• BMB Reports
• /
• 제28권4호
• /
• pp.281-289
• /
• 1995

Homogeneous human deoxycytidine kinase was purified in one step from a variety of spontaneous human leukemic cells (T-ALL, B-ALL, B-CLL, AML, CML), and from cultured T-lymphoblast cells (MOLT-4) using the newly developed affinity medium, $dCp_4$-Sepharose. Starting with an ammonium sulfate fraction, purification was achieved in one step with the kinase being eluted from a column by the end product inhibitor, dCTP. The purified deoxycytidine kinase from T-ALL cells phosphorylated deoxyadenosine and deoxyguanosine, as well as deoxycytidine. The enzyme purified from T-ALL and B-CLL cells yielded one major band with a molecular weight of 52 kDa determined by SDS-polyacrylamide gel electrophoresis. AML and CML cells yielded one 52 kDa band and an extra band of 30 kDa molecular weight. On the other hand, B-ALL and MOLT-4 cells showed a low molecular weight band of 30 kDa only. However, the electrophoretic mobilities of enzymatic activity in 12% non-denaturing gels were identical for the dCyd kinase from all different kinds of leukemic cell lines, except that the B-ALL, B-CLL, and MOLT-4 cell preparations had an extra minor peak, all at the same position. dAdo and dCyd phosphorylating activities comigrated indicating that these activities are all associated with the same protein. Two new methods, a disk implantation method and a nitrocellulose powder method were used with a small amount of enzyme protein to raise polyclonal antibodies against dCyd kinase purified from T-ALL cells.

• 한국의류학회지
• /
• 제26권10호
• /
• pp.1467-1477
• /
• 2002

This study was investigated physical properties of stretch fabrics by KES-FB system to show suitable basic data to making skirts of excellent capacity and develop more organized basic skirt pattern by fitness evaluation. 1. The results of T. H. V.(Total Hand Value) were as followa: In the kind of blending fiber, cotton/spandex was more excellent than nylon/spandex and polyester/spandex, in the direction of stretch, one-way(weft inserted polyurethan yarn) polyester/spandex and cotton/spandex were higher than two-way(warp and wet inserted polyurethan yarn) polyester/spandes and cotton/spandex, two-way nylon/spandex was higher than one-way nylon/spandex. 2. The results of calculating the variance between sample stretch fabrics and Japanese s/s women's suit fabrics after standardizing were as follows: Stretch fabrics has 2 range of tensile, bending, shearing, compression, surface, thickness and weight as compared with Jpanese s/s women's suit fabrics. In the tensile property, one-way stretch fabrics were almost the same with Japanese s/s women's suit fabrics, only two-way polyester/spandex had+1～+2 range. In the bending, shear property, there was no difference between sample fabrics and Japanese s/s women's suit fabrics 3. In the total fitness of the skirt, nylon/spandex is the best in the fabrics and one-way stretch fabric is better than two-way strethch fabric.

• 보건행정학회지
• /
• 제9권3호
• /
• pp.149-169
• /
• 1999

A purpose of this study is to understand Platonic View of Health in $\boxDr$Politeia$\boxUl$. Though Plato was not so much a doctor as a philosopher. he had health care of children at heart. He mapped out an ideal type of nation in $\ulcorner$Politeia$\lrcorner$. and he founded a Akademeia in order to realize his dreams. In his course of education. he put emphasis on the problem of health. He extended poetry education for mental health and physical education for physical health. He placed high value on mental health above physical health. and poetry education corresponds to our reading education of today. He perceived that reading had a considerable influence on mental health promotion. According to his assertion, life style, too. had something to do with health condition. To lead a simple. temperate life makes one' health promote, on the other hand, to lead a complicated, intemperate life makes one' health injure. Morever, he approved of a eugenic marriage and the law of jungle. If one is unable to take care of one' health oneself. he would rather die than live. We cannot accept this proposal by general consent. but we cannot be too careful of our health. We can draw out a philosophy of health from Platonic View of Health. For example. the importance of health education. the preference of mental health. the influence of reading education. and responsibility for self-care, etc. We need to establish a philosophy of health scientifically by lasting study of records.

• 제어로봇시스템학회논문지
• /
• 제19권4호
• /
• pp.322-327
• /
• 2013

Recently many people have been troubled with perennial allergic rhinitis. But there is no way to cure for root of it until now. Therefore many medical treatments to release symptoms of perennial allergic rhinitis have been researched. One of them is localized aerosol hyperthermia, which injects $42^{\circ}C{\sim}43^{\circ}C$ vapor into nasal cavity. Vapor inhalator for localized aerosol hyperthermia has been researched in foreign country. But on the other hand it has rarely been researched inside of the country. Since most clinics and hospitals have used imported vapor inhalator, it is needed to develop domestic vapor inhalator. In the paper, a superior vapor inhalator compared to former developed one is developed. The superiority of it comes from direct-vapor heating instead of water heating to control vapor temperature. The developed vapor inhalator has shorter rising time than the existing one because of direct-vapor heating. Furthermore vapor generation part and control part of the vapor inhalator developed as one piece mock-up. It enables the vapor inhalator to have smaller size. Many laboratory tests are performed and compared to existing results to prove its performance.

• 한국전산유체공학회:학술대회논문집
• /
• 한국전산유체공학회 2007년도 춘계 학술대회논문집
• /
• pp.72-78
• /
• 2007

To develop the aerodynamic performance, there are widely two group of studies are achieved. The first one is about design of the vehicles geometry and the second one is about aerodynamic devices. Geometry design is highly credible and stable method. But it is not flexible and each parts are related interactively. So if one part geometry are modified, the other parts are required to be redesigned. The other hand, flow control by aerodynamic device is flexible and modulized method. Though it needs energy, relatively little input makes far advanced aerodynamic performance. Synthetic Jet is one of the second group method. The device repeats suction and blowing motion in constant frequency. According to the performance, the flow which are near the flight surface are served momentum. This mechanism can reduce the aerodynamic loss by boundary layer and separated flow. Synthetic jet actuator has several parameters, that influence the flow control. This study focus the parameters effects of the synthetic jet - orifice geometry, frequency, jet speed and etc.

• 한국의류산업학회지
• /
• 제12권1호
• /
• pp.94-102
• /
• 2010

The dyeing of poly(trimethylene terephthalate)(PTT)/silk mixture fabrics can be accomplished by a two bath dyeing method with separate application of the disperse dyes on the PTT, reduction cleaning of the stained silk and then dyeing the silk with the acid dyes, or by one bath dyeing method with mixed dye ranges, possibly followed by a cleaning treatment. The two bath dyeing method has the advantage of better results with respect to dry cleaning fastness properties thanks to the possibility of an intermediate reduction clear. On the other hand, as compared with the two bath dyeing method, one bath dyeing method with a mixed dye range permits rapid and more reproducible dyeing, without the risk of great difference with respect to the shade of the strike on both substrates as well as savings of time, energy and water usage. This study was carried out to investigate dyeing characteristics of PTT/silk mixture fabrics with disperse dyes/acid dyes by one bath dyeing method in comparison with two bath dyeing method in the interests for rationalization of the dyeing process.

• 한국자동차공학회논문집
• /
• 제14권3호
• /
• pp.142-149
• /
• 2006

A PIV(Particle Image Velocimetry) was applied to measure in-cylinder velocity field according to inlet valve angle during intake stroke. Two engines, one is conventional DOHC 4 valve and the other is narrow valve angle, were used to compare real intake flow. The results show that the intake flow pattern of conventional engine is more complicated than that of narrow angle one in horizontal plane and the vertical component of in-cylinder flow is rapidly decayed at the end stage of intake. On the other hand, the flow pattern of narrow angle one is relatively well arranged in horizontal plane and the vertical velocity component remains so strongly that forms large-scale strong tumble. Two engines also form commonly three tumble; two are small and bellow the intake valve and one is large-scale. The center of large scale tumble moves to bottom of cylinder as the vertical velocity increases.

• 한국의상디자인학회지
• /
• 제2권2호
• /
• pp.55-79
• /
• 2000

This study is the design research for the practical use of Casual Hanbok. The conclusion of basic research for Casual Hanbok design reads as follows. Today's college women are fairly positive to the practical use of Casual Hanbok. Especially, they are strongly interested in the new design and have the active intention to wear it on actually. The concrete design of every parts of Casual Hanbok which is wanted by college women is as follows. Firstly, college women like well matched two-piece dress style in colors. And they want to decide the coloration independently than to follow the traditional coloration passively. Secondly, according to the design of jacket, sometimes they like the presence of a collar, a collar strip, a gore etc. and at other times they like absence of them. Thirdly, the side line of jacket they like and the seam line of sleeve they like is smoothly curved one and the adjusting means they like is a fancy button. Fourthly, the length of jacket they like reaches to their waist and the width of an armhole they like must be convenient for moving of arm. Fifthly, one out of two college women likes mixed spinning fabrics as material for jacket, skirt, pants. In the other hand, one out of five college women likes cotton. Sixthly, the skirt style they like is A ­line one having from two to four gathers on the waist and the length of skirt they like reaches to their ankle or calf of the leg. Seventhly, the pants style they like is the western style but the hem line of pants is the korean traditional pants style.

• Journal of Power Electronics
• /
• 제4권2호
• /
• pp.102-111
• /
• 2004

This paper is concerned with a deadbeat current control implementation of shunt-type three-phase active power filter (APF). Although the one-dimensional deadbeat control method can attain time-optimal response of APF compensating current, one sampling period is actually required fur its settling time. This delay is a serious drawback for this control technique. To cancel such a delay and one more delay caused by DSP execution time, the desired APF compensating current has to be predicted two sampling periods ahead. Therefore an adaptive predictor is adopted for the purpose of both predicting the control error of two sampling periods ahead and bringing the robustness to the deadbeat current control system. By adding the adaptive predictor output as an adjustment term to the reference value of half a source voltage period before, settling time is made short in a transient state. On the other hand, in a steady state, THD (total harmonic distortion) of the utility grid side AC source current can be reduced as much as possible, compared to the case that ideal identification of controlled system could be made.

• 한국산학기술학회논문지
• /
• 제15권7호
• /
• pp.4132-4138
• /
• 2014

청소년의 건강증진을 위한 보건교육 프로그램 중 손씻기에 대한 교육을 기획하는데 있어 기초자료를 제공하고자 2011년, 2012년, 2013년의 청소년건강행태온라인조사 원시자료를 이용하여, 중학생 및 고등학생의 손 씻기 실천 행태를 분석하였다. 자료 분석은 조사에 참여한 표본이 우리나라 청소년의 대표성을 갖도록 지정된 가중치를 적용하였으며, 자료는 SPSS 18.0 프로그램을 이용하여 빈도 분석 및 교차분석을 실시하였다. 우리나라 청소년의 손 씻기 실천 행태는 학교에서 식사하기 전 '전혀 씻지 않았다'로 응답한 학생수가 2011년도에 22,257명(29.4%), 2012년도 22,375명(30.5%), 2013년도 13.153(18.5%)명으로 다른 항목과 달리 계속적으로 주의깊게 관찰해야 하는 상태로 유지되었다. '학교에서 식사하기 전 전혀 씻지 않았다'는 항목에 대해 최근 3개년간의 추이를 확인한 결과 도시구분에서는 대도시와 중소도시가 군단위의 소도시 보다 유의하게 전혀 손을 씻지 않는 경향을 보였고, 학교유형에서는 여학교가 남녀 공학이나 남학교 보다 학교에서 식사 전 전혀 손을 씻지 않았다고 응답한 비율이 지난 3개년 간 계속해서 높은 경향을 보였다. 또한 학교구분에서는 중학교와 일반 고등학교가 특성화 고교보다, 성별에서는 여학생이 남학생보다, 학년 구분에서는 고학년으로 학년이 올라갈수록 '학교에서 식사 전 전혀 손을 씻지 않았다'고 응답한 학생이 유의하게 많은 것으로 나타났다. 이로써 손 씻기에 대한 학교 보건 교육 시 '식사 전 손 씻기'에 대한 교육을 특히 강조해야 하고, 학교에서 식사 전 '전혀 손을 씻지 않았다'고 응답한 비율이 높았던 인구학적 특성의 대상에 대해 보다 세심한 교육과 계속적인 관찰이 요구된다.