• Animal cells and systems
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• 제3권3호
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• pp.259-267
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• 1999

The expression of the neural cell adhesion molecule-180 (NCAM-180), which accumulates at contact sites between cells and may be responsible for the stabilization of cell contacts, was studied in the olfactory system and retina of developing and adult rats. From embryonic day 12 onwards, which was the earliest stage examined, the NCAM-180 pathway directing to the presumptive olfactory bulb was observed. In later stages, olfactory neurons and fasciculating axons in the olfactory epithelium and nerve fiber layer and glomeruli of the olfactory bulb expressed NCAM-180. From postnatal day 0, immunolabelling pattern of the olfactory epithelium and olfactory bulb were the same as that during later stages. NCAM-180 immunoreactivity was present on differentiating retinal cells and persisted on those cells throughout adulthood. However, contrary to the olfactory nerve which remained detectable in the adult, the optic nerve was only transiently expressed with NCAM-180 and was no longer detectable in the adult. The presence of NCAM-180 in olfactory tissues suggests their possible role in pathfinding, differentiation, fasciculation and synaptic plasticity. The continued presence of NCAM-180 in the olfactory system examined may underlie its continuous cell turnover and regenerative capacity. The continuous expression of NCAM-180 in ganglion cells, bipolar cells and photoreceptor cells, also suggests potential regenerating capability and some plastic functions for these cells in the adult. Since the expression of NCAM-180 by the optic nerve was restricted to the period of special histogenetic events, for example, during axonal growth and synaptogenesis, it is possible that the lack of NCAM-180 in the adult optic nerve might cause a nonpermissive environment for the regeneration and result in regenerative failure of this system.

• Korean Journal of Otorhinolaryngology-Head and Neck Surgery
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• 제61권11호
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• pp.567-572
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• 2018

The sensory function of the trigeminal nerve is to provide tactile, proprioceptive, and nociceptive afference by chemical stimulation. Various physical responses of the trigeminal nerve to stimuli help to defend against harmful substances. Recently, many studies have been conducted on solitary chemoreceptor cells innervated by trigeminal nerve. Most volatile organic compounds stimulate both the olfactory and trigeminal nervous systems. In general, the trigeminal nervous system is less sensitive than the olfactory nervous system. Studies have shown that sensation of the trigeminal nerve by chemical stimulation results in inhibition of olfaction. This indicates that the olfactory and trigeminal nerves interact with each other in the central nervous system. It is important to study various noxious stimuli acting on the trigeminal nerve in modern society where environmental concerns are intensifying.

• Journal of Korean Neurosurgical Society
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• 제45권2호
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• pp.103-106
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• 2009

Intracranial schwannomas preferentially arise from the vestibular branch of the eighth nerve, and rarely from the trigeminal nerve, facial nerve, and lower cranial nerves. Anterior cranial fossa schwannomas are extremely uncommon and few details about them have been reported. The patient was a 39-year-old woman whose chief complaints were anosmia and frontal headache for 2 years. The gadolinium (Gd)-enhanced magnetic resonance imaging (MRI) showed an extra-axial mass from ethmoid sinus to right frontal base region near the midline, with solid enhancement in lower portion and multicystic formation in upper portion. The tumor was totally resected via basal subfrontal approach. At operation, the tumor had cystic portion with marginal calcification and the anterior skull base was destructed by the tumor. The olfactory bulb was involved, and the tumor capsule did not contain neoplastic cells. The histopathological diagnosis was schwannoma. We report a rare case of anterior cranial fossa schwannoma with literature review.

• 대한수의학회지
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• 제39권3호
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• pp.407-416
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• 1999

