• 한국의류학회지
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• 제33권8호
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• pp.1253-1261
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• 2009

The paper-folding effect of origami signifies a planarization of the body which questions the three dimensional construction of the body in a more conventional clothing system. Un-structured, variable space posited between the body and clothes, and an absence of gender identification characterizes the planarization of the body. Origami inspiration in fashion stresses a will-to-form rather than mere bodily proportion and structure, which explores a trans-extensity that goes beyond the boundary of the body. This study inquires on the influence of origami as a method to create new vestmental space in contemporary fashion. The flat-plane clothes of origami-inspired design are no longer cut to the body and the clothes could be altered by the wearer. The space is introduced in between a three-dimensional dress-body and the body. Origami-inspired design in Japanese avant-garde fashion deconstructs the convention and promotes a symbiosis of body and clothing instead. The shape of the body is completely estranged by three-dimensional sculptures, alluding to the paper folds of origami and the movement of the body that are transmitted to the clothing through fine folding that transform into an unexpected event.

• 한국의류산업학회지
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• 제11권4호
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• pp.515-526
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• 2009

From traditional ceremony to science technology, origami was used as technique for artistic creativity and functionality based on formative characteristics. The purpose of this study is to analyze the expressive characteristics of functionality in fashion design using origami technique. The method of this study was to analyze recent documentaries, fashion books, fashion magazines, internet web site and so forth. The results were as follows: In various fields of design, the category of the expressive characteristics was classified as efficient correspondence of space, compositive convergence of function, variable potentiality of form and human ergonomic optimum. In recent origami fashion based on functionality, the efficient correspondence of space was expressed constructive or decorative pocket design, using on clothing surface and varied design by lifestyle. The compositive convergence of function was expressed innovative connection of fashion items or non-fashion items and deconstructed classical clothing pattern. The variable potentiality of form was expressed control of form by irregular fold, dynamic metamorphosis by organic fold and creation of varied clothing silhouette. The human ergonomic optimum was expressed metamorphosis mechanism from two dimension to three dimension, suitable construction to physical ability and limit and organic form based on articular folding. The functional origami fashion means reflection of nomadic lifestyle, maximum of usability, pursuit of playful sensibility, creation of new body aesthetic and pursuit of technology and humanism. And the result of these study may be used for creative inspiration in fashion design because of unique characteristics.

• 한국의상디자인학회지
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• 제18권4호
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• pp.63-75
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• 2016

This study examines the organic geometry in Isabel Toledo's collections in terms of the practicality of American sportswear tradition. This study conducts literary survey combined with case analysis of Toledo's works from her debut collection in 1985 to the recent ones. The organic geometry in Toledo's designs refers to the conversion of two-dimensional garment patterns into three-dimensional garment forms with the body as a medium, which is classified into the following categories in this study. First, 'fluidity' describes Toledo's highly fluid jersey dresses which maintain consistent structures by patchwork draping and suspension technique. Second, 'reductionist structure' illustrates that simple geometric shapes such as circles and squares disappear as soon as worn on the body. Third, 'origami construction' explains folding two-dimensional fabrics into three-dimensional forms, which causes the outlines of the body to appear abstract. Toledo's designs deliver the tradition of American sportswear through the organic geometry of garment construction. Toledo's works are authentic American in the aspects that they are functional and modern; they satisfy the practical needs, prioritize the movements of wearers, pursue multi-functions, and their ornamental elements are accompanied by the construction of garments. Isabel Toledo presents designs drawing on her unwavering aesthetics while continuously developing and experimenting creative ways of garment construction.

• KSBB Journal
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• 제23권5호
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• pp.403-407
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• 2008

본 연구에서는 현재 산업적 응용이 활발하게 이루어지고 있고, 여러 장점을 지닌 효소인 Candida antarctica에서 유래된 lipase B (CalB)의 신속한 개질을 위해 취급이 용이한 E. coli를 이용하여 CalB 발현시스템을 구축하였다. E. coli 발현 시스템에서 효소활성을 지니지 못하는 내포체를 생성하는 단점을 지니고 있어, soluble한 형태의 CalB 생성을 위해 저온 발현이 가능한 pCold I vector와 단백질 접힘을 도와주는 chaperone을 사용하여 CalB를 발현하였다. Liu 등(17)은 E. coli Origami2와 B, 그리고 $DH5{\alpha}$를 실험한 결과, Origami 균주에서만 CalB에 의한 halo의 형성이 관찰되었으나, 동 연구에서는 실험한 3종의 균주와 5종의 chaperone plasmid중 Rosettagami와 $DH5{\alpha}$에서 groES/groEL chaperone이 CalB와 동시에 발현되면 soluble한 형태의 Cal B가 발현됨을 관찰할 수 있었다. 또한 신속한 CalB의 발현시스템을 구축하기 위해서는 유전자 조작의 용이성 및 안정성에서 우월한 $DH5{\alpha}$가 Rosettagami에 비해 soluble한 CalB의 발현에 더욱 적합한 균주임이 관찰되었다. 즉 재조합 pCold plasmid와 pGro7 plasmid (groES/groEL)로 형질이 전환된 $DH5{\alpha}$가 CalB 발현시스템에 가장 적합하다.

• Journal of Microbiology and Biotechnology
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• 제31권12호
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• pp.1732-1740
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• 2021

Tobacco etch virus protease (TEVp) is a useful tool for removing fusion tags, but wild-type TEVp is less stable under oxidized redox state. In this work, we introduced and combined C19S, C110S and C130S into TEVp variants containing T17S, L56V, N68D, I77V and S135G to improve protein solubility, and S219V to inhibit self-proteolysis. The solubility and cleavage activity of the constructed variants in Escherichia coli strains including BL21(DE3), BL21(DE3)pLys, Rossetta(DE3) and Origami(DE3) under the same induction conditions were analyzed and compared. The desirable soluble amounts, activity, and oxidative stability were identified to be reluctantly favored in the TEVp. Unlike C19S, C110S and C130S hardly impacted on decreasing protein solubility in the BL21(DE3), but they contributed to improved tolerance to the oxidative redox state in vivo and in vitro. After two fusion proteins were cleaved by purified TEVp protein containing double mutations under the oxidized redox state, the refolded disulfide-rich bovine enterokinase catalytic domain or maize peroxidase with enhanced yields were released from the regenerated amorphous cellulose via affinity absorption of the cellulose-binding module as the affinity tag.