• Journal of Microbiology and Biotechnology
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• v.4 no.3
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• pp.204-209
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• 1994

The synthesis of N-(benzyloxycarbonyl)-L-aspartyl-L-phenylalanine methylester (Z-APM), a precursor of aspartame, from N-(benzyloxycarbonyl)-L-aspartic acid (Z-Asp) and L-phenylalanine methylester hydrochlolide($L-PM\cdot HCI$) was investigated in a saturated-ethylacetate single phase system using immobilized thermolysin. Among the various supports tested, glyceryl-CPG was found to be most efficient for retaining enzyme activity. The enzyme immobilized onto glyceryl-CPG also showed the highest activity for Z-APM synthesis in saturated ethyl acetate. Z-APM conversion yield in saturated ethylacetate was half of that obtained in an ethyl acetate-buffer two-phase system under the same reaction conditions. However, as the mole ratio of $L-PM \cdot HCI$ to Z-Asp was increased to 4.0, the conversion yield reached 95 %. When continuous synthesis of Z-APM was canied out in a plug flow reactor (PFR) with 80 mM of L-PMㆍHCI and 20 mM of Z-Asp in saturated ethylacetate (pH 5.5), more than 95 % of Z-Asp was converted to Z-APM with a space velocity of 1.16 $hr^{-1} at 40^{\circ}C$. Although the operational stability in PFR was reduced rapidly, more than 80% of initial activity was maintained in CSTR even after a week of operation.

• Journal of the Korean Chemical Society
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• v.38 no.6
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• pp.449-455
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• 1994

In order to obtain the basic data for synthetic studies of bioactive peptide using enzyme, Kyotorphin(analgesic peptide) derivative was synthesized from Ac-Tyr-OH and $Arg-NH_2$ by $\alpha-chymotrysin$ in two phase system(organic phase and aqueous phase). In effect of organic solvent on Kyotorphin derivative synthesis from Ac-Tyr-OH(10 mM) and $Arg-NH_2$ (20 mM), the synthesis in ethyl acetate system of organic solvents was higher than those in other organic solvents (n-butanol, n-hexane, dichloromethane and chloroform). The optimal conditions for the synthesis are as follows: enzyme conc., 10 ${\mu}M;$ reaction pH, 7.0; reaction temp., $35^{\circ}C$ ; the ratio of organic phase volume/aqueous phase volume $(\alpha)$, 15. Under the optimal conditions, the yield was 70.2%, and the reaction achieved to equilibrium after 24 hrs.

• KSBB Journal
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• v.5 no.4
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• pp.411-414
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• 1990

The extraction behavior of an alkaline protease using reversed micelles was investgated. The reversed micellar solution consisted of AOT in isooctane. It was found that distribution of arkaline protease into the organic phase increased at lower pH, lower ionic strength, and higher AOT concentration. When the real fermentation broth was extracted of alkaline protease, an activity yield of 20% and a purification factor of 2.0 were obtained.

• Applied Chemistry for Engineering
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• v.9 no.4
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• pp.475-480
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• 1998

A study on the electrochemical reduction of uranium (VI) to uranium (IV) was carried out in the mixed phases of an organic phase with uranium (VI) and aqueous phase of nitric acid by use of a highly packed glassy carbon (GC) fiber column electrode system, and a model for in-situ electrolytic stripping of uranium (VI) was suggested. The electrochemical reduction of uranium (VI) occurred faster in organic phase than in aqueous phase of the mixed phases. The uranium stripping yield increased and then became constant with the increase of organic flow rate of the electrolytic system due to the increase of diffusion resistance of uranium ions in the organic phase into the aqueous phase. Aqueous flow rate, on the other hand, didn't affect the total uranium (VI) reduction current in the system. The system combined with electrochemical reduction was confirmed to be much more effective than the simple system without it in stripping uranium.

• Macromolecular Research
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• v.17 no.5
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• pp.313-318
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• 2009

The side chain liquid crystal triblock copolymers (TBCs), which underwent phase transitions below their decomposition temperature, were prepared by copolymerization of poly(n-butyl acrylate) and a comonomer containing the mesogenic azobenzene group. The physical properties of TBCs in the distinctive transition temperature ranges were investigated in terms of the liquid crystal (LC) content in the copolymers. The phase transition temperatures traced optically, thermally and rheologically were well coincided one another and clearly exhibited the phase transition of smectic-nematic-isotropic with increasing temperature. In the smectic phase, increasing temperature made the liquid crystal system more elastic, but viscosity (${\eta}'$) remained almost constant. In the nematic phase, increasing temperature abruptly decreased ${\eta}'$ and G', ultimately leading to isotropic phase. Both smectic and nematic phases exhibited Bingham viscosity behavior but the former gave much greater yield stress at the same LC content.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.1
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• pp.64-71
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• 2018

