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Mechanism Underlying NaF-Induced Apoptosis in Human Oral Squamous Cell Carcinoma

  • Hur, Young-Joo;Kim, Do-Kyun;Lee, Seung-Eun;Kim, In-Ryoung;Jeong, Na-Young;Kim, Ji-Young;Park, Bong-Soo
    • International Journal of Oral Biology
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    • v.35 no.2
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    • pp.51-60
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    • 2010
  • Few studies have evaluated the apoptosis-inducing efficacy of NaF on cancer cells in vitro but there has been no previous investigation of the apoptotic effects of NaF on human oral squamous cell carcinoma cells. In this study, we have investigated the mechanisms underlying the apoptotic response to NaF treatment in the YD9 human squamous cell carcinoma cell line. The viability of YD9 cells and their growth inhibition were assessed by MTT and clonogenic assays, respectively. Hoechst staining, DNA electrophoresis and TUNEL staining were conducted to detect apoptosis. YD9 cells were treated with NaF, and western blotting, immunocytochemistry, confocal microscopy, FACScan flow cytometry, and MMP and proteasome activity assays were performed sequentially. The NaF treatment resulted in a time- and dose-dependent decrease in YD9 cell viability, a dose-dependent inhibition of cell growth, and the induction of apoptotic cell death. The apoptotic response of these cells was manifested by nuclear condensation, DNA fragmentation, the reduction of MMP and proteasome activity, a decreased DNA content, the release of cytochrome c into the cytosol, the translocation of AIF and DFF40 (CAD) into the nucleus, a significant shift of the Bax/Bcl-2 ratio, and the activation of caspase-9, caspase-3, PARP, Lamin A/C and DFF45 (ICAD). Furthermore, NaF treatment resulted in the downregulation of G1 cell cyclerelated proteins, and upregulation of p53 and the Cdk inhibitor $p27^{KIP1}$. Taken collectively, our present findings demonstrate that NaF strongly inhibits YD9 cell proliferation by modulating the expression of G1 cell cycle-related proteins and inducing apoptosis via mitochondrial and caspase pathways.

An Implementation of QR Code based On-line Mobile Augmented Reality System (QR코드 기반의 온라인 모바일 증강현실 시스템의 구현)

  • Park, Min-Woo;Park, Jung-Pil;Jung, Soon-Ki
    • Journal of Korea Multimedia Society
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    • v.15 no.8
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    • pp.1004-1016
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    • 2012
  • This paper proposes a mobile augmented reality system to provide detail information of the products using QR code included in them. In the proposed system, we perform the estimation of the camera pose using both of marker-based and markerless-based methods. If the camera can see the QR code, we perform the estimation of the camera pose using the set of rectangles in the QR code. However, if the QR code is out of sight, we perform the estimation of the camera pose based homography between consecutive frames. Moreover, the content of the augmented reality in the proposed system is made by using meta-data. Therefore, the user can make contents of various scenario using only meta-data file without modification of our system. Especially, our system maintains the contents as newly updated state by the on-line server. Thus, it can reduce the unnecessary update of the program.

Preparation of Refuse-Derived-Fuel from Automobile Shredder Residues (폐자동차 파쇄 잔류물을 이용한 고분연료의 제조연구)

  • Lee Hwa-Yaung;Oh Jong-Kee;Kim Sung-Gyu
    • Resources Recycling
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    • v.12 no.4
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    • pp.58-64
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    • 2003
  • A study on the preparation of RDF with ASR materials from shredder line of end-of-life vehicles has been peformed by using hot molding method. ASR materials used in the work were plastics, fibers and sponge which were cut by less than 10 mm in size prior to hot molding and 40, 60 and 80 mm mold in diameter were employed for RDF forming. It was found that heating temperature, heating time and mixing ratio of materials played an important role in the hot molding of ASR materials. Density of RDF samples Prepared in the work was in the range of 0.5∼0.9gr/ml depending on the hot molding conditions and it was decreased with increase in the content of sponge. Breaking strength of RDF samples was observed to 0.8∼3.0MPa and it was also increased with increase in density of RDF samples. Finally, when heating temperature was elevated, density of RDF was increased due to Increase in molten part of ASR materials which resulted in the enhancement of breaking strength of RDF.

Comic Image Normalization using the gradient Radon Transform based on OpenCL implementation (OpenCL 기반의 그래디언트 라돈변환을 이용한 만화영상의 정규화)

  • Kim, Dong-Keun;Jeon, Hyeok-June;Hwang, Chi-Jung
    • The KIPS Transactions:PartB
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    • v.18B no.4
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    • pp.221-230
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    • 2011
  • Digital comic images are one of popular contents on the Internet. Usually, they are scanned from comic books by digital scanners. Without post-processing, they may have different sizes, skews and margins other than contents at the boundary. To normalize the size of their contents without the skews and margins is an important step in comic image analysis and application such as content-based comic image retrieval system. In this paper, we propose a method to detect a box frame in comic images by extracting of line segments using the gradient Radon transform. The box frame in comic images is the maximum rectangle which consists of contents without margins. We use the detected box frame to normalize the size of comic images and to make them no skew. In addition, the proposed method is implemented by OpenCL to speed up the detection of the line segments. Experimental results show that our proposed method effectively detects the box frame in comic images.