This study was carried out to investigate the NPY-immunohistochemical characteristics of the olfactory bulb in the striped field mouse(Apodemus agrarius). The animals were anesthesized with thiopental sodium and perfused with 4% paraformaldehyde through left ventricle and aorta. Brains were removed and tranfered 10%, 20% and 30% sucrose. Sections were then cut on a cryostat into $40{\mu}m$-thick. The tissue immunostained with avidin-biotinylated complex method. The main olfactory bulb consisted of seven circumferential laminae : an olfactory nerve fiber layer, a glomerular layer with glomeruli surrounding by periglomerular cells, an external plexiform layer having granule and tufted cells, a mitral cell layer, a narrow internal plexiform layer, a granule cell layer forming several cell rows and a layer of white matter. The accessory olfactory bulb had four layers : an olfactory or vomeronasal nerve fiber layer, a glomerular layer consisting of small glomeruli, a mixed layer not distinguishing the external plexiform/mitral cell/granule cell layers and a granule cell layer. Most of NPY-immunoreactive(NPY-IR) neurons in main olfactory bulb were localized in the deeper portion of granule cell layer, white matter and anterior olfactory nucleus. In addition, some NPY-IR neurons were identified in the external plexiform layer. The shape of NPY-IR neurons of all olfactory bulb were predominant round or oval, sometime multipolar in shape. And most NPY-IR processes were parallel to long axis of white matter. In accessory olfactory bulb, NPY-IR neurons were not found in all region.

• 대한두개안면성형외과학회지
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• 제18권2호
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• pp.92-96
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• 2017

Background: All nasal bone fractures have the potential for worsening of olfactory function. However, few studies have studied the olfactory outcomes following reduction of nasal bone fractures. This study evaluates posttraumatic olfactory dysfunction in patients with nasal bone fracture before and after closed reduction. Methods: A prospective study was conducted for all patients presenting with nasal bone fracture (n=97). Each patient consenting to the study underwent the Korean version of Sniffin' Sticks test (KVSS II) before operation and at 6 month after closed reduction. The nasal fractures were divided according to the nasal bone fracture classification by Haug and Prather (Types I-IV). The olfactory scores were compared across fracture types and between preoperative and postoperative settings. Results: Olfactory dysfunction was frequent after nasal fracture (45/97, 46.4%). Our olfactory assessment using the KVSS II test revealed that fracture reduction was not associated with improvements in the mean test score in Type I or Type II fractures. More specifically, the mean posttraumatic Threshold, discrimination and identification score decreased from 28.8 points prior to operation to 23.1 point at 6 months for Type II fracture with septal fracture. Conclusion: Our study has revealed two alarming trends regarding post-nasal fracture olfactory dysfunction. First, our study demonstrated that almost half (46.4%) of nasal fracture patients experience posttraumatic olfactory dysfunction. Second, closed reduction of these fractures does not lead to improvements olfaction at 6 months, which suggest that olfactory dysfunction is probably due to factors other than the fracture itself. The association should be further explored between injuries that lead to nasal fracture and the mechanism behind posttraumatic olfactory dysfunction.

• 한국어류학회지
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• 제28권1호
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• pp.28-34
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• 2016

한국산 날개망둑 Favonigobius gymnauchen 후각기관은 2015년 7월과 8월 사이 전라북도 부안군 변산면 격포리의 조간대에서 채집된 개체들을 대상으로 실체현미경, 광학현미경 그리고 주사전자현미경을 이용하여 해부학 및 조직학적 특징들을 조사하였다. 후각기관은 두부 주둥이 위 좌우로 한 쌍이 존재하며, 전비공과 후비공, 한개의 비강, 두 개의 비낭, 후신경, 그리고 후구로 구성되었다. 비강 내 감각상피는 연결성 유형의 분포를 보였으며, 섬모성 감각세포만을 가지고 있었고, 감각세포, 지지세포, 기저세포들로 구성되었다. 비감각상피는 층상상피세포들로 구성되었으며, 표면에 많은 점액공을 가지고 있었다. 따라서 이러한 후각기관의 특징들은 연안지역 조수 웅덩이와 수심이 얕은 조간대의 생태적 서식처와 밀접한 관계가 있는 것으로 여겨진다.

• 한국융합학회논문지
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• 제7권2호
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• pp.61-67
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• 2016

후각기관은 주변 환경의 다양한 화학물질을 감지하는 기관으로 생존, 종족번식에서 감정에 이르기까지 다양하고 중요한 역할을 하고 있다. 유전적, 환경적 등 다양한 요소에 의해 후각장애가 발생할 수 있으며, 일시적인 경우에는 약물치료 등으로 회복될 수 있지만, 신경세포에 문제가 생긴 영구적인 손상의 경우는 치료가 어렵다. 따라서, 신경세포의 사멸을 억제하거나 재생을 유도하는 치료제의 개발이 필요하다. 본 연구에서는 후각신경세포 특이적으로 GFP 형광단백질을 발현하는 형질전환동물을 제작하여 생체 내 후각신경세포를 관찰하고자 하였다. 또한, 다양한 화학물질을 처리하여 후각신경세포 손상을 인위적으로 유도할 수 있는 방법을 고안하였고, 후각신경세포의 손상 및 재생 과정을 실시간으로 모니터링하였다. 본 연구를 통해 확립된 후각신경세포의 손상 및 재생 모니터링 시스템은 향후 후각신경세포 재생 메커니즘 연구 및 치료제 개발에 유용하게 사용될 것으로 기대된다.

• 대한두경부종양학회지
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• 제18권2호
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• pp.207-210
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• 2002

Schwannoma is a relatively rare benign tumor which may occur from nerve sheath of the peripheral, sympathetic and cranial nerves and so on except optic and olfactory nerves which have no nerve sheath themselves. Although it occur most frequently in the head and neck region especially in the acoustic nerve, the schwannoma originated from the motor nerves including hypoglossal nerve is very rare. Recently, we have experienced a case of schwannoma originated from hypoglossal nerve in a 47-year-old female. We report this case with literature review.

• 대한두경부종양학회지
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• 제25권2호
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• pp.171-173
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• 2009

Neurilemmoma is a relatively rare benign tumor which may be derived from nerve sheath of the peripheral, sympathetic and cranial nerve except the optic nerve and olfactory nerve. And it is a characteristically solitary and well-encapsulated mass. About 25% of neurilemmoma occurs in the head and neck region and the acoustic nerve is most frequently involved, but neurilemmoma of the larynx is rare. Recently, we experienced a case of neurilemmoma occurred in the right aryepiglottic fold of the larynx. The tumor was successfully removed by transoral approach under direct laryngoscope. So we report this case with literature review.

• Asian Spine Journal
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• 제12권6호
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• pp.998-1009
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• 2018

Study Design: Olfactory ensheathing cells (OECs) from rat olfactory mucosa were cultured, characterized, and transplanted into a rat model of spinal cord injury (SCI). Purpose: To evaluate different doses of OECs in a rat model of SCI. Overview of Literature: SCI causes permanent functional deficit because the central nervous system lacks the ability to perform spontaneous repair. Cell therapy strategies are being explored globally. The clinical use of human embryonic stem cell is hampered by ethical controversies. Alternatively, OECs are a promising cell source for neurotransplantation. This study aimed to evaluate the efficacy of different doses of allogenic OEC transplantation in a rat model of SCI. Methods: OECs were cultured from the olfactory mucosa of Albino Wistar rats; these cells were characterized using immunohistochemistry and flow cytometry. Rats were divided into five groups (n=6 rats each). In each group, different dosage ($2{\times}10^5$, $5{\times}10^5$, $10{\times}10^5$, and >$10{\times}10^5$) of cultured cells were transplanted into experimentally injured spinal cords of rat models. However, in the SCI group, only DMEM (Dulbecco's modified Eagle's medium) was injected. Rats were followed up upto 8 weeks post-transplantation. The outcome of transplantation was assessed using the Basso, Beattie, Bresnahan (BBB) scale; motor-evoked potential studies; and histological examination. Results: Cultured cells expressed 41% of p75NTR, a marker for OEC, and 35% of anti-fibronectin, a marker for olfactory nerve fibroblast. These cells also expressed $S100{\beta}$ and glial fibrillary acid protein of approximately 75% and 83%, respectively. All the transplanted groups showed promising BBB scores for hind-limb motor recovery compared with the SCI group (p<0.05). A motor-evoked potential study showed increased amplitude in all the treated groups compared with the SCI. Green fluorescent protein-labeled cells survived in the injured cord, suggesting their role in the transplantation-mediated repair. Transplantation of $5{\times}10^5$ cells showed the best motor outcomes among all the doses. Conclusions: OECs demonstrated a therapeutic effect in rat models with the potential for future clinical applications.