• 농업과학연구
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• 제19권2호
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• pp.269-272
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• 1992

A new single glutinous maize hybrid "Huin Chal 1" was developed at the Genetics and Breeding Lab., Dept. of Agron., College of Agriculture, Chungnam National University. Parents for the hybrid were all derived from Korean indigenous maize lines which were collected in 1980s. One of the parental inbred lines was originated from Bosung, Chonnam province and other inbred line was from Jewon, Chungbuk province. Both inbreds were developed by ear-to-row selection method through selling and named as Bosung and Jewon, respectively. Performace tests for the hybrid which was produced between Bosung and Jewon have been conducted five times including one test at Crop Experiment Station, Rural Development Administration. From the previous performance tests, the hybrid was recognized as high yielding hybrid with good agronomic characteristics and we named the hybrid as Huin Chal 1., and hereby we report the agronomic characters of the newly developed hybrid.

• Fisheries and Aquatic Sciences
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• 제18권1호
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• pp.99-107
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• 2015

A natural hybrid of a probable intergeneric mating between the striped shiner Pungtungia herzi and the stone morocco Pseudorasbora parva (Cypriniformes: Cyprinidae) was captured in the Geumho River, a tributary of the Nakdong River basin in Korea. Morphological characters and DNA sequences were analyzed to verify its hybrid state and identify the parentage of its parent species. The hybrid exhibited a phenotypic intermediacy between the two parent species in the number of vertebrae and the mouth shape. Out of 1,488 base pair (bp) positions of the nuclear recombination activating gene 1 gene (rag1), which has a biparental mode of inheritance, 41-bp substitutions were detected between the two parent species, whereas an electropherogram of the hybrid displayed polymorphic double peaks at all of the base positions, along with one additional one, strongly indicating its hybrid state. Meanwhile, sequence comparison of the mitochondrial cytochrome b gene (mt-cyb) (1,140 bp), which has a maternal mode of inheritance, showed only 5-22-bp differences (97.6-99.5% identities) between the hybrid and Pu. herzi, but as many as 158-168-bp differences (85.2-86.1% identities) between the hybrid and Ps. parva, clearly indicating Pu. herzi as the maternal species. Thus, combined analyses of independent data sets (i.e., morphology and nuclear and mitochondrial DNA sequences) offered convincing evidence for the hybrid state of a naturally occurring hybrid resulting from intergeneric mating between a female Pu. herzi and a male Ps. parva.

• 산업경영시스템학회지
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• 제39권4호
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• pp.60-66
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• 2016

Compound logistics is a service aimed to enhance logistics efficiency by supporting that shippers and consigners jointly use logistics facilities. Many of these services have taken place both domestically and internationally, but the joint logistics services for e-commerce have not been spread yet, since the number of the parcels that the consigners transact business is usually small. As one of meaningful ways to improve utilization of compound logistics, we propose a brokerage service for shipper and consigners based on the hybrid recommendation system using very well-known classification and clustering methods. The existing recommendation system has drawn a relatively low satisfaction as it brought about one-to-one matches between consignors and logistics vendors in that such matching constrains choice range of the users to one-to-one matching each other. However, the implemented hybrid recommendation system based brokerage agent service system can provide multiple choice options to mutual users with descending ranks, which is a result of the recommendation considering transaction preferences of the users. In addition, we applied feature selection methods in order to avoid inducing a meaningless large size recommendation model and reduce a simple model. Finally, we implemented the hybrid recommendation system based brokerage agent service system that shippers and consigners can join, which is the system having capability previously described functions such as feature selection and recommendation. As a result, it turns out that the proposed hybrid recommendation based brokerage service system showed the enhanced efficiency with respect to logistics management, compared to the existing one by reporting two round simulation results.

