• Journal of the Korean Graphic Arts Communication Society
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• v.6 no.1
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• pp.59-66
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• 1988

One of the rising problems in the field of environmental engineering is the eutrophic phenomenon which appears in the closed water basin. This study is the elimination orthophosphate by adsorption, which is indispensible nutritive element for eutrophication Ziriconium(IV) oxide hydrate-activatedcarbon complex. which has excellent adsorptivity for orthophosphate and can easily be reused after desorption process has been made and used as adsorbents. The main purpose of this study is to obtain intraparticle diffusivity which is basic data for the design of adsorption operation the experimental results were as follows. 1. Optimum condition which gave maxinum adsorption of orthophosphate was ph5 2. Equilibrium adsorption showed good consistency with Freundlich type. q=10.80C1/2.08 3. Pore diffusivity and surface diffusivity obtained the kinetic experiment of batch adsorption were respectivity 5.38 and 2.06 ($\textrm{cm}^2$/sec)

• Journal of Plant Biotechnology
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• v.6 no.2
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• pp.125-130
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• 2004

Callus and cell suspension cultures of Eurycoma longifolia Jack could be an alternative supply of 9-hydroxycanthin-6-one and 9-methoxycanthin-6-one. The callus tissues were initiated from leaves of different trees. The friable calli were used for the preparation of the cell suspension cultures of E. longifolia. The leaf explant of tree Eu-9 produced the most callus and also induced high cell biomass in the cell suspension culture, but it produced low quantity of 9-methoxycanthin- 6-one and 9-hydroxycanthin-6-one. The leaf explant from tree Eu-8 produced low quantity of callus and cell biomass, but produced the highest quantity of 9-methoxycanthin- 6-one and 9-hydroxycanthin-6-one. Optimum production of cell biomass was obtained on cell culture medium with pH 5.75 prior to autoclaving, but high alkaloids content could be induced in culture medium in acidic condition with pH 4.75 and 5.25 prior to autoclaving.

• Journal of Environmental Health Sciences
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• v.16 no.1
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• pp.21-28
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• 1990

As a research for treatment of waste water by biological method, we investigated general characteristics of waste water and isolated some useful bacteria which effectively treated waste water. Compositions of waste water were analyzed to give COD 2060 ppm, PVA 560 ppm, T-N 50 ppm, T-P 3.3 ppm and PH 12. Also, we inverstigated optimum nutrients requirement and growth conditions by mixed culture as well as the effect of coagulants. The COD removal rate reached maximum state for 48 hrs culture at pH 7.0 and 30$^{\circ}$C. Alum as the coagulated was the most effective. The COD removal rate was also increased by supplementing 10 ppm phosphorous sources as additional nutrients. The COD of waste water was reduced to 10% of its initial value by the continuous culture. As a result of overall experiments the COD of effluents became about 100 ppm and final pH 7.

• Microbiology and Biotechnology Letters
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• v.28 no.5
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• pp.251-257
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• 2000

Serratia marcescens purine nucleoside phosphorylase (PNP) was purfied to homogeneity by streptomycin sulfate treatment, Sephacry HR S-200 gel filtration chromatography and AMP-agarose affinity chromatography. The specific activity of the enzyme was increased 49-fold during purification with an overall yield of 7.0%. The molecular weight was 168kD as estimated by Sephadex G-150 gel filtration chromatography. The S. marcescens enzyme was composed of six identical subunits with subunit molecular weight of 28kD, as estimated by SDS-PAGE. The Km values of S. marcescens enzyme for inosine and deoxyinsoine were 0.38 and 1.20 mM, respectively. The ph optimum was near 8.0, and the enzyme was relatively heat-stable protein. The enzyme was inactivated com-pletely by 0.5 mM of $Cu^{ 2+}$.

• Korean Journal of Hydrosciences
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• v.1
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• pp.99-106
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• 1990

An extensive biological nutrient removal pilot plant study of anoxic/anaerobic/ aerobic treatment process was conducted to eastblish an optimum operational mode using primary dffluent. Two operational modes, (1) Qr/Q was 3.0 and maintaining EMLSS of 3100 mg/L in which the best operational results were obtained from previous bench scale study using synthetic wastewater (2) Qr/Q was 0.5 and EMLSS of 2200 mg/L which was compatible with the main plant, were Compared and evaluated for removal of nitrogen and/or phosphorous under field conditions. The nitrogen removal increased with increasing recycle ratios, but the phosphorous removal revealed more consistent results with 83percent removal efficiency in the second mode compared with 80 percent in the first mode. Above all, the two modes equally showed good BOD and nitrogen removals by nitrification-denitrification processes. It was also observed that no scum formed in the pilot plant and the sludge exhibited excellent settling characteristic all the time. The modified biological nutrient removal train can be adopted to the main plant without any major changes of their operational modes.

