• Mycobiology
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• 제48권6호
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• pp.484-494
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• 2020

Oak wilt disease caused by Raffaelea quercus-mongolicae has emerged obviously in Korea. We selected antifungal isolates against R. quercus-mongolicae among 368 endophytic fungal isolates from different parts of oak and pine trees. The experiment was conducted in the primary and secondary screenings by dual culture test. The antifungal activity of the selected isolates was assessed in culture filtrate test based on the inhibition rates in mycelial growth, sporulation, and spore germination of oak wilt fungus. Five isolates, E089, E199, E282, E409 and E415, showed strong antifungal activity in culture filtrate test, and their antifungal activity decreased on the culture media supplemented with heated culture filtrate. Higher mycelial growth inhibitions on the unheated media were recorded in E409 (Colletotrichum acutatum), E089 (Daldinia childiae), E415 (Alternaria alternata) and E199 (Daldinia childiae) with the inhibition rates of 79.0%, 70.1%, 68.9% and 64.5%, respectively. These isolates also had the higher sporulation inhibitions on unheated media with the rates of 96.8%, 84.2%, 82.8% and 80.5%, respectively. The spore germination of the oak wilt fungus was completely inhibited by E282 (Nectria balsamea) on both unheated and heated media. These results showed that a higher number of potent antifungal isolates against oak wilt fungus was isolated from the petiole compared to the other parts. This study could contribute to the development of biological control approaches for the management of oak wilt disease caused by R. quercus-mongolicae.

• Journal of Korean Society of Forest Science
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• 제103권4호
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• pp.639-645
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• 2014

This study was conducted to evaluate the management efficiency of oak mushroom farms in Korea using the Data Envelopment Analysis (DEA), which is one of the non-parametric estimation methods. The data that was analyzed in this study was from the result of 2013 survey entitled "Standard Diagnostic Table for Oak Mushroom Management", which was conducted from March 2012 to October 2012. This survey was based on the inputs and outputs of 20 oak mushroom farms. Specifically, this study analyzed the technical efficiency, pure-technical efficiency and scale efficiency using CCR and BCC model of the DEA methods. Furthermore, this study compares the management efficiency between the full time oak mushroom production farms and part time oak mushroom production farms. Results showed that mean value for the technical efficiency was 0.655 which is considered as inefficient in general. For the pure-technical efficiency and scale efficiency, the mean values were 0.830 and 0.747, respectively which showed that inefficiency in the management was observed in the mushroom farms. Results also showed that there were seven farms with a total efficiency of 1, namely Decision Making Unit(DMU)2, DMU5, DMU6, DMU8, DMU10, DMU15 and DMU20. The management efficiency of DMU7 specifically the inputs for production was analyzed and compared to DMU5 and DMU6 and results showed that the DMU7 had an excessive inoculation and site development cost. Lastly, it was also observed that the full time mushroom production farms were more efficient as compared to the part time mushroom farms because of the lower scale efficiency value or smaller area for mushroom production allotted in the part time farms.

• Journal of Forest and Environmental Science
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• 제37권4호
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• pp.269-279
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• 2021

This study was carried out to introduce the forest cover types of the Baekdudaegan inhabiting the number of native tree species. In order to understand the vegetation distribution characteristics of the Baekdudaegan, a vegetation survey was conducted on the major 20 mountains of the Baekdudaegan. The vegetation data were collected from 3,959 sample points by the point-centered quarter method. Each mountain was classified into 4-7 forests by using various multivariate statistical methods such as cluster analysis, indicator species analysis, multiple discriminant analysis, and species composition analysis. The forests were classified mainly according to the relative abundance of Quercus mongolica. There was a total of 111 classified forests and these forests were integrated into the following nine forest cover types using the percentage similarity index and by clustering according to vegetation type: 1) Mongolian oak, 2) Mongolian oak and other deciduous, 3) Oaks (Mixed Quercus spp.), 4) Korean red pine, 5) Korean red pine and oaks, 6) ash, 7) mixed mesophytic, 8) subalpine zone coniferous, and 9) miscellaneous forest. Forests grouped within the subalpine zone coniferous and miscellaneous classifications were characterized by similar environmental conditions and those forests that did not fit in any other category, respectively.

