• 한국해양공학회지
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• 제32권5호
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• pp.297-309
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• 2018

After an accident involving mooring link failures in an offloading buoy, verification of the fatigue safety in terms of the out-of-plane bending (OPB) and in-plane bending (IPB) moments has become a key engineering item in the design of various floating offshore units. The mooring links for an 8 MW floating offshore wind turbine were selected for this study. To identify the OPB stiffness (OPB moment versus interlink angle), a numerical simulation model, called the 3-link model, is usually composed of three successive chain links closest to the fairlead or chain hawse. This paper introduces two numerical simulation techniques for the 3-link analyses. The conventional and advanced approaches are both based on the prescribed rotation approach (PRA) and direct tension approach (DTA). Comparisons of the nominal stress distributions, OPB stiffnesses, hotspot stress curves, and stress concentration curves are presented. The multiple link analyses used to identify the tension angle versus interlink angle require the OPB stiffness data from the 3-link analyses. A convergence study was conducted to determine the minimum number of links for a multi-link analysis. It was proven that 10 links were sufficient for the multi-link analysis. The tension angle versus interlink angle relations are presented based on multi-link analyses with 10 links. It was found that the subsequent results varied significantly according to the 3-link analysis techniques.

• International Journal of Industrial Entomology and Biomaterials
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• 제7권1호
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• pp.11-14
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• 2003

NF733xin, the near allele line was obtained by means of crossing and backcrossing the silkworm race T6, which contained fluoride resistance major gene, to race 733xin, which was highly susceptible to fluoride toxicity. Two hundred RAPD random primers were used in the RAPD analysis of these 3 strains. Two molecular markers, OPB-08850 and OPB-10917, were obtained. OPB-10917 was used to detect the backcross generations. It was found that all the fluoride resistant individuals in each backcross generation had the same special band. These results proved that this marker was reliable.

• 생명과학회지
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• 제32권8호
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• pp.595-600
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• 2022

Oenanthe javanica와 O. javanica var. japonica 한국 내 식용미나리이다. Cicuta virosa는 독미나리로 시큐톡신을 함유하고 있다. 미나리에 대한 RAPD 마크에 의한 분자적 변이를 조사하였다. RAPD 분석을 위해 10개 올리고프라이머(오페론, OPB)로 6개 집단을 분석하였다. 6개 집단에서 72개 DNA 분절(밴드)을 찾았다. 72 밴드 중 61개(84.7%) 밴드는 다형현상을 나타내었다. 한국 내 독미나리 자연 집단은 작고 격리되어 패치 분포를 이루고 있지만 높은 유전적 다양도를 가지고 있었다. 반면에, 재배종 미나리(Oenanthe javanica var. japonica) 집단은 채소용으로 논에서 광범위한 분포를 나타내지만 독미나리와 야생종 미나리에 비해 낮은 유전적 다양도를 나타내었다. 비록 야생집단이 재배집단에 비해 다양도가 높지만 유의한 차이는 없었다. 전체 유전적 다양도(H_T)는 0.342였다. 집단 내 유전적 다양도(H_S)는 0.201이였다. 유전자 좌위에 근거한 집단 간 분화에서 전체 유전적 다양도의 비율(G_ST)은 0.414이므로 전체 변이의 41.4%는 집단 간에 있었다. 결론으로 RAPD기법은 독미나리와 식용미나리의 동정에 유용하였다. 또, RAPD의 OPB 마크는 미나리의 다른 자원과 식품 자원을 식별하는 데 도움이 될 수 있는 분자 마크임을 보여주었다.

• Ocean Systems Engineering
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• 제14권2호
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• pp.171-210
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• 2024

This paper focuses on the rigorous and holistic fatigue analysis of mooring chains for a deep draft semi-submersible platform in the challenging environment of the central Gulf of Mexico (GoM). Known for severe hurricanes and strong loop/eddy currents, this region significantly impacts offshore structures and their mooring systems, necessitating robust designs capable of withstanding extreme wind, wave and current conditions. Wave scatter and current bin diagrams are utilized to assess the probabilistic distribution of waves and currents, crucial for calculating mooring chain fatigue. The study evaluates the effects of Vortex Induced Motion (VIM), Out-of-Plane-Bending (OPB), and In-Plane-Bending (IPB) on mooring fatigue, alongside extreme single events such as 100-year hurricanes and loop/eddy currents including ramp-up and ramp-down phases, to ensure resilient mooring design. A detailed case study of a deep draft semi-submersible platform with 16 semi-taut moorings in 2,500 meters of water depth in the central GoM provides insights into the relative contributions of wave scatter diagram, VIMs from current bin diagram, the combined stresses of OPB/IPB/TT and extreme single events. By comparing these factors, the study aims to enhance understanding and optimize mooring system design for safety, reliability, and cost-effectiveness in offshore operations within the central GoM. The paper addresses a research gap by proposing a holistic approach that integrates findings from various contributions to advance current practices in mooring design. It presents a comprehensive framework for fatigue analysis and design optimization of mooring systems in the central GoM, emphasizing the critical importance of considering environmental conditions, OPB/IPB moments, and extreme single events to ensure the safety and reliability of mooring systems for offshore platforms.

