• 한국진공학회지
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• 제18권6호
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• pp.440-446
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• 2009

본 연구에서는 인라인 스퍼터링 공정을 이용하여 저항막 방식 터치패널에 적용이 가능한 인듐 주석 산화물(indium tin oxide: ITO)기판 제조 공정을 최적화하였다. 플라스틱 기판으로 고분자 기판인 폴리카보네이트(polycarbonate : PC) 기판을 사용하였으며 면저항 $500{\pm}50\;{\Omega}$/□, 면저항 균일도 10 % 이내, 투과율 87 %(at550nm)이상, 두께 $120{\sim}250\;{\AA}$의 조건을 만족하는 ITO 기판의 공정 최적화를 위한 실험을 진행하였다. 스퍼터링 공정은 초기 진공도 $1{\times}10^{-6}\;torr$ 일 때 상온에서 DC 전압을 인가하여 진행되었으며 산소/알곤 가스의 비율, 인가 전압, 공정 압력, 기판의 이송 속도를 변수로 하여 요구되는 ITO 박막의 특성에 가장 최적화된 공정 조건을 형성하였다. 인라인 방식의 스퍼터 장비에서 최적화된 공정 조건은 실제 생산 라인에 적용이 가능할 것으로 판단되며 향후 지속적인 연구를 통해 현재 수입에 의존하고 있는 ITO 기판 제조기술의 국산화에 기여할 수 있을 것이다.

• Journal of Microbiology and Biotechnology
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• 제1권2호
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• pp.116-120
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• 1991

The transformant Bacillus subtilis ED213 carrying the pSO100 which cloned the cdd gene encoding cytidine deaminase (cytidine /2'-deoxycytidine aminohydrolase, EC 3.5.4.5, CDase) originated from wild type B. subtilis was cultivated in Spizizen minimal medium (SMM). To overcome poor expression of the cdd gene in SMM medium, the medium compositions and growth conditions were optimized. The optimized medium compositions and growth conditions were cytidine concentration of 80 mg/l, glycerol of 25 g/l, and $(NH_4)_2SO_4$ of 10 g/l, along with $37^{\circ}C$ and pH 7.0. The intracellular CDase production was increased 3 times from 1,000 unit/ml to 3,200 unit/ml, and extracellular CDase also increased from nearly undetectable amounts to 1,500 unit/ml. The cytidine concentration was signified as the most critical compositional factor for overproduction of CDase by increasing the cell density mainly in culture broth. The plasmids were more stable in cells that were grown in original SMM medium with stability of 90% compared to those grown in optimized SMM medium with stability of 80% after 48 hours cultivation. The most active amplification of plasmid was occurred in the logarithmic phase, which showed a value around four times higher than the initial copy number. In the exponential phase, the CDase production was closely related to the plasmid copy number along with the cell density. However, it was not accorded with cell density at the stationary phase.

• Tribology and Lubricants
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• 제30권3호
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• pp.177-182
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• 2014

To stop a high speed train running at the speed of 300 km/h, the disc brake for the train should be able to dissipate enormous kinetic energy of the train into frictional heat energy. Sintered pin-type metals are mostly used for friction materials of high speed brake pads. A pad comprises several friction pins, and the topology, length, flexibility, composition, etc. have a great influence on the tribological properties of the disc brake. In this study, the topology of the friction pins in a pad was our main concern. We presented the optimization of the topology of a railcar brake pad with nine-pin-type friction materials by thermo-mechanical contact analysis. We modeled the brake pad with/without a back plate. To simulate a continuous braking, the pad or friction materials were rotated at constant velocity on the friction surface of the disc. We varied the positions of the nine friction materials to compare the temperature distributions on the disc surface. In a non-optimized brake pad, the distance between two neighboring friction materials in the radial direction from the rotational center of the disc was not equal. In an optimized pad, the distance between two neighboring friction materials in the radial direction was equal. The temperature distribution on the disc surface fluctuated more for the former than the latter. Optimizing the pad reduced the maximum temperature of the brake disc by more than 10%.

