• Proceedings of the Korean Society of Toxicology Conference
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• 2002.11b
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• pp.157-157
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• 2002

The toxicity of GX-12, a naked DNA vaccine developed by research team of Dong-A Pharmaceutical Company, Green Cross Company and Genexine for the treatment of HIV infection, was investigated in Sprague-Dawley rats. In single-dose intramuscular/oral acute toxicity studies, animals were treated 0, 250, 1000 or 4000 $\mu\textrm{g}$/kg/$m\ell$ in sodium phosphate buffer.(omitted)

• Journal of the korean academy of Pediatric Dentistry
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• v.33 no.3
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• pp.401-410
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• 2006

Dental caries results from localized demineralization of tooth enamel by acids of bacterial origin produced from the fermentation of dietary sugars. A group of related oral bacteria, collectively known as mutans streptococci, are implicated as the primary etiological agents of human caries. Within this group, Streptococcus mutans has been known as a causative agent for dental caries. As well as acid production yielding the demineralization of tooth enamel, adherence and colonization of S. mutans to the teeth are also important for their virulence Cell-surface fibrillar proteins, which mediate adherence to the salivary pellicle are virulence components of mutans streptococci, and primary candidates for a human caries vaccine. Here we report that the AgI/II gene from S. mutans GS-5 were cloned by PCR amplification of the bacterial chromosomal DNA and the integrity of cloned genes were confirmed by nucleotide sequencing. Sequence analyses showed the sequence alignment of 280 nucleotides between the cloned AgI/II and the reported sequence of S. mutans GS-5 showed the perfect match The cloned genes which signal nucleotide was truncated, were transferred into bacterial expression vector and the recombinant proteins were purified as His-tag fusion proteins In order to generate polyclonal antibodies against the recombinant proteins, AgI/II mr, some $100{\mu}g$ of the proteins was injected into mice three times. It can be used for an effective vaccine production to prevent dental caries caused by pathogenic S. mutans.

• Journal of Oral Medicine and Pain
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• v.45 no.3
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• pp.79-82
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• 2020

Tetanus is a fatal disease caused by the infection Clostridium tetani found in animal feces and soil. It is a vaccine-preventable disease and rarely occurs in developed countries. However, approximately 30 cases still occur annually in South Korea. Tetanus, commonly called lockjaw, cause contraction of the masseter muscles in the early stage, resulting in trismus as the first symptom. As it progresses, spasm extends to various muscles in the face, neck, shoulder, and back, leading to distorted facial expression, dysphagia, backward arching of the body, dyspnea, and even death. Early diagnosis of tetanus is critical because it can quickly become fatal if left untreated. We present a case of trismus caused by tetanus and emphasize the importance of early diagnosis of acute trismus.

• Korean Journal of Veterinary Research
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• v.53 no.3
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• pp.163-167
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• 2013

Avian pathogenic Escherichia coli (APEC) are known to cause extraintestinal disease in poultry, leading to substantial losses in the industry. IutA, iron-regulated aerobactin receptor is firmly associated with APEC. To assess the potential of IutA to induce protective immune responses, attenuated Salmonella Typhimurium strain expressing IutA was constructed and administered orally to BALB/c mice. The IutA-specific immune responses were measured with sera, vaginal and fecal samples by an enzyme-linked immunosorbent assay. We found that the Salmonella-IutA vaccine induced significantly higher immune responses as compared to the control inoculated with the attenuated S. Typhimurium containing the plasmid only. The IutA-specific immune responses were increased by second immunization at third week after initial immunization, whereas triple immunization induced lower immune responses than those induced by the double immunization. The Salmonella-IutA vaccine induced a nature of immunity biased to the Th1-type, as judged by the ratio of IutA-specific IgG isotypes (IgG2a/IgG1). Overall, these results suggest that the Salmonella-IutA vaccine appear to be suitable candidate for a vaccine against APEC.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.110-110
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• 2003

• Proceedings of the Korean Society of Crop Science Conference
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• 2003.10a
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• pp.256-257
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• 2003

• Proceedings of the PSK Conference
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• 2000.04a
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• pp.214.2-215
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• 2000

• Proceedings of the Korean Society of Crop Science Conference
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• 2006.04a
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• pp.184-185
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• 2006

• Proceedings of the Korean Society of Crop Science Conference
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• 2007.04a
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• pp.77.1-77.1
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• 2007

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2002.04b
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• pp.170-170
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• 2002