• 한국산업융합학회 논문집
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• 제23권3호
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• pp.381-392
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• 2020

The necessity and the importance of the remanufacturing are increasing day by day along with environmental problems. Many studies are being conducted on remanufacturing, but the research for cleaning is much lacking. This study aims to evaluate the effective cleaning method for remanufacturing of start motors, one of the automobile parts. The cleaning process consists of oil stain removal, drying and rust removal processes. In this study, the two processes were conducted except for the drying process which has little influence on cleaning. The methodology for cleaning agent selection, degreasing and rust removal process was presented. For each methodology, five analysis factors were calculated by two-way comparison according to the process, and the values were evaluated quantitatively by substituting them into the evaluation table. In the selection of cleaning agent, neutral system, ultrasonic cleaning in degreasing, and grinding in rust removal were selected as the best cleaning methods.

• Journal of Information Processing Systems
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• 제16권5호
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• pp.1008-1014
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• 2020

Aiming at the defect detection quality of denim fabric, this paper designs an improved algorithm based on the optimized Gabor filter. Firstly, we propose an improved defect detection algorithm of jean fabric based on the maximum two-dimensional image entropy and the loss evaluation function. Secondly, 24 Gabor filter banks with 4 scales and 6 directions are created and the optimal filter is selected from the filter banks by the one-dimensional image entropy algorithm and the two-dimensional image entropy algorithm respectively. Thirdly, these two optimized Gabor filters are compared to realize the common defect detection of denim fabric, such as normal texture, miss of weft, hole and oil stain. The results show that the improved algorithm has better detection effect on common defects of denim fabrics and the average detection rate is more than 91.25%.

• 기계저널
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• 제31권4호
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• pp.389-400
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• 1991

압정가공은 열간압정과 냉간압정으로 크게 나눌 수 있으며, 압정기와 피압정재의 종류에 따라 서도 분류한다. 열간압정에서는 알루미늄과 알루미늄합금을 제외하고는 종래에 오직 물만이 냉각겸 윤활제로 사용되어 왔다. 그러나 근년에 와서 열간압정에서도 윤활유를 사용하게 됨으 로서 롤(roll)의 수명연장, 전력소비량의 저감 및 사상(finishing) 강판의 표면품질향상등에 극히 유효하다는 것을 인식하게 되었다. 한편 냉각압정에서는 압정유를 사용해 온 것이 100년 가까이 되는 역사가 있고, 특히 과거 십 수년간에는 상당히 광범위하게 연구가 이루어져서, 많은 실적을 갖게 되었다. 예컨대, 석도원판으로 대표되는 박판압정에 있어서는 고속고압하에서 판의 형상 및 표면정쟁성 향상은 물론이고 저온(에너지 절감, 작업성 개선) 저농도사용(원단위감소)이 이루어 지고, 나아가 장수명(액의 오염이 적은 것)의 압정유가 개발되었다. 또한 마대강 압정에 있어 서는 고윤활이면서도 ECL(electric cleaning line)의 성략도 가능한 압정유 즉 밀크린시트(mill clean sheet) 압정유의 연구도 활발히 진행되고 있다. 압정유의 역할을 살펴보면 열간 또는 냉 간을 불문하고, 롤과 재료간의 감마작용이 주가 되지만 기타 실용적으로는 (1)냉각성 (2)판의 끝맺음(finishing)성 (3)선정선 (4)방장성,비부식성 (5) 내노화성 (6) 오일.스테인(oil stain) (7) 급 유성 (8)액관리성 (9)비수처리성 10) 안전위생 (11)경제성 등을 열거할 수 있다.

• Journal of Ginseng Research
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• 제47권4호
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• pp.572-582
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• 2023

Background: Free fatty acid-induced lipotoxicity is considered to play an important role in pancreatic β-cell dysfunction. The effect of ginsenosides on palmitic acid-induced pancreatic beta-cells cell death and failure of glucose-stimulated secretion of insulin (GSIS) was evaluated in this study. Methods: Enzyme-linked immunosorbent assay kit for a rat insulin was used to quantify glucose-stimulated insulin secretion. Protein expression was examined by western blotting analysis. Nuclear condensation was measured by staining with Hoechst 33342 stain. Apoptotic cell death was assessed by staining with Annexin V. Oil Red O staining was used to measure lipid accumulation. Results: We screened ginsenosides to prevent palmitic acid-induced cell death and impairment of GSIS in INS-1 pancreatic β-cells and identified protopanaxadiol (PPD) as a potential therapeutic agent. The protection effect of PPD was likely due to a reduction in apoptosis and lipid accumulation. PPD attenuated the palmitic acid-induced increase in the levels of B-cell lymphoma-2-associated X/B-cell lymphoma 2, poly (ADP-ribose) polymerase and cleaved caspase-3. Moreover, PPD prevented palmitic acid-induced impairment of insulin secretion, which was accompanied by an increase in the activation of phosphatidylinositol 3-kinase, peroxisome proliferator-activated receptor γ, insulin receptor substrate-2, serine-threonine kinase, and pancreatic and duodenal homeobox-1. Conclusion: Our results suggest that the protective effect of PPD on lipotoxicity and lipid accumulation induced by palmitic acid in pancreatic β-cells.

• 동의생리병리학회지
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• 제32권6호
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• pp.384-395
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• 2018

The aim of this study was to investigate whether a novel formulation of an herbal extracts has an inhibitory effect on obesity. To determine its anti-obesity effects, we performed anti-obesity-related experiments in vitro and in vivo. Thus, our present study was carried out to evaluate the anti-obesity effect of herbal extracts using a high fat diet (HFD)-induced obese mouse model and 3T3-L1 adipose cells. The effects of each herbal extracts on lipid accumulation in 3T3-L1 cells were examined using Oil Red O staining. Results showed that treatment with each herbal extracts at $10{\sim}100{\mu}g/ml$ had no effect on cell morphology and viability. Without evidence of toxicity, herbal extracts treatment decreased lipid accumulation compared with the untreated adipocytes controls as shown by the lower absorbance of Oil Red O stain. Futhermore, compared with control-differentiated mature adipocytes, each herbal extracts significantly inhibited lipid accumulation in mature 3T3-L1 adipocytes. In the HFD-fed obese mice, body weight, liver weight and white adipose tissue weights were significantly reduced by mixture of herbal extracts administration in mouse skin. Futhermore, we found that mixture of herbal extracts administration suppressed serum triglyceride (TG), and total cholesterol (TCHO) in HFD-induced obese mouse model. The mixture of herbal extracts of permeability was estimated by measuring the transepithelial electrical resistance (TEER) value in pig skin. The optimized formulations of herbal extracts (Test 3 formulation) showed skin permeation. However, test 1 formulation containing essential oil as enhancer showed maximum skin permeation. After confirming the enhanced skin permeability, in vivo studies were performed to assess whether skin irritation potential on the basis of a primary irritation index (PII) in rabbit skin. Reactions were scored for erythema/edema reactions at 24 h, 48 h and 72 h post-application. It was concluded that the test 1 formulation was not irritation (PII = 0). The present study suggests that the test 1 formulation might be of therapeutic interest with respect to the treatment of obesity.

• KIEAE Journal
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• 제12권5호
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• pp.71-76
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• 2012

As reviewed of developments of Korean earth architecture, earth-made buildings have been dwindled gradually since "the new community movement" and earth have been recognized as materials which are not so good to human health and in result, earth-related techniques have been also forgotten. However, recently the earth architecture has been more attracting back many attentions thanks to the people who are interested in health and wellbeing and the earth related techniques or skills got keenly required. The present study has investigated and reproduced earth finishing materials which are based on natural materials as basic stuffs to use them as the finishing materials of the modern earth architecture. For the test, the finishing materials have been divided roughly into sorts of pastes and oils. In case of applying finishing materials onto earth surfaces, the moisture permeability test was conducted to measure a water-absorbing speed, and at the case of using finishing materials for interior works moisture adsorption/desorption test was performed to verify the indoor humidity regulation ability, and further a test to check whether to be stained when contacted with the finishing materials, was conducted. If there is not any stain it might be recognized to be high quality of moisture adsorption/desorption and so seaweed pastes or starches might be used for paste finishing materials and for natural oils, beans are desirable in practical ways. As low-quantity of moisture adsorption materials, linseed oil is most desirable and also beaned water over75% are thought to be useful.

• Nutrition Research and Practice
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• 제8권6호
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• pp.613-617
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• 2014

BACKGROUND/OBJECTIVES: Adipogenesis is part of the cell differentiation process in which undifferentiated fibroblasts (pre-adipocytes) become mature adipocytes with the accumulation of lipid droplets and subsequent cell morphological changes. Several transcription factors and food components have been suggested to be involved in adipogenesis. The aim of this study was to determine whether mulberry leaf ethanol extract (MLEE) affects adipogenesis in 3T3-L1 adipocytes. MATERIALS/METHODS: The 3T3-L1 adipocytes were treated with different doses of MLEE for 8 days starting 2 days post-confluence. Cell viability, fat accumulation, and adipogenesis-related factors including CCAAT-enhancer-binding protein alpha ($C/EBP{\alpha}$), peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$), $PPAR{\gamma}$ coactivator 1 alpha (PGC-$1{\alpha}$), fatty acid synthase (FAS), and adiponectin were analyzed. RESULTS: Results showed that MLEE treatments at 10, 25, 50, and $100{\mu}g/ml$ had no effect on cell morphology and viability. Without evident toxicity, all MLEE treated cells had lower fat accumulation compared with control as shown by lower absorbances of Oil Red O stain. MLEE at 50 and $100{\mu}g/ml$ significantly reduced protein levels of $PPAR{\gamma}$, PGC-$1{\alpha}$, FAS, and adiponectin in differentiated adipocytes. Furthermore, protein level of $C/EBP{\alpha}$ was significantly decreased by the treatment of $100{\mu}g/ml$ MLEE. CONCLUSION: These results demonstrate that MLEE treatment has an anti-adipogenic effect in differentiated adipocytes without toxicity, suggesting its potential as an anti-obesity therapeutic.