• 한국마린엔지니어링학회:학술대회논문집
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• 한국마린엔지니어링학회 2005년도 후기학술대회논문집
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• pp.212-213
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• 2005

The results of an experimental and analytical study of composite pressure hull on buckling pressure are presented for URN 300. We predicted the buckling and post buckling analysis of composite laminated cylindrical shell and panel under external compression by using ABAQUS/Standard[Ver 6.4]. To obtain nonlinear static equilibrium solutions for unstable problems, where the load-displacement response can exhibit the type of nonlinear buckling behavior, during periods of the response, the load and/or the displacement may decrease as the solution evolves, used the modified Riks method. Experiments were conducted to verify the validation of present analysis for cross-ply laminated shells. The shells considered in the study have four different lamination patterns, [${\pm}{\Theta}$/0/90]$_{14s}$,[${\pm}{\Theta}_{14}$/$0_{14}$/$90_{14}$],[${\pm}$45/0/90]$_{18s}$ and [/0/90]$_{18s}$. At the result of this study, the optimized ply orientation angle is $75^{\circ}$. The critical load from experiment is 69% of that of numerical analysis, because the fracture of matrix was generated before buckling. So URN 300 is not proper to use at the condition under high external pressure.

• Biomolecules & Therapeutics
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• 제12권3호
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• pp.179-184
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• 2004

The FimH subunit of type 1-fimbriated Escherichia coli has been determined as a major cause for urinary tract infections. In our previous study, the Adhesin/CTXA2B was expressed as soluble recombinant chimaeric protein derived from the uropathogenic Escherichia coli adhesin genetically coupled to cholera toxin A2B (CTXA2B) subunit in Escherichia coli. Since it is very important to optimize IPTG concentration and culture temperature to maximize cell growth and productivity, These optimal culture factors were determined to increase the productivity of the expressed Adhesin/CTXA2B chimaeric protein in Escherichia coli TB1 carrying pMALfimH/ctxa2b. Our data demonstrate that optimal concentration of IPTG for increased production of chimaeric protein was 0.5 mM. Additionally, culture time was 10 hours and temperature, 37${\circ}C$.

• Journal of Microbiology and Biotechnology
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• 제26권8호
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• pp.1348-1357
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• 2016

An enzymatic reaction system was developed and optimized for bioconversion of resveratrol from glucose. Liquid enzyme extracts were prepared from Alternaria sp. MG1, an endophytic fungus from grape, and used directly or after immobilization with sodium alginate. When the enzyme solution was used, efficient production of resveratrol was found within 120 min in a manner that was pH-, reaction time-, enzyme amount-, substrate type-, and substrate concentration-dependent. After the optimization experiments using the response surface methodology, the highest value of resveratrol production (224.40 μg/l) was found under the conditions of pH 6.84, 0.35 g/l glucose, 0.02 mg/l coenzyme A, and 0.02 mg/l ATP. Immobilized enzyme extracts could keep high production of resveratrol during recycling use for two to five times. The developed system indicated a potential approach to resveratrol biosynthesis independent of plants and fungal cell growth, and provided a possible way to produce resveratrol within 2 h, the shortest period needed for biosynthesis of resveratrol so far.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2005년도 춘계학술대회 논문집 디스플레이 광소자 분야
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• pp.123-126
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• 2005

Eye Glass Display (EGD) with microdisplay to realize the virtual display can make the large screen, so virtual image has been developed by using microdisplay panel. This paper shows study of low cost lens design and simulation for microdisplay system with 0.6"LCoS panel. Lens design optimized consider to spherical aberration, astigmatism, distortion, and chromatic aberration. Code V is used and it designed an aspheric lens about exit pupil 6mm, eye relief 20mm and 35 degree of field of view (FOV). With the application this aspheric lens to liquid crystal on silicon (LCOS) type's microdisplay, virtual image showed 50 inch at 2m. One side of the aspheric lens was constituted from diffractive optical element (DOE) for the improvement in a performance. It had less than 2.5% of distortion value and modulation transfer function in axial had 20% of resolution with 32 lp/mm spatial frequency. The optical system is suitable for display of 15.6 mm-diagonal with SVGA.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
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• pp.308-309
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• 2016