• 한국발생생물학회지:발생과생식
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• 제10권1호
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• pp.19-32
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• 2006

참게(Eriocheir sinensis)와 꽃게(Portunus trituberculatus)의 2종으로부터 genomic DNA를 분리 추출하였다. 선택된 7개의 OPA-05, OPA-13, OPA-16, OPB-06, OPB-15, OPB-17 and OPD-10의 RAPD primer를 이용하여 identical, polymorphic 그리고 specific fragment를 얻어냈다. 본 연구에서 부안산 참게 집단에서는 505개의 fragment가 나타났고, 꽃게 집단에서는 513개의 fragment가 확인되었다. 참게 집단에서는 165개의 identical fragment가 나타났으며, 이는 primer당 평균적으로 23.6개의 fragment로 확인되었다. 또한 꽃게 집단에서는 66개로서 평균해서 primer당 9.4개의 identical fragment가 나타났다. 참게 집단과 꽃게 집단의 polymorphic fragment는 각각 50개와 14개로 나타났고, 참게 집단과 꽃게 집단의 경우 OPB-17에서 identical fragment가 300 bp의 크기에서 확인되었다. 각각을 비교해 보았을 때 유전적 차이는 참게 집단에서보다 꽃게 집단에서 더 높은 수치를 나타내었고, 2종 사이에서 $0.726{\pm}0.004$의 수치를 나타내었다. 7개의 primer를 사용하여 얻어진 dendrogram은 cluster 1(FRESHWATER 01), cluster 2(FRESHWATER 02, 03, 04, 05 및 06), cluster 3(FRESHWATER 07, 08, 09, 10 및 11) 및 cluster 4(SWIMMING 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 and 22)와 같이 4개의 유전적 클러스터로 나뉘어졌다. 꽃게 집단에서 18번째 개체(SWIMMING no. 18)와 17번째 개체 (SWIMMING no. 17) 사이가 가장 가까운 유전적 관계(genetic distance 0.096)를 나타내었다. 궁극적으로 볼 때 참게 집단의 2번째(FRESHWATER no. 02)와 참게 집단의 3번째(FRESHWATER no. 03) 개체 사이가 가장 먼 유전적 거리 (genetic distance=0.770)를 나타내었다. 위에서 언급했던 것처럼 RAPD-PCR 방법은 참게 및 꽃게 2종의 종 판별을 하기 위한 진단적 표지 (diagnostic marker)로 이용할 수 있는 잠재력을 가지고 있는 것으로 확인되었다.

• 한국어류학회지
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• 제18권1호
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• pp.1-11
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• 2006

RAPD 분석을 하기 위해서 우리나라 내륙의 충주지역과 서해에 인접한 당진지역에서 빙어 (Hypomesus nipponensis)의 두 지리적 집단으로부터 genomic DNA 를 분리 추출하였다. OPB-06, OPB-10, OPB-13, OPB-17, OPC-09, OPC-17 및 OPC-20의 7개 primer를 사용하여 shared loci, polymorphic 및 specific loci를 확인하였다. 충주 빙어집단에서는 한 primer 당 383개의 loci가 관찰되었고, 당진 집단에서는 287개의 loci가 확인되었다. 관찰된 loci 중에 23.8%에 해당되는 91개의 polymorphic loci가 충주 집단에서 확인되었고, 당진 집단에서는 47 (16.4%)개가 확인되었다. 각 집단에서 공유하는 loci의 수는 각각 충주 빙어집단에서 198개 그리고 당진 집단에서는 176개로 관찰되었다. 충주 빙어집단과 당진 집단에서는 각각 44개와 75개의 specific loci가 나타났다. 특히 두 집단이 공유하는 loci의 수는 99개로서 primer 당 평균 14.1개로 확인되었다. 두 빙어 집단의 bandsharing value의 평균값은 $0.700{\pm}0.008$로서 0.600에서 0.846의 범위를 나타내었다. 각각을 비교해 보면, 충주 집단에 속한 개체의 bandsharing value의 평균값이 당진 집단에서의 값보다 높게 나타났다. 7개의 primer를 사용하여 얻어진 dendrogram은 cluster 1 (CJ 01, 02, 03, 04, 05, 06, 07, 08, 09, 10 및 11), cluster 2 (DJ 01, 02, 03, 04, 05, 06, 07, 08 및 09) 및 cluster 3 (DJ 10 및 11)와 같이 3개의 유전적 클러스터로 나뉘어졌다. 두 집단의 유전적 거리는 0.040에서 0.545 사이로 나타났다. 따라서 RAPD-PCR 분석을 통해서 빙어 두 집단의 유의성이 있는 유전적 거리를 확인하였다.