• Nutrition Research and Practice
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• 제9권6호
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• pp.606-612
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• 2015

BACKGROUND/OBJECTIVES: Several medicinal properties of Smilax china L. have been studied including antioxidant, anti-inflammatory, and anti-cancer effects. However, the antiobesity activity and mechanism by which the water-soluble fraction of this plant mediates its effects are not clear. In the present study, we investigated the lipolytic actions of the water-soluble fraction of Smilax china L. leaf ethanol extract (wsSCLE) in 3T3-L1 adipocytes. MATERIALS/METHODS: The wsSCLE was identified by measuring the total polyphenol and flavonoid content. The wsSCLE was evaluated for its effects on cell viability, lipid accumulation, glycerol, and cyclic adenosine monophosphate (cAMP) contents. In addition, western blot analysis was used to evaluate the effects on protein kinase A (PKA), PKA substrates (PKAs), and hormone-sensitive lipase (HSL). For the lipid accumulation assay, 3T3-L1 adipocytes were treated with different doses of wsSCLE for 9 days starting 2 days post-confluence. In other cell experiments, mature 3T3-L1 adipocytes were treated for 24 h with wsSCLE. RESULTS: Results showed that treatment with wsSCLE at 0.05, 0.1, and 0.25 mg/mL had no effect on cell morphology and viability. Without evidence of toxicity, wsSCLE treatment decreased lipid accumulation compared with the untreated adipocyte controls as shown by the lower absorbance of Oil Red O stain. The wsSCLE significantly induced glycerol release and cAMP production in mature 3T3-L1 cells. Furthermore, protein levels of phosphorylated PKA, PKAs, and HSL significantly increased following wsSCLE treatment. CONCLUSION: These results demonstrate that the potential antiobesity activity of wsSCLE is at least in part due to the stimulation of cAMP-PKA-HSL signaling. In addition, the wsSCLE-stimulated lipolysis induced by the signaling is mediated via activation of the ${\beta}$-adrenergic receptor.

• Journal of the Korean Wood Science and Technology
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• 제33권4호통권132호
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• pp.15-22
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• 2005

현악기용 도료가 현악기의 음향성능에 미치는 영향을 조사하기 위해 음향분석장치(FFT)와 점탄성 분석장치(DMTA, dynamic mechanic thermal analyzer)를 이용하여 목재악기용 도료의 음향적 성능을 평가하였다. 목재악기용으로 사용된 도료는 악기용 우레탄계 상도도료, 오일 스테인계 도료, 천연바니쉬, 캐슈 도료 및 자외선 경화형 에폭시 도료를 사용하였다. 형성된 도막의 음향적인 특성은 FFT 분석기에서 측정된 공명주파수와 댐핑값(damping factor)과 DMTA에서 측정된 tan ${\delta}$를 통하여 유추하였다. FFT에서 측정된 공명주파수와 댐핑값으로부터 도막이 가지는 음향특성에 대해 분석하였다. 측정된 도막의 점탄성적인 특성과 음향적 특성을 검토한 결과 악기용으로서의 적정도료를 결정하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권3호
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• pp.432-439
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• 2007

Thiazolidinediones (TZDs) act as potent activators of the adipose differentiation program in established preadipose cell lines. TZD's have also been investigated in diabetic patients and reported to act as PPAR-${\gamma}$ ligands. In this report, the effects of TZDs on the differentiation pathway of myoblasts have been investigated. C2C12 mouse myoblasts were grown in Dulbecco's Modified Eagles medium for 4-5 days until they reached almost 100% confluency. Post-confluent cells (day 0) were further exposed to adipogenic induction medium along with TZDs for 48 hours. Thereafter, cells were exposed only to TZDs every 48 h until day 10. The control was provided with differentiation medium without any treatment. Alterations in the cells during the differentiation programme were analyzed on the basis of fusion index, oil-red-o staining, adipocyte index, adipocyte stain uptake measurement, immuno-histochemistry and western blotting. Exposure of C2C12 mouse myoblasts to TZDs prevented the expression of myosin heavy chain with parallel increase in the expression of C/EBP-${\alpha}$ and PPAR-${\gamma}$ and acquisition of adipocyte morphology, thus abolishing the formation of multinucleated myotubes. TZDs exert their adipogenic effects only in non-terminally differentiated myoblasts; myotubes were insensitive to the compound. Continuous exposure (at least 4-5 doses) to inducers after the growth arrest was essential to provide a sustained environment to the cells converting to fully matured adipoctyes. The results indicate that TZDs specifically converted the differentiation pathway of myoblasts into that of adipoblasts.