A Study on Knowledge based Conference Management System Architecture (지식기반 회의관리 시스템 아키텍처에 관한 연구)

  • Kim Tae-Hyun;Song Jeong-Chul;Kim Chang-Su;Song Jeong-Young;Kim Jin-Soo;Jung Hoe-Kyung
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2006.05a
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    • pp.939-942
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    • 2006
  • Currently, There is a conference system in one field of knowledge base system, the study against a conference of multimedia approach method is become accomplished actively. However, Case of the conference system which is studied from multimedia viewpoint, only it will bring the merit and demerit of the off-line conference of existing with on-line, it isn't considering to make knowledge plan of important conference content and method. This thesis proposes stand of Knowledge-base system architecture for it break from the method which composes a conference system from the multimedia side of existing and focus it will be able to contain existing systems to conference contents and method.

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Consumers’Purchasing Process of Fashion Products on the Internet: A Qualitative Approach (인터넷을 통한 패션상품 구매행동의 탐색적 연구)

  • 김현정;이은영;박재옥
    • Journal of the Korean Society of Clothing and Textiles
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    • v.24 no.6
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    • pp.907-917
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    • 2000
  • Although interest in the potential and actual usage of the Internet as a transaction medium is increasing, the market for fashion merchandise on the Internet in Korea has yet to take off. As the Internet environment is expected to bring about a transformation of conventional consumer buying behavior, the purpose of this exploratory research is to investigate the buying behavior of fashion products on the Internet to identify relevant concepts and generate hypotheses for further empirical research. The research methods selected for the study were observation and in-depth interview. Twelve subjects who had purchased fashion products on the Internet were selected and interviewed. Those who could not participate in face-to-face in-depth interviews because of the geographic locations were interviewed on-line. The results are as follows: First, subjects went through the stages of shopping motivation stage, site choice behavior stage, in-site behavior stage, and postpurchase behavior stage. Second, a model was extracted for each shopping stage, and a final model was completed based on comparisons with the participants processes. The information content of each phase was discussed. Finally, each participant was classified using their purchasing process, revealing six possible mixed usage patterns of the Internet marketing system and the traditional marketing system.

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A Study on the Communication Relationship Structure and Expression Methods in Interior Design - Focused on the Practical design process of communication - (인테리어 디자인에서 커뮤니케이션 관계구조와 표현방법에 관한 연구 - 실무 디자인 프로세스 커뮤니케이션 중심으로 -)

  • Seo, Ji-Hye;Hong, Il-Tae
    • Korean Institute of Interior Design Journal
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    • v.22 no.5
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    • pp.199-206
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    • 2013
  • Design is one of diverse human communication activities. Development of technologies has led to execution of more active design communication functions, stirring social and cultural changes. The concept of design communication has become stronger by overcoming the limitations of verbal communication and expanding the methods of communication. These social changes are highlighted In the design of modern space. Even though communication in interior design activities is so important, detailed studies on communication of each entity are still very insufficient. Design communication refers to tools and activities for overall communications in the design process. In design activities, relevant communication is indispensible. Therefore, studies on practical communication methods are essential for accurate communication of content that has to be shared in the results or in the process of obtaining the results, rather than only focusing on the future techniques and functions of design. In other words, improving the efficiency of interior design communication requires establishing a communication relationship structure of each entity, which calls for proper expression methods depending on each entity. Therefore, this study is aimed at exploring efficient expression methods in line with the relationship structure of each entity in the interior design process.

Agronomic and Genetic Evaluation on a Dull Mutant Line Derived from the Sodium Azide Treated 'Namil', a Non-Glutinous Japonica Rice (남일벼 돌연변이 유래 중간찰 계통의 작물학적 특성 및 배유특성 지배유전자위 표지)

