• The Korean Journal of Mycology
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• v.9 no.3
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• pp.133-139
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• 1981

Two hundred forty seven isolates obtained from 21 white or cream-colored mushroom strain by single spore isolation or multiple spore germination were compared type of mycelial growth in vitro and yield trial in a preliminary test. As a result of these tests, four isolates were selected and compared the yields of sporophores with those of 505 and 702 which are leading strains in mushroom production. The newly selected isolate No. 705 showed high yield of mushroom with good quality as described below. 1. The isolate No. 705 produced 13% more mushrooms than those from the strain No. 703. Both produced creamy type of mushroom. The isolate No. 705 showed high blanching yield ratio and moderate resistance to Mycogone perniciosa. 2. For the isolate No. 705 obtained by multiple spore germinations, the optimum temperature of mycelial growth was $25^{\circ}C$, also the mycelial growth was better at $15^{\circ}C$ than others, optimum moisture content of the compost was 65% and optimum casing soil pH for mycelial growth was 7.8. 3. The new isolate No. 705 produced more number of sporophores and the ratio between parts of sporophores were intermediate of those from the strains No. 505 and No. 703.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.240-240
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• 2017

Quinoa (Chenopodium quinoa Willd.) is one of the ancient crops cultivated in the Andes region at an altitude of 3,500-4000m in Chile and Bolivia from 5000 BC. It contains a large amount of protein, minerals and vitamins in comparison with other crops. The cultivation area has been increasing worldwide because of its excellent resistance to various abiotic stress such as salinity, drought and low temperature. ${\gamma}$-Ray radiation of high dose is often used as a tool to induce mutations in plant breeding, but it has a deleterious effect on organisms. However, the radiation may have a positive stimulatory effect of 'hormesis' in the low dose range. This experiment was carried out to investigate the optimum dose range for creating the quinoa genetic resources and to investigate the hormesis effect at low dose on the quinoa. This experiment was performed for 120 days from November, 2016 to February, 2017 in the greenhouse of Gyeongsang National University. ${\gamma}$-Ray radiation was irradiated to seeds at 0 Gy, 50 Gy, 100 Gy, 200 Gy, 300 Gy, 400 Gy, 600 Gy, 800 Gy and 1000 Gy for 8 hours. (50 Gy) using the low level radiation facility ($Co^{60}$) of Cooperative Research Institute of Radiation Research Institute, KAERI. Fifty seeds were placed on each petri dish lined with wet filter paper and germination rate was measured at a time interval of 2 hours for 40 hrs. The length of the root length was measured one week after germination. Each treatment was carried out in 3 replicates. The growth of seedlings were investigated for 10 days after transplanting of 30 day-old seedlings. The plant height, NDVI, SPAD, Fv/Fm, and panicle weight were measured. The germination rate was highest at 50Gy and 0Gy and the rate of seeds treated with 400Gy or higher rate decreased to 25% of the seeds treated with 50Gy. The emergence rate of seedling in pot experiment was higher at the dose of 200 Gy, 300 Gy and 400 Gy than at 0 and 50Gy. However, the rate was lower at strong radiation higher than 600Gy at which $1^{st}$ leaf was not expanded fully and dead due to extreme overgrowth at 44 days after treatment (DAT). The highest value of panicle weight was observed at 50Gy (6.15g) and 100Gy (5.57g). On the other hand, the weight at high irradiated dose of 300Gy and 400Gy was decreased by about 55% compared to low dose (50 Gy). NDVI measurement also showed the highest value at 50 Gy as the growth progressed. SPAD was the highest at 400 Gy and showed positive correlation with irradiation dose except 0 Gy. Fv/Fm was high at 50 Gy up to 30 DAT and no difference between treatments was observed except for 400 Gy from 44 DAT. The plant height was the highest in 50Gy during the growing period and was higher in the order of 50Dy, 100Gy, 0Gy, 200Gy, 300Gy and 400Gy in 88 DAT. In this experiment, the optimal radiation dose for hormesis was 50Gy and 100Gy, and the optimal radiation dose for mutagenesis seems to be 400 Gy.

• Korean Journal of Plant Resources
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• v.22 no.4
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• pp.304-311
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• 2009

This study was conducted to develop functional sprout vegetables with antioxidant effects using seeds of Arctium lappa. The seeds germinated vigorously under light at $25^{\circ}C$, reaching germination rate of 82% within 4 days. Germinated seeds were placed under darkness at various temperatures to force growth in length, and it was demonatrated that $20^{\circ}C$ was optimum temperature. Greening treatment reduced growth in length, but promoted growth of cotyledons. Harvested A. lappa sprout vegetables maintained freshness longer at $10^{\circ}C$, rather than $4^{\circ}C$. Ventilation holes in storage containers had no effects on storage periods. Antioxidant activity of vegetable that received greening treatment for 1-3 days was investigated, and it was shown that free radical scavenging effects and ferrous ion chelating effects was higher than those of commercially available brocoli, cauliflower, pea and bean sprout. Contents of total polyphenol and flavonoid were also higher, especially by 3 day greening. The longer the treatment, the more the inhibition on peroxidation of linoleic acid. Sprout vegetable of A. lappa had higher antioxidant activity compared with adult plant. In conclusion, sprout vegetable of A. lappa has great potentiality for use as one of sprout vegetables.

