• Title/Summary/Keyword: Oral state

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Comparison of LIVE/DEAD$^{(R)}$ BacLight$^{TM}$ Bacterial Viability Test and alamarBlue$^{(R)}$ Method for Enumeration of Live and Dead Bacteria for Oral Bacterial Species

  • Kim, Yeon-Hee;Lee, Si Young
    • International Journal of Oral Biology
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    • v.37 no.4
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    • pp.197-201
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    • 2012
  • LIVE/DEAD$^{(R)}$ BacLight$^{TM}$ and alamarBlue$^{(R)}$ are fluorescent materials used for the enumeration of live and dead bacteria. LIVE/DEAD$^{(R)}$ BacLight$^{TM}$ is generally used for confocal microscopy applications to differentiate live from dead bacteria in a biofilm or planktonic state. AlamarBlue$^{(R)}$ has also been used widely to assay live and dead bacteria in a planktonic state. Whilst these materials are successfully utilized in experiments to discriminate live from dead bacteria for several species of bacteria, the application of these techniques to oral bacteria is limited to the use of LIVE/DEAD$^{(R)}$ BacLight$^{TM}$ in biofilm studies. In our present study, we assessed whether these two methods could enumerate live and dead oral bacterial species in a planktonic state. We tested the reagents on Streptococcus mutans, Streptococcus sobrinus, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Enterococcus faecalis and found that only LIVE/DEAD$^{(R)}$ BacLight$^{TM}$ could differentiate live from dead cells for all five of these oral strains. AlamarBlue$^{(R)}$ was not effective in this regard for P. gingivalis or A. actinomycetemcomitans. In addition, the differentiation of live and dead bacterial cells by alamarBlue$^{(R)}$ could not be performed for concentrations lower than $2{\times}10^6$ cells/ml. Our data thus indicate that LIVE/DEAD$^{(R)}$ BacLight$^{TM}$ is a more effective reagent for this analysis.

Effects of Occlusal Factors and Life Event Changes on Temporomandibular Disorders (측두하악장애에서 교합요인과 생활변화의 영향)

  • You-Me Lee;Kyung-Soo Han
    • Journal of Oral Medicine and Pain
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    • v.19 no.2
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    • pp.181-192
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    • 1994
  • There have been many different theories on the etiology of temporomandibular disorders(TMDs). The objective of the study was to investigate the effects of occlusal fctors and recent life event changes as prediposing fctor on the development of temporomandibjlar disorders. To evaluate the above predisposing factor, the author used T-scan system(Tekscan Co. U.S.A.) for quantitative occlusal analysis, clinical examination for occlusal state and Social Readjustment Rating Scale(SRRS) for recent life event change units (LCU). 63 patients with TMDs and 57 patients with malocclusion presented at Wonkwang University Dental Hospital participated in this study. The subjects were grouped by Angle's classification and presence of absence of TMDs and parafunctional oral habits. Data gained with regard to contact number, contact force, contact time, occlusal state(number of total teeth and occluding teeth, overjet, overbite) and occlusal interferences (protrusive posterior contact, nonworking side interference, and RCP-ICP slide) and recent life event changes. The data were processed and analysed by SAS statistical package program, The results of this study were as follows : 1. There were no significant differences on both quantitative occlusal contact analysis and occlusal state between TMDs group and Angle's malocclusion group. Also, there were no differences among the Angle's classifications. But amount of overjet in TMDs group were more greater than that of malocclusion group. 2. There was no difference on protrusive posterior contact, and balancing contact between TMDs group and Angle's malocclusion group. Premature contact was more frequent in malocclusion group, but RCP-ICP slide was more frequent in TMDs group. And RCP-ICP slide was more freqent in Angle's class II malocclusion than Angle's I or III malocclusion. 3. Life changes units in TMDs group were higher than those in malocclusion group. And recent life change units in group with parafunctional oral habit were higher than those in group without parafunctional oral habits. Clenching was the most common habit among parafunctional oral habits.