• Journal of Korean Society of Forest Science
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• 제98권6호
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• pp.772-779
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• 2009

This study was conducted to estimate the carbon (C) contents in pure and mixed stands of pine (Pinus densiflora) and oak (Quercus spp.) trees for establishing the C inventory of forest ecosystems. A total of fifteen 20 m${\times}$20 m pure and mixed stands of pine and oak trees were chosen in natural forests in Hoengseong, Kangwon based on the basal area of all trees ${\geq}$ 5 cm DBH: three of 95% of pine and 5% oak trees [pine stand], three of 100% of oak trees [oak stand], and nine of 20 to 70% of pine and 80 to 30% of oak trees [mixed stand]. To estimate C contents in the study stands, biomass in vegetation, forest floor and coarse woody debris (CWD) were calculated and C concentrations in vegetation, forest floor, CWD and soil (0-30 cm) were analyzed. There was no significant difference in vegetation C contents among the stands; 147.6 Mg C/ha for the oak stand, 141.4 Mg C/ha for the pine stand and 115.8 Mg C/ha for the mixed stand. Forest floor C contents were significantly different among the stands (p<0.05); 12.7 Mg/ha for the pine stand, 9.9 Mg/ha for the oak stand, and 8.4 Mg/ha for the mixed stand. However, CWD C contents were not significantly different among the stands (p>0.05); 2.2 Mg/ha for the mixed stand, 1.7 Mg/ha for the oak stand, and 1.1 Mg/ha for the pine stand. Soil C contents up to 30 cm depth were not significantly different among the study stands; 44.4 Mg C/ha for the pine stand, 41.6 Mg C/ha for the mixed stand, and 33.3 Mg C/ha for the oak stand. Total ecosystem C contents were lower in the mixed stand than those in the pure stands, because vegetation C contents which occupied almost total ecosystem C contents were lower in the mixed stand than those in the pure stands; 199.6 Mg C/ha for the pine stand, 192.5 Mg C/ha for the oak stand and 169.1 Mg C/ha for the mixed stand. Lower vegetation C contents in the mixed stand might be influenced by interspecific competition between pine and oak trees and intraspecific competition among the oak trees resulted from high stand density. We suggest that forest management such as thinning to enhance C storage is indispensible for minimizing the competition in forest ecosystems.

• Korean journal of applied entomology
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• 제47권2호
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• pp.127-131
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• 2008

Ambrosia beetle, Platypus koryoensis, is a vector of oak wilt disease caused by Raffaelea sp. in Korea. The degree of damage by oak wilt disease was dependent on the density of the beetles in the oak trunk, a relationship between the degree of damage by oak wilt disease and the density of beetle on the basis of the number of entry hole was studied. Entry hole distribution within tree trunk was analyzed by the nearest neighbor method. Thirty four oak trees (Quercus mongolica) located in survey area were selected and then degree of damage, the number of attack hole/$623cm^2$ in upper (50cm from the surface) and lower (surface) trunk and the nearest neighbor distance between the holes were measured. The number of hole and the nearest neighbor distance in upper and lower part were positively correlated with each other. As the degree of damage was severer, the number of the holes increased, whereas the nearest neighbor distance decreased. The distribution pattern of the hole was changed from clumped one to uniform as the severity of damage increased. These results suggested that Platypus koryoensis attacked the oak tree in concentrative manner at initial stage of attack but at final stage, it distributed uniformly to reduce intraspecific competition between the beetles.

• Journal of Mushroom
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• 제14권3호
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• pp.119-126
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• 2016

Five empirical farmhouses were selected to reduce the high temperature damage in oak mushroom cultivation using bed-logs. The cultivation facilities were categorized as follows: those having two blackout curtains or one blackout curtain and outdoor oak mushroom cultivation. The inequality of the indoor condition, oak mushroom hyphae rampant ratio, and fruit body production in each test condition was evaluated. $3^{\circ}C$ was lower in indoor temperature of cultivation facility having two blackout curtains than one blackout curtain. Specifically, the indoor air humidity average of cultivation facilities having one or two blackout curtains was 10% lower than that of outdoor oak mushroom cultivation. This condition is not ideal for oak mushroom cultivation as continuous indoor humidity control is essential for producing good fruit bodies. The Inoculated bed-log surface and oak mushroom hyphae rampant ratio of bed-logs cultivated with two blackout curtains was superior to other tested conditions. The mushroom production ratio observed in facilities with two blackout curtains was 117-204% higher than those cultivated in facilities with only one blackout curtain. Furthermore, the mushroom production ratio increased in based on these findings, we recommended five cultivation facility models to reduce high temperature damage in oak mushroom cultivation using bed-logs.

