• KSBB Journal
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• v.19 no.4
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• pp.295-300
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• 2004

A marine bacterium for producing an collagenolytic protease was isolated from the southern sea of Korea and identified as Vibrio vulnificus and named as Vibrio vulnificus CYK279H. This strain producing an collagenolytic protease was showed high activity toward collagen and gelatin as substrate. The optimum initial pH, NaCl, and temperature for cell growth and protease production was 7.5, 2.0% and 25$^{\circ}C$, respectively. Optimization for collagenolytic protease production was composed of 0.3% D-galactose, 0.6% yeast extract, 4.0% gelatin, 0.2% (NH$_4$)$_2$SO$_4$, and 0.2 mM ferric citrate in artificial sea water. The maximum protease production was required gelatin and yeast extract. The collagenolytic protease production by Vibrio vulnificus CYK279H reached a maximum of 73 unit/l after the cultivation for 18 h under the optimized medium.

• Microbiology and Biotechnology Letters
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• v.9 no.1
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• pp.29-34
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• 1981

To maximize the production of penicillin amidase from Estherichia coli (ATCC 9637), the media composition and several factors affecting the engyme production during fermentation were studied. The optimal media composition was found to be; 3.5% tryptone, 1.5% monosodium glutamate and 0.5% yeast extract. The addition of 0.15% phenylacetic acid as an enzyme inducer at the initial stage of cultivation increased the engyme productivity about 5 fold. It was found that the engyme activity reached maximum within 16hr of cultivation. The maximum production of the enzyme obtained was about 102.5 units/l broth under the optimized condition. The enzyme production was markedly increased by the optimization as compared with those previously reported.

• Microbiology and Biotechnology Letters
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• v.16 no.4
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• pp.327-333
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• 1988

For optimal production of recombinant human interleukin-2 (IL-2) in E. coli the effect of fermentation conditions on cell growth, IL-2 production, and stability of recombinant cells were investigated. Among the complex nutrients tested in this work, yeast extract, peptone and corn steep liquor were found to be effective for recombinant cell growth. The recombinant cells were maintained stably under repression condition (3$0^{\circ}C$), but the stability of recombinant cells were drastically reduced upon induction of IL-2 expression (42$^{\circ}C$) even under the selection pressure. Addition of antibiotics to the culture medium resulted in the cell growth inhibition without significant improvement in recombinant stability. When the expression of IL-2 gene was induced at different growth phases, highest IL-2 production was achieved by the induction of IL-2 at the middle-exponential growth phase. It was found that the production of IL-2 significantly inhibited the cell growth and the ex-pression of other genes in the plasmid.

• Journal of the Korean Applied Science and Technology
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• v.25 no.3
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• pp.299-312
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• 2008

The fat of Tra and Basa catfish (Mekong Delta, Viet Nam) was evaluated for the first time as the potential feedstock for biodiesel production, due to its abundance, availability and cheap cost. The unsaturated fatty acid contents of Tra and Basa fat were 57.97% and 64.17%, respectively. Biodiesel was prepared from Tra and Basa fat by methanolysis reaction using alkali catalysts like sodium hydroxide and potassium hydroxide. Effects of various process parameters on biodiesel production, such as molar ratio of methanol to fat, catalyst concentration, temperature and time were investigated. As those results, the transesterification can be performed under moderate conditions, and the biodiesel yields were shown more than 90%. KOH catalyst was the best catalyst for biodiesel production from both Basa and Tra fat. As the feedstock aspect, Basa fat was indicated more efficiency than that of Tra fat. The maximum yield could be achieved by the transesterification from Basa fat with 5:1 molar ratio of methanol to fat, 0.8% KOH catalyst, $50^{\circ}C$, and 50 min. For Tra fat, the optimal condition were at 6:1 molar ratio of methanol to fat, 0.8% KOH catalyst, $50^{\circ}C$, and 45 min. Nowadays, due to cheaper cost and abundance, Tra fat is a promised resource for cheap biodiesel production in Viet Nam.

• Korean Journal of Agricultural Science
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• v.32 no.1
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• pp.81-94
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• 2005

This study is aimed at giving the basic information for individual farm households to make decisions for optimizing their farm sizes and for the government to implement farm size optimization policies through the identification of combinations among rice production factors in plain areas like Cheolwon district and the suggestion of the optimal farm sizes of individual farmers based on the scale of economy calculated. The data of agricultural production costs of 50 rice farmers in the plain area which is located in Dongsong-eup Cholwon district, Kangwon province were used in the analysis. The 'translog' cost function among various methods which is a flexible function type was adopted to calculate the scale of economy in rice production. Seemingly unrelated regression(SUR) method was used in forecasting functions and processing other statistics by SHAZAM which is one of the computer aid program for quantitative econometric analysis. In conclusion, the long-run average cost(LAC) curve showed 'U-shape' which was different from 'L-type' one which was shown in the previous studies by others. The lowest point of the LAC was 9.764ha and the concerned production cost amounted to 633 Won/kg. Based on these results, it have to be suggested that around 10 ha of paddy is the target size for policy assistances to save costs under the present level of farming practices and technology. The above results show that the rice production costs could be saved up to 10ha in Cheolwon plain area which is a typical paddy field. However, land use, land condition, land ownership and manager's ability which may affect scale of economy should be considered. Furthermore, reasonable management will have to be realized by means of labor saving technology and cost saving management skill like enlargement of farm size of rice.

