• Korean Journal of Acupuncture
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• 제28권2호
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• pp.1-11
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• 2011

Objectives : The aim of this study was to examine the effect acupuncture by needle manipulation at acupoints, SP3 HT7, on the blood pressure and renin and atrial natriuretic peptide(ANP) in plasma, cardiac hypertrophy in hypertensive rats induced by two kidney one clip (2K1C). Materials and Methods : The experiments were performed on twenty-five Sprague Dawley rats, 2K1C hypertension model was prepared by constricting the left renal artery with a sliver clip. Animals were divided into five groups, control, AC-1a and AC-2a(acupuncture at SP3 HT7 bilaterally and the needle was twirled and rotated forward with the thumb and forefinger of the right hand 6 times), AC-3a(acupuncture at SP3 HT7 bilaterally and the needle was inserted in the opposite direction(body direction) as the channel runs), AC-4a(acupuncture at SP3 HT7 bilaterally and the needle was inserted in the opposite direction(body direction) as the channel runs and the needle was twirled and rotated forward with the forefinger of the right hand 6 times). The treatments were started on the 4 week after inducing 2K1C, and they were performed two times a week for 3 weeks in rats. Results : The results are that The blood pressure was significantly decreased at 4 times in Acu-1a, The cardiac hypertrophy was significantly decrease in Acu-2a and Acu-3a. The activity of plasma renin was decreased in all groups without control and Acu-1a, and that of plasma ANP was decrease in Acu-2a and Acu-3a than control group. Conclusions : These results suggest that acupuncture on SP3 HT7 mostly cause significant changes on controlling renal hypertension induced by 2K1C in the rats.

• 생명과학회지
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• 제22권5호
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• pp.650-656
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• 2012

느타리버섯의 생산단가를 절감할 목적으로 한의원 등에서 폐기처리 되고 있는 한방슬러지를 수거하여 느타리 버섯 배지의 개발을 행하였다. 한방재료의 일반성분을 검토한 결과 조회분 함량은 홍화대와 지리오갈피가 11.6%와 10.1%를 나타내어 대조구인 폐면의 9.6% 보다 높게 나타났다. 조지방 함량은 모든 시험구에서 대조구인 폐면보다 높게 나타났다. 조단백 함량은 폐면배지에서는 9.8%로 검출되었으나 야생 쑥, 지리오갈피, 한방슬러지 및 홍화대에서는 각각 14.9%, 13.9%, 13.4% 및 11.5%로 대조구보다 높게 나타났다. 한방슬러지, 야생쑥 및 지리오갈피의 pH는 5.27~5.72 범위로 폐면이 나타낸 pH 5.70과 거의 같은 수준이었다. 느타리버섯의 균사 성장에 미치는 각 한방재료의 첨가농도는 3% 및 6%의 첨가보다 9%의 첨가구에서 느타리버섯 균사의 성장이 양호하였다. 그러나 한방재료의 첨가는 $P.$ $tolaassi$의 생육 및 $Trichoderma$ $sp.$의 생육에는 크게 영향을 미치지 못하였다. 폐면배지에 9%의 한방슬러지를 첨가하여 실시한 현장 실험결과 버섯의 균사생장은 폐면배지에서 보다 약 2~3일 정도 빠르게 나타났고, 버섯의 수확량은 10~15% 정도 증수되었으며 또한 버섯의 대 및 갓 등이 균일하고 우수하였다.

