• 한국수정란이식학회지
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• 제21권3호
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• pp.255-262
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• 2006

본 연구는 Open Pulled Straw(OPS)방법에 의해 동결-융해한 돼지 수정란의 체외 생존 능력을 검토하기 위하여 수행되었다. 미성숙 난자의 체외 성숙을 위하여, 돼지 난소는 도축장에서 회수하였으며, 난구세포로 쌓여있는 난자는 직경 $2{\sim}6mm$의 난포로부터 난포액과 함께 흡입에 의하여 회수하였다. 회수된 미성숙 난자의 체외 성숙을 위하여 5 mM hypotaurine, 0.57 mM cysteine, 10% 난포액, 10 IU/ml PMSG 및 10 IU/ml hCG가 함유된 NCSU-23 배양액 내에서 $21{\sim}22$ 시간 배양 후, 호르몬 물질을 제거한 성숙 배양액 내에서 $21{\sim}22$ 시간 동안 추가 배양하였다. 한편 체외 수정을 위하여 동결-융해한 정액은 5.56 mM glucose, 0.33 mM na-pyruvate, 100 IU/ml penicillin, $100 {\mu}g/ml$ streptomycin 및 4 mg/ml BSA가 첨가된 D-PBS 액을 가지고 1,500 rpm에서 10분간 2회 원심분리를 실시하여 세척하였다. 체외 수정을 위한 배양액은 pH $7.2{\sim}7.4$에서 2 mM caffeine과 2 mg/ml BSA가 첨가된 mTBM 배양액을 이용하였다. 체외 수정시 정자의 최종 농도는 $2.5{\times}10^6cells/ml$로 조정하였다. 수정 8시간 후, 체외 발육을 위하여 5.0 mM hypo-taurine, 4 mg/ml BSA 및 10 ng/ml EGF가 첨가된 NCSU- 23액으로 옮겨 7일간 배양을 계속하였다. 그 후 배반포의 여러 단계에서 OPS 법에 의해 동결-보존하였다. 그 결과, 동결-융해 후 형태학적으로 정상적인 수정란의 비율은 초기 배반포(28.3%)보다는 확장 배반포(38.9%)단계에서 동결했을 때 유의적으로 높게 나타났다(p<0.05). 한편, 동결-융해 후 상해를 입은 수정란의 비율은 확장 배반포보다는 초기 배반포 단계에서 동결된 수정란에서 유의적으로 높은 성적을 보였다(p<0.05). 또 다른 실험에서, 수정란의 동결-융해 후 형태학적으로 정상인 수정란을 48시간 추가 배양했을 때, Hatching 된 수정란의 비율은 초기 배반포, 배반포 및 확장배반포기 단계에서 동결-융해한 수정란에서 각각 6.7, 20.0 및 33.3%로 발육 단계가 높을수록 동결-융해 후의 생존율도 높게 나타났다. 본 연구의 결과로부터, 돼지에서 수정란의 동결-융해 후 생존성의 향상을 위해서는 발육 단계가 높은 배반포기 단계에서의 동결이 요구되며 본 연구에서 이용된 OPS 동결 방법이 폭넓게 활용될 것으로 사료된다.

• 정보처리학회논문지D
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• 제13D권2호
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• pp.207-214
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• 2006

서열화가 완성된 유전체의 수가 최근에 빠르게 상승하고 있지만, 상동성에 의한 단백질 기능을 예측하는 방법은 충분히 연구되고 있지 않다. 서열화가 완성된 다수의 유전체로부터 유전체 상호간의 BLAST 최대 히트(best-hit)를 사용하여 OPCs(Orthologous Protein Clusters)를 만드는 일은 성공적으로 연구되어 왔다. 그러나 OPCs를 수작업으로 구축하는 것은 시간과 노력이 많이 드는 일이다. 이 논문에서 우리는 서열화가 완성된 다수의 유전체로부터 OPs(Orthologous Proteins)를 클러스터링하는 자동화 방법을 제시하고, 해당 클러스터링의 타당성을 수학적으로 증명 한다.

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2006년도 The 6th Intermational Symposium on Developmental Biotechnology
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• pp.40-41
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• 2006

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2002년도 국제심포지엄
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• pp.84-84
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• 2002

The advantages of the OPS techniques(Vajta G et al, Mol Reprod Dev 51: 53-58,1998) give 1) high survival rates of various types of eggs, 2) quick and simple process, 3) inexpensive equipment and reduced chilling injury. The efficiency of IVM/IVF technique in the porcine species is relatively lower than that obtained in other species such as ruminants. Two experiments were designed to investigate the effects of in-vitro fertilization of porcine oocytes matures using different OPS protocol for chilling and warming of vitrification. Porcine oocytes from ovaries collected at abattoir were matured for 44 hours in TCM199 Earle's salt supplemental with pyruvate, pff, L-cysteine, hormones and gentamycin. Oocytes were denuded and fertilized with frozen boar semen by common method. Porcine embryos produced routinely by in-vitro culture system of NCSU23 medium. The vitrification and the warming were conducted by OPS method with the glass micropipette instead of straw vessels and modified the protocol of G.Vajta(1999). In Exp 1, Chilling/Warming:Holding Medium(HM)+EG+DMSO/HM +sucrose Medium(SM) at 39$^{\circ}C$ warm stage. In Exp 2, : PBS+CS+EG+Ficoll+ Trehalose/PBS+Trehalose at 25$^{\circ}C$ stage. Filling, freezing, packing, thawing out and further culturing were performed to follow the basic protocol of G Vajta. During IVM-lVC and post-warming, fertilization parameter and developmental potential were compared to and statistically analysed. It was not significantly different from Exp 1 and Exp 2 but 25$^{\circ}C$ of stage was slightly higher on the morula/blastocyst forming rate and better atmosphere for worker than that at 39$^{\circ}C$ stage.