To improve the soil of reclaimed land, we added organic materials at a level of 3,000 kg/10 a. As a result, the electrical conductivity (EC) value of reclaimed soil decreased by 58%, the organic material content increased from 6.7 to 16.0 g/kg, the porosity increased from 1.57 to 1.31%, the soil hardness decreased from 20.2 to 17.9 mm and the plow layer was deepened from 19.8 to 26.8 cm. After these physiochemical improvements to the reclaimed soil, the growth phase of crops was improved compared to that of non-treatment crops. The height of kenaf (Hibiscus cannabinus L.) cultivated in the reclaimed land containing organic materials was increased by 18.8%. Especially, the improvement effects of pellet type manure compost and rice straw on kenaf were more preferable than those of other organic materials. When the kenaf was cultivated in the reclaimed land containing organic materials, the yield increased. The average yield of the treatment crops was 9,218 kg/10 a, 2.1 times higher than that of non-treatment crops. The most effective treatments to increase the yields were pellet type manure compost (10,848 kg/10 a, 148% increase), rice straw (120% increase) and chopped kenaf (95% increase). To increase the physicochemical enhancements to the reclaimed land soil and most improve yields, the most effective type of organic materials was the pellet. The organic material types that maintained a better growth phase and most increased the yield were the liquid and pellet types. When we used pellet type organic material, the plant height of kenaf was increased by 41% in comparison with that of the non-treatment crops and yield was increased by more than 122%. Additionally liquid type organic material improved the yield (by 127%).

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 2006.06b
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• pp.317-321
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• 2006

The investigation of the formation of organic sulfur compounds (i.e., odor species), mainly methyl mercaptan and demethyl suifide, during kraft pulping has been conducted, in which both hardwood and softwood species were used. It was discovered that there is an organic sulfur compounds formation phase transition point with respect to delignification extent. The transition point occurs at a kappa number of approximately 35 and 20 for softwoods and hardwoods, respectively. The results also showed that both low sulfidity and anthraquinone (AQ) addition are helpful to control the formation of theses organic sulfur compounds during kraft pulping. Whereas Hexenuronic acid (HexA) has contribution to Kappa number, the extended delignification not only lead to low pulp yield, but also be adverse to odor control. A significant in-digester odor reduction can be achieved if the pulping is to be terminated before phase transition point and combined with AQ addition.

• Journal of the Korean Society for Marine Environment & Energy
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• v.3 no.3
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• pp.34-40
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• 2000

A multisample extraction device was newly designed for efficient extraction of dissolved lipophillic organic compounds from sea water sample. This device allowed extraction of organic compounds from up to 96 sample at a time using 96 multifolder on the principle of solid phase extraction with commercially available octadecyl silane (ODS) cartridges. The recovery yield of the new divice was higher than 90 % while that of conventional liquid-liquid extraction process are only 60 - 70 %. The amount of solvent required for the new device could be reduced to less than 20㎖ per 1ℓ of sample while 1 - 2 ℓ of solvent were used in the conventional liquid-liquid extraction process. The usefulness of this novel method was demonstrated with sea water samples collected from Yellow sea, and the qualitative and quantitative analyses results of the dissolved hydrocarbon showed this method was superior to that of conventional liquid-liquid extraction process in efficiency and reliability.

• Nuclear Engineering and Technology
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• v.5 no.3
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• pp.202-213
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• 1973

In a continuation of the 1st series of these studies, a separation of the components in organic phases obtained from Szilard Chalmer reactions of aromatic chloro derivatives was attempted to clarify the conditions of isolating the main component. Though it was unable to isolate all constituents of organic phases, the main component was well isolated by means of a simple distillation or a thin layer chromatography. It was identified that the main component was a recombined product of the recoiled $^{38}$ Cl atom with the original sample. In cases of solid samples the main component amounted about 80-60% of the radio activity of the organic phase, whereas the main component from liquid samples gave less than 70% of the radio activity of the organic phase. A prolonged irradiation could increase the yield of a main component, but it was, in case of a radiation senintive compound, decreased as the irradiation is extended. The number of aide produets separated from an organic phase of a liquid sample was greater than that of a solid sample, and hence the main component of a solid sample was isolated easier. The overall fields of the labelled main products of 22 kinds of compounds were listed and a procedure of the separation of the main component by a thin layer chromatograply was proposed and evaluated. The feasibility of the present procedure for labelling purpose was also reviewed with respects to the chemical structure of a compound.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.2
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• pp.108-115
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• 1991

A field experiment was conducted to evaluate the effects of cattle compost application on the change of soil physical properties and their relationship to yield performance of selected main forage crops. Maize(CV. Suweon 19) and sorghum hybrids(CV. Pioneer 9'31) as a summer crops and winter rye were grown on newly reclaimed red yellow soils(Fine loamy, Typic Hapludults) under different application rate of cattle compost associated with chemical NPK fertilization, from Oct. 1986 to Sept. 1989. Experimental field was laid down as a split plots design with four replications. The results obtained are summarized as follows: 1. Cattle compost application reclaimed soil physical propeties, such as formation of granular structure and water holding capacity, and it result in a great increase of plant growth and the rate of dry matter accumulation. 2. While cattle compost treatment reduced the portion of soild phase of the three phase constituents of soils, it increased the portion of air phase and liquid phase comparatively. 3. Organic matter, N, P, K, and mineral content in soil were markedly increased in the plot treated with compost. 4. Cattle compost application increased fodder production both in maize-rye and sorghum hybrids-rye cultivation. Annual dry matter yield of maize-rye cropping was 2183(NI'K only), 2425(NPK+compvst 3000 kg) and 2800kg/lOa(NPK + compost 6000kg/10a).