• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.74.1-74
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• 2003

Differential display (DD) of mRNA is a technique in which mRNA species expressed by a cell population are reverse transcribed and then amplified by many separate polymerase chain reactions (PCR). Using DD-RT-PCR we obtained many genes that expressed differentially in healthy and PVX-infected Nicotiana benthamima, using total RNAs extracted from healthy and PVX-infected N. benthamiana plants. Three hundred and twenty-five DNA fragments isolated from DD-RT-PCR were cloned and sequenced for further characterization. Several host genes including SKPI-like protein, heat shock transcription factor and Avr9/Cf-9 rapidly elicited protein were selected to obtain full-length open reading frame and to characterize their potential involvement in virus disease development and/or host's defense against virus infection employing PVX-based expression vector. Transcrips from wild-type and clones containing each selected gene were inoculated onto N. benthamiana Levels of virus replication were confirmedby RT-PCR and RNA blot analysis, Expression profiles and potential role(s) of selected genes upon PVX infection will be discussed.

• Journal of Advanced Marine Engineering and Technology
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• 제24권1호
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• pp.49-58
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• 2000

Thermal spraying is one of the most common surface coating techniques to be used for many applications and flame spraying covers a wide range of different materials which can be coated onto various substrates. The purpose of this study is to investigate the effects of mixed ratio in composite coatings on the mechanical and anti-corrosion properties. The five different types of composite coatings were made with $Al_2O_3$ ceramic and Ni-alloy powder on the mild steel substrate by flame spraying method. The mechanical properties such as microhardness, adhesive strength and erosion resistance and corrosion resistance were tested for the sprayed coating specimens. The results obtained are summarized as follows; 1. The composite coating layers greatly improve the microstructure, erosion resistance and adhesive strength by increasing the content of Ni-Al alloy. 2. Microhardness of the compsite coating layer is decreased by increasing the content of Ni-Al alloy. 3. The anti-corrosion properties is considerably improved by increasing the compsite rate of Ni-Al alloy.

• 한국정밀공학회지
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• 제26권8호
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• pp.47-54
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• 2009

In this study, a simple and effective method was proposed to minimize electrolytic corrosion on the workpiece during ED-drilling using water as a working fluid. The adhesion of a cover plate onto the surface of the workpiece was greatly effective for suppressing electrolytic corrosion during ED-drilling. The experiment revealed that the adhesion of the cover plate prevented corrosion without causing significant changes in machining characteristics. Using the machining method proposed in this paper, electrolytic-corrosion-free holes can be machined without change in the machinery system. By using corrosion-free hole as a start hole for wire EDM, a lead frame die with high quality was fabricated successfully.

• 한국HCI학회:학술대회논문집
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• 한국HCI학회 2009년도 학술대회
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• pp.253-256
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• 2009

본 논문은 기존의 단일 분야를 대상으로 서비스되고 있는 버티컬 검색의 한계를 지적하고 사용자의 검색 욕구를 보다 충실히 만족시키기 위해, 여러 분야 (개체 유형)들을 포함하는 실체 (개체)들이나 단일 분야 내의 실체들을 포함하는 질의어를 처리할 수 있는 다중 개체 중심적 통합 방식의 버티컬 검색을 제시한다. 이를 위해, 질의어를 분석하여 개체 유형 간 결합이 필요한 지를 판단한 후 동적으로 상황에 맞는 서비스 컴포넌트들을 결합하는 기술과 개체 유형 별 필드들을 구축하고 필드 별 검색을 수행하는 기술을 도입하였다. 버티컬 검색 서비스 분야로서 학술 연구 정보를 대상으로 하여 약 453,000 편의 해외 학술 저널 논문을 메타데이터 기반으로 등록하였으며, 개체 유형으로는 연구 주제와 연구자를 다루고 있다.

• 한국실내디자인학회논문집
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• 제16권1호
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• pp.48-55
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• 2007

Proceedings in a new field of sciences and technologies like genetic engineering challenge to conventional concepts. It demonstrates a certain disappearance in conventional concepts that dominants our perceptions, such as organic, mechanical, and dichotomic concept of informative materials, and that is to be extended as a potential possibility. Then, what is a new space where our bodies that feel and cherish in their mind should live in these days as well as it makes senses by itself and should affect the principle that makes worlds? In addition, this study proposes a frame in the analysis in order to understand a section of modern designs with the question of 'what is the change in the relationship between relative structures in spaces and objects from a design point of view according to new structural concepts of body?' The major point of this analysis can be realized based on the assumption of the extension of the characteristics in the change of structural relationship in the aspect of post structuralism that includes non-structuralized relationship represented in modern philosophy, arts, and architectural works. Then, it can be accomplished as positive ideas in the foundation of space designs in future including the understand of 'new structural relationship' that can't be expressed as rationality and causal sequence by considering how the experiment conducted using several topics on body can be projected onto spaces through the process applied in the experiment.