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2003년도 제3회 발생공학 국제심포지움 및 학술대회
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• pp.108-108
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• 2003

• 조명전기설비학회논문지
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• 제22권1호
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• pp.66-72
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• 2008

최근 한약재의 저장 중 품질 유지를 위한 다양한 방안이 검토되고 있으며, 그 방안의 하나로 한약재의 유해 세균 및 잔존 농약을 분해, 처리하여 저장하는 방법을 연구하였다. 오존발생기에 관한 선행 연구를 통해 오존이 박테리아 및 비루스에 대한 살균효과가 우수함을 확인하고, 오존을 이용하여 한약재 살균처리 및 저장(이하 OPS)에 적용하는 방법을 연구하였다 본 연구에서는 현재 시중에 많이 유통되어 사용되고 있는 4종류의 약재를 선정하여 한약재 특성 평가를 실시하였다. 즉 오존 램프를 이용하여 한약재 살균 특성을 조사하였고, 장기간 오존 저장 후 한약재의 품질 및 고유 성분이 유지되는지를 조사하기 위하여 한약재의 일반성분 및 생리활성물질의 정량분석 평가를 하였다.

• 한국멀티미디어학회논문지
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• 제23권2호
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• pp.328-342
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• 2020

As the cloud-based ICT(Information & Communication Technology) infrastructure matures, the existing monolithic software development method is evolving into a micro-service structure based on cloud native computing. To develop and operate the services efficiently under the cloud native environment, DevOps-based application development plans through MSA(Micro Service Architecture) design based are essential. A cloud native environment is an approach to developing and running applications that take advantage of cloud computing models such as automation of source distribution, container-based virtualization, application scalability, resource efficiency, and flexible maintenance through object independence. To implement this approach, the utilization of key elements such as DevOps, continuous delivery, micro service, and containers is essential, but there are not enough previous studies on case analyses or application methods of these key elements. Therefore, in this paper, we analyze the cases of application development in cloud native environment and propose the optimized application development process and development method through small and medium-sized SI projects.

• Steel and Composite Structures
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• 제49권6호
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• pp.645-666
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• 2023

Due to the steadily declining supply of natural coarse aggregates, the concrete industry has shifted to substituting coarse aggregates generated from byproducts and industrial waste. Oil palm shell is a substantial waste product created during the production of palm oil (OPS). When considering the usage of OPSC, building engineers must consider its uniaxial compressive strength (UCS). Obtaining UCS is expensive and time-consuming, machine learning may help. This research established five innovative hybrid AI algorithms to predict UCS. Aquila optimizer (AO) is used with methods to discover optimum model parameters. Considered models are artificial neural network (AO - ANN), adaptive neuro-fuzzy inference system (AO - ANFIS), support vector regression (AO - SVR), random forest (AO - RF), and extreme gradient boosting (AO - XGB). To achieve this goal, a dataset of OPS-produced concrete specimens was compiled. The outputs depict that all five developed models have justifiable accuracy in UCS estimation process, showing the remarkable correlation between measured and estimated UCS and models' usefulness. All in all, findings depict that the proposed AO - XGB model performed more suitable than others in predicting UCS of OPSC (with R², RMSE, MAE, VAF and A_15-index at 0.9678, 1.4595, 1.1527, 97.6469, and 0.9077). The proposed model could be utilized in construction engineering to ensure enough mechanical workability of lightweight concrete and permit its safe usage for construction aims.

• Clinical and Experimental Reproductive Medicine
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• 제30권3호
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• pp.241-248
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• 2003

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

• 전자공학회논문지B
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• 제32B권3호
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• pp.89-99
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• 1995

One of the hardest problems that limit real application of production system is its slowness. One way to overcome this problem is to speed up the matching operation which occupies more than 90% of the total execution time. In this paper, we try to speed up the matching operation with parallel execution of a typical pattern matching algorithm, RETE, in a multiprocessor environment, This requires not only to make partitions of the rules but also to allocate the partitioned rules to processors, respectively. A partition strategy is proposed to make groups of similar rules by evaluating the similarity of rules according to the number of common conditions between rules. An allocation strategy is proposed to make the load of each processor even by assigning the different priority to the group of rules according to the expected amount of time required for matching operation. To compare with the existing methods, we perform simulation using OPS5 sample programs. The simulation results show that the proposed methods can improve the performance of production system.