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Quantitative assessment of image artifacts from root filling materials on CBCT scans made using several exposure parameters

  • Rabelo, Katharina Alves;Cavalcanti, Yuri Wanderley;de Oliveira Pinto, Martina Gerlane;Melo, Saulo Leonardo Sousa;Campos, Paulo Sergio Flores;de Andrade Freitas Oliveira, Luciana Soares;de Melo, Daniela Pita
    • Imaging Science in Dentistry
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    • v.47 no.3
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    • pp.189-197
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    • 2017
  • Purpose: To quantify artifacts from different root filling materials in cone-beam computed tomography (CBCT) images acquired using different exposure parameters. Materials and Methods: Fifteen single-rooted teeth were scanned using 8 different exposure protocols with 3 different filling materials and once without filling material as a control group. Artifact quantification was performed by a trained observer who made measurements in the central axial slice of all acquired images in a fixed region of interest using ImageJ. Hyperdense artifacts, hypodense artifacts, and the remaining tooth area were identified, and the percentages of hyperdense and hypodense artifacts, remaining tooth area, and tooth area affected by the artifacts were calculated. Artifacts were analyzed qualitatively by 2 observers using the following scores: absence (0), moderate presence (1), and high presence (2) for hypodense halos, hypodense lines, and hyperdense lines. Two-way ANOVA and the post-hoc Tukey test were used for quantitative and qualitative artifact analysis. The Dunnet test was also used for qualitative analysis. The significance level was set at P<.05. Results: There were no significant interactions among the exposure parameters in the quantitative or qualitative analysis. Significant differences were observed among the studied filling materials in all quantitative analyses. In the qualitative analyses, all materials differed from the control group in terms of hypodense and hyperdense lines (P<.05). Fiberglass posts did not differ statistically from the control group in terms of hypodense halos(P>.05). Conclusion: Different exposure parameters did not affect the objective or subjective observations of artifacts in CBCT images; however, the filling materials used in endodontic restorations did affect both types of assessments.

Comparison of Oral Care Interventions on the Oral Status of Intubated Patients in Intensive Care Units (기관내관을 삽입 받은 중환자의 구강간호 방법에 따른 구강상태 비교)

  • Park, Jin-Hee;Sohng, Kyeong-Yae
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.17 no.3
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    • pp.324-333
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    • 2010
  • Purpose: The purpose of this study was to evaluate the effects of three different oral care treatments on the oral state of patients with intubation in intensive care units. Methods: The research design was a nonequivalent control group design with repeated measures. The patients were assigned a normal saline, chlorhexidine or toothbrushing group. Each group received its own oral care treatment for 5 minutes, twice a day and for 8 days. The oral assessment guide, hygiene performance index and pathogenic microorganisms. Data were collected from patients before the experiment, 4 days after, and 8 days after completion and were evaluated. Results: The chlorhexidine group and tooth brushing group showed significant improvement on the oral assessment guide and decrease in the hygiene performance index, compared to the normal saline group. Similarly, pathogenic microorganisms were significantly decreased in the chlorhexidine group and tooth brushing group, when compared to the normal saline group. Conclusions: Oral treatments with chlorhexidine and toothbrushing improve the oral health state of patients, therefore use of chlorhexidine and toothbrushing could be an effective nursing intervention for intubated patients in intensive care units.

Anxiety before dental surgery under local anesthesia: reducing the items on state anxiety in the State-Trait Anxiety Inventory-form X

  • Koga, Sayo;Seto, Mika;Moriyama, Shigeaki;Kikuta, Toshihiro
    • Journal of Dental Anesthesia and Pain Medicine
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    • v.17 no.3
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    • pp.183-190
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    • 2017
  • Background: It is important to evaluate preoperative anxiety and prepare sedation when performing dental surgery under local anesthesia. Spielberger's State-Trait Anxiety Inventory (STAI) is useful for predicting preoperative anxiety. State anxiety is defined as a subjective feeling of nervousness. Reduction in the number of the state anxiety items (questions) will be clinically important in allowing us to predict anxiety more easily. Method: We analyzed the STAI responses from 1,252 patients who visited our institution to undergo dental surgery under local anesthesia. Multiple linear regression analysis was conducted for 9 groups comprising anxiety level determinations using the STAI; we then developed a coefficient of determination and a regression formula. We searched for a group satisfying the largest number of requirements for regression expression while setting any necessary conditions for accurately predicting anxiety before dental surgery under local anesthesia. Results: The regression expression from the group determined as normal for preoperative state anxiety was deemed the most suitable for predicting preoperative anxiety. Conclusion: It was possible to reduce the number of items in the STAI by focusing on "Preoperative anxiety before dental surgery."