• 한국식품영양과학회지
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• 제18권3호
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• pp.321-327
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• 1989

김치의 산패를 지연시킬 수 있는 천연 pH 조정제를 찾아내기 위하여 김치에 첨가 가능한 한방 및 식재료 72종의 1% 물추출액에 대하여 산에 대한 완충능을 조사하였다. 한방재료중에서 완충능이 100이상으로 나타난 것은 감초 150, 방풍 142, 등 14종이었다. 한방재료들은 50%씩 상호혼합한 것중 완충능이 150이상으로 높게 나타난 것은 소엽+감국, 소엽+산약, 소엽+갈근, 감국+갈근, 택사+갈근, 택사+일당귀, 산약+갈근이었다. 버섯류, 두류 및 채소류 중에서 완충능이 높게 나타난 것은 녹두 207, 대두 160, 완두 192, 느타리 225, 쑥갓 200, 비름 175, 깻잎 200, parsley 202, 피망 257 등이었으며, 이들을 상호혼합처리한 결과 두류상호간의 혼합을 제외하고는 완충능이 향상되었는데 특히 느타리+쑥갓 262, 느타리+parsley 255, 녹두+쑥갓 247, 대두+느타리 245, 대두+쑥갓 247, 대두+parsley 250으로 매우 높았다. 완충능이 높게 나타난 천연 pH 조정제를 무우즙액에 혼합한 결과 완충능에서 효과가 인정되는 것은 녹두+완두 272, 녹두+대두 282, 느타리+parsley 302, 쑥갓+비름 300, 쑥갓+깻잎 277, 비름+피망 300, 비름+parsley 280 등 이었으며 배추즙액에서 효과가 인정된 것은 완두 202, 느타리 202, 쑥갓 207, 느타리+parsley 205 등이었다.

• 생명과학회지
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• 제19권10호
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• pp.1478-1483
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• 2009

곡물을 소재로 혈행개선에 효과 있는 물질을 개발하기 위하여 Bacillus subtilis BK-17을 이용하여 고체 배양을 시도하였다. 고체배양에 접종하는 미생물은 영양세포보다 포자 접종이 혈전용해효소 생산에 효과적이었다. 포자 생산을 위한 배지는 SFM (0.8% nutrient broth, 0.05% yeast extract, $10^{-1}$ M $MgCl_2$, $10^{-3}$ M $FeCl_3$, $10^{-4}$ $MnCl_2$, $10^{-5}$ M dipicolic acid, pH 6.5)이 최적이었고, 포자 생성에 대한 최적 pH 와 온도는 각각 pH6과 $30^{\circ}C$였으며 포자는 배양 후 60 시간에서 가장 많은 생산을 보였다. 각종 곡물을 기질로 하였을 때 녹두가 혈전용해능이 가장 높았으며 강낭콩, 검정콩, 대두, 옥수수는 녹두의 약 90% 수준의 활성으로 높은 혈전용해능을 보였다. 반면에 보리와 밀과 같은 탄수화물 곡물은 낮은 활성을 보였다. 고체 배양한 발효 대두는 단백질과 지질 함량에 있어 발효하지 않은 대두보다 약 10% 이상의 높은 수치를 보였다. 또한 필수 아미노산과 일반 아미노산의 함량 역시 발효 대두 쪽이 약 5 - 20% 높은 것으로 보아 혈행개선기능성 뿐만 아니라 식품으로 실용 가능성이 높은 것으로 사료된다.

• 한국자원식물학회지
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• 제18권3호
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• pp.359-366
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• 2005

재배종담배(Nicotiana tabacum L. cv.), 야생종담배 (N. plumbaginifolia)와 벼종자(Orya sativa L, cv.)에 neutron빔을 각각 조사(irradiation)하여 발아, 식물체의 생장과 DNA의 변이에 미치는 영향을 검토하였다. 담배종자와 벼종자에 90, 180, 270, 360, 450, 540 Gy까지 조사로 발아율은 크게 감소하지 않았고, 생장에 있어 유의적인 차이는 없었다. 그러나 담배식물체에서 총 200개체 중 71개체(약 $36\%$)가 잎에서 형태 이상을 나타내었다. 또한 줄기색 변이체, 엽색변이체, 화형변이체를 유도하였다. 이 결과는 neutron빔이 유용한 돌연변이원으로서 가능성이 있음을 시사해 준다. Neutron빔을 조사한 후 생장한 담배식물체의 잎에 대하여 총 34개의 primer를 이용하여 RAPD 분석한 결과 20개의 primer에서 총 104개의 DNA 단편이 증폭되었고, 중성자 빔조사처리구에서만 출현하는 DNA 단편은 나타나지 않았다.

