• Title/Summary/Keyword: Open

Search Result 25,557, Processing Time 0.048 seconds

Expression of Organogenesis-related Genes and Analysis of Genetic Stability by ISSR Markers of Regenerants Derived from the Process of in vitro Organogenesis in Japanese Blood Grass (Imperata cylindrica 'Rubra') (기내배양 홍띠 단계별 재분화체의 기관분화 관련 유전자 발현과 ISSR에 기반한 유전적 안정성 분석)

  • Ye-Jin Lee;In-Jin Kang;Chang-Hyu Bae
    • Korean Journal of Plant Resources
    • /
    • v.36 no.5
    • /
    • pp.496-507
    • /
    • 2023
  • The in vitro organogenesis is one of important issues in plant embryology, and somaclonal variations are existing in calli and/or regenerants induced from a process of the organogenesis with in vitro circumstances. In this study, expressions of organogenesis-related genes were evaluated and genetic stability of regenerants derived from the process of in vitro organogenesis were measured using ISSR markers in Imperata cylindrica 'Rubra', Poaceae. The expressions of organogenesis-related genes were detected all of regenerants at the process of the organogenesis. All ISSR markers produced with an average of 71 bands per in vitro-cultured regenerants, and the scorable bands were varied from two to eight with an average of 5.14 bands per a primer. The polymorphism rates of the in vitro regenerants were higher than that of mother plants (1.4%), showing 4.1% (pot-cultured regenerants), 4.3% (field-cultured regenerants), 4.2% (in vitro-cultured regenerants), 5.6% (calli with green shoots) and 1.4% (calli), respectively. The genetic similarity matrix (GSM) among all accessions ranged from 0.747 to 1.0 with a mean of 0.868. GSM of the regenerants showed differences (from 0.972 to 1.00) compared with that of mother plants (0.991). According to the clustering analysis, two independent groups were divided into; the one is mother plants and regenerants cultured at room and open field, the other is regenerants cultured in vitro. The results give a new insight for understanding the dynamics of organogenesis in monocot plant.

Development of Genetic Selection Marker via Examination of Genome in Bacillus velezensis K10 (Bacillus velezensis K10 유전체 분석을 통한 균주 선발 마커 개발)

  • Sam Woong Kim;Young Jin Kim;Tae Wook Lee;Won-Jae Chi;Woo Young Bang;Tae Wan Kim;Kyu Ho Bang;Sang Wan Gal
    • Journal of Life Science
    • /
    • v.33 no.11
    • /
    • pp.897-904
    • /
    • 2023
  • This study was done to develope genetic markers with the unique characteristics of genes according to the genomic information of Bacillus velezensis K10. B. velezensis K10 maintained a total of 4,159,835 bps, which was found to encode 5,136 open reading frames (orfs). B. velezensis K10 was found to have much more gene migration due to external factors overall compared to standard strain B. velezensis JS25R. In order to discover genetic selection markers, orfs on the genome to be easily induced to gene mutation were surveyed such as recombinase, integrase, transposase, and phage-related genes. As a result of the investigation, 9 candidate markers were isolated with high possibility as genetic selection markers. Although a part in the various origin's areas showed specificities in comparison with homology, the selected markers were all existed in phage-related areas because they were relatively lower homologies in phage-related genes. PCR analysis was done on B. licheniformis K12, B. velezensis K10, B. subtilis, and B. cereus to establish them as inter-species candidate selection markers. As a result, it was confirmed that B. velezensis K10-specific PCR products were formed in a total of 6 primer sets such as BV3 and BV5 to 9. On the other hand, analysis at the subspecies level observed the formation of B. velezensis K10-specific PCR products in 4 primer sets such as BV3, 5, 8, and 9. Among them, since BV5 and BV8 were detected by very specific results, we suggest that BV5 and 8 can be used as B. velezensis K10 gene selection markers at the species and sub-species level.