A analysis on oral health behaviors of High School students in Gyeonggi (경기지역 일부 고등학생들의 구강건강 행태 분석)

  • Choi, Young-Suk;Jun, Bo-Hye
    • Journal of Korean society of Dental Hygiene
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    • v.12 no.6
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    • pp.1221-1231
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    • 2012
  • Objectives : The purpose of this study was to examine the oral health behavior and examine closely the connection factor and plans oral health promotion. Methods : This survey was conducted on 370 high school students in Kyunggi-do Suwon from November 21 to 23, 2011. 352 questionnaires were collected and analyzed. The collected data was analyzed using the statistical package SPSS WIN 15.0 using frequency, mean and standard deviation analysis, T-test, one-way ANOVA and Duncan's test correlation analysis. Results : The results state that 73.3% of students believe brushing teeth is very important and 26.7% of the students method of tooth brushing is up and down. for correct teeth brushing, they think choosing a brush is important(70.7%) also their brush shapes were uneven form(44.3%) and flat form(43.5%). 31.4% of students are using an oral hygiene device and 31.4% of students had received oral health education. 42.7% of female students get information about oral health from their dental clinics. 24.8% of male students receive information on oral health from parents and relatives. They think the best educator for oral health education will be a Dental hygienist(46.5%), or Dentist(48.0%). we found that a correlation between oral health interest, importance, knowledge and condition effect each other(p<.001). It means that if students have high oral health interest, oral care importance and oral health knowledge which can improve their oral health condition. Conclusions : The results of this study state that we need continuous oral health education systems and prevention programs to raise awareness among students and for the improvement of their oral health.

The study on the state of the elderly oral health and food habits (노인의 식습관과 구강건강상태에 관한 연구)

  • Gwon, Mi-Young;Won, Young-Soon;Kim, Yoon-Shin
    • Journal of Korean society of Dental Hygiene
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    • v.9 no.2
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    • pp.13-24
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    • 2009
  • The purpose of this study was to examine the relationship between the dietary habits and oral health of elderly people in an attempt to pave the way for the development of oral health promotion programs geared toward improving the quality of life of the elderly. The subjects in this study were senior citizens who were selected by convenience sampling from Seoul. The findings of the study were as follows: 1. Regarding subjective oral health state, 54.5% of the elderly people, more than the half, considered their mouth to be in good health. The number of their mean remaining permanent teeth was 13.71. 47.5 percent of the senior citizens investigated had no shaking teeth. 2. Those who found themselves to be in good oral health had meals on a regular basis(p=0.022) and ate detergent food often. The gaps between them and the others was significant(p=0.005). In contrast, the elderly people who were in a bad oral health frequently ate cariogenic food(p=0.044). 3. The elderly people who had 21 teeth or more ate detergent food often(p=0.029), and those who owned no teeth had a sweet teeth(p=0.003), ate more cariogenic food(p=0.001) and had a snack frequently(p=0.026). 4. The subjective oral health status had a positive correlation to detergent food intake(r=0.23) and had a slightly negative correlation to preference for sweets(r=-0.14), cariogenic food intake(r=-0.14) and snack intake(r=-0.06). The number of tooth was positively correlated to detergent food intake(r=0.23) and negatively to preference for sweets(r=0.32), cariogenic food intake(r=-0.30) and snack intake(r=-0.21). The presence or absence of shaking teeth had a positive correlation to snack intake(r=0.14). The above-mentioned findings suggested that the dietary habits of the elderly people had a statistically significant relationship to subjective and objective oral health state, which indicated that there was a close relationship between oral health and dietary habits. Therefore how to improve dietary habits as well as oral health should be taken into account when oral health promotion programs are developed for the sake of the elderly. That would contribute to promoting the oral health of elderly people and eventually boosting their quality of life.

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A Study on the Relationships of Oral Status and Self-perceived Oral Health Status in Elderly (일부 노인의 구강내 상태와 자가인지 구강건강상태와의 관련성)

  • Park, Jong-Hee
    • Journal of Technologic Dentistry
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    • v.35 no.2
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    • pp.145-155
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    • 2013
  • Purpose: The recent proliferation of the aging population, oral health care is closely related to the mental health of the elderly is very important being recognized. Provide basic data for improving the quality of life for the elderly, to determine whether the oral health of the elderly whether oral self-perception of health and any related wish. Methods: Surveys to May 20 from April 15, 2011, subjects were elderly and living in Daejeon and Chungnam 277 people were examined. All the statistics using SPSS 18.0 for the determination of statistical significance, and the significance level was 0.05. Results: 1. Recognized, the most common oral health, self-aware bad the state (46.2%) there was no significant difference in the general characteristics according to self-Oral health status, remaining natural dimensions or the intra-oral prosthetic, there was no significant difference. More teeth, residual phase, in accordance with intra-oral prosthetic mandibular Not all dentures, partial dentures, full dentures net self-perception of oral health status better recognized. 2. According to the type of partial dentures, oral health status self-phase, mixed mandibular removable, fixed, fixed, removable partial dentures in order to recognize that good oral health, self-aware state, showed between them, there was no significant difference. 3. Of variable dimension that has nothing to do with the remaining natural maxillary and mandibular prosthetic maxillary prosthetic mandibular partial dentures, age income, a positive correlation was negatively correlated. The case of the mandible, the mandibular prosthetic mandibular partial denture, maxillary prosthetic, the amount of income were correlated. 4. Intra-oral Prosthetics correlation variables in the maxillary, mandibular prosthesis, the positive correlation between income, age was negatively correlated, if the income of the mandible. Maxillary partial denture has nothing to do with the variable portion of the mandibular denture, age was a negative correlation was found. Conclusion: Satisfaction and how now it is important simply to live as long, but you need to pay attention to the quality of life, sometimes with an aging population and the increase of soybean To buy the loss of teeth causes the typical health problems of the elderly. Elderly people of any relevant oral health and the oral cavity by the state to determine whether to provide the basic data for oral health education was more residual value, intra-oral prosthetics there is no more self-aware, the oral health status is good to recognize that appeared. Strengthen the oral health education to improve the quality of life of the elderly, and is thought to be necessary to increase the number of residual.