• Korean journal of applied entomology
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• v.26 no.4 s.73
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• pp.221-228
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• 1987

The optimum temperature range for conidial germination of Pyriculacia oryzae on a slide glass was $26{\sim}30^{\circ}C$, at which at least four hours of leaf wetness period was required to germinate. Conidial germination was significantly reduced under dry conditions (relative humidity<85%) at $34^{\circ}C$ but not at lower temperature (18, 22, 26, $30^{\circ}C$). Number of lesions developed were greater at $26^{\circ}C$ than at other temperature tested. The average leaf wetness period required for production of a lesion per plant was 22 hours at $18^{\circ}C$, 16 hours at $22^{\circ}C$, 10 hours at $26^{\circ}C$, and 8 hours at $30^{\circ}C$. Less than one lesion per plant occurred at $34^{\circ}C$ even under 24 hours of leaf wetness period. The time period between inoculation and lesion appearance was $7{\sim}8$ days at $18^{\circ}C$, $4{\sim}5$ days at $22^{\circ}C$ and $26^{\circ}C$, and $3{\sim}4$ days at $30^{\circ}C$. The time period required for lesion appearance after inoculation was not affected by leaf wetness period and relative humidity. Lesion length increased most rapidly at $30^{\circ}C$ during the first four days after lesion appearance. Thereater, the rate of increase in lesion length was geratest at $26^{\circ}C$. The average increment of lesion length per day when relative humidity was greater than 90% was 0.7mm at $18^{\circ}C\;and\;22^{\circ}C$, 1mm at $26^{\circ}C$, and 0.8mm at $30^{\circ}C$. When relative humidity was less than 85%, the increments of lesion length per day were approximately $50{\sim}60%$ of those under humid conditions (relative humidity>90%) at all temperature regimes except $30^{\circ}C$. Relative humidity did not significantly affected lesion length at $30^{\circ}C$.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.4
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• pp.484-490
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• 2015

Thirty-eight Pea (Pisum sativum L.) genotypes were screened to identify varieties to be suitable for sprout. Based on seed yield and sprout qualities such as whole length and sprout yield, five genotypes (PI269803, PI343278, PI343283, PI343300 and PI 343307) were primarily selected as candidates for pea sprouts. In order to determine optimal cultivation condition for pea sprouting, growth characteristics were investigated according to the change of germination temperature and days for sprouting. Whole length and hypocotyl length were observed to increase as a time dependent manner at each tested temperature (20, 23, and $25^{\circ}C$). However, whole length, hypocotyl length, and sprout yield were highly increased at $23^{\circ}C$ compared to 20 and $25^{\circ}C$. Especially, PI269803 and PI343300 showed higher sprout yield than the others. In addition, the effect of the change of germination temperature on antioxidant properties was estimated by measuring total phenolic content (TPC) and free radical scavenging activity (DPPH and ABST activity). TPC and DPPH/ABST activities of PI269803 and PI343300 were higher at $23^{\circ}C$ than at 20 and $25^{\circ}C$, while antioxidant properties of PI343278 and PI343283 were decreased in a temperature-dependent manner. The results show a high degree of correlation between TPC and antioxidant activities and suggest that the temperature change for pea sprouting could be responsible for antioxidant properties. Taken together, these results provide optimal cultivation conditions for pea sprouting and suggest that PI269803 and PI343300 with high sprout yield and antioxidant properties could be used for pea sprouts.

• Journal of the Korea Organic Resources Recycling Association
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• v.6 no.2
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• pp.95-112
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• 1998

For the effective disposal of organic food wastes, we seleted 4 strains of microorganism from 186 microbial candidate via enzyme activity test, salt tolerance, food decomposition rate, stability and safety of strains. The identity of these 4 strains are as follows : Fungi is Rhizopus sp., yeasts are Galactomyces sp., Pichia sp. and Hyphopichia sp., In the 50L fermenter scale, we tested various fermenting factor for the optimization of conditions of food waste decomposition using 4 selected strains. The optimum fomenting conditions were as follows : BIO-CHIP Volume 25-30 L, BIO CHIP size 2.0-6.0mm, air flow 200-280L/min, mixing intensity 2-4rpm, temperature $30-45^{\circ}C$. In these fermenting conditions, the efficiency of decomposition(rate of weight loss of food wastes) were 93%. Also the quality of fermenting output were assayed at the basis of fertilizer, and the results were as good as general compost.