• The Korean Journal of Food And Nutrition
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• v.5 no.2
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• pp.77-83
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• 1992

The optimum conditions and mechanisms for the plasmid-mediated genetic transformation of intact cells of Bacillus brevis Pl76-2, an extracellular protein producing bacterium by electroporation were investigated. It was found that pUB110 Plasmid DNA can be introduced into intact bacterial cells by electroporation. The frequency of transformation by this electroporation system depended upon the initial electric field strength, the capacity of the electric discharge capacitor, growth stage, number of successive pulses and composition of electroporation buffer. It was effective for transformation that cells were harvested, washed and resuspended with HSM [7M HEPES(PH 7.4), 272mM sucrose, 1 mM MgCl2] electroporation buffer when cell growth was attained to 1.2 at OD660. A maximum frequency of transformation of 2.40$\times$104 transformants per$\mu$g plasmid DNA was obtained by two succesive Pulses with an initial electric field strength of 12.5kV/cm and with a capacitance of 7.3uF.

• Textile Coloration and Finishing
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• v.18 no.6 s.91
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• pp.1-9
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• 2006

The purpose of this study is to investigate the dyeability, antibacterial activity on silk and rayon fabrics dyed with cochineal. The result are as follows; 1. The K/S value of silk fabric was higher than that of rayon fabric. 2. The optimum conditions are mordant concentration $0.5{\sim}1%$, dyeing material concentration 2.0%(o.w.f), dyeing temperature $60^{\circ}C$, PH 3 and dyeing time 30minutes. 3. Pre-mordanting method had higher dyeing absorption than post mordanting method in case of silk and rayon fabrics. 4. The antibacterial activity of dyed silk fabrics were higher than that of dyed rayon fabrics and the antibacterial activity was increased by mordanting. 5. The lightfastness and perspiration fastness of silk fabric were improved but these of rayon were not. Dyeing fastness was improved by Fe mordanting on both fabrics.

• Korean Journal of Microbiology
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• v.10 no.3
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• pp.106-108
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• 1972

The enzyme was produced by Asp.oryzae SHW 131. the enzymatic properties of .alpha.-amylase are following : 1) Crystallization of .alpha.-amylase is formed of longish square. 2) The range of stable pH is 5-10 and optimum ph is 5.5. 3) It is very unstable enzyme about EDTA and protection by $Ca^{++}$ ion and best activated at $50^{\circ}C$ about temperature. 4) Asp.oryzae SHW 131 produced .alpha.-amylase with acid-protease, neutral-protease and tepid-alkalin-protease.

• Korean Journal of Microbiology
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• v.10 no.3
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• pp.105-105
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• 1972

The enzyme was produced by Asp.oryzae SHW 131. the enzymatic properties of .alpha.-amylase are following : 1) Crystallization of .alpha.-amylase is formed of longish square. 2) The range of stable pH is 5-10 and optimum ph is 5.5. 3) It is very unstable enzyme about EDTA and protection by $Ca^{++}$ ion and best activated at $50^{\circ}C$ about temperature. 4) Asp.oryzae SHW 131 produced .alpha.-amylase with acid-protease, neutral-protease and tepid-alkalin-protease.

• Applied Chemistry for Engineering
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• v.9 no.1
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• pp.58-65
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• 1998

Substituted dicyandiamide(Sub-DICY), Accelerated dicyandiamide(Acc-DICY), Trimellitic anhydride(TMA), Pyromellitic dianhydride(PMDA) and Phenolic curing agent(Ph.C.A.) are mainly used for epoxy powder coating curing agent. Various characteristics of epoxy films fully cured by optimum condition such as mechanical properties like $T_g$, tensile strength, elongation at break hardness, abrasion resistance and chemical properties like water absorption, acid resistance, alkali resistance and electrical properties, corrosion resistance are determined by various measuring devices and analyses devices. In conclusion, phenolic curing agent was shown excellent thoughness but severe color change as temperature increased. Acid anhydride has excellent insulation properties and color stability at elevated temperature but lower thoughness and adhesion to substrate. DICY curing agent was shown high water absorption and severe color chance as temperature increased.