• Microbiology and Biotechnology Letters
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• v.28 no.6
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• pp.329-333
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• 2000

Alkaline bacterium producing a high pro-tease activity at low temperature was isolated by using enrichment culture from soil samples and identified as Bacil-lus sp. WRD-1 Cell growth was maximal at 10 hours and the optimal initial pH and culture time of culture condition for enzyme production was pH 7 and 10 hours, respectively. Temperature range of high enzyme activity were $10~40^{\circ}C$. The optimal pH and temperature for the enzyme activity were pH9 and $30^{\circ}C$.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.7
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• pp.1053-1060
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• 2015

In this study, the optimal operation factors for struvite crystallization for removing and recovering nitrogen and phosphorus from anaerobic digestive fluid of swine manure containing highly concentrated nitrogen was determined. Every experiment for the struvite crystallization reaction was conducted by placing 1,000 mL of digestion fluid in a 2,000 mL Erlenmeyer flask at various temperatures, pH, and mixing speed. Except for special circumstances, the digestion fluid was centrifuged (10,000 rpm, 10 min) and then the supernatant was used for the experiment at room temperature and 100 rpm. The optimal mole ratio of $PO_4{^{3-}}:Mg^{2+}$ was 1:1.5, and the pH effect ranging from 9 to 11 was similar, when mixed for 1 hour. Under this condition, the removal efficiency of $NH_4{^+}-N$ and $PO_4{^{3-}}-P$ was 40% and 88.6%, respectively. X-shaped crystal was observed by light and scanning electron microscopy. In addition, struvite crystal structure was confirmed through X-ray diffraction analysis.

• Ocean Systems Engineering
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• v.8 no.3
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• pp.247-279
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• 2018

This study aims to investigate the behavioral characteristics of the LWSCR (lazy-wave steel catenary riser) for a turret-moored FPSO (Floating Production Storage Offloading) by using fully-coupled hull-mooring-riser dynamic simulation program in time domain. In particular, the effects of initial geometric profile on the global performance and structural behavior are investigated in depth to have an insight for optimal design. In this regard, a systematic parametric study with varying the initial curvature of sag and arch bend and initial position of touch down point (TDP) is conducted for 100-yr wind-wave-current (WWC) hurricane condition. The FPSO motions, riser dynamics, constituent structural stress results, accumulated fatigue damage of the LWSCR are presented and analyzed to draw a general trend of the relationship between the LWSCR geometric parameters and the resulting dynamic/structural performance. According to this study, the initial curvature of the sag and arch bend plays an important role in absorbing transferred platform motions, while the position of TDP mainly affects the change of static-stress level.

• Journal of the Korean Society for Precision Engineering
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• v.25 no.4
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• pp.108-117
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• 2008

A transmission designed with P.T.O(Power Take-Off) axle for agricultural work vehicles including multi-purpose vehicles has been developed. It is focused on the 4-wheel drive transmission of synchronous contact type for practical use in fruit tree households which is required for a large-sized agricultural vehicle. Concerning to the specification performed, the load capacity is from 500kg to 1,000kg and the safety should be secured for passengers. In addition, the driving condition should also be secured under bad situations of the topographic slope, swampy land and the rest. In order to carry out above tests, a prototype vehicle through strength analysis of transmission design has been manufactured. Consequently, optimal design conditions on the power transmission with multi-purpose vehicle for various jobs are proposed such as an indication of optimal RPM and torque at a certain work situation. The performance test through the prototype of multi-purpose work vehicle is performed and the related data base is achieved. Finally, it is improved on troubles by the analysis of the results of R&D and provided the solutions on problems occurring to mass production in the future.

• Food Science and Biotechnology
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• v.14 no.5
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• pp.651-655
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• 2005

Enzymatic hydrolysis of shark (Squatina oculata) cartilage (SC) was optimized by response surface methodology (RSM) for chondroitin sulfate (CS) preparation. Among 11 commercial proteases, Maxazyme NNP showed highest productivity (CS yield per enzyme cost) of CS. Optimal hydrolysis conditions determined by RSM were 1.63% and 2.87 hr for enzyme concentration and hydrolysis time ($r^2\;=\;0.9527$, p<0.0l), respectively and highest yield of hydrolysate under the conditions was 42.3%. The yield ($43.1{\pm}2.1%$) and CS content ($24.8{\pm}0.1%$) of hydrolysate at optimal condition verified statistical optimization of SC enzymatic hydrolysis was valid.