• The Korean Journal of Physiology and Pharmacology
• /
• 제21권2호
• /
• pp.161-168
• /
• 2017

Understanding the crosstalk mechanisms between perivascular cells (PVCs) and cancer cells might be beneficial in preventing cancer development and metastasis. In this study, we investigated the paracrine influence of PVCs derived from human umbilical cords on the proliferation of lung adenocarcinoma epithelial cells (A549) and erythroleukemia cells (TF-$1{\alpha}$ and K562) in vitro using $Transwell^{(R)}$ co-culture systems. PVCs promoted the proliferation of A549 cells without inducing morphological changes, but had no effect on the proliferation of TF-$1{\alpha}$ and K562 cells. To identify the factors secreted from PVCs, conditioned media harvested from PVC cultures were analyzed by antibody arrays. We identified a set of cytokines, including persephin (PSPN), a neurotrophic factor, and a key regulator of oral squamous cell carcinoma progression. Supplementation with PSPN significantly increased the proliferation of A549 cells. These results suggested that PVCs produced a differential effect on the proliferation of cancer cells in a cell-type dependent manner. Further, secretome analyses of PVCs and the elucidation of the molecular mechanisms could facilitate the discovery of therapeutic target(s) for lung cancer.

• Journal of Nutrition and Health
• /
• 제4권4호
• /
• pp.47-56
• /
• 1971

The incidence of diabetes mellitus among Koreans as shown an increasing tendency recently, probably due to the various factors such as the improving living conditions. The majority of people are ignorant or indifferent to the nature, progress and prognosis of diabetes mellitus in Korea. 107 cases of diabetes mellitus which had been admitted to Severance Hospital between January and August, 1971 were Studied. Of these 107 cases, 22 cases were interviewed thoroughly during their hospital stay and the response to their diet therapy was carefully checked. 1) Of the 107 cases 69 cases were male and 38 cases were female; the sex ratio was 18:1. The age of the onset of the disease was as follows: 2 cases were under 20 years of age; 20 cases (18.7%) were under 40 years of age and 85 cases (79.5%) were over 40 years of age. Juvenile diabetes was less frequent when compared with developed countries. 2) Patients complaints and symptoms on admission, complications of sickness, and duration of sickness until the female discharge were also studied. We found that the incidence of tuberculosis complication in diabetes melltius was alarming (13. 8%). 3) In most cases, the control of diabetes was inadequate and diet practisis by the patients was also very poor even when they had known of the diabetes mellitus for a considerable period of time. During hospitalization 75 cases (70.0%) were controlled by diet and oral medications alone. Only 16 cases (15.0%) needed insulin injection, the remaining 16 cases required both diet control and insulin injection. 4) In general, patients received hospital diet satisfactorily. Only a few cases complained of difficulties with milk intake because of no previous dietary experience or of excessive meat orfish because they preferred vegetables and fruit. 5) Patients responded well to the dietitians interviews in the hospital but follow up study and care were poorly organized after discharge from the hospital. 6) The diet exchange 1ist published by the Korean Diabetic Association was not well received by the patients or the general puplic because it is not inexpensive and detailed instructions were not given at the time of discharge from the hospital.

• 대한간호학회지
• /
• 제28권3호
• /
• pp.786-800
• /
• 1998

The purposes of this study were to describe systemically 18 years of oncology nursing research in Korea and suggest it's direction in future. 149 nursing studies published from 1980 to 1998 were selected for the present study. There were examined the source and the design of study, type of subjects, measurement variables, the intervention outcome of experimental research, and theme of qualitative research. The results were as follows : 1. 121 of 149 studies were composed of master thesis and dissertation of graduate school. There were 55 correlations, 30 descriptions, 19 comparisons, 19 qualitative studies and 2 Q-methods as the type of research design. 2. Cancer patients without describing specified diagnosis as subjects' characteristic were 44 of total studies. The others had various diagnoses such as gastric cancer, uterine cancer, breast cancer, leukemia, lymphoma, colorectal cancer, and lung cancer. According to treatment type, patients receiving chemotherapy were the highest number distribution as 53 of all researches. 3. Most measurement instruments used for research were translated it into Korean that developed by foreigners, such as Zung's depression, Spielberg's anxiety, and Wallston's locus of control. 4. Quality of life was shown the most frequently among correlational researches. the next one was depression, the third was hope, and so on. 5. There was the most frequent comparison between cancer and non-cancer patients in comparative researches. It was surveyed those variables as diet habits, risk factors, stressful life events, anxiety and depression, and self-care capacity between two groups. 6. The subjects were mostly chemotherapy patients as 15 of 24 experimental studies. Oral care and education were respectively the highest experimental interventions. 7. Qualitative researches about cancer were reported since 1991. Their themes were illness experience, adaptation process, dying experience, family experience, hope, caring, experience of health behavior, meaning of chemotherapy and experience of cancer survivor. Phenomenologic methodology was designed above 50% of qualitative researches. According to the above findings, cancer research had increased since 1990 and done mostly by descriptive design but a few experimental studies. As recommendations for the future, It is necessary to study the comparison of oncology nursing research internationally, the replication to establish the effect of nursing intervention, and the family care of cancer patient.