Forming and Changing the Concept of 'Cultural Property' before the Enactment of the Cultural Heritage Protection Act (문화재보호법 제정 이전 '문화재' 개념의 형성과 변화)

  • OH Chunyoung
    • Korean Journal of Heritage: History & Science
    • /
    • v.56 no.4
    • /
    • pp.288-318
    • /
    • 2023
  • This work began with the aim of examining the history of the concept "cultural property" that is expected to disappear, and the main subject of research was the history that preceded the spread of this notion throughout society. The phrase "cultural property" first appeared in the 1920s, and was used in various fields such as literature, history, music, and philosophy in the context of cultural resources. Until immediately following liberation from the Japanese colonial era, the meaning of cultural assets was widely applied in the range of "cultural resources," and during this period, it was often used to help supplant the reality and history of Japanese occupation. Immediately after the Korean War, it was also employed for the purpose of 'restoration of cultural resources through war'. Recognition of cultural property directly influenced by Japan's Cultural Heritage Protection Act has occurred since 1950s. In the early 1960s, the enactment of various laws related to cultural properties and the establishment of the Cultural Heritage Administration caused the meaning of cultural property to be limited to 'cultural heritage'. In this way, the definition of state-led cultural property has continued to apply to this day. It has not been clearly confirmed whether the concept of cultural properties was imported from Japan through means such as the Cultural Heritage Protection Act. Cases in which several Japanese students endorsed the concept of cultural property within Korea serve to increase the likelihood that the concept was indeed imported from Japan. However, "coined language using multiple Chinese characters," "the phenomenon of cultural complex words in the 1920s,", and "cases of non-Japanese international students using the concept of cultural property" also open up the possibility of their own occurrence. Apart from the general importance of the concept of cultural property, intellectuals at the time used this concept to promote internal development and the overcoming of colonial Joseon. In this research, it was confirmed that the conceptual word cultural property was older and had a wider history than the general perception had indicated previously. The history of the conceptual term "cultural property" may appear to be more than 60 years old based on the enactment of the Cultural Heritage Protection Act, but in fact it is nearly 100 years old when traced back to on 1925, as established here. In general, the creation and disappearance of terms may proceed naturally with social change, but such terms may alternatively be created or erased through national policy. Identifying the origins of a phrase that is about to disappear represents a significant task for purposes of establishing its historical meaning.

Classification of Carbon-Based Global Marine Eco-Provinces Using Remote Sensing Data and K-Means Clustering (K-Means Clustering 기법과 원격탐사 자료를 활용한 탄소기반 글로벌 해양 생태구역 분류)

  • Young Jun Kim;Dukwon Bae;Jungho Im ;Sihun Jung;Minki Choo;Daehyeon Han
    • Korean Journal of Remote Sensing
    • /
    • v.39 no.5_3
    • /
    • pp.1043-1060
    • /
    • 2023
  • An acceleration of climate change in recent years has led to increased attention towards 'blue carbon' which refers to the carbon captured by the ocean. However, our comprehension of marine ecosystems is still incomplete. This study classified and analyzed global marine eco-provinces using k-means clustering considering carbon cycling. We utilized five input variables during the past 20 years (2001-2020): Carbon-based Productivity Model (CbPM) Net Primary Production (NPP), particulate inorganic and organic carbon (PIC and POC), sea surface salinity (SSS), and sea surface temperature (SST). A total of nine eco-provinces were classified through an optimization process, and the spatial distribution and environmental characteristics of each province were analyzed. Among them, five provinces showed characteristics of open oceans, while four provinces reflected characteristics of coastal and high-latitude regions. Furthermore, a qualitative comparison was conducted with previous studies regarding marine ecological zones to provide a detailed analysis of the features of nine eco-provinces considering carbon cycling. Finally, we examined the changes in nine eco-provinces for four periods in the past (2001-2005, 2006-2010, 2011-2015, and 2016-2020). Rapid changes in coastal ecosystems were observed, and especially, significant decreases in the eco-provinces having higher productivity by large freshwater inflow were identified. Our findings can serve as valuable reference material for marine ecosystem classification and coastal management, with consideration of carbon cycling and ongoing climate changes. The findings can also be employed in the development of guidelines for the systematic management of vulnerable coastal regions to climate change.