• Journal of agriculture & life science
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• v.43 no.5
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• pp.17-25
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• 2009

The purpose of this study was to find out the optimum germination temperature and dehydration period at SMP processing, and applies 3 kind of seed treatment and compares yield with stand establishment of seeds of 12 pepper cultivars. It was showed that all 12 cultivars of nontreated seed does not entirely germinated in $15^{\circ}C$. It was that SMP seed germination rate 77(Yeomyeong)~100% (Deawang) high, and seeds 12 cultivars was tendency high by more than 93% at all temperatures excepting Bugang($35^{\circ}C$, 85%) and Yeomyeong($15^{\circ}C$, 77%). Dehydration condition of result were Gumsure, Dongbang Jonggagip, Hyangchon, Yeomyeong, Kwangbok, Wang by which 3 hours in $45^{\circ}C$ were proper, which 6 hours in $35^{\circ}C$ were proper Manitta, Joyang, and judged that 12 hours in $25^{\circ}C$ were proper in Bugang, Dabotap, Deawang. It was reported that water percentage of moisture content change of pepper seed by dehydration was difference a little in species, but was judged by 3-6 hours in $45^{\circ}C$. There was compared to SMP, Osmopriming, pH control by stand establishment were shown pH control of Hyangchon, Dabotap species was nontreated, osmopriming treatment. Also, Kwangbok species was tendency that stand establishment was low, and Deawang was good than other species. Yield of nontreated of yield day was tendency, and miss planted rate also appeared high for 90 day. There was difference in species, and processing of pH control and osmopriming was not shown stand establishment that is stabilized in all species. SMP processing condition could bring security of stable stand establishment in all species, and was considered that research that was under various environment condition should be proceeded conclusively.

• The Korean Journal of Mycology
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• v.9 no.1
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• pp.31-37
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• 1981

Mycelial growth and fruit body formation of Diehlomyces microsporus were best on mushroom spawn extract medium and rice bran extract medium, respectively. L-asparagine, fructose and glucose were good nutrient sources for mycelial growth. Optimum temperature for mycelial growth ranged at $25{\sim}28^{\circ}C$. Maximum mycelial growth occurred at pH 5.5 while optimum pH for ascospore germination was 6.0. Mycelial mats of D. microsporus did not survive at $60^{\circ}C$ for 60 minutes while ascospores at $80^{\circ}C$ for 120 minutes. Damages of fruit body of Agaricus bisporus caused by D. microsporus were maximum when the fruit bodies were infected at spawning and casing on the compost. The truffle disease could be controlled by basamid with $100{\sim}150 ppm$ treating on the compost after filling.

• Research in Plant Disease
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• v.12 no.1
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• pp.10-14
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• 2006

This study was carried out to know damages of the grapevine trees by the disease to the leaf spot from 2000 to 2003. The isolates collected from different varieties and locations were identified as Pseudocercospora vitis ($(L\acute{e}v)$.) Speg. based on the morphological and cultural characteristics. According to the in vitro test, the range of temperatures for the mycerial growth and the conidical germination of the fungus were from $10^{\circ}C\;to\;35^{\circ}C$ and optimum temperature was $25^{\circ}C$. There were remarkably different features between a low infection trees group and high infection trees group in terms of number of leaves per fruit branch, length and diameter of internode and leaf area.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.43-43
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• 2019

Deparia pycnosora (Christ) M. Kato is a fern used as ornamental plant. In addition, it is called "Teol-go-sa-ri" in Korean name. The aim of this study was to develop a practical propagation method of D. pycnosora using tissue culture technique. Prothallus obtained from spore germination was the used as experiment materials. The prothalli (300 mg) used in all experiments were sub-cultured for 8-week intervals. The most suitable media for prothallus propagation were identified by culturing 300 mg of prothalli in $1/4{\times}$, $1/2{\times}$, $1{\times}$, $2{\times}$ MS medium and in Knop medium for 8 weeks. Also, the prothalli were cultured by chopping with a scalpel. In addition, sucrose, activated charcoal, and total nitrogen source were added in different concentrations based on the culture medium selected. Cultures were maintained at a temperature of $25{\pm}1^{\circ}C$, light intensity of $30{\times}1.0{\mu}mol-m-2{\cdot}s-1$, and a photoperiod of 16/8 h (light/dark) in in vitro. The results showed that optimum was achieved prothallus fresh weight and development in $1{\times}$ MS medium. When other components were added to the basic $1{\times}$ MS medium, prothallus propagation was maximized in $1{\times}$ MS medium supplemented with 2% sucrose, 0.2% activated charcoal, and 60 mM total nitrogen. To select a suitable soil mixture for sporophyte formation, 1.0 g of prothallus was blended with distilled water, spread on five combinations of different soil substrates (decomposed granite, horticultural substrates, peat moss, and perlite), and cultivated for 12 weeks. The sporophyte cultures were maintained at a temperature of $25{\pm}1^{\circ}C$, light intensity of $43{\pm}2.0{\mu}mol-m-2{\cdot}s-1$, humidity of $84{\pm}1.4%$, and a photoperiod of 16/8 h (light/dark). As a results, horticultural substrate alone, 2:1 (v:v) mixtures of horticultural substrate and perlite, and 2:1 mixtures of horticultural substrate and decomposed granite induced 208.0, 201.3 and 248.8 sporophytes per pot, respectively. Therefore, this result could provide a practical mass propagation method of D. pycnosora