  • Chun, Jae-Buhm;Jeung, Ji-Ung;Cho, Seong-Woo;Kim, Woo-Jae;Ha, Ki-Young;Kang, Kyung-Ho;Ko, Jae-Kwon;Kim, Hyun-Soon;Kim, Bo-Kyeong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.4
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    • pp.448-457
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    • 2015
  • Developing rice lines with various amylose contents is necessary to diverse usages of rice in terms of raw materials for processed food production, and thereby to promote rice consumption in Korea. A rice mutant line, 'Namil(SA)-dull1' was established through sodium azide mutagenesis on 'Namil', a non-glutinous Korean Japonica rice cultivar. Namil(SA)-dull1' had dull endosperm characteristics and the evaluated amylose content was 12.2%. A total of 94 F2 progenies from a cross between 'Namil(SA)-dull1' and 'Milyang23', a non-glutinous Tongil-type rice cultivar, was used for genetic studies on the endosperm amylose content. Association analyses, between marker genotypes of 53 SSR anchor markers and evaluated amylose contents of each 94 F2:3 seeds, initially localized rice chromosome 6 as the harboring place for the modified allele(s) directing low amylose content of 'Namil(SA)-dull1'. By increasing SSR marker density on the putative chromosomal region followed by association analyses, the target region was narrowed down 0.94 Mbp segment, expanding from 28.95 Mbp to 29.89 Mbp, on rice chromosome 6 pseudomolecule. Among the SSR loci, RM7555 explained 84.2% of total variation of amylose contents in the $F_2$ population. Further physical mapping on the target region directing low amylose content of 'Namil(SA)-dull1' would increase the breeding efficiency in developing promising rice cultivars with various endosperm characteristics.

Geochemical exploration for REE occurrence in Nghe An Area within Northern Vietnam (베트남 북부 네안 희토류 산출지의 지구화학탐사)

  • Heo, Chul-Ho;Chung, Ho Tien;Lee, Jae-Ho
    • Economic and Environmental Geology
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    • v.45 no.6
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    • pp.599-622
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    • 2012
  • The phase I soil geochemical exploration was carried out targeting around Chau Binh area far from about 14 km with southeastern direction from Quy Chau within Nghe An province. The interval of sampling are horizontal 300 m with 14 line and longitudinal 500 m with 15 line, resulting in 194 soil samples. Based on the result of the phase I soil geochemical exploration, the phase II detailed pitting survey was carried out targeting the grid point with high TREO content, resulting in 56 soil samples within 7 pits. The geology of survey area are consisted of Ban Chieng biotite granite complex and Dai Loc gneissic granite complex intruding Bu Khang formation comprising of schist, gneiss and limestone. Main mineralization in the study area have the characteristics of occurrence with tin, ruby and REE-bearing monazite(about 300 g/t) and xenotime(about 10 g/t) to be thought as occurring at the alteration zone of granite complex. In order to elucidate the source rock of monazite and xenotime confirmed from heavy sand, soil geochemical exploration was carried out. As a analysis result with ICP-MS on the soil samples from the phase I soil geochemical exploration, total REE oxide content of background amount to about 2 times of crustal abundance, enriching the heavy rare earth(about 2 times) and light rare earth(about 1.84 times). As a analysis result with ICP-MS on the soil samples from the phase II soil detailed pit survey, we identified outcrop considering as economic ore body at the grid point 4-7 pit with N40W attitude. As a synthetic consideration on the phase I soil geochemical exploration and phase II detailed pit survey, we tentatively designated areas considering as the extension of economic ore body with REE anomaly. In the near future, we have the plan to carry out the geophysical exploration and test drilling targeting the interval anticipated to the economic ore body.

Geochemical Exploration for Tri Le REE Occurrence in Nghe An Province within Northern Vietnam (베트남 북부 네안성 칠레 희토류 산출지의 지구화학탐사)

  • Heo, Chul-Ho;Ho, Tien Chung;Lee, Jae-Ho
    • Economic and Environmental Geology
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    • v.47 no.2
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    • pp.147-168
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    • 2014
  • The soil geochemical exploration was carried out targeting around Tri Le area far from about 30 km with northwestern direction from Que Phong within Nghe An province. The interval of sampling are horizontal 200 m interval with 23 line and longitudinal 300 m with 10 line, resulting in 228 soil samples. Based on the result of the soil geochemical exploration, the detailed pitting survey was carried out targeting the grid point with high TREO content, resulting in 75 soil samples within 7 pits. The geology of survey area are consisted of Ban Chieng biotite granite complex and granitic gneiss intruding Ban Khang formation comprising of quartz schist and marble. Main mineralization in the study area have the characteristics of occurrence with tin, ruby and REE-bearing monazite and xenotime to be thought as occurring at the alteration zone of granite complex. In order to elucidate the source rock of monazite and xenotime confirmed from heavy sand, soil geochemical exploration was carried out. As a analysis result with ICP-MS on the soil samples from the soil geochemical exploration, total REE oxide content of background amount to about 2 times of crustal abundance, enriching the heavy rare earth(about 2 times) and light rare earth(about 1.5 times). As a analysis result with ICP-MS on the soil samples from the soil detailed pit survey, we only identified outcrop considering as economic weathered granite body at the grid point 1-10 pit among 7 pits. As a synthetic consideration on the soil geochemical exploration and detailed pit survey, we tentatively designated Tri Le area as no promising target for REE. In 2014, we have the plan to carry out the soil geochemical exploration targeting the extended economic REE ore body in Quy Chau as project area from 2011 to 2012.