• 동아시아식생활학회지
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• 제13권3호
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• pp.185-190
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• 2003

재래종 돼지고기는 우리 고유의 돈육으로서 보존의 의미가 있을 뿐만 아니라 최근 고기의 육질과 맛에 대한 소비자의 기호가 증가하는 추세이다. 본 연구는 재래종과 개량종 돼지고기의 등, 배, 다리의 세 부분을 시료로 하여 몇 가지의 영양성분 함량을 조사하여 품질을 비교하고자 하였으며 그 결과는 다음과 같다. 1 일반성분 중 수분 함량은 60.82%~72.08%로서 재래종에 비하여 개량종에서 많았으며, 단백질 함량은 재래종의 등 부위에서 19.71%로서 가장 많았고, 개량종의 다리부위에서 13.14%로서 가장 적었다. 조 지방 함량은 개량종의 배 부위에서 34.44%로 가장 많았고, 개량종의 다리부위에서는 2.33%로서 가장 적었으며, 탄수화물함량은 재래종의 배 부위가 13.28%로 가장 많았고, 회분함량은 재래종이 개량종보다 많았다. 2. 무기성분 중 Mg, Fe의 함량은 모든 부위에서 재래종이 개량종보다 많았으며 특히 Fe이 현저하였다. K과Na은 그다지 차이가 적었으나 P은 재래종 다리부위에서 개량종보다 많았다. 3. 총 유리아미노산의 함량은 부위별로는 다리부위가 많았고 재래종(12.27mg)이 개량종(11.22mg)에서보다 많았다. 유리아미노산 조성 중 감칠맛 성분 함량은 재래종의 다리부위에서 개량종보다 약 1.8배 이상 많았으며, 단맛 성분도 재래종의 등 부위에서 가장 많았다. 4 콜레스테롤 함량은 재래종의 등, 배 부위에서 개량종보다 적었으나 다리부위는 재래종(789.32$\mu\textrm{g}$)이 개량종 (538.84$\mu\textrm{g}$)보다 많았다(p< .01).

• Journal of Life Science
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• 제9권2호
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• pp.52-56
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• 1999

The Raf, a cytoplasmic serine/thereonine protein kinase, acts as an important mediator of signals involving cell proliferation, differentiation and development. Multiple regulatory elements should participate in the expression of D-raf, Drosophila homolog of human c-raf-1. In order to search regulatory factors involved in the D-raf promoter activation, we accomplished the yeast one-hybrid screening using D-raf promoter region from bp-330 to -309 with respect to the transcription initiation site as bait. After screening, sixteen independent positive clones of ${\beta}$-galactosidase activties were identified and sequenced. Two clones having 94-98% identity with daughterless and one clone having 93% identity with escargot by Blast search among these clones were screened.

• Animal cells and systems
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• 제6권4호
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• pp.317-322
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• 2002

To improve conventional yeast one-hybrid screening, we have developed an efficient mammalian one-hybrid system that allows rapid isolation of com-plementary DNAs which are able to induce human p14$^{ARF}$. tumor suppressor gene. A 1.5 kb promoter region of p14$^{ARF}$ was fused to EGFP to generate ARF promoter-EGFP reporter vector. This reporter plasmid was stably trans-fected into NIH3T3 cells for generation of reporter cell line. When the reporter cell line was infected with E2F-1 together with excess amounts of empty vector, the cells that received the positive modulator were readily identifiable by green fluorescence using FACS. The GFP-positive cells were cloned directly from the cultured cells and expanded in bulk culture. The genomic DNAs from GFP-positive cells were prepared and the CDNA insert in integrated retroviral genome was recovered by PCR using primers annealing to the retroviral vector sequences flanking the insert-cloning site. This system should be useful for efficient screening of expression CDNA libraries in mammalian cells to identify novel upstream regulators for spe-cific genes by one-hybrid interaction.ion.

