• Korean Journal of Ichthyology
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• v.24 no.4
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• pp.247-252
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• 2012

Structure on the feeding organs in two Korean ricefishes, Oryzias latipes and O. sinensis, was examined in the jaws and the teeth. In the jaw, O. latipes has a dent on the dorsal edge of distal arm of premaxilla (vs. straight in O. sinensis) and a alveolar arm of premaxilla running in straight line with distal arm of premaxilla (vs. ventra line in O. sinensis). O. latipes has also a dent on the posterior edge of dentary which is connected to coronoid process of dentary (vs. straight in O. sinensis). Large teeth developing only in the male, which is sexual dimorphism, developed into outside direction of the mandible in O. latipes (vs. dorsal direction in O. sinensis). In the teeth forms, O. latipes has two types (conical type and arrowhead type), whereas O. sinensis has only one type (conical type). Through this study, we confirmed that two Korean ricefishes show distinctive characteristics in the structure of the feeding organs.

• Animal Systematics, Evolution and Diversity
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• v.8 no.2
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• pp.177-182
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• 1992

A ricefish Oryzias latipes sinesis Chen et al. of the family Oryzidae is recorded for the first time from western Korea. The present speciment are similar to O. l. latipes in most morphometric characters, but agree well with the original description of O. l. sinensin based on the diploid chromosome number of 46 with two large metacentric chromosomes and the lesser vertebrae number.

• Journal of Ecology and Environment
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• v.36 no.3
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• pp.159-166
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• 2013

Oryzias latipes and Oryzias sinensis are indigenous species found in Japan, China, and other East Asian countries, including Korea. Based on morphological differences, the species have been classified distinctly. However, the range of morphological characters such as the number of gill rakers, vertebrae, and spots on the lateral body overlaps and is too vague for clear identification, so their classification based on their morphological characteristics remains uncertain. In this study, the mitochondrial cytochrome oxidase subunit I (COI) gene, which is used for DNA barcoding, was applied to clarify interspecific variation of O. latipes and O. sinensis. Intraspecific genetic diversity was calculated to identify correlations with geographic distributions. We studied two species collected from 55 locations in Korea. All individuals carried a 679-base pair gene without deletion or insertion. Between species, 525 base pairs of the gene were shared. The Kimura two parameter (K2P) distance of O. latipes and O. sinensis was 0.41% and 1.39%, respectively. Mean divergence within genera was 23.5%. Therefore, the species were clearly different. The distance between O. latipes and O. sinensis was 14.0%, which is the closest within genera. Interestingly O. latipes from the Japanese and Korean group represented 16.5% distant. These results were derived from geohistorical and anthropogenic environmental factors. The O. latipes haplotypes were joined in only one group, but O. sinensis was divided into two groups, one is found in the Han River and upper Geum River watershed; the other is found in the remaining South Korean watersheds. Further studies will address the causes for geographic speciation of O. sinensis haplotypes.

• Molecular & Cellular Toxicology
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• v.3 no.3
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• pp.185-189
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• 2007

Gene expression levels of choriogenin, vitellogenin and estrogen receptor were determined using Reverse transcription (RT)-PCR technique after exposure to estrogenic chemical bisphenol A in the Korean wild medaka (Oryzias sinensis). These genes have been known to be induced in male test fish when the fish are exposed to estrogenic chemicals. Therefore they can be suggested as a possible biomarker of endocrine disruption in fish, however, relatively little has been known about these genes expression by estrogenic chemicals in Korean wild fish. Mature male Oryzias sinensis were treated with bisphenol A at nominal concentrations of 0.02, 0.2 and 2 mg/L for 6 days and total RNA was extracted from the livers of treated fish for RT-PCR. When the five biomarker genes were amplified by RT-PCR in the same condition, mRNA induction level of each gene was elevated with different sensitivities. Conclusively, the results of this work indicated that measurement of vitellogenin and choriogenin using RT-PCR is effective as a simple tool for the screening of estrogenic chemicals and suggested that O. sinensis would be a suitable model fish for the environmental risk assessment of potential endocrine disruptors.