The basis of trust in relationships: Indigenous psychological analysis of adolescents and their parents (청소년과 부모의 인간관계를 통해 본 신뢰의식: 토착심리학적 접근)

  • Uichol Kim;Young-Shin Park
    • Korean Journal of Culture and Social Issue
    • /
    • v.10 no.2
    • /
    • pp.103-137
    • /
    • 2004
  • This study examines the basis of trust in relationships by adolescents and their parents using the indigenous psychological approach. Using the indigenous methodology, adolescents were asked the reasons why they trusted their mother, father, friends, and teachers. Parents were asked why they trusted their children, spouse and their children's teachers. A total of 1,737 participants completed an open-ended survey: 579 adolescents (274 middle school and 305 high school students) and their parents (579 fathers) and (579 mothers). The results indicate that adolescents trust their parents because of their sacrifice, followed by consanguinity (i.e., blood relationship), respect, their trust in me, dependability, and their advice and counseling. The reasons why adolescents trust their teachers is because of the academic guidance they provide, unconditional trust of teachers, their concern and care, respect for teachers, advice and counseling they provide, they are like parents, and because of their sacrifice for the students. The reasons for trusting their friends are as follows: Dependability, closeness, unconditional trust of friends, their understanding of me, and their emotional support. The reasons why parents trust their children are: Children's sincerity, honesty, consanguinity, parents' expectation and communication with the children, children's obedience, and since they are diligent in their schoolwork. The reasons for trusting one's spouse are reported to be sincerity, their sacrifice for the family, honesty, unconditional trust of a spouse, and because of mutual support. The reasons why parents trust their children's teachers are reported as follows: Unconditional trust of teachers, their sacrifice for the students, and their sincerity. There were no significant differences across the type of school and academic grades in terms of trust of parents. However, middle school students are more likely to trust their teachers, and high school students are more likely trust their friends. The male students rather than female students and those students with higher academic grades are more likely to trust their parents, friends, and teachers. For parents, there were no significant differences across age, sex, and educational status concerning the trust of their children, spouse, and children's teachers. There was a positive correlations between parents' trust of their spouse and children and their children's trust of their parents. There was also a positive correlations of mothers' trust of children's teachers and the children's trust of their teachers.

  • PDF

The social representation and trust of Korean society and people: Indigenous psychological analysis of the perception of Korean adolescents and adults (한국 사회와 사람에 대한 사회적 표상과 신뢰: 청소년과 성인의 지각을 통해 본 토착심리 분석)

  • Uichol Kim ;Young-Shin Park
    • Korean Journal of Culture and Social Issue
    • /
    • v.10 no.3
    • /
    • pp.103-129
    • /
    • 2004
  • This article examines the Korean adolescents and adults' social representation and trust of Korean society and people using indigenous psychological analysis. Respondents were asked to write in an open-ended questionnaire their perception of the following five aspects: Korean politics, economy, society, culture and people. They were then asked to report why they trust or distrust Korean society. A total of 1,064 respondents (218 middle school students, 200 university students, 218 fathers of the middle school students, 218 mothers of the middle school students, and 210 teachers) completed a questionnaire developed by the present researchers. The data were collected during April to June, 2003. The results indicate that 94.5% of Koreans view the existing political system and politicians as being corrupt, inept, factional, and lacking in integrity. A vast majority (84.9%) recognize the existence of systemic problems in the Korean economy. A total of 78.2% see problems in Korean society being dominated by selfishness, factionalism, conservatism, and social uncertainty. For Korean culture, a majority of respondents report being proud of its cultural tradition, accomplishment, and creativity. At the same time, 45.7% report loss of cultural identity and pride due to external influences. More than half of the respondents report negative aspects of Korean people (i.e., selfish, lack of morality, rushed, and overly focused on their social image), while nearly half of the respondents report positive aspects of Korean people as being compassionate, cooperative, good-natured and hard-working. As for reason for trusting Korean society, around a third report "because it is our country," followed by its future potential, and the good-nature and willingness of Korean people to work hard. The reasons for distrusting Korean society is the dishonesty politicians, corruption, institutional ineptness, and economic uncertainty. These results indicate a low level of collective efficacy in influencing and affecting change in Korean society.