• Korean Journal of Acupuncture
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• 제31권3호
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• pp.125-135
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• 2014

Objectives : Warm needling is the method combining the effects of acupuncture and moxibustion. In modern clinics, warm needling is only affected by treatment time or frequency. We need to study the physical characteristics of warm needles currently used in clinics in order to develop more efficient and economical treatments. Methods : We collected various warm needle specimens and analyzed chemical constitutions, measured heat transfer velocity, micro Vicker's hardness and specific resistance. We studied the relationship between heat transfer velocity and micro Vicker's hardness as well as that between heat transfer velocity and specific resistance. Results : The heat transfer velocity of the Silver_HL was 3.3 mm/sec, of the Au alloy group was 1.3~2.6 mm/sec, and Silver_IN, Silver_ZK and SS groups was less than 0.3 mm/sec. We therefore concluded that the needle composed of Ag has the best heat transfer velocity. In the Micro Vicker's hardness test, Vicker's hardness of the Au alloy group was 159~170 Hv, of Silver_HL was 181 Hv, and of the Silver_IN, Silver_ZK, SS group was 450.8~519 Hv. In the Silver_IN, Silver_ZK and SS groups, hardness was inversely proportial to thermal conductivity. In the specific resistance test, the specific resistance of Silver_HL was the lowest, that of the Au alloy group was the second lowest, and that of the Silver_IN, Silver_ZK, SS groups were the highest. Conclusions : We concluded that the needle composed of Ag has the best heat transfer velocity, highest electric conductivity and thermal conductivity, therefore the needle composed of Ag is suitable for warm needling.

• 대한한방소아과학회지
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• 제34권2호
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• pp.75-85
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• 2020

Objectives Although clinical practice guidelines (CPGs) are becoming crucial in medical decision-making worldwide, there are very few development guidelines for children. Therefore, this preliminary study aimed to assess the status of worldwide except east asia-CPGs recommending complementary and alternative medicine interventions (CAM-i) in children worldwide except east asia in order to help developing Korean medicine CPGs (KMCPGs) for children. Methods To identify the current status of CPGs including CAM-i that have been developed so far, the author searched the related databases, and selected CPGs for children. The data related to the status of evidence-based CPGs, the materials related to the characteristics of CAM-i, and the details of recommendations for CAM-i were selected from the obtained findings. Results A total of 106 CPGs were identified based on the searches, of which 11 were finally selected as pediatric CPGs. These pediatric CPGs have been developed since 2007 to target various diseases. The countries developing these CPGs include the United Kingdom, Scotland, Australia, and the United States, with the U.K. having the largest share. The majority of CPGs used MEDLINE, Embase, and Cochrane library as databases. The most frequently recommended topic was acupuncture, followed by herbal medicine. Many CPGs suggested that more research was needed. Conclusions Through this study, the author was able to identify the characteristics of the developed pediatric CPGs that include CAM-i. More preceding studies are needed for the development of pediatric KMCPGs. It is hoped that this report can be used as the foundation for future development of pediatric KMCPGs.

• Asian Pacific Journal of Cancer Prevention
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• 제16권6호
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• pp.2317-2322
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• 2015

Background: Despite the increasing number of screening examinations performed for cervical cancer utilizing the Papanicolaou smear test (Pap test), few studies have examined whether this strategy is cost-effective in Korea. Objective: This study was conducted to evaluate the cost-effectiveness of cervical cancer screening strategies incorporating the Pap test based on age at the start and end of screening as well as screening interval. Materials and Methods: We designed four alternative screening strategies based on patient age when screening was started (20 or 30 years) and discontinued (lifetime, 79 years). Each strategy was assessed at screening intervals of 1, 2, 3, or 5 years. A Markov model was developed to determine the cost-effectiveness of the 16 possible cervical cancer screening strategies, and this was evaluated from a societal perspective. The main outcome measures were average lifetime cost, incremental quality-adjusted life-years (QALYs), and incremental cost-effectiveness ratio (ICER). Results: Compared with various strategies comprising younger starting age, discontinuation age, and longer screening intervals, strategies employing annual screening for cervical cancer starting at a target age of 30 years and above were the most cost-effective, with an ICER of 21,012.98 dollars per QALY gained (with a Korean threshold of 30,000,000 KRW or US$27,272). Conclusions: We found that annual screening for cervical cancer beginning at a target age of 30 years and above is most cost-effective screening strategy. Considering the potential economic advantages, more intense screening policies for cervical cancer might be favorable among countries with high rates of cervical cancer and relatively low screening costs.

• Asian Pacific Journal of Cancer Prevention
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• 제17권9호
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• pp.4301-4306
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• 2016

Background: Cancer is a burden on humanity and ranks as a leading cause of morbidity and mortality in China. Shanxi province has its unique cancer patterns and the burden is increasing. In this study, we aimed to assess the pattern of dietary habits and life-style in Shanxi, a high-risk area for upper gastrointestinal cancers in China and further evaluate the trends in cancer incidence and mortality based on registered data. Materials and Methods: Data on lifestyle, diet, physical activity were obtained from the household health survey at Zhongyang from 2013 to 2015. Cancer diagnoses were reported to Shanxi Center for Disease Control and Prevention (SCDCP). Population-based cancer incidence data and mortality data of 2012 were collected from the SCDCP. All incidence and death rates were expressed per 100,000 populations. Univariate analysis was performed using the Chi-squared test or Fisher's exact test. Results: Overall, deficiencies in fresh fruits and vegetable food, and intake of hot food, salted food, or pickled food are serious problems in Shanxi, especially in rural areas. Upper gastrointestinal cancers were the most commonly diagnosed cancers, and the incidence in rural areas is higher than those in urban areas. Cervical cancer is the most common cancer for females. Moreover, the agespecific incidence exhibited an increased trend before 40 years old. Consistent with the previous literature, our epidemiological investigation results suggest that lifestyle, nutrition deficient, and infections were major risk factors for upper gastrointestinal cancers or cervical cancer in Shanxi. Facing a serious situation, we further explored defensible recommendations for the general public in order to promote changes in environments that support healthful eating and physical activity habits, to reduce cancer risk. Conclusions: Our results present the current cancer trends in Shanxi and its related etiologic risk factors and provide a theoretical basis to guide public health efforts to prevent and control cancers in the province.

• 한방안이비인후피부과학회지
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• 제29권3호
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• pp.124-133
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• 2016

Objectives : Recently, the demands for the effective and safe depigmentating and anti-aging agents of the skin have increased due to the medical, pharmaceutical and cosmetic reasons. The aim of this study is to search new materials from the traditional herbal medicines which inhibit the aging process of skin in vitro.Methods : Human dermis cell (HS68) was used to test the effect of Rhus verniciflua Stokes (RVS). 80% ethanol or water extracts were screened for their inhibitory activities against elastase. Elastase inhibition effect was tested by microplate reader instrument. And MMP-1 suppression effect of RVS was tested by western blot. These cells were investigated the viability by MTS assay. And also the inhibition effect of tyrosinase by RVS was tested.Results : RVS (final concentrstion 1 ㎎/㎖ appeared over 30% of inhibition of elastase activity. So we are investigated anti wrinkle effects of Rhus verniciflua Stokes look through MMP-1 inhibition activity, also Extracts of RVS showed higher anti-tyrosinase activity than arbutin as final concentration 1 mg/ml. These results suggest that herbal medicines could be strong potential sources of inhibition of anti-aging and whitening effects for the skin.Colclusions : RVS was the best suppressor candidate of elastase activity among other control oriental drugs. It was found that RVS did not have toxicity to cells. We found proper concentration of RVS to treat in HS68 culture and investigated not only wrinkle care effect but also whitening effect of RVS.