• Proceedings of the Zoological Society Korea Conference
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• 1997.10b
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• pp.155.1-155
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• 1997

• Korean Journal of Ichthyology
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• v.25 no.1
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• pp.33-37
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• 2013

A study on the structures of the pharyngeal bones and teeth in two Korean ricefishes, Oryzias latipes and O. sinensis, was carried out to find out interspecfic differences. In the pharyngeal bones, both two species have a pair of upper pharyngeal bone (stilliform shape) and lower pharyngeal bone (rectangular shape). In the lower pharyngeal bones, in particular, the number of the row was 6 to 7 in O. latipes and 5 to 6 in O. sinensis. The pharyngeal teeth also showed different types in two species: O. latipes of a top-hooked type and O. sinensis of a conical type. Through this study, we confirmed that the two Korean ricefishes show distinctive characteristics in the structure of the pharyngeal teeth.

• Korean Journal of Ichthyology
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• v.18 no.3
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• pp.163-169
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• 2006

The Korean ricefish, Oryzias, consisted of 2 species, O. latipes and O. sinensis. Through investigation on their distributions, it was confirmed that O. sinensis was restricted to western areas, and O. latipes was distributed throughout Korea. Interestingly, Daecheon region near the West Sea was sympatric, existing O. latipes and O. sinensis. In addition to their distributions, O. latipes was well distinguished from O. sinensis as the following morphological characteristics: mean 31 vertebrae (vs. 30), mean 15 gill rakers (vs. 12), lots of dots in both sides of body (vs. absent), and yellowish ventral and anal fins (vs. blackish).

• Korean Journal of Ichthyology
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• v.28 no.4
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• pp.223-228
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• 2016

The anatomy and histology of the olfactory organ in Oryzias sinensis was researched using a stereo microscope and light microscope. In the gross structure, the paired olfactory organs on the dorsal part of the head consist of two nostrils (a circular anterior nostril and a slit posterior nostril in a distance), a single olfactory chamber and a single accessory nasal sac. In the histological study, the epithelium of the olfactory chamber is classified into both sensory and non-sensory regions. The sensory epithelium consists of olfactory receptor neurons, supporting cells, basal cells and vesicles, and is islet in distributional pattern. The non-sensory epithelium is composed of stratified epithelial cells and two types of mucous cells (acidic and neutral cells). The epithelium of the accessory nasal sac has swollen stratified epithelial cells, mucous cells with a rich glycoprotein. Such an olfactory anatomy and histology of O. sinensis may reflect its habitat surrounding stagnant and polluted water.

• Journal of Wetlands Research
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• v.15 no.1
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• pp.19-24
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• 2013

To assess on the biological control potential with the native fishes, Aphyocypris chinensis, Oryzias sinensis, Misgurnus mizolepis, Pseudorasbora parva, Carassius auratus, Odontobutis interrupta were tested in relation to optimal condition controling mosquitoes in Seosan region. As a result of larvivorous activity for predation rate and temperature difference along kinds of native fishes, predation rate of Aphyocypris chinensis was the highest and predation rate of Misgurnus mizolepis was the lowest under the $23^{\circ}C$. Contrastively, predation rate of Aphyocypris chinensis and Odontobutis interrupta was the highest in least time. Predation rate of Misgurnus mizolepis was the lowest under the all temperature among six species fishes. Aphyocypris chinensis and Pseudorasbora parva in low, Carassius auratus, Misgurnus mizolepis and Odontobutis interrupta in bottom appeared high predation rate along the water depth. And predation rate of Oryzias sinensis was high as 95% at surface. As a result of measuring time taken to predate 50 mosquito larvae by 3 species fishes, two Aphyocypris chinensis took 10minutes, two Oryzias sinensis took 21min., two Misgurnus mizolepis took 45min. But one Aphyocypris chinensis and Misgurnus mizolepis took 8min., one Misgurnus mizolepis and Oryzias sinensis took 17min.

• Korean Journal of Ichthyology
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• v.5 no.1
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• pp.113-121
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• 1993

Cytogenetic studies and hybridization experiment using two populations of Oryzias latipes from Korea were conducted in order to examine their systematic positions. In the chromosome number of specimens examined. O. sinensis had 46 with a pair of large metacentrics and O. latipes had 48 chromosomes without a pair of large metacentrics. However, the diploid chromosome number of the hybrid between O. latipes and O. sinensis was 47 and they had a large metarcentric chromosome in their karyotype. In the $F_2$ hybrids, distribution of chromosome number was variable among individuals. Hatching rates showed little difference between control and hybrids. However, hatchiabilties of $F_2$ hybrids were decreased by 83.3% as compared with $F_1$ offspring. Incidence of abnormal larvae was increased by 15.3% as compared with their parents. These results indicate that the two karyotype populations formerly regarded as two subspecies would be two distinct allopatric sibling species O. sinensis, and O. latipes.