  • PDF

A Study on the Support Method for Activate Youth Start-ups in University for the Creation of a Start-up Ecosystem: Focused on the Case of Seoul City (지역 청년창업생태계 조성을 위한 대학의 지원방안 탐색: 서울시 사례를 중심으로)

  • Kim, In Sook;Yang, Ji Hee
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
    • /
    • v.17 no.4
    • /
    • pp.57-71
    • /
    • 2022
  • The purpose of this study was to analyze the perception and demand of local youth and to find ways to support universities in order to create an youth start-up ecosystem. To this end, 509 young people living in Seoul were analyzed to recognize and demand young people in the region for youth start-ups, and to support universities. The findings are as follows. First, as a result of analyzing young people's perception of youth start-ups in the region, the "Youth Start-up Program" was analyzed the highest in terms of the demand for regional programs by university. In addition, there was a high perception that the image of youth startups in the region was "challenging" and "good for changing times." Second, after analyzing the demand for support for youth start-ups in the region, it appeared in the order of mentoring, start-up education, and creation of start-up spaces. And it showed different needs for different ages. Third, the results were derived from analysis of the demand for university support for the creation of a regional youth start-up ecosystem, the criteria for selecting local youth start-up support organizations, and the period of participation in local youth start-up support. Based on the results of the above research, the implications and suggestions of university support for the creation of a community of youth start-up ecosystem are as follows. First of all, it is necessary to develop and operate sustainable symbiosis mentoring programs focusing on university's infrastructure and regional symbiosis. Second, it is necessary to develop and utilize step-by-step systematic microlearning content based on the needs analysis of prospective youth start-ups. Third, it is necessary to form an open youth start-up base space for local residents in universities and link it with the start-up process inside and outside universities. The results of this study are expected to be used as basic data for establishing policies for supporting youth start-ups and establishing and operating strategies for supporting youth start-ups at universities.

A Study on the Needs Analysis of University-Regional Collaborative Startup Co-Space Composition (대학-지역 연계 협업적 창업공간(Co-Space) 구성 요구도 분석)

  • Kim, In-Sook;Yang, Ji-Hee;Lee, Sang-Seub
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
    • /
    • v.18 no.1
    • /
    • pp.159-172
    • /
    • 2023
  • The purpose of this study is to explore a collaborative start-up space(Co-Space) configuration plan in terms of university-regional linkage through demand analysis on the composition of university-regional linkage startup space. To this end, a survey was conducted for request analysis, and the collected data were analyzed through the t-test, The Lotus for Focus model. In addition, FGI was implemented for entrepreneurs, and the direction of the composition of the university-region Co-Space was derived from various aspects. The results of this study are as follows. First, as a result of the analysis of the necessity of university-community Co-Space, the necessity of opening up the start-up space recognized by local residents and the necessity of building the start-up space in the region were high. In addition, men recognized the need to build a space for start-ups in the community more highly than women did women. Second, as a result of analysis of demands for university-regional Co-Space, the difference between current importance and future necessity of university-regional Co-Space was statistically significant. Third, as a result of analysis on the composition of the startup space by cooperation between universities and regions, different demands were made for composition of the startup space considering openness and closeness, and for composition of the startup space size. The implications of the study are as follows. First, Co-Spaces need to be constructed in conjunction with universities in accordance with the demands of start-up companies in the region by stage of development. Second, it is necessary to organize a customized Co-Space that takes into account the size and operation of the start-up space. Third, it is necessary to establish an experience-based open space for local residents in the remaining space of the university. Fourth, it is necessary to establish a Co-Space that enables an organic network between local communities, start-up investment companies, start-up support institutions, and start-up companies. This study is significant in that it proposed the regional startup ecosystem and the cooperative start-up space structure for strengthening start-up sustainability through cooperation between universities and local communities. The results of this study are expected to be used as useful basic data for Co-Space construction to build a regional start-up ecosystem in a trend emphasizing the importance of start-up space, which is a major factor affecting start-up companies.

