와우창상의 병변은 청력에 영향을 주었다. 10) 이소골 이단례에서 염증성 부산물이 전음기전을 대행하는 것을 인정할 수 있었다. 11) 이상의 소견은 술전 고실내 병변을 추정하는 데 한 지표가 된다.