• Title/Summary/Keyword: Orthopaedic

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Biphasic effects of TGFβ1 on BMP9-induced osteogenic differentiation of mesenchymal stem cells

  • Li, Rui-Dong;Deng, Zhong-Liang;Hu, Ning;Liang, Xi;Liu, Bo;Luo, Jin-Yong;Chen, Liang;Yin, Liangjun;Luo, Xiaoji;Shui, Wei;He, Tong-Chuan;Huang, Wei
    • BMB Reports
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    • v.45 no.9
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    • pp.509-514
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    • 2012

  • We have found that the previously uncharacterized bone morphogenetic protein-9 (BMP9) is one of the most osteogenic factors. However, it is unclear if BMP9 cross-talks with $TGF{\beta}1$ during osteogenic differentiation. Using the recombinant BMP9 adenovirus, we find that low concentration of rh$TGF{\beta}1$ synergistically induces alkaline phosphatase activity in BMP9-transduced C3H10T1/2 cells and produces more pronounced matrix mineralization. However, higher concentrations of $TGF{\beta}1$ inhibit BMP9-induced osteogenic activity. Real-time PCR and Western blotting indicate that BMP9 in combination with low dose of $TGF{\beta}1$ potentiates the expression of later osteogenic markers osteopontin, osteocalcin and collagen type 1 (COL1a2), while higher concentrations of $TGF{\beta}1$ decrease the expression of osteopontin and osteocalcin but not COL1a2. Cell cycle analysis reveals that $TGF{\beta}1$ inhibits C3H10T1/2 proliferation in BMP9-induced osteogenesis and restricts the cells in $G_0/G_1$ phase. Our findings strongly suggest that $TGF{\beta}1$ may exert a biphasic effect on BMP9-induced osteogenic differentiation of mesenchymal stem cells.

  • https://doi.org/10.5483/BMBRep.2012.45.9.053 인용 PDF KSCI