• 한국발생생물학회지:발생과생식
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• 제23권3호
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• pp.297-304
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• 2019

The higher fragment sizes (>2,100 bp) are not observed in the two C. spp. populations. The six oligonucleotides primers OPA-11, OPB-09, OPB-14, OPB-20, OPC-14, and OPC-18 were used to generate the unique shared loci to each tonguesole population and shared loci by the two tonguesole populations. The hierarchical polar dendrogram indicates two main clusters: Gunsan (GUNSAN 01-GUNSAN 11) and the Atlantic (ATLANTIC 12-ATLANTIC 22) from two geographic populations of tonguesoles. The shortest genetic distance displaying significant molecular difference was between individuals' GUNSAN no. 02-GUNSAN no. 01 (genetic distance=0.038). In the long run, individual no. 02 of the ATLANTIC tonguesole was most distantly related to GUNSAN no. 06 (genetic distance=0.958). These results demonstrate that the Gunsan tonguesole population is genetically different from the Atlantic tonguesole population. The potential of PCR analysis to identify diagnostic markers for the identification of two tonguesole populations has been demonstrated. As a rule, using various oligonucleotides primers, this PCR method has been applied to identify polymorphic/specific markers particular to species and geographical population, as well as genetic diversity/polymorphism in diverse species of organisms.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2002년도 춘계 수산관련학회 공둥학술발표회
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• pp.279-280
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• 2002

Genomic DNA from the muscle of marsh clam (Corbicula leana) from Gochang was extracted in order to identify genetic differences and similarity by randomly amplified polymorphic DNAs-polymerase chain reaction. 3.28 of the 23.0 polymorphic bands per lane were found to be polymorphic in marsh clam. Also, about 4.34% of total polymorphic bands were either specific to marsh clam. The major common bands of 0.28 kb generated by primer OPB-15 (GGAGGGTGTT) were present in every individuals, respectively, which were polymorphic. This common bands which present in every individuals should be diagnostic of specific strains, species and/or their relatedness. Primer OPB-19 (ACCCCCGAAG) produced the highest number of specific bands, which was 12. The specific minor band of 0.07 kb was present in lane 22, which were polymorphic. Especially, only a specific band (1.35 kb) identifying individuals was observed in lane 22.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2002년도 춘계 한국양식학회 학술대회 발표요지
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• pp.171-172
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• 2002

Genomic DNA from the muscle of marsh clam (Corbicula leana)from Gochang was extrected in order to identify genetic differences and similarity by randomly amplified polymorphic DNAs-polymerase chain reaction. 3.28 of the 23.0 polymorphic bands per lane were found to be polymorphic in marsh clam. Also, about 4.34% of total polymorphic bands were either specific to marsh clam. The major common bands of 0.28 kb generated by primer OPB-15 (GGAGGGTGTT) were present in every individuals, respectively, which were polymorphic. This common bands which present in every individuals should be diagnostic of specific strains, species and-or their relatedness. Primer OPB-19 (ACCCCCGAAG) produced the highest number of specific bands, which was 12. The specific minor band of 0.07 kb was present in lane 22, which were polymorphic. Especially, only a specific band (1.35kg) identifying individuals was observed in lane 22.

• 한국패류학회지
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• 제25권1호
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• pp.5-13
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• 2009

Genomic DNA isolated from two geographical ark shell (Scapharca subcrenata) populations was amplified several times by PCR reactions. The ark shell population from Daecheon (ASPD) and from Wonsan (ASPW) in the West Sea and the East Sea of Korean Peninsula, respectively, obtained. The seven arbitrarily selected primers OPA-05, OPA-11, OPB-09, OPB-11, OPB-14, OPC-18 and OPD-07 were shown to generate the loci observed per primer, shared loci by each population, specific loci, unique shared loci to each population and shared loci by the two populations which could be clearly scored. Here, 862 loci were identified in the ASPD population, and 1,191 in the ASPW population: 137 specific loci (15.9%) in the Daecheon population and 84 (7.1%) in the Wonsan population. 407 shared loci by each population, with an average of 58.1 per primer, were observed in the ASPD population. 473 shared loci by each population, with an average of 67.6 per primer, were identified in the ASPW population. The numbers of specific loci in the ASPD and ASPW population were 137 and 84, respectively. Consequently, the average bandsharing value of individuals within the ASPW population was much higher than in the ASPD population. The bandsharing value between individuals' no. 08 and no. 13 was 0.628, which was the highest measured between the two geographical populations. The dendrogram obtained by the seven primers indicated three genetic clusters: cluster 1 (DAECHEON 01-DAECHEON 11), cluster 2 (WONSAN 12 and 14) and cluster 3 (WON SAN 13, 15, 16, 17, 18, 19, 20, 21 and 22). The genetic distance between the two geographical populations ranged from 0.043 to 0.499. Especially, individual no. 10 of Daecheon population was most distantly related to no. 14 of Wonsan population (genetic distance = 0.499).