  • PDF

A Study on the Social Venture Startup Phenomenon Using the Grounded Theory Approach (근거이론 접근법을 이용한 소셜벤처 창업 현상에 관한 고찰)

  • Seol, Byung Moon;Kim, Young Lag
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
    • /
    • v.18 no.1
    • /
    • pp.67-83
    • /
    • 2023
  • The social venture start-up phenomenon is found from the perspectives of social enterprise and for-profit enterprise. This study aims to fundamentally explore the start-up phenomenon of social ventures from these two perspectives. Considering the lack of prior research that researched both social and commercial perspectives at the same time, this paper analyzed using grounded theory approach of Strauss & Corbin(1998), an inductive research method that analyzes based on prior research and interview data. In order to collect data for this study, eight corporate representatives currently operating social ventures were interviewed and data and phenomena were analyzed. This progressed to a theoretical saturation where no additional information was derived. The analysis results of this study using the grounded theory approach are as follows. As a result of open coding and axial coding, 147 concepts and 70 subcategories were derived, and 18 categories were derived through the final abstraction process. In the selective coding, 'expansion of social venture entry in the social domain' and 'expansion of social function of for-profit companies' were selected as key categories, and a story line was formed around this. In this study, we saw that it is necessary to conduct academic research and analysis on the competitive factors required for companies that pursue the values of two conflicting relationships, such as social ventures, to survive with competitiveness. In practice, concepts such as collaboration with for-profit companies, value combination, entrepreneurship competency and performance improvement, social value execution competency reinforcement, communication strategy, for-profit enterprise value investment, and entrepreneur management competency were derived. This study explains the social venture phenomenon for social enterprises, commercial enterprises, and entrepreneurs who want to enter the social venture field. It is expected to provide the implications necessary for successful social venture startups.

  • PDF

The Effect of Satisfaction Level with the University Entrepreneurship Education, Recognition of Support System, and Mentoring on the Entrepreneurial Intention: The Moderating Effect of Entrepreneurial Self-Efficacy (대학생의 창업교육 만족도와 창업지원제도인식, 창업멘토링이 창업의지에 미치는 영향: 창업효능감을 조절효과로)

  • Yu, Young Cheul;Lee, Won Il
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
    • /
    • v.18 no.2
    • /
    • pp.187-200
    • /
    • 2023
  • The purpose of this study is to examine how the start-up education, start-up support system, and start-up mentoring directly and indirectly conducted in universities affects entrepreneurial Intention, and to present programs and directions that can increase the possibility of start-up for college students. This study analyzed the effects of college students' satisfaction with university entrepreneurship education, awareness of the entrepreneurship support system, and entrepreneurship mentoring on entrepreneurial will, and then analyzed how entrepreneurship efficacy affects entrepreneurial will as a moderating effect. The recognition of the start-up support system was divided into the government start-up support policy and the university start-up support project, and start-up mentoring was divided into mentoring function (problem-solving function, networking function, communication function, motivation function) and mentor trust (cognitive trust, emotional trust). Regression analysis was performed on satisfaction with college entrepreneurship education, perception of entrepreneurship support system, entrepreneurship mentoring as independent variables, and willingness to entrepreneurship as dependent variables. In addition, entrepreneurship efficacy was designated as a moderating variable and analyzed. As a result of the study, first, it was found that satisfaction with start-up education had a positive (+) effect on college students' willingness to start a business. Second, among the start-up support systems, university start-up support projects were found to have a positive (+) effect on college students' willingness to start a business. Third, as a result of verifying whether entrepreneurial self-efficacy has a moderating effect on the relationship between college start-up support projects and college students' willingness to start a business while recognizing the start-up support system, it was found to have a moderating effect. The following implications can be derived based on the analysis results of this study. First, efforts to improve start-up education will be needed to increase the satisfaction of start-up education. Second, Through the operation of the start-up counseling center in the university, it will be possible to recognize the education system supported by the university, the university start-up support project that can carry out the indirect experience of start-up, and the government start-up support policy that supports funds. Third, It will be necessary to open a program that can provide start-up mentoring through connection with mentors in the start-up field, such as professors, employees, and senior start-ups in universities. Fourth, It is necessary to develop thorough education on the preparation process of start-ups, start-up special lectures where senior start-ups can indirectly experience the failure of start-ups, and programs for customized start-up education according to college students' major and individual tendencies.

  • PDF