• 한국잠사곤충학회지
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• 제40권1호
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• pp.27-32
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• 1998

One indigenous polyvoltine strain (Nistari) and two bivoltine strains viz. P5 and NB18 along with one bivoltine hybrid (P5$\times$NB18) were subjected for studies on the differences on some physiologically important biomolecules like protein, nucleic acids and cholesterol. Ovarian protein and RNA content remained significantly high in the bivoltine races and their hybrid over multivoltine breed, while, DNA and cholesterol content remained significantly low in all the breeds and the hybrid as compared to Nistari strain. However, the ovarian weight was higher in both the bivoltine breeds and their hybrid than that of Nistari. Higher ovarian weight together with more protein and RNA concentrations reflect the preparatory phase for production of diapausing eggs by the bivoltine silknoths and their hybrid. The variations in biochemical parameters studied herein, thus establish a distinct difference in the overt reproductive physioiogy between multivoltine and bivoltine silkworms.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1996년도 한국자동제어학술회의논문집(국내학술편); 포항공과대학교, 포항; 24-26 Oct. 1996
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• pp.743-746
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• 1996

A hybrid control scheme to regulate the force and position by dual arms is proposed, where two arms are treated as one arm in a kinematic viewpoint. Our approach is different from other hybrid control approaches which consider robot dynamics, in the sense that we employ a purely kinematic based approach for hybrid control, with regard to the nature of position-controlled industrial robots. The proposed scheme is applied to sawing task. In the sawing task, the trajectory of the saw grasped by dual arms is planned in an offline fashion. When the trajectory of the saw is planned to follow a line in a horizontal plane, 3 position parameters are to be controlled(i.e, two translational positions and one rotational position). And a certain level of contact force has to be controlled along the vertical direction(i.e., minus z-direction) not to loose the contact with the object to be sawn. Typical feature of sawing task is that the contact position where the force control is to be performed is continuously changing. Therefore, the kinematic mapping between the force controlled position and the joint actuators has to be updated continuously. The effectiveness of the proposed control scheme is experimentally demonstrated. The proposed hybrid control scheme can be applied to arbitrary dual arm systems, regardless of their kinematic structure and the number of actuated joints.

• 대한화학회지
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• 제55권2호
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• pp.256-261
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• 2011

Bis acetylated hybrid pyrazoles 을 합성하여 이들 화합물에 대해 녹는점, 원소분석, MS, FT-IR, one-dimensional $^1H,-$ 및 $^{13}C$-NMR로 분석하였다. 합성한 화합물들에 대해 in vitro 항균활성을 Candida sp. namely Candida albicans, Candida glabrata, Candida parapsilosis, Candida dubliniensis 및 Candida tropicali 균에 대해 수행하였다. Pyrazoles의 페닐고리에 작용기($-CH_3$, $-OCH_3$, -F, -Cl, 및 Br)가 있는 화합물은 Candida species에 대해서 강한 활성을 나타내었다.

• Corrosion Science and Technology
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• 제16권2호
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• pp.85-89
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• 2017

One of the most challenging issues in the oil and gas industry is corrosion assessment and management in subsea structures or equipment. At present, almost all steel pipelines are sensitive to corrosion in harsh working environments, particularly in salty water and sulphur ingress media. Nowadays, the most commonly practiced solution for a damaged steel pipe is to entirely remove the pipe, to remove only a localized damaged section and then replace it with a new one, or to cover it with a steel patch through welding, respectively. Numerous literatures have shown that fiber-reinforced polymer-based composites can be effectively used for steel pipe repairs. Considerable research has also been carried out on the repair of corroded and gouged pipes incorporated with hybrid natural fiber-reinforced composite wraps. Currently, further research in the field should focus on enhanced use of the lesser and highly explored hybrid-biocomposite material for the development in corrosion prevention. A hybrid-biocomposite material from renewable resource based derivatives is cost-effective, abundantly available, biodegradable, and an environmentally benign alternative for corrosion prevention. The aim of this article is to provide a comprehensive review and to bridge the gap by developing a new hybrid-biocomposite with superhydrophobic surfaces.