• Journal of Embryo Transfer
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• v.29 no.3
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• pp.201-206
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• 2014

The objectives of the study were to determine an effective culture dish, culture duration and protein supplementation in medium for in vitro maturation (IVM) of oocytes of native zebu cows in Bangladesh. The ovaries of cows were collected from local slaughterhouse followed by aspiration of follicular fluid. The cumulus-oocyte-complexes (COCs) with more than 3 compact cumulus cell layers were cultured in tissue culture medium (TCM) 199 for maturation. The maturation of oocytes was determined by observing polar body under microscope. To determine an effective culture dish, 130 COCs derived from 48 ovaries in a well of 4-well dish and 102 COCs derived from 36 ovaries in drops covered with mineral oil within 35 mm petri dish were cultured for 24 hours. The rate of maturation of oocytes did not vary between 4-well dish ($51.3{\pm}15.0%$) and drops in petri dish ($52.4{\pm}11.6%$). To determine the effective culture duration, 185 COCs derived from 62 ovaries were cultured in drops for 18, 21, 24 and 27 hours. The rate of maturation of occytes ranged from $51.9{\pm}9.4%$ (18 hours) to $59.0{\pm}17.1%$ (27 hours) and the difference in maturation rate among different culture durations was not significant (P>0.05). To determine an effective protein supplementation, 63 oocytes from 19 ovaries were cultured separately in TCM 199 supplemented with either fetal bovine serum (FBS) or bovine serum albumin (BSA). The rate of maturation was significantly (P<0.01) higher in medium supplemented with FBS ($55.63{\pm}16.19%$) than that of BSA ($14.82{\pm}9.36%$). In conclusion, COCs of native zebu cows can be cultured for IVM either in 4-well culture dish or droplets in petri dish for 18 to 27 hours in medium supplemented with FBS.

• Korean Journal of Community Nutrition
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• v.5 no.2
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• pp.297-306
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• 2000

In order to examine the relationship between the number of different foods consumed and nutrient intake, one-day food consumption were surveyed by 24-hour recall from a sample of 287 individuals(20-49 years) living in Daejon City. The number of consumed food items did not include seasonings except red pepper power, sugar, oil, and soybean paste when used in large amounts(DVS). The number, including all seasonings except salt and vinegar(DVSS), was also counted. Of the total subjects, 43.6%(DVS) or 39.0%(DVSS) consumed 18-23 daily different foods with an average of 20.2 or 22.9, respectively. As the DVS increased, daily intakes of total foods and most food groups were elevated. Cereals however were not changed and eggs were decreased with increasing DVS. Energy and nutrient intakes and their mean adequacy ratio(MAR) and index of nutritional quality(INQ) also show positive correlations with DVS. MAR equal to or greater than($\geq$) 0.75 was taken as a cut-off point for nutritional adequacy. In that case, the mean INQ was shown to be $\geq$1. MRI(10) for energy and 9 nutrients and MAR(3) for Ca, vitamin A, and riboflavin were estimated. Form a regression analysis, when MAR(10) was 0.75, the DVS and DVSS were assumed to be 19.6 and 22.2, respectively. And when MAR(3) was 0.75, the DVS and DVSS were assumed to be 31.6 and 34.6, respectively. However the subjects whose MAR(3) was 0.75(0.7-0.8) 23.8(DVS) or 26.6(DVSS) different foods in average, and their mean intakes of energy and all nutrients, except vitamin A, were ranged at 85-100% of the RDA. Of the 74 subjects who consumed DVS$\geq$24, 24 to 42 numbers took 〈75% RDA of Fe, Ca, riboflavin, and vitamin A. And five of 7 who consumed DVS$\geq$32 took $\geq$125%RDA of protein, which showed concerns of overnutrition in case of DVS$\geq$32. Form the above results it could be suggested that a daily intake of 24-32(or 28) of DVS or 27-35(or 31) of DVSS was recommendable for an optimal nutritional of all nutrients if the variety of food groups and sufficient intake of vitamin A and calcium were emphasized together.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.2
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• pp.151-158
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• 2010

Camelina sativa L., known as popular names "gold-of-pleasure" or "false flax" is an alternative oilseed crop that can be grown under different climatic and soil conditions. Up to date, however, the genomic information of Camelina has not been studied in detail. Therefore, a cDNA library was constructed and characterized from young leaves. The constructed cDNA library incorporated of 1334 cDNA clones and the size of the insertion fragments average was 736 base pair. We generated a total of 1269 high-quality expressed sequence tags (ESTs) sequences. The result of cluster analysis of EST sequences showed that the number of unigene was 851. According to subsequent analysis, the 476 (55.9%) unigenes were highly homologous to known function genes and the other 375 (44.1%) unigenes were unknown. Remaining 63 (7.4%) unigenes had no homology with any other peptide in NCBI database, indicating that these seemed to be novel genes expressed in leaves of Camelina. The database-matched ESTs were further classified into 17 categories according to their functional annotation. The most abundant of categories were "protein with binding function or cofactor requirement (27%)", "metabolism (11%)", "subcellular localization (11%)", "cellular transport, transport facilities and transport routes (7%)", "energy (6%)", "regulation of metabolism and protein function (6%)". Our result in this study provides an overview of mRNA expression profile and a basal genetic information of Camelina as an oilseed crop.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.4
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• pp.325-332
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• 2017

This research was conducted to evaluate methods of enhancing the waterlogging resistance of soybean plant. Thus, we applied seven types of plant growth regulators (PGRs) to soybean plants and exposed them to waterlogged conditions for a total of 14 days. To evaluate stress resistance, we monitored plant growth characteristics data such as height, chlorophyll content, and chlorophyll fluorescence for 28 days after the initial waterlogging (14 days under waterlogging conditions and 14 days after waterlogging). According to the results, plant height was significantly increased by gