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Establishment of the Chickpea Wilt Pathogen Fusarium oxysporum f. sp. ciceris in the Soil through Seed Transmission

  • Pande S.;Rao, J. Narayana;Sharma M.
    • The Plant Pathology Journal
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    • v.23 no.1
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    • pp.3-6
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    • 2007
  • Chickpea wilt caused by Fusarium oxysporum f. sp. ciceris(FOC) is the most destructive disease in India. It is seed-borne as well as soil-borne pathogen. The role of seed-borne FOC in introducing and establishing wilt in FOC free soils is unknown. Using seeds of FOC infected chickpea cultivar K 850, we provided an evidence of establishing wilt disease in the FOC free soils within three crop cycles or seasons. In the first cycle, typical wilt symptoms were observed in 24 pots in 41 days after sowing. These 24 pots were used for second and third cycles without changing the soil. These 24 pots were sown with seeds collected from healthy plants of a susceptible cultivar JG 62, one seed per pot and development of wilt symptom was recorded. Wilt symptoms appeared in all the pots 26 days after sowing in second cycle and in 16 days after sowing in third cycle. On selective medium, all of the wilted plants yielded FOC in all the three cycles indicating that the mortality was due to wilt. FOC propagules on selective medium were 172, 1197, and 2280 $g^{-1}$ soil at the end of the first, second, and third cycles, respectively. These studies indicated that Fusarium wilt of chickpea is seed-borne and seeds harvested from wilted plants when mixed with healthy seeds can carry the wilt fungus to new areas and can establish the disease in the soil to economic threshold levels within three seasons.

In vitro plantlet regeneration of "dwarf" Indian olive (Elaeocarpus robustus Roxb.): a fruit plant of Bangladesh

  • Rahman, Md. Mahabubur;Amin, Muhammad Nurul;Ishiguri, Futoshi;Yokota, Shinso;Sultana, Rubaiyat Sharmin;Takashima, Yuya;Iizuka, Kazuya;Yoshizawa, Nobuo
    • Plant Biotechnology Reports
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    • v.3 no.3
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    • pp.259-266
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    • 2009
  • A plantlet regeneration protocol was developed on pot-grown mature plants of Elaeocarpus robustus Roxb. cv. Dwarf from nodal and leaf explants. The best yield of adventitious shoots was achieved from the leaf-derived calli in a modified MS ($MMS_1$, half strength of major salts, full strength of minor salts, and vitamins) medium containing $4.0{\mu}M$ BA + $4.0{\mu}M$ Kn + $0.5{\mu}M$ NAA + 15% coconut water (CW). The shoot multiplication rate was amplified about twofold per culture after the addition of 15% CW to the medium. The rate of shoot multiplication reached maximum at the 5th subculture, and it maintained this rate throughout the 3 subsequent subcultures. The best rooting in vitro was investigated by subculturing the microcuttings in an $MMS_2$ (half strength of both major salts and minor salts and full strength of vitamins) medium containing $1.0{\mu}M$ IBA in the dark for one initial week at $30^{\circ}C$, followed by subculturing them in a plant-growth regulator (PGR)-free medium in the light. The plantlets raised in vitro were successfully established under ex vitro conditions.

Preparation of Fabric Softener Product by using Amine-functionalized Magnesium-phyllosilicates (아민기로 관능화된 마그네슘-층상규산염을 이용한 섬유유연제 제조)

  • Kim, Seong Yeol;Choi, Yoo-Sung
    • Applied Chemistry for Engineering
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    • v.30 no.5
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    • pp.580-585
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    • 2019
  • In this study, we synthesized amine-functionalized magnesium-phyllosilicates (AF-MgP) with an octahedral and tetrahedral structure using (3-aminopropyl)triethoxysilane. The synthesis of AF-MgP, surface functionalization of amine and 1 : 2 ratio of the octahedral and tetrahedral structure were confirmed by FT-IR and XRD analysis. In addition, it was confirmed that AF-MgP was absorbed evenly on the surface of cotton fibers and coated on the cotton fibers from HR-SEM and EDX analysis. The antimicrobial activity test of cotton fibers according to KS confirmed that cotton fibers coated with AF-MgP particles show an enhanced antimicrobial activity against cutaneous microorganisms. Our results suggest that AF-MgP is not only applied as a functional nanomaterial that gives the cotton fiber antimicrobiality, but also can be used in the field of cosmetic and biomedical materials.

Development of Antagonistic Microorganism for Biological Control of Dollar Spot of Turfgrass (잔디 동전마름병의 생물학적 방제를 위한 길항 미생물의 선발과 효력 검정)

  • Shim, Taek-Su;Jung, Woo-Cheol;Do, Ki-Seok;Shim, Gyu-Yul;Lee, Jae-Ho;Choi, Kee-Hyun
    • Asian Journal of Turfgrass Science
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    • v.20 no.2
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    • pp.191-201
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    • 2006
  • Dollar spot caused by Sclerotinia homeocarpa is one of major diseases in putting greens. Microorganisms antagonistic to S. homeocarpa, a pathogen of dollar spot, were primarily screened through in vitro tests, including dual culture method and triple layer agar diffusion method. In vivo tests were also conducted to select the best candidate for a biocontrol microorganism, using pot experiment. Bacillus subtilis EW42-1 and Trichoderma harziaum GBF-0208 were finally selected as biocontrol agents against dollar spot. Relative Performance Index(RPI) was used as a criterion of selecting potential biocontrol agents. B. subtilis EW42-1 and T. harzianum GBF-0208 showed resistance to several agrochemicals mainly used in a golf course. B. subtilis EW42-1 and T. harzianum GBF-0208 suppressed effectively the disease progress of dollar spot like synthetic fungicide tebuconazole in the nursery where dollar spot had seriously occurred. B. subtilis EW42-1 and T. harzianum GBF-0208 have a potential to be biocontrol agents for the control of dollar spot.

Alanine and serine functionalized magnetic nano-based particles for sorption of Nd(III) and Yb(III)

  • Galhoum, Ahmed A.;Mahfouz, Mohammad G.;Atia, Asem A.;Gomaa, Nabawia A.;Abdel-Rehem, Sayed T.;Vincent, Thierry;Guibal, Eric
    • Advances in environmental research
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    • v.5 no.1
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    • pp.1-18
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    • 2016
  • Magnetic nano-based sorbents have been synthesized for the recovery of two rare earth elements (REE: Nd(III) and Yb(III)). The magnetic nano-based particles are synthesized by a one-pot hydrothermal procedure involving co-precipitation under thermal conditions of Fe(III) and Fe(II) salts in the presence of chitosan. The composite magnetic/chitosan material is crosslinked with epichlorohydrin and modified by grafting alanine and serine amine-acids. These materials are tested for the binding of Nd(III) (light REE) and Yb(III) (heavy REE) through the study of pH effect, sorption isotherms, uptake kinetics, metal desorption and sorbent recycling. Sorption isotherms are well fitted by the Langmuir equation: the maximum sorption capacities range between 9 and 18 mg REE $g^{-1}$ (at pH 5). The sorption mechanism is endothermic (positive value of ${\Delta}H^{\circ}$) and contributes to increase the randomness of the system (positive value of ${\Delta}S^{\circ}$). The fast uptake kinetics can be described by the pseudo-second order rate equation: the equilibrium is reached within 4 hours of contact. The sub-micron size of sorbent particles strongly reduces the contribution of resistance to intraparticle diffusion in the control of uptake kinetics. Metal desorption using acidified thiourea solutions allows maintaining sorption efficiency for at least four successive cycles with limited loss in sorption capacity.

Castor oil based hyperbranched polyester/bitumen modified fly ash nanocomposite

  • Bhagawati, Deepshikha;Thakur, Suman;Karak, Niranjan
    • Advances in nano research
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    • v.4 no.1
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    • pp.15-29
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    • 2016
  • A low cost environmentally benign surface coating binder is highly desirable in the field of material science. In this report, castor oil based hyperbranched polyester/bitumen modified fly ash nanocomposites were fabricated to achieve the desired performance. The hyperbranched polyester resin was synthesized by a three-step one pot condensation reaction using monoglyceride of castor oil based carboxyl terminated pre-polymer and 2,2-bis (hydroxymethyl) propionic acid. Also, the bulk fly ash of paper industry waste was converted to hydrophilic nano fly ash by ultrasonication followed by transforming it to an organonano fly ash by the modification with bitumen. The synthesized polyester resin and its nanocomposites were characterized by different analytical and spectroscopic tools. The nanocomposite obtained in presence of 20 wt% styrene (with respect to polyester) was found to be more homogeneous and stable compared to nanocomposite without styrene. The performance in terms of tensile strength, impact resistance, scratch hardness, chemical resistance and thermal stability was found to be improved significantly after formation of nanocomposite compared to the pristine system after curing with bisphenol-A based epoxy and poly(amido amine). The overall results of transmission electron microscopic (TEM) analysis and performance showed good exfoliation of the nano fly ash in the polyester matrix. Thus the studied nanocomposites would open up a new avenue on development of low cost high performing surface coating materials.