Reducing surface recombination is a critical factor for high efficiency silicon solar cells. The passivation process is for reducing dangling bonds which are carrier. Tunnel oxide layer is one of main issues to achieve a good passivation between silicon wafer and emitter layer. Many research use wet-chemical oxidation or thermally grown which the highest conversion efficiencies have been reported so far. In this study, we deposit ultra-thin tunnel oxide layer by PECVD (Plasma Enhanced Chemical Vapor Deposition) using $N_2O$ plasma. Both side deposit tunnel oxide layer in different RF-power and phosphorus doped a-Si:H layer. After deposit, samples are annealed at $850^{\circ}C$ for 1 hour in $N_2$ gas atmosphere. After annealing, samples are measured lifetime and implied Voc (iVoc) by QSSPC (Quasi-Steady-State Photo Conductance). After measure, samples are annealed at $400^{\circ}C$ for 30 minute in $Ar/H_2$ gas atmosphere and then measure again lifetime and implied VOC. The lifetime is increase after all process also implied VOC. The highest results are lifetime $762{\mu}s$, implied Voc 733 mV at RF-power 200 W. The results of C-V measurement shows that Dit is increase when RF-power increase. Using this optimized tunnel oxide layer is attributed to increase iVoc. As a consequence, the cell efficiency is increased such as tunnel mechanism based solar cell application.

• 한국강구조학회 논문집
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• 제15권4호통권65호
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• pp.389-401
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• 2003

본 연구의 목적은 H형강 단면과 콘크리트의 합성단면으로 구성된 보와 기둥의 합성 뼈대구조물에 대해 신뢰성지수를 고려한 최적 단면을 설계할 수 있는 알고리즘을 개발하는데 있다. 합성 뼈대구조물의 최적화 문제는 단면 치수를 설계변수로 취하고 목적함수와 제약조건을 형성한다. 목적함수는 구조물의 총 경비로 형성하고, 제약조건식은 단면응력과 허용응력의 신뢰성지수를 고려하여 유도한다. 합성 뼈대구조물의 단문을 최적화하는 알고리즘은 수정 Newton-Raphson 탐사법을 사용하는 SUMT기법을 사용한다. 본 연구에서 개발된 최적화 알고리즘은 1층 1경간 합성 뼈대구조물과 5층 1경간 합성 뼈대구조물의 수치예에 신뢰성지수(${\beta}=3.0$, ${\beta}=0.0$)를 고려한 합성 뼈대구조물 설계의 실용화를 위하여 적용된다. 제안된 알고리즘의 최적화 가능성과 적용성 그리고 수렴성 등을 살펴보기 위하여 수치결과들을 비교 분석한다.

• Journal of Microbiology and Biotechnology
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• 제18권7호
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• pp.1317-1325
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• 2008

Viral safety is an important prerequisite for clinical preparations of plasma-derived pharmaceuticals. One potential way to increase the safety of therapeutic biological products is the use of a virus-retentive filter. In order to increase the viral safety of human antihemophilic factor IX, particularly in regard to non-enveloped viruses, a virus removal process using a polyvinylidene fluoride membrane filter (Viresolve NFP) has been optimized. The most critical factor affecting the filtration efficiency was operating pH and the optimum pH was 6 or 7. Flow rate increased with increasing operating pressure and temperature. Recovery yield in the optimized production-scale process was 96%. No substantial changes were observed in the physical and biochemical characteristics of the filtered factor IX in comparison with those before filtration. A 47-mm disk membrane filter was used to simulate the process performance of the production-scale cartridges and to test if it could remove several experimental model viruses for human pathogenic viruses, including human hepatitis A virus (HAV), porcine parvovirus (PPV), murine encephalomyocarditis virus (EMCV), human immunodeficiency virus type 1 (HIV), bovine viral diarrhea virus (BVDV), and bovine herpes virus (BHV). Non-enveloped viruses (HAV, PPV, and EMCV) as well as enveloped viruses (HIV, BVDV, and BHV) were completely removed during filtration. The log reduction factors achieved were $\geq$6.12 for HAV, $\geq$4.28 for PPV, $\geq$5.33 for EMCV, $\geq$5.51 for HIV, $\geq$5.17 for BVDV, and $\geq$5.75 for BHV. These results indicate that the virus filtration process successfully improved the viral safety of factor IX.