• Title/Summary/Keyword: Optimal growth temperature

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Screening of Microorganisms Secreted High Efficient Enzymes and Properties of Enzymatic Deinking for Old Newsprint(II) - Isolation and screening of fungi producing cellulase and xylanase- (고효율 효소를 분비하는 균주의 선발 및 신문고지의 효소탈묵 특성(제2보) -Cellulase와 Xylanase를 생산하는 Fungi의 분리 및 선발-)

  • Park Seong-Cheol;Kang Jin-Ha;Lee Yang-Soo
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.36 no.3
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    • pp.9-14
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    • 2004
  • The useful fungi which secret extracellular enzymes was selected for deinking agent of old newsprint. Five fungal strains were isolated from a paper mill soil ground. The CMCase, FPase and xylanase activities of fungi on the liquid culture were investigated at optimal growth conditions. The results of this study were as follow: The optimal pH and temperature for culture growth were 4~8 and 27~$35^{\circ}C$, respectively. For screening of extracellular enzymes at optimal culture conditions the optimal culture period were less than 6-7 days. Fusarium pallidoroseum and Aspergiilus niger which shows relatively higher CMCase, FPase and xylanase activities than the other species were selected for further enzymatic deinking research.

Optimization of Growth Environments Based on Meteorological and Environmental Sensor Data (기상 및 환경 센서 데이터 기반 생육 환경 최적화 연구)

  • Sook Lye Jeon;Jinheung Lee;Sung Eok Kim;Jeonghwan Park
    • Journal of Sensor Science and Technology
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    • v.33 no.4
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    • pp.230-236
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    • 2024
  • This study aimed to analyze the environmental factors affecting tomato growth by examining the correlation between weather and growth environment sensor data from P Smart Farm located in Gwangseok-myeon, Nonsan-si, Chungcheongnam-do. Key environmental variables such as the temperature, humidity, sunlight hours, solar radiation, and daily light integral (DLI) significantly affect tomato growth. The optimal temperature and DLI conditions play crucial roles in enhancing tomato growth and the photosynthetic efficiency. In this study, we developed a model to correct and predict the time-series variations in internal environmental sensor data using external weather sensor data. A linear regression analysis model was employed to estimate the external temperature variations and internal DLI values of P Smart Farm. Then, regression equations were derived based on these data. The analysis verified that the estimated variations in external temperature and internal DLI are explained effectively by the regression models. In this research, we analyzed and monitored smart-farm growth environment data based on weather sensor data. Thereby, we obtained an optimized model for the temperature and light conditions crucial for tomato growth. Additionally, the study emphasizes the importance of sensor-based data analysis in dynamically adjusting the tomato growth environment according to the variations in weather and growth conditions. The observations of this study indicate that analytical solutions using public weather data can provide data-driven operational experiences and productivity improvements for small- and medium-sized facility farms that cannot afford expensive sensors.

Lead Tolerance Profile of Pseudomonas Stuzeri in Liquid Culture

  • Kim, Su-Jung;Jung, A-Young;Joo, Jin-Ho
    • Korean Journal of Environmental Agriculture
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    • v.26 no.4
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    • pp.332-336
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    • 2007
  • Pseudomonas stutzeri strain KCCM 34719 was used in this experiment to determine the effects of increasing Pb(II) concentrations on its growth rate. To obtain optimum growth conditions, strain KCCM 34719 was cultivated in nutrient broth under various conditions, such as temperature, pH, and NaCl concentration. Optimal conditions for cell growth were $30^{\circ}C$ of temperature, 8.0 of pH, and 3% of NaCl concentration, respectively. Growth response of bacterial cell to Pb(II) showed tolerance to concentrations ranging from 10 to 100 mg ${\ell}^{-1}$ in liquid culture, following a growth pattern similar to the control. Growth rate was greatly inhibited at 200 mg ${\ell}^{-1}$ of Pb(II).

A Study on High Temperature Low Cycle Fatigue Crack Growth Modelling by Neural Networks (신경회로망을 이용한 고온 저사이클 피로균열성장 모델링에 관한 연구)

  • Ju, Won-Sik;Jo, Seok-Su
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.20 no.4
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    • pp.2752-2759
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    • 1996
  • This paper presents crack growth analysis approach on the basis of neural networks, a branch of cognitive science to high temperature low cycle fatigue that shows strong nonlinearity in material behavior. As the number of data patterns on crack growth increase, pattern classification occurs well and two point representation scheme with gradient of crack growth curve simulates crack growth rate better than one point representation scheme. Optimal number of learning data exists and excessive number of learning data increases estimated mean error with remarkable learning time J-da/dt relation predicted by neural networks shows that test condition with unlearned data is simulated well within estimated mean error(5%).

Effects of temperature on the growth and ingestion rates of the newly described mixotrophic dinoflagellate Yihiella yeosuensis and its two optimal prey species

  • Kang, Hee Chang;Jeong, Hae Jin;Lim, An Suk;Ok, Jin Hee;You, Ji Hyun;Park, Sang Ah;Lee, Sung Yeon;Eom, Se Hee
    • ALGAE
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    • v.35 no.3
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    • pp.263-275
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    • 2020
  • Water temperature is known to affect the growth and feeding of marine dinoflagellates. Each dinoflagellate species grows well at a certain optimal temperature but dies at very cold and hot temperatures. Thus, changes in water temperatures driven by global warming and extremely high or low temperatures can affect the distribution of dinoflagellates. Yihiella yeosuensis is a mixotrophic dinoflagellate that can feed on only the cryptophyte Teleaulax amphioxeia and the chlorophyte Pyramimonas sp. Furthermore, it grows fast mixotrophically but rarely grows photosynthetically. We explored the direct and indirect effects of water temperature on the growth and ingestion rates of Y. yeosuensis feeding on T. amphioxeia and the growth rates of T. amphioxeia and Pyramimonas sp. under 7 different water temperatures (5-35℃). Both the autotrophic and mixotrophic growth rates of Y. yeosuensis on T. amphioxeia were significantly affected by temperature. Under the mixotrophic and autotrophic conditions, Y. yeosuensis survived at 10-25℃, but died at 5℃ and ≥30℃. The maximum mixotrophic growth rate of Y. yeosuensis on T. amphioxeia (1.16 d-1) was achieved at 25℃, whereas the maximum autotrophic growth rate (0.16 d-1) was achieved at 15℃. The maximum ingestion rate of Y. yeosuensis on T. amphioxeia (0.24 ng C predator-1 d-1) was achieved at 25℃. The cells of T. amphioxeia survived at 10-25℃, but died at 5 and ≥30℃. The cells of Pyramimonas sp. survived at 5-25℃, but died at 30℃. The maximum growth rate of T. amphioxeia (0.72 d-1) and Pyramimonas sp. (0.75 d-1) was achieved at 25℃. The abundance of Y. yeosuensis is expected to be high at 25℃, at which its two prey species have their highest growth rates, whereas Y. yeosuensis is expected to be rare or absent at 5℃ or ≥30℃ at which its two prey species do not survive or grow. Therefore, temperature can directly or indirectly affect the population dynamics and distribution of Y. yeosuensis.

Optimization of Culture Conditions for Production of Helicobacter pylori Adhesin Protein Genetically Linked to Cholera Toxin A2B in Escherichia coli JM101

  • Kim, Byung-Oh;Pyo, Suh-Kneung
    • Biomolecules & Therapeutics
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    • v.9 no.3
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    • pp.162-166
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    • 2001
  • Helicobacter pylori is a major cause of gastric-associated diseases. In our previous study, the Adhesin/CTXA2B was expressed as insoluble recombinant chimeric protein derived from the H. pylori adhesin genetically coupled to CTXA2B subunit in Escherichia coli. Since it is very important to optimize IPTG concentration, culture temperature and composition of medium to maximize cell growth and productivity, these conditional growth factors were determined for increasing the productivity of the expressed Adhesin/CTXA2B chimeric protein in Escherichia coli JM101 carrying pTEDhpa/ctxa2b. Our data demonstrate that optimal medium for increased production of chimeric protein was a YCP/Glu medium composed of 2% yeast extract, 1% casamino acid, phosphate solution [0.3% $KH_2P0_4$, 0.4% $Na_2HP0_4$, 0.25% ($NH_4)_2HPO_4$], and 0.5% glucose. In addition, optimal concentration of IPTG was 1 mM and culture temperature, $37^{\circ}C$.

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Cultural characterization of probiotic Lactobacillus sakei BK19

  • Yang , Byung Gyoo;Song , Choon Bok;Yeo , In Kyu;Lee , Kyoung Jun;Park , Geun Tae;Lee, Sang Hyeon;Son, Hong Joo;Heo, Moon Soo
    • Journal of fish pathology
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    • v.16 no.2
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    • pp.119-123
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    • 2003
  • We have selected an valuable pmbiotic strain; Lactobacillus sakei BK19 which has wide antagonic spectrum against fish pathogens . Present study investigated cultural characterization of L. sakei BK19 including pH tolerance , susceptibility of antibacterial agents and growth pattern with different environment such as nutritions, temperature and salinity. L. sakei BK19 showed Significantly higher resistance at low pH(around pH 4) environment and relative high antibiotic tolerance . In the study of optimal culture condition, maltose and saccharose provided the optimal nutritional culture condition while lactose and mannitol were unable to supply its carbon source for the fermentation of L. sakei BK19. Moreover. L. sakei BK19 showed good growth at the temperature range of 15 to $45^{o}C$ und the NaCl concentration of 0 to 7%. Hence, this particular probiotic strain may be benificial both in seawater and fresh weter conditions.

Ecophysiology of the kleptoplastidic dinoflagellate Shimiella gracilenta: II. Effects of temperature and global warming

  • Ok, Jin Hee;Jeong, Hae Jin;Kang, Hee Chang;Park, Sang Ah;Eom, Se Hee;You, Ji Hyun;Lee, Sung Yeon
    • ALGAE
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    • v.37 no.1
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    • pp.49-62
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    • 2022
  • Water temperature affects plankton survival and growth. The dinoflagellate Shimiella gracilenta survives using the plastids of ingested prey, indicating kleptoplastidy. However, studies on the effects of water temperature on kleptoplastidic dinoflagellates are lacking. We explored the growth and ingestion rates of S. gracilenta as a function of water temperature. Furthermore, using data on its spatiotemporal distribution in Korean coastal waters during 2015-2018, we predicted its distribution under elevated temperature conditions of +2, +4, and +6℃. Growth rates of S. gracilenta with and without Teleaulax amphioxeia prey as well as ingestion rates were significantly affected by water temperature. Growth rates of S. gracilenta with and without prey were positive or zero at 5-25℃ but were negative at ≥30℃. The maximum growth rate of S. gracilenta with T. amphioxeia was 0.85 d-1, achieved at 25℃, and 0.21 d-1 at 20℃ without prey. The ingestion rate of S. gracilenta on T. amphioxeia at 25℃ (0.05 ng C predator-1 d-1) was greater than that at 20℃ (0.04 ng C predator-1 d-1). Thus, feeding may shift the optimal temperature for the maximum growth rate of S. gracilenta from 20 to 25℃. In spring and winter, the distributions of S. gracilenta under elevated temperature conditions were predicted not to differ from those during 2015-2018. However, S. gracilenta was predicted not to survive at some additional stations under elevated temperature conditions of +2, +4, and +6℃ in summer or under elevated temperature conditions of +6℃ in autumn. Therefore, global warming may affect the distribution of S. gracilenta.

Optimization of the Sulfur-oxidzing Bacteria, Thiobacillus novellus SRM (황 산화 세균인 Thiobacillus novellus SRM 성장 최적화)

  • 권규혁;차월석;고한철;이광연;박돈희;차진명
    • KSBB Journal
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    • v.18 no.6
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    • pp.443-447
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    • 2003
  • The microorganism was isolated from the night soil treatment plant for the removal of sulfur compounds. The growth conditions of the sulfur-oxidizing bacteria were investigated and the isolate characterized as Thiobacillus noveilus SRM. The optimal pH of Thiobacillus novellus SRM on cell growth was pH 7.0 and the optimal temperature was 30$^{\circ}C$ and the optimal air flow rate was 1 vvm, respectively. As a results of cell growth from the Monod plot, the specific growth rate was 0.032 hr$\^$-l/, $V_{max}$ was 1.43 hr$\^$-l/ and $K_{m}$ was 0.32, respectively. The thiosulfate oxidation by Thiobacillus novellus SRM was made of sulfate ion. The sulfate ion reduced pH and decreased cell growth.

Effect of Temperature, pH, Carbon and Nitrogen Nutritions on Mycelial Growth of Pleurotus sajor-caju (Fr.) Sing. and Pleurotus ostreatus (Fr.) $Qu{\acute{e}}l$. (여름느타리버섯과 느타리버섯의 균계(菌系) 생장(生長)에 영향(影響)을 미치는 몇가지 요인(要因)에 관한 시험(試驗))

  • Go, Seung-Joo;You, Chang-Hyun;Park, Yong-Hwan
    • The Korean Journal of Mycology
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    • v.12 no.1
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    • pp.15-19
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    • 1984
  • Some factors affecting mycelial growth of Pleurotus sajor-caju and P. ostreatus. were investigated. The optimal temperature and pH of the medium for mycelial growth of both the species were $25^{\circ}C$ to $30^{\circ}C$, and 5 to 6, respectively. The effect of carbon and nitrogen sources on mycelial growth of the species was studied using modified Waksman's special medium as the basal medium. Generally, the disaccharides and polysaccharides showed good effect for mycelial growth of P. sajor-caju, and the polysaccharides were superior to the other classes of carbon sources for mycelial growth of P. ostreatus. Generally the organic nitrogen compounds showed good mycelial growth of both the species. The mycelial growth of the species were favored by the ammonium nitrogen more than by the nitrate nitrogen source. Asparagine was superior to the other kinds of amino acid. Six percentage of glucose and 0.6% ammonium tartarate was optimum for mycelial growth of P. sajor-caju whereas the optimum concentration for the P. ostreatut was 4% glucose and 0.4% ammonium tatarate.

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