• 대한통합의학회지
• /
• 제7권4호
• /
• pp.61-69
• /
• 2019

Purpose : Human gingival fibroblast cell is one of the the main cell types in periodontal tissue, which they can show anti-inflammatory activity through the production of numerous lines of inflammatory mediators such as inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 and interleukins. Porphyromonas gingivalis, one of the oral pathogens, has reported to play a critical role in the development of periodontal diseases. This study aimed to investigate anti-inflammatory and antioxidative activities of Gracilaria textorii ethanol extract (GTEE) in P. gingivalis derived lipopolysaccharide (LPS-PG) stimulated human gingival fibroblast (HGF)-1 cell line. Methods : In order to analyze anti-inflammatory and antioxidative activities of GTEE in HGF-1 cell line, NOS enzyme activity, expression levels of iNOS, COX-2, NAD(P)H quinone dehydrogenase (NQO)1 and their transcription factors were estimated by Griess reaction and western hybridization. Results : LPS-PG induced overexpression of iNOS and COX-2, which was significantly attenuated by GTEE treatment in a dose-dependent manner without any cytotoxicity. In addition, intracellular NOS activity was in accordance with the result of iNOS expression. Due to important role in the regulation of inflammatory responses, phosphorylated status of p65 and c-jun, each subunit of nuclear factor (NF)-κB and activator protein (AP)-1, was also dose-dependently ameliorated by GTEE treatment. One of phase II enzymes, NQO1, and its transcription factor, nuclear factor erythroid 2-related factor 2 (Nrf2), were analyzed since elevated phase II enzyme expression inhibited inflammatory response, which was significantly elevated by GTEE treatment in HGF-1 cell line. Conclusion : In conclusion, GTEE mitigated LPS-PG-stimulated inflammatory responses by attenuating NF-κB and AP-1 activation as well as accelerating NQO1 and Nrf2 expression in HGF-1 cell line. These results indicate that GTEE might be utilized a promising strategy for potential anti-inflammatory agent in periodontal diseases.

• Asian-Australasian Journal of Animal Sciences
• /
• 제33권8호
• /
• pp.1352-1359
• /
• 2020

Objective: Lactobacilli in rabbit intestine is rare and its function in rabbit gut health is not fully understood. The present study aimed to evaluate in vivo the probiotic potential of Lactobacillus casei for suckling rabbits. Methods: Two healthy 5-day-old suckling rabbits with similar weights from each of 12 New Zealand White litters were selected and disturbed to control group and treatment group. All rabbits were artificially fed. The treatment group had been supplemented with live Lactobacillus casei in the milk from the beginning of the trial to 13 days of age. At 15 days of age, healthy paired rabbits were slaughtered to collect intestinal samples. Results: i) Oral administration of Lactobacillus casei significantly increased the proportion of Lactobacilli in the total intestinal bacteria (p<0.01) and obviously reduced that of Escherichia-Shigella (p<0.01); ii) treatment increased the length of vermiform appendix (p<0.05); iii) a higher percentage of degranulated paneth cells was observed in the duodenum and jejunum when rabbits administered with Lactobacillus casei (p<0.01); and iv) the expression of toll-like receptor 9, lysozyme (LYZ), and defensin-7-like (DEFEN) in the duodenum and jejunum was stimulated by supplemented Lactobacillus casei (p<0.05). Conclusion: Orally administered Lactobacillus casei could increase the abundance of intestinal Lactobacilli, decrease the relative abundance of intestinal Escherichia-Shigella, promote the growth of appendix vermiform, stimulate the degranulation of paneth cells and induce the expression of DEFEN and LYS. The results of the present study implied that Lactobacillus casei exhibited probiotic potential for suckling rabbits.

• Environmental Analysis Health and Toxicology
• /
• 제20권1호
• /
• pp.29-37
• /
• 2005

Recently, significant contamination problems by residual chemicals have occasionally been occurred from major rivers and drinking water in Korea. Therefore, the management for use of them and risk assessment should be more strictly performed. In this study, we have analyzed trihalomethanes in treated water samples taken from water plants located in the region of four major rivers (i.e. Han river, Geum river, Youngsan river and Nakdong river) in Korea for eight years (1997～2004). From the data, we could assess the excess cancer risk by calculating the chronic daily intakes (CDI) multiplied by individual oral slope factors, Q₁＊, for the cancer suspected matters such as trihalomethanes, moreover the hazard index which is calculated by dividing the CDI by the acceptable daily reference dose (R/sub f/D) was determined for the risk assessment. As a result, in the case of 95 percentile excess cancer risk, it was shown that the excess cancer risk for dichlorobromomethane in the Nakdong river region is highest among the tested samples as 8.73 x 10/sup -6/. The 95 percentile total hazard index (the sum of individual hazard indices considering R/sub f/D), in addition, was below 1.0 for all samples, and therefore it was assessed that water samples taken from treatment plants of four major rivers are not harmful.