A study on oral health state of immigrant workers with DMFT-index (외국인 이주 노동자의 구강건강관리 실태 및 우식경험영구치지수)

  • Kim, Ju-Yeong;Jung, Myung-Hee
    • Journal of Korean society of Dental Hygiene
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    • v.7 no.2
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    • pp.123-133
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    • 2007
  • This document is about immigrant worker who use counseling office for human rights in the Daegu Gumin Church. We researched their oral health state so that we can understand their situation and support them properly. General characteristics of study subjects, habits related to oral health, the oral examination and treatment clinic, participated in oral heath education program were studied by designed administered questionaire. And decay, filling(treatment teeth), missing tooth(lost teeth by dental-caries)was counted by oral examination, and calculated DMFT-index. This study was done from the July, 9, 2006 to Aug. 8, 2006. In a total of 289 immigrant worker, 77.9% of them were men and 22.1% of them were women. 55.7% of their salary was from 1,000,000 won to 1,500,000 won and most of them were working for a fiber industry. Many of them are living in korea for more then three years. DMFT index for men was 2.77 and for women was 4.06 so average of DMFT index was 3.06. 46.7% of them said that they are healthy in oral health state. The question for having difficulty using dental clinic in korea, 65.1% of them said "it is difficult". First reason was a communication problem and second was time. Most of them didn't have a oral health education but 85.1% of them said that they are looking forward to attending oral health education. Immigrant worker had better DMFT index then that of korean blue color worker. But still it is quite difficult for them using dental clinic in korea also cost. It is necessary to support them properly that medical insurance system, medical facilities of quality, medical insurance subscriber beside, made by their language, manual for them. At once, medical service improvement a policy is necessary for immigrant worker in korea.

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A survey about the use of oral hygiene device and awareness some of the middle age people (일부 중·장년층의 구강위생용품 인지와 사용실태)

  • Jung, Gi-Ok
    • Journal of Korean society of Dental Hygiene
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    • v.9 no.4
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    • pp.580-592
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    • 2009
  • Objectives : This study had been performed for Respondents who live in Daegu and Kyungbuk province Age group of 30 to 50 years old. Methods : The oral health state and oral heath care, dental treatments about the use of oral hygiene devices were obtained through self-administering questionnaires from 2 to 31, January, 2009. Results : 1. The toothpick was well known for respondents and automatic brush, gargle, dental floss were practically used. 2. 57.1% of man know oral hygiene device, they know more about oral hygiene device if they have higher degree, and they use more if they are older than others. 3. 55.5% of respondents who think their oral health condition is not healthy enough recognize oral hygiene device, 77.5% of respondents who visited dental clinic around six month recognize oral hygiene device. 82.4% of respondents who had been follow-up. 86.1% of respondents who think their oral health state is good enough doesn't use oral hygiene device, oral hygiene device was used more for respondents who visited dental clinic frequently. 47.1% of respondents who visited dental clinic periodically use oral hygiene device. 4. Respondents who experience implant recognize more about oral hygiene device and periodontal Tx, orthodontics problem, preservative treatment were next. 5. 60.0% of respondents who were educated tooth brushing method recognize it. Oral hygiene device was frequently used if tooth brushing time were increased. 26.4% of respondents who were changed their tooth brushing method used oral hygiene device. Conclusions : Their recognition level was practically low whose age is around 30 to 50. Their oral hygiene device use ratio is higher then others who were educated tooth brush method so that I think we need to recommend for use oral hygiene device and use method.

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