• Title/Summary/Keyword: One-hand

Search Result 5,350, Processing Time 0.03 seconds

Purification of Deoxycytidine Kinase from Various Human Leukemic Cells by End-product Analog Affinity Chromatography

  • Kim, Min-Young
    • BMB Reports
    • /
    • v.28 no.4
    • /
    • pp.281-289
    • /
    • 1995
  • Homogeneous human deoxycytidine kinase was purified in one step from a variety of spontaneous human leukemic cells (T-ALL, B-ALL, B-CLL, AML, CML), and from cultured T-lymphoblast cells (MOLT-4) using the newly developed affinity medium, $dCp_4$-Sepharose. Starting with an ammonium sulfate fraction, purification was achieved in one step with the kinase being eluted from a column by the end product inhibitor, dCTP. The purified deoxycytidine kinase from T-ALL cells phosphorylated deoxyadenosine and deoxyguanosine, as well as deoxycytidine. The enzyme purified from T-ALL and B-CLL cells yielded one major band with a molecular weight of 52 kDa determined by SDS-polyacrylamide gel electrophoresis. AML and CML cells yielded one 52 kDa band and an extra band of 30 kDa molecular weight. On the other hand, B-ALL and MOLT-4 cells showed a low molecular weight band of 30 kDa only. However, the electrophoretic mobilities of enzymatic activity in 12% non-denaturing gels were identical for the dCyd kinase from all different kinds of leukemic cell lines, except that the B-ALL, B-CLL, and MOLT-4 cell preparations had an extra minor peak, all at the same position. dAdo and dCyd phosphorylating activities comigrated indicating that these activities are all associated with the same protein. Two new methods, a disk implantation method and a nitrocellulose powder method were used with a small amount of enzyme protein to raise polyclonal antibodies against dCyd kinase purified from T-ALL cells.

  • PDF

Fitness and Physical Properties in Current Stretch Fabrics for Bottoms -Focused on the Tight Skirt- (하의용 시판 신축성 소재의 물리적 특성과 맞음새에 과한 연구 -스커트를 중심으로-)

  • 이진희;최혜선;도월희
    • Journal of the Korean Society of Clothing and Textiles
    • /
    • v.26 no.10
    • /
    • pp.1467-1477
    • /
    • 2002
  • This study was investigated physical properties of stretch fabrics by KES-FB system to show suitable basic data to making skirts of excellent capacity and develop more organized basic skirt pattern by fitness evaluation. 1. The results of T. H. V.(Total Hand Value) were as followa: In the kind of blending fiber, cotton/spandex was more excellent than nylon/spandex and polyester/spandex, in the direction of stretch, one-way(weft inserted polyurethan yarn) polyester/spandex and cotton/spandex were higher than two-way(warp and wet inserted polyurethan yarn) polyester/spandes and cotton/spandex, two-way nylon/spandex was higher than one-way nylon/spandex. 2. The results of calculating the variance between sample stretch fabrics and Japanese s/s women's suit fabrics after standardizing were as follows: Stretch fabrics has 2 range of tensile, bending, shearing, compression, surface, thickness and weight as compared with Jpanese s/s women's suit fabrics. In the tensile property, one-way stretch fabrics were almost the same with Japanese s/s women's suit fabrics, only two-way polyester/spandex had+1~+2 range. In the bending, shear property, there was no difference between sample fabrics and Japanese s/s women's suit fabrics 3. In the total fitness of the skirt, nylon/spandex is the best in the fabrics and one-way stretch fabric is better than two-way strethch fabric.

A Study on Platonic View of Health in "Politeia" (플라톤의 건강관에 대한 고찰 -"국가"를 중심으로 -)

  • 반덕진
    • Health Policy and Management
    • /
    • v.9 no.3
    • /
    • pp.149-169
    • /
    • 1999
  • A purpose of this study is to understand Platonic View of Health in $\boxDr$Politeia$\boxUl$. Though Plato was not so much a doctor as a philosopher. he had health care of children at heart. He mapped out an ideal type of nation in $\ulcorner$Politeia$\lrcorner$. and he founded a Akademeia in order to realize his dreams. In his course of education. he put emphasis on the problem of health. He extended poetry education for mental health and physical education for physical health. He placed high value on mental health above physical health. and poetry education corresponds to our reading education of today. He perceived that reading had a considerable influence on mental health promotion. According to his assertion, life style, too. had something to do with health condition. To lead a simple. temperate life makes one' health promote, on the other hand, to lead a complicated, intemperate life makes one' health injure. Morever, he approved of a eugenic marriage and the law of jungle. If one is unable to take care of one' health oneself. he would rather die than live. We cannot accept this proposal by general consent. but we cannot be too careful of our health. We can draw out a philosophy of health from Platonic View of Health. For example. the importance of health education. the preference of mental health. the influence of reading education. and responsibility for self-care, etc. We need to establish a philosophy of health scientifically by lasting study of records.

  • PDF

Development of a Direct-Vapor Heating Inhalator (증기직접가열방식의 비염치료기 개발)

  • Jeon, Chang-Wan;Kang, Taesam
    • Journal of Institute of Control, Robotics and Systems
    • /
    • v.19 no.4
    • /
    • pp.322-327
    • /
    • 2013
  • Recently many people have been troubled with perennial allergic rhinitis. But there is no way to cure for root of it until now. Therefore many medical treatments to release symptoms of perennial allergic rhinitis have been researched. One of them is localized aerosol hyperthermia, which injects $42^{\circ}C{\sim}43^{\circ}C$ vapor into nasal cavity. Vapor inhalator for localized aerosol hyperthermia has been researched in foreign country. But on the other hand it has rarely been researched inside of the country. Since most clinics and hospitals have used imported vapor inhalator, it is needed to develop domestic vapor inhalator. In the paper, a superior vapor inhalator compared to former developed one is developed. The superiority of it comes from direct-vapor heating instead of water heating to control vapor temperature. The developed vapor inhalator has shorter rising time than the existing one because of direct-vapor heating. Furthermore vapor generation part and control part of the vapor inhalator developed as one piece mock-up. It enables the vapor inhalator to have smaller size. Many laboratory tests are performed and compared to existing results to prove its performance.

STUDY OF FLOW CONTROL CHARACTER USING SYNTHETIC JET (Synthetic jet을 이용한 유동제어 특성연구)

  • Hong, Woo-Ram;Kim, Sang-Hoon;Kim, Woo-Re;Kim, Yu-Shin;Kim, Chong-Am
    • 한국전산유체공학회:학술대회논문집
    • /
    • 2007.04a
    • /
    • pp.72-78
    • /
    • 2007
  • To develop the aerodynamic performance, there are widely two group of studies are achieved. The first one is about design of the vehicles geometry and the second one is about aerodynamic devices. Geometry design is highly credible and stable method. But it is not flexible and each parts are related interactively. So if one part geometry are modified, the other parts are required to be redesigned. The other hand, flow control by aerodynamic device is flexible and modulized method. Though it needs energy, relatively little input makes far advanced aerodynamic performance. Synthetic Jet is one of the second group method. The device repeats suction and blowing motion in constant frequency. According to the performance, the flow which are near the flight surface are served momentum. This mechanism can reduce the aerodynamic loss by boundary layer and separated flow. Synthetic jet actuator has several parameters, that influence the flow control. This study focus the parameters effects of the synthetic jet - orifice geometry, frequency, jet speed and etc.

  • PDF

A Study on the Dyeing of PTT(polytrimethylene terephthalate)/Silk Mixture Fabrics with Disperse Dyes/Acid Dyes (분산염료/산성염료에 의한 PTT(polytrimethylene terephthalate)/견 교직물의 염색에 관한 연구)

  • Sung, Woo-Kyung
    • Fashion & Textile Research Journal
    • /
    • v.12 no.1
    • /
    • pp.94-102
    • /
    • 2010
  • The dyeing of poly(trimethylene terephthalate)(PTT)/silk mixture fabrics can be accomplished by a two bath dyeing method with separate application of the disperse dyes on the PTT, reduction cleaning of the stained silk and then dyeing the silk with the acid dyes, or by one bath dyeing method with mixed dye ranges, possibly followed by a cleaning treatment. The two bath dyeing method has the advantage of better results with respect to dry cleaning fastness properties thanks to the possibility of an intermediate reduction clear. On the other hand, as compared with the two bath dyeing method, one bath dyeing method with a mixed dye range permits rapid and more reproducible dyeing, without the risk of great difference with respect to the shade of the strike on both substrates as well as savings of time, energy and water usage. This study was carried out to investigate dyeing characteristics of PTT/silk mixture fabrics with disperse dyes/acid dyes by one bath dyeing method in comparison with two bath dyeing method in the interests for rationalization of the dyeing process.

In-Cylinder Intake Flow Characteristics according to Inlet Valve Angle (흡입 밸브 각도에 따른 실린더 내 흡입 유동 특성 비교)

  • Ohm, In-Yong;Pak, Chan-Jun
    • Transactions of the Korean Society of Automotive Engineers
    • /
    • v.14 no.3
    • /
    • pp.142-149
    • /
    • 2006
  • A PIV(Particle Image Velocimetry) was applied to measure in-cylinder velocity field according to inlet valve angle during intake stroke. Two engines, one is conventional DOHC 4 valve and the other is narrow valve angle, were used to compare real intake flow. The results show that the intake flow pattern of conventional engine is more complicated than that of narrow angle one in horizontal plane and the vertical component of in-cylinder flow is rapidly decayed at the end stage of intake. On the other hand, the flow pattern of narrow angle one is relatively well arranged in horizontal plane and the vertical velocity component remains so strongly that forms large-scale strong tumble. Two engines also form commonly three tumble; two are small and bellow the intake valve and one is large-scale. The center of large scale tumble moves to bottom of cylinder as the vertical velocity increases.

A Design Research for the Practical Use of College Women′s Casual Hanbok (여대생들의 생활한복 실용화를 위한 디자인 연구)

  • 김장향
    • Journal of the Korea Fashion and Costume Design Association
    • /
    • v.2 no.2
    • /
    • pp.55-79
    • /
    • 2000
  • This study is the design research for the practical use of Casual Hanbok. The conclusion of basic research for Casual Hanbok design reads as follows. Today's college women are fairly positive to the practical use of Casual Hanbok. Especially, they are strongly interested in the new design and have the active intention to wear it on actually. The concrete design of every parts of Casual Hanbok which is wanted by college women is as follows. Firstly, college women like well matched two-piece dress style in colors. And they want to decide the coloration independently than to follow the traditional coloration passively. Secondly, according to the design of jacket, sometimes they like the presence of a collar, a collar strip, a gore etc. and at other times they like absence of them. Thirdly, the side line of jacket they like and the seam line of sleeve they like is smoothly curved one and the adjusting means they like is a fancy button. Fourthly, the length of jacket they like reaches to their waist and the width of an armhole they like must be convenient for moving of arm. Fifthly, one out of two college women likes mixed spinning fabrics as material for jacket, skirt, pants. In the other hand, one out of five college women likes cotton. Sixthly, the skirt style they like is A ­line one having from two to four gathers on the waist and the length of skirt they like reaches to their ankle or calf of the leg. Seventhly, the pants style they like is the western style but the hem line of pants is the korean traditional pants style.

  • PDF

Robust Deadbeat Current Control Method for Three-Phase Voltage-Source Active Power Filter

  • Nishida, Katsumi;Ahmed, Tarek;Nakaoka, Mutsuo
    • Journal of Power Electronics
    • /
    • v.4 no.2
    • /
    • pp.102-111
    • /
    • 2004
  • This paper is concerned with a deadbeat current control implementation of shunt-type three-phase active power filter (APF). Although the one-dimensional deadbeat control method can attain time-optimal response of APF compensating current, one sampling period is actually required fur its settling time. This delay is a serious drawback for this control technique. To cancel such a delay and one more delay caused by DSP execution time, the desired APF compensating current has to be predicted two sampling periods ahead. Therefore an adaptive predictor is adopted for the purpose of both predicting the control error of two sampling periods ahead and bringing the robustness to the deadbeat current control system. By adding the adaptive predictor output as an adjustment term to the reference value of half a source voltage period before, settling time is made short in a transient state. On the other hand, in a steady state, THD (total harmonic distortion) of the utility grid side AC source current can be reduced as much as possible, compared to the case that ideal identification of controlled system could be made.

Behaviors of hand washing practice Korean adolescents, 2011-2013: The Korea Youth Risk Behavior Web-based Survey (청소년의 손 씻기 실천 행태 분석; 청소년 건강행태 온라인 조사 2011-2013년도를 중심으로)

  • Choi, Young-Sil
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.15 no.7
    • /
    • pp.4132-4138
    • /
    • 2014
  • The purpose of this assessment was to provide the basic data for setting up education in terms of 'Hand wash' as one of the health plan & education programs for adolescents. The task analyzed the behavior of students regarding hand washing, which were ranged from middle school to high school. The SPSS 18.0 statistical program, frequency-test and cross-analysis were used for data analysis by 2011, 2012 and 2013, which were the recent 3 years, the Korea Youth Risk Behavior Web-based Survey data. In the data, the response of "Never washed" from students before having a meal accounted for 29.4% in 2011, 30.5% in 2012 and 18.5% in 2013, respectively. Unlike other subjects, these facts suggest that this kind of behavior should be considered significant under the assessment. By tracking the trend over three years, some facts were confirmed in that students living in the metropolitan and medium-sized cities were less likely to wash their hands than students in small- sized towns. In terms of gender, female students were less likely to wash their hands than male students. Regarding the type of school, more students in the public middle & high schools had a tendency to respond "Never hand wash" than the students in the special-purpose high schools. Furthermore, as the grade was increased in middle school and high school, students were less likely to wash their hands before meals in school. Therefore, Health promotion and health education for students should be conducted more carefully with more emphasis on this point.