• Title/Summary/Keyword: One-hybrid

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Deconstructionism in Issey Miyake's Fashion Design

  • Kwon, Hae-Sook
    • Journal of Fashion Business
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    • v.11 no.6
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    • pp.87-100
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    • 2007
  • This study aims at analyzing the characteristics of deconestructionism expressed in Issey Myake's fashion who is one of the most creative designers in the world. Method and contents of this study are as follows. Through the review of literatures, three major categories such as interminacy of meaning, decentring, and intertextuality and eight subcategories have been identified as major features of deconestructionism. These subcategories for content analysis were selected to examine the characteristics of deconstructionism expressed in Issey Myake's design in depth. Interminacy of meaning was divided into unstructured and unconstructed factors while decentring into qualities of cross-gender, subculture, anti-fashion, and post-humanism. Lastly, intertextuality was classified into factors of disordered mixing mode and mixed styles. Materials for analysis were chosen from total 349 designs on the website,www.firstview.com, which carries Issey Miyake's collection from 2002 S/S through 2006 F/W. 2 fashion major analysts including the researcher participated in 1st analysis and two another fashion major analysts participated in 2nd analysis. The final level of consistency between analysts was over 97% for all categories. The data analysis quantitatively evaluates the frequency of each category and qualitatively evaluates the characteristics of design. The result is as follows. First, the avant-garde facets of inter-textuality are most prominent. In particular, Miyake has introduced the various novelly textiles to fashion and employed the mixtured styles, not to copy them but to create hybrid designs by using pastiches and mixing fashion styles. Moreover, he has used various fabrics in order to inflict shock and contrast but still create subtle harmony, thus enriching his fashion. Second, the indeterminacy of meaning is the second most frequently shown facet of Miyake's fashion design. Miyake has employed unconstructed expression techniques, which has projected unusual images free from basic forms of designs and also has used the unstructured styles which dismantle harmony and balance-the common rule of design. He has made a new attempt to acknowledge clothing as an expressive artwork by allowing clothes to take a complete form when a person actually wears them. He has demonstrated a unique perspective on cloth construction and presented a love for artistic collaboration in the development of his collections A distinct manufactured aesthetic is exaggerated, even hyperbolic. Thirdly, the aspects of decentralization were also shown in Miyake's design, however, it has been visibly decreased after 2002 S/S.

A ubiquitin-proteasome system as a determination factor involved in methylmercury toxicity

  • Hwang, Gi-Wook
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2006.11a
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    • pp.46-54
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    • 2006
  • The methylmercury (MeHg) is a toxic environmental pollutant, causing serious neurological and developmental effects in humans. Recent epidemiological studies have indicated that ingestion of MeHg in fish during pregnancy can result in neuroethological effects in the offspring. However, the mechanism underlying the MeHg-toxicity is not fully understood. To elucidate the mechanisms of toxicity of MeHg and of defense against MeHg, we searched for factors that determine the sensitivity of yeast cells to MeHg, and found that overexpression of Cdc34, a ubiquitin-conjugating enzyme (E2) that is a component of the ubiquitin-proteasome (UP) system, induces a resistance to MeHg toxicity in both yeast and human cells. The UP system is involved in the intracellular degradation of proteins. When Cdc34 is overexpressed in cells, ubiquitination reactions are activated and the degradation of certain proteins by the UP system is enhanced. Therefore, it seems likely that certain as-yet-unidentified proteins that increase MeHg toxicity might exist in cons and that toxicity might be reduced by the enhanced degradation of such proteins, mediated by the UP system, when Cdc34 is overexpressed. SCF ubiquitin-ligase is a component of UP system and consists of Skpl, the scaffold protein Cdc53, the RING-finger protein Hrt1, and one member of the family of F-box proteins. The F-box proteins directly bind to the substrates and are the determinants of substrate specificity of SCF. Therefore, we searched for the f-box protein that cofers resistance to MeHg, and found that overexpression of Hrt3 or Yi1224w induced resistance to MeHg toxicity in yeast cells. Since the protein(5) that enhance toxicity of MeHg might plausibly be induced in substrates of both f-box proteins, we next searched for substrate proteins that are recognized by Hrt3 or Y1r224w using two-hybrid screen. We found that Did3 or Crsl interacts with Hrt3; and Eno2 interacts with Yir224w. The yeast cells that overexpressed each those proteins showed hypersensitivity to MeHg, respectively, indicating that those proteins enhance the MeHg toxicity. Both Dld3 and Eno2 are proteins involved in the synthesis of pyruvate, and overexpression of both proteins might induce increase in interacellular levels of pyruvate. Deletion of Yi1006w that transports pyruvate into the mitochondria induced aresistance to MeHg. These results suggest that the promotion of the pyruvate irdlowinto the mitochondria might enhance MeHg toxicity. This study providesimportant keyfor the elucidauon of the molecular mechanism of MeHg toxicity.

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Factors Affecting Efficiency of Anther Culture and Phenotypic Characteristics of Anther -derived Progeny in Broccoli (녹색꽃양배추 (Brassica oleracea L. var. italica)의 약배양 효율증진과 약유래 계통의 특성)

  • 남시춘;윤광현;백기엽
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.3
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    • pp.189-195
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    • 1999
  • This study was carried out to clarify several factors affecting embryogenesis from anther culture of nine cultivars in Brassica oleracea L. var. italica and to investigate the characteristics of plants derived from anther culture. Androgenesis from anther culture was elevated on the B5 medium supplemented with 0.1mg/L NAA, 0.1mg/L 2.4-D and 10% sucrose. Embryo production in liquid medium was five-fold higher than solid medium. High temperature treatment at 35$^{\circ}C$ for one day before transfer to culture room maintained at $25^{\circ}C$ had effective to induce embryogenesis of cultured anthers but extended treatment at 35$^{\circ}C$ decreased significantly the percent of embryogenesis. Frequency of embryogenesis from cultured anthers exhibited significant difference from 2.8% in 'Green Valiant' to 21% in 'Haisi' as affected by genotypes. Percent of spontaneously dihaploid among regenerated plants from anther culture was ranged from 62 to 74% as affected by the genotypes. Characteristic in relation to plant height, number of leaves and branches, and size of head from anther-derived plants showed differential variation in 'Rokguray' and 'Haisi'. Among these charaters obtained from two cultivars, five lines were selected for early maturity, long plant height and large head. Selected lines were used as breeding meterials for F$_1$ hybrid.

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Transformation of Populus alba $\times$Populus glandulosa Using Phosphinothricin Acetyltransferase Gene (Phosphinothricin acetyltransferase 유전자를 이용한 현사시의 형질전환)

  • 오경은;양덕춘;문흥규;박재인
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.3
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    • pp.163-169
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    • 1999
  • This study was conducted to produce herbicide resistant plants by transferring phosphinothricin acetyltransferase (PAT) gene into Populus alba $\times$ Populus glandulosa No .3 using Agrobacterium tumefaciens MP 90/PAT. Leaf segments from in vitro grown shoots of hybrid poplar No. 3 were soaked in a AB medium containing Agrobacterium tumefaciens MP 90/PAT for 10 min and cocultivated for 2 days on MS medium containing 1.0 mg/L 2,4-D and 0.2mg/L kinetin (CIM). Putative transformed calli could be selected after cocultivation of leaf segments on CIM supplemented with 50mg/L kanamycin and 500mg/L cefotaxime for 3 weeks. The selected calli were cultured on CIM supplemented with 50 mg/L kanamycin and 500 mg/L cefotaxime for 5~8 weeks before transfer to WPM containing 1.0mg/L zeatin, 0.1mg/L BAP, 50 mg/L kanamycin and 500mg/L cefotaxime for shoot regeneration. Shoots were regenerated from the callus after 4 week cultivation, and the regenerants were grown on the same medium for 7~l0 weeks. The plants rooted on 1/2 WPM containing 0.2 mg/L IBA and 50 mg/L kanamycin. To confirm the gene insertion into plants, GUS activity was detected by histochemical assay in the transformed plants. Finally, the presence of both NPT II and PAT genes from the transgenic plants were confirmed by PCR amplification with the gene specific primers and subsequent PCR-Southern blot with DIG-labeled PAT gene probe. After acclimatization in pots for 4 weeks, the plants were sprayed by 3 mL/L of Basta to test resistance to the herbicide. The transgenic plants remained green, whereas all the control plants died after one week.

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Interface of Tele-Task Operation for Automated Cultivation of Watermelon in Greenhouse

  • Kim, S.C.;Hwang, H.
    • Journal of Biosystems Engineering
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    • v.28 no.6
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    • pp.511-516
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    • 2003
  • Computer vision technology has been utilized as one of the most powerful tools to automate various agricultural operations. Though it has demonstrated successful results in various applications, the current status of technology is still for behind the human's capability typically for the unstructured and variable task environment. In this paper, a man-machine interactive hybrid decision-making system which utilized a concept of tole-operation was proposed to overcome limitations of computer image processing and cognitive capability. Tasks of greenhouse watermelon cultivation such as pruning, watering, pesticide application, and harvest require identification of target object. Identifying water-melons including position data from the field image is very difficult because of the ambiguity among stems, leaves, shades. and fruits, especially when watermelon is covered partly by leaves or stems. Watermelon identification from the cultivation field image transmitted by wireless was selected to realize the proposed concept. The system was designed such that operator(farmer), computer, and machinery share their roles utilizing their maximum merits to accomplish given tasks successfully. And the developed system was composed of the image monitoring and task control module, wireless remote image acquisition and data transmission module, and man-machine interface module. Once task was selected from the task control and monitoring module, the analog signal of the color image of the field was captured and transmitted to the host computer using R.F. module by wireless. Operator communicated with computer through touch screen interface. And then a sequence of algorithms to identify the location and size of the watermelon was performed based on the local image processing. And the system showed practical and feasible way of automation for the volatile bio-production process.

Actor's Role and Networks in the Environmentally Friendly Farming in Busan Metropolitan Agricultural Region (부산 김해평야 농업지역 친환경농업의 행위자-연결망 연구)

  • Kim, Ki-Hyuk
    • Journal of the Korean association of regional geographers
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    • v.9 no.3
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    • pp.276-296
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    • 2003
  • This study is to analyze the adoption of environmentally friendly fanning(EFF) by Actor-Network Theory(ANT). ANT declares that the world is full of hybrid entities containing both human and non-human elements and maintains that adoption of an innovation comes as a consequence of the action of everyone in the chain of actors who has anything to do with it. In this study, adoption of EFF will be analysed through the role of actors and networks. And this paper try to identify the intermediaries and obligatory passage point(OPP) in each networks. In study area, 5 actors, -nature, governmental institute, food processors, consumers and farmers-, have each roles in their networks, But only 18 farmers adopted EFF. This study revealed that three OPPs were not overcome in each network. The one is nature, such as water and soil pollution. Another is shortage of reliability between farmers and governmental institute. The other is shortage of information about agricultural commodity trade. And through this application of ANT to the EFF, we contend that ANT can be useful for studies of diffusion of EFF and sustainability of rural systems in situations where interactions of the social, technological and political are regarded as particularly important.

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Development of Hybrid Rice Facilitated by Cytoplasmic Genetic Male Sterility II. Responses of Korean Cultivars to the Chinese Cytoplasmic Genetic Male Sterile lines (웅성불임성을 이용한 수도잡종품종개발 II. 중공의 세포형질 유전적 웅성불임계통에 대한 몇가지 한국품종의 반응)

  • 허문회;김홍열;조윤희
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.29 no.3
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    • pp.227-231
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    • 1984
  • Chinese CGMS (Cytoplasmic-Genetic Male Sterility) is being transfered to Korean cultivars which will be used as CGMS maintainers. These maintainer lines along with the Chinese CGMS lines and their maintainer lines were crossed with another group of Korean cultivars which will be used as the fertility restorers, and their yield heterosis were examined. The yield heterosis was calculated as high as 113.0-221.1 % in terms of heterosis (F$_1$/Midparent) with the grand mean of 150.5%, and, 86.1-179.8% in terms of heterobeltiosis (F$_1$/better parent) with the grand mean of 125.3%. Among the female parent groups, Chinese mainteiners were the highest following by Chinese MS lines and the Korean maintainers were the lowest. Heterobeltiosis of yield components were 92.7%, 120.0%, 103.0% and 112.0% for number of panicles/unit area, number of grains/panicle, fertility per cent and 1000 grain weight, respectively. Path-coefficients of yield components to the yield were calculated. The highest one was number of grains per panicle ranging 0.8073-0.8649 followed by the 1000 grain weight ranging 0.2000-0.5032. The grain yield ratios to the grand mean were calculated in terms of combining ability. It was highest with the Chinese maintainers followed by Chinese MS lines and The Korean maintainers were lowest. This facts bring cautions to the breeding efforts to transfer the Chinese CGMS in to Korean maintainers.

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Genetic Distance and Heterosis Degree Among the Developed Waxy Corn Lines (찰옥수수 육성계통간 유전적 거리와 잡종강세 정도)

  • Lee, Moon-Sub;Yang, Jae-Hyeon;Lim, Seung-Bin;Lee, Hee-Bong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.4
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    • pp.464-469
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    • 2015
  • This study was carried out to find relationships of genetic distance and heterosis expression degree in the developed CNU waxy corn lines. The used material were developed in Corn Breeding Laboratory, Coll. of Agri. & Life Sci., CNU. Total 10 primers used for SSR maker analysis. In genetic distance, the used lines divided into four groups; A group has two lines, B group has one line, C group has ten lines and D group has eleven lines, respectively. Among used lines, CNU427 and CNU588 were very closed as a 74, while CNU451 and CNU429 were remote as a 40 inbred coefficient, respectively. While heterosis degree were very variable not only hybrids but also cross parents. Average heterosis of most cross-parent was high in plant height, ear height, flowering day and ear length. Especially, CNU H09-23 hybrid was high as 79.4% than other hybrids. Here, we gained the fact has closely relationship between genetic distance and heterosis.

First Report of a Hybridization between Caretta caretta and Chelonia mydas from Jeju Island, South Korea (한국의 제주에서 발견된 바다거북 잡종(Caretta caretta ${\times}$ Chelonia mydas)에 대한 첫 보고)

  • Koo, Kyo-Soung;Han, Sang-Hyun;Oh, Hong-Shik
    • Korean Journal of Environmental Biology
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    • v.32 no.4
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    • pp.377-381
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    • 2014
  • In this study, we report for the first time a hybridization between Caretta caretta and Chelonia mydas in South Korea. On 7th May 2012, a sea turtle (Jeju sea turtle 01, JST01) was found in the east coast of Jeju Island, South Korea. The morphological characteristics of JST01 were compared with those of C. caretta and C. mydas that were frequently observed in Korean Seas. Interestingly, JST01 showed similar morphological characters such as body color, appearance of carapace, shape of upper beak, serration on marginal scute, and two claws with those of C. caretta. On the other hand, JST01 showed also similar patterns in several characteristics including the numbers of marginal, inframarginal and costal scute, smooth carapace without keel, no contact between costal scute and nuchal scute with those of C. mydas. From these results, we suggest that JST01 is one of hybrid animals bred between C. caretta and C. mydas, because JST01 has species-specific characters from both species. We anticipate that our study would be useful and have significant impact onto study inter-specific hybridization in the groups of sea turtle.

A Contents-based Drug Image Retrieval System Using Shape Classification and Color Information (모양분류와 컬러정보를 이용한 내용기반 약 영상 검색 시스템)

  • Chun, Jun-Chul;Kim, Dong-Sun
    • Journal of Internet Computing and Services
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    • v.12 no.6
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    • pp.117-128
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    • 2011
  • In this paper, we present a novel approach for contents-based medication image retrieval from a medication image database using the shape classification and color information of the medication. One major problem in developing a contents-based drug image retrieval system is there are too many similar images in shape and color and it makes difficult to identify any specific medication by a single feature of the drug image. To resolve such difficulty in identifying images, we propose a hybrid approach to retrieve a medication image based on shape and color features of the medication. In the first phase of the proposed method we classify the medications by shape of the images. In the second phase, we identify them by color matching between a query image and preclassified images in the first phase. For the shape classification, the shape signature, which is unique shape descriptor of the medication, is extracted from the boundary of the medication. Once images are classified by the shape signature, Hue and Saturation(HS) color model is used to retrieve a most similarly matched medication image from the classified database images with the query image. The proposed system is designed and developed especially for specific population- seniors to browse medication images by using visual information of the medication in a feasible fashion. The experiment shows the proposed automatic image retrieval system is reliable and convenient to identify the medication images.