Effects of Al-Surplus and Ca-Deficiency on Content and Rhythm of ATP in Plant Leaves (식물엽의 ATP함량과 그 주기에 미치는 Al과잉 및 Ca결핍의 영향)

  • 성민웅
    • Journal of Plant Biology
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    • v.23 no.1
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    • pp.17-25
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    • 1980
  • Kidneybean(Phaseolus vlgaris L.) and buckwheat(Fagopyrum esculentum M$\"{O}$nch) seedlings cultured with Hoagland solution to the height of 7 to 10cm in the earthern pot containing sand were used for experimental plants. One group of the plants was irrigated with Hoagland solution composed of various Al concentrations of 0 to 1,000 ppm containing 50ppm Ca, and the other group was irrigated with Hoagland solution composed of various Ca concentrations of 0 to 1,000ppm containing 100ppm Al for a month during June and July. By Al-surplus over 100ppm, the margins of backwheat leaves were curied down and turned into yellow-brown necrosis simialr to Ca-deficiency, and the old kidney bean leaves showed marginal and veinal chlorosis in addition to the early shedding of leaves. With Al-surplus of 100ppm the content of chlorophyll a and b in both plants was decreased by 30~40%. The ratio 3/1 of chlorophyll a/b was not altered by Al-surplus and Ca-deficienty at an early stage, but it was affected markedly at a final stage of growth. Leaf respiration with 100ppm Al containing 50pm Ca was slightly affected in buckwheat, but decreased by 20% in kidneybean. With Al-surplus of 100ppm the ATP content was decreased by 32% in the kidneybean leaves and by 80% in buckwheat leaves, whereas with Ca-deficiency it was decreased by 72% in kidneybean and by 90% in buckwheat. The rhythm of ATP level showed self-relience without being affected by light or temperature under the green house condition although by Al-s rplus and Ca-deficiency the ATP level of the rhythm was lower than that of control.trol.

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Isolation and Characterization of Plant Growth Promoting Rhizobacteria from Waste Mushroom bed from Agaricus bisporus (양송이 수확 후 배지로부터 식물생장촉진세균의 분리 및 생육특성)

  • Jung, Young-Pil;Kyung, Ki-Cheon;Jang, Kab-Yeul;Yoon, Min-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.5
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    • pp.866-871
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    • 2011
  • An auxin-producing bacteria (3YN11-02) was isolated from waste mushroom bed from Agaricus bisporus of Chungnam Buyeo-Gun area. The strain 3YN11-02 was identified as a novel species belongs to Rahnella aquatica by a chemotaxanomic and phylogenetic nalysis. The isolate was confirmed to produce indole-3-acetic acid (IAA) which is one of auxin hormone by TLC and HPLC analysis. When the concentration of IAA was assessed by performing HPLC quantity analysis, the maximal $290mg\;L^{-1}$ of IAA detected in ether fraction extracted from the culture filtrate which was cultured in R2A broth containing 0.1% tryptophan for 24 h at $35^{\circ}C$. The molecular weight of the main peak obtained by LC-mass analysis was correspondent well to 175, that of IAA. To investigate the growth promoting effect of crop, when the culture broth of R. aquatica 3YN11-02 was infected onto water culture and seed pot of mung bean, the adventitious root induction and root growth of mung bean were 2.0 times higher than control.

A New Cymbidium Orchid Variety "Daegook" bred by In Vitro Mutagenesis (조직배양 돌연변이 기술에 의한 Cymbidium 난 품종 "대국" 육종)

  • Kang, Kyung-Won;Park, Kwang-Seob;Mo, Sug Youn;Kim, Doo Hwan;Kang, Si-Yong
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.510-514
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    • 2009
  • Cymbidium is one of the largest genus in the orchid family and a number of hybrids have been bred in the world. During mass-propagating the Cymbidium "Dongyang" using the meristem culture technology, a useful leaf mutant was selected from the protocom like bodies. The new Cymbidium variety by in vitro mutangesis from "Dongyang" was named as 'Daegook' in 1998. Compared to Dongyang, "Daegook" mutant has white or yellow stripes along the margin of leaves and flowers. The plant length of "Daegook" was shorter than "Dongyang" and the mean length and width of leaf in "Daegook" was 40 cm and 1.6 cm, respectively. The new variety, "Daegook", is expected to be a popular Cymbidium variety among consumer as a ornamental orchid mutant for pot culture by its fine and unique stripes and growth characters.

Frequency analysis of storm surge using Poisson-Generalized Pareto distribution (Poisson-Generalized Pareto 분포를 이용한 폭풍해일 빈도해석)

  • Kim, Tae-Jeong;Kwon, Hyun-Han;Shin, Young-Seok
    • Journal of Korea Water Resources Association
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    • v.52 no.3
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    • pp.173-185
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    • 2019
  • The Korean Peninsula is considered as one of the most typhoon related disaster prone areas. In particular, the potential risk of flooding in coastal areas would be greater when storm surge and heavy rainfall occurred at the same time. In this context, understanding the mechanism of the interactions between them and estimating the risk associated with the concurrent occurrence are of particular interests especially in low-lying coastal areas. In this study, we developed a Poisson-Generalized Pareto (Poisson-GP) distribution based storm surge frequency analysis model to combine the occurrence of the exceedance of a threshold, that is the peaks over threshold (POT), within a Bayesian framework. The storm surge frequency analysis technique developed through this study might contribute to the improvement of disaster prevention technology related to storm surge in the coastal area.