• Journal of Electrical Engineering and Technology
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• 제12권4호
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• pp.1675-1682
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• 2017

This paper deals with three dimensional orientation estimation algorithm for an attitude and heading reference system (AHRS) based on nine-axis inertial/magnetic sensor signals. In terms of the orientation estimation based on the use of a Kalman filter (KF), the quaternion is arguably the most popular orientation representation. However, one critical drawback in the quaternion representation is that undesirable magnetic disturbances affect not only yaw estimation but also roll and pitch estimations. In this paper, a sequential direction cosine matrix-based orientation KF for AHRS has been presented. The proposed algorithm uses two linear KFs, consisting of an attitude KF followed by a heading KF. In the latter, the direction of the local magnetic field vector is projected onto the heading axis of the inertial frame by considering the dip angle, which can be determined after the attitude KF. Owing to the sequential KF structure, the effects of even extreme magnetic disturbances are limited to the roll and pitch estimations, without any additional decoupling process. This overcomes an inherent issue in quaternion-based estimation algorithms. Validation test results show that the proposed method outperforms other comparison methods in terms of the yaw estimation accuracy during perturbations and in terms of the recovery speed.

• 대한기계학회논문집B
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• 제26권12호
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• pp.1715-1721
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• 2002

Numerical analysis was conducted to characterize the gas flow field and particle deposition on a horizontal freestanding semiconductor wafer under the laminar flow field at vacuum environment. In order to calculate the properties of gas, the gas was assumed to obey the ideal gas law. The particle transport mechanisms considered were convection, Brownian diffusion and gravitational settling. The averaged particle deposition velocities and their radial distributions fnr the upper surface of the wafer were calculated from the particle concentration equation in an Eulerian frame of reference for system pressures of 1 mbar~1 atm and particle sizes of 2nm~10$^4$ nm(10 ${\mu}{\textrm}{m}$). It was observed that as the system pressure decreases, the boundary layer of gas flow becomes thicker and the deposition velocities are increased over the whole range of particle size. One thing to be noted here is that the deposition velocities are increased in the diffusion dominant particle size range with decreasing system pressure, whereas the thickness of the boundary layer is larger. This contradiction is attributed to the increase of particle mechanical mobility and the consequent increase of Brownian diffusion with decreasing the system pressure. The present numerical results showed good agreement with the results of the approximate model and the available experimental data.

• Journal of Microbiology and Biotechnology
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• 제2권1호
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• pp.56-65
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• 1992

The complete nucleotide sequence of the Bacillus circulans F-2 RSDA gene, coding for raw starch digesting a-amylase (RSDA), has been determined. The RSDA structure gene consists of an open reading frame of 2508 bp. Six bp upstream of the translational start codon of the RSDA is a typical gram-positive Shine-Dalgarno sequence and the RSDA encodes a preprotein of 836 amino acids with an Mr of 96, 727. The gene was expressed from its own regulatory region in E. coli and two putative consensus promoter sequences were identified upstream of a ribosome binding site and an ATG start codon. Confirmation of the nucleotide sequence was obtained and the signal peptide cleavage site was identified by comparing the predicted amino acid sequence with that derived by N-terminal analysis of the purified RSDA. The deduced N-terminal region of the RSDA conforms to the general pattern for the signal peptides of secreted prokaryotic proteins. The complete amino acid sequence was deduced and homology with other enzymes was compared. The results suggested that the Thr-Ser-rich hinge region and the non-catalytic domain are necessary for efficient adsorption onto raw substrates, and the catalytic domain (60 kDa) is necessary for the hydrolysis of substrates, as suggested in previous studies (8, 9).

• Earthquakes and Structures
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• 제8권4호
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• pp.915-934
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• 2015

Within the context of Structural Health Monitoring (SHM), it is often the case that structural systems are described by uncertainty, both with respect to their parameters and the characteristics of the input loads. For the purposes of system identification, efficient modeling procedures are of the essence for a fast and reliable computation of structural response while taking these uncertainties into account. In this work, a reduced order metamodeling framework is introduced for the challenging case of nonlinear structural systems subjected to earthquake excitation. The introduced metamodeling method is based on Nonlinear AutoRegressive models with eXogenous input (NARX), able to describe nonlinear dynamics, which are moreover characterized by random parameters utilized for the description of the uncertainty propagation. These random parameters, which include characteristics of the input excitation, are expanded onto a suitably defined finite-dimensional Polynomial Chaos (PC) basis and thus the resulting representation is fully described through a small number of deterministic coefficients of projection. The effectiveness of the proposed PC-NARX method is illustrated through its implementation on the metamodeling of a five-storey shear frame model paradigm for response in the region of plasticity, i.e., outside the commonly addressed linear elastic region. The added contribution of the introduced scheme is the ability of the proposed methodology to incorporate uncertainty into the simulation. The results demonstrate the efficiency of the proposed methodology for accurate prediction and simulation of the numerical model dynamics with a vast reduction of the required computational toll.

• 한국멀티미디어학회논문지
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• 제17권10호
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• pp.1189-1197
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• 2014

Recently, general purpose graphic processing units(GPUs) are being widely used in mobile embedded systems such as smart phone and tablet PCs. Because of architectural limitations of mobile GPGPUs, only a single program is allowed to occupy a GPU at a time in a non-preemptive way. As a result, it is difficult to meet performance requirements of applications such as frame rate or response time if applications running on a GPU are not scheduled properly. To tackle this difficulty, we propose to specify applications using synchronous data flow model of computation such that applications are formed with edges and nodes. Then nodes of applications are scheduled onto a GPU unlike conventional scheduling an application as a whole. This approach allows applications to share a GPU at a finer granularity, node (or task)-level, providing several benefits such as eliminating need for manually partitioning applications and better GPU utilization. Furthermore, any scheduling policy can be applied in response to the characteristics of applications.