• The Korean Journal of Ecology
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• 제17권3호
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• pp.319-331
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• 1994

A phutosociological study on the Mongolian oak forest was carried out on Mt. Sorak by the Z-M method. The Mongolian oak forest was classified into one oder, two alliances, one assoication and three commuities; Rhododendro-Quercetalia mongolicae Kim 1990 Lindero-Quercion monogolicae Kim 1990 Vaccinium hirtum v. koreanum-Quercus monogolica community Lespedeza maximowiczii-Quercus monogolica community Pino koreiensis-Quercion mongolicae Kim 1992 Typical community Lichno-Quercetrum monofolicae Kim 1992 Total vascular plant species in invesitigatied sites consited of 193 taxa, and their life-form composition was $H-D_1-R_5-e$ type.

• Research in Plant Disease
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• 제20권4호
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• pp.295-298
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• 2014

Oak wilt caused by Raffaelea quercus-mongolicae was first noticed in South Korea in 2004 and, ever since, its distribution and damage have been increasing. To screen a fungicide effective for oak wilt control by tree injection, laboratory and field experiments were conducted. Ten fungicides and one antibiotic were examined in vivo for their effectiveness in restricting the growth of R. quercus-mongolicae and R. quercivora (Japanese oak wilt pathogen) isolates. To the Korean isolates of R. quercus-mongolicae, chlorothalonil showed the highest fungicidal effects, followed by benomyl and propiconazole. To the Japanese one, propiconazole was highest in the fungicidal effectiveness, followed by benomyl and bitertanol. Propiconazole was selected for field-testing of its control efficacy because it showed good fungicidal effects in vitro and systemic activity. The control efficacy in the field was 87.5% in the first year of injection and 66.7% in the second year, indicating the fungicidal effects last at least over one year.

• Journal of Forest and Environmental Science
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• 제33권4호
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• pp.342-347
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• 2017

Since the first report of the oak wilt disease at 2004 in Korea, the disease distributed over Korean peninsula and are still giving severe damages. The management of oak wilt disease in Korea has mainly focused on the control of insect vector, Platypus koryoensis. Neverthless the effective method for evaluating the pathogenicity of the pathogen, Raffaelea quercus-mongolicae (Rqm), and for screening chemical or biological agents with strong inhibitory activity against the pathogen, is absolutely necessary, an reliable method is not available so far. This study was conducted to develop the effective method for evaluating the pathogenicity of Rqm in oak trees. The culture suspensions of Rqm were artificially injected to the saplings of Quercus acutissima by using ChemJet tree injector. Three months after treatments, the treated saplings were cut and dipped into 1% fuchsin acid solution. There were significant differences in non-conductive area (%), discoloration area (%) and vertical discoloration length between the pathogen-injected and distilled water-injected control treatments. These results indicated that the pathogen is the causal agent for the dysfunction of water conductive tissue, which will finally result in wilt symptom. Re-isolation of the pathogen and PCR detection using specific primers for the pathogen also confirmed the presence of Rqm in the sapwood chips of the pathogen-injected saplings. These observations would be greatly applied to other related researches for evaluating the pathogenicity of tree wilt pathogens and biocontrol efficacy of the selected antagonistic microorganisms, in case that the wilt symptom is not easily shown by artificial inoculation of the causal agent.

• The Plant Pathology Journal
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• 제18권2호
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• pp.74-76
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• 2002

This study unexpectedly detected a deletion in the promoter region of ras gene in two Korean strains of oak mushrooms, Lentinula edodes (Berk.). Sequencing of the promoter regions revealed that one type consisting of two strains had a 113 bp deletion in the region. The pas promoter region of Korean strains differed by 16 bases from that of the Japanese strains. Between the two types of Korean strains, except for the deleted portion, only a single site appeared to be different.