• 대한치과의사협회지
• /
• 제51권7호
• /
• pp.405-413
• /
• 2013

Pediatric and adolescent dental anxiety and fear have been researched in the connection with behavior modification for a long time and this dental anxiety can persist until the adult hoods resulting in extreme fear of and avoidance of dental treatments and causes oral health deterioration. However, the domestic researches regarding adolescent dental anxiety and fear are insufficient. For this reason, this study aims to examine the level of fear and the influential factors affecting their dental fear and to utilize the result as the basic materials in the dental management. The subjects were the students from a middle school located in Gyeonggi-Do. The school was selected by convenient sampling and self-answered questionnaire was filled in by the students. The distribution of responses in each category of fear was examined by frequency analysis. The analysis was conducted using cross tabulation in order to examine the difference between male students and female students and using independent sample t-test in order to compare the fear score of each category. The study results showed the fear score of female students for the category 'causing irritation during the dental treatments' was significantly higher in statistics(p<0.05). Therefore, these results should be reflected to the dental care environments in order for the adolescent to reduce the dental anxiety and fear.

• 대한약침학회지
• /
• 제23권3호
• /
• pp.165-172
• /
• 2020

Objectives: In the past few years, herbal medicines have gained popularity over synthetic drugs because of their natural source and minimal side effects which has led to a tremendous growth of phytopharmaceuticals usage. With the development of nanotechnology, it provides alternative approaches to overcome several limitations using nano-formulations. In spite of considerable quantity of antianemic preparations with different iron forms available, currently additives are used and represented in modern pharmaceutical market. Iron deficiency anemia is a major global public health problem which particularly affects pregnant women, children and elderly persons. The situation is complicated because of disadvantages and drug side effects from existing antianemic medicines. There is a great demand for the development of new antianemic preparations. Green synthesis of iron oxide nanoparticles, possess high potential in this field. Methods: Our study focuses on developing green synthesis of iron oxide nanoparticles (IONPs) of 10-50 nm with spherical shape where different dosages were used -1 mg/kg, 10 mg/kg and 100 mg/kg for exposure in Wistar albino female rats for 28 days. The toxicity was assessed using various parameters such as measurements of the rat body and organ mass, hematology, biochemical evaluation and histopathological examinations. Results: No significant differences were observed in body and organ weights. Hematological indices also indicated no significant differences whereas biochemical factors showed increase in levels of direct bilirubin and globulin of medium as well as high dose and SGPT levels were increased only in high dose. The major organs (heart, kidney and liver) showed histopathological alterations in 10 and 100 mg/kg whereas brain showed only in 100 mg/kg. Conclusion: The toxicity of IONPs was found to be more significant when the concentration was increased; however, low doses can be used for further investigation as an antianemic preparation.

• Journal of Nutrition and Health
• /
• 제54권5호
• /
• pp.474-488
• /
• 2021

Purpose: Malnutrition in children with cerebral palsy (CP) is a significant factor affecting their adequate growth and development. This study aimed at conducting surveys and evaluating the dietary intake of children with CP according to their BMI classification and to thereby highlight the dietary factors affecting the nutritional status of these children. Methods: A total of 16 children were enrolled between the age of four and twelve. These subjects were further classified into three groups, namely underweight, normal and obese, with 6, 8, and 2 children in each group, respectively. The general characteristics, motor disturbances, body composition, feeding problems, eating habits, nutritional intake, dietary variety, and food frequency for children with CP were evaluated. Results: It was observed that motor disturbances tended to increase in underweight children with CP. A significant decrease (p < 0.05) in disturbances related to oral feeding was observed with an increase in obesity. The pattern of eating habits revealed that subjects in the underweight group consumed unbalanced meals, while those in the obese group tended to consume larger meals at a faster pace. The feeding disturbance data revealed that those in the underweight group could not prepare their meals while the obese group had the problem of overeating and consuming an unbalanced diet (p < 0.05). Conclusion: It is necessary for both children with CP, who have a high degree of disability, and their caregivers to take lessons on adequate nutrient intake to prevent malnutrition. Moreover, it is necessary for the caregivers and children with CP having a low degree of disability to take lessons on providing and consuming a balanced diet and to focus on the intake of sufficient calcium in order to prevent obesity.

• Journal of Industrial and Engineering Chemistry
• /
• 제67권
• /
• pp.365-372
• /
• 2018

Wounds that heal with excessive scar formation result in poor functional and aesthetic outcomes. To address this, in our study, visible light cured glycol chitosan (GCH) hydrogels containing endothelial growth factor (EGF) and basic fibroblast growth factor (bFGF) were prepared (GCH-EGF, GCH-FGF and GCH-EGF/FGF) and evaluated their efficacies on the improvement of wound healing in vivo. In vitro release test showed that the growth factors were released in a sustained manner along with initial burst for 24 h. In vitro cell proliferation assay of L-929 mouse fibroblast cell line resulted in the superior ability of GCH-EGF/FGF on the rate. In vivo results demonstrated that the growth factor loaded GCHs further enhanced wound healing compared with GCH. In particular, GCH-EGF/EFG showed the most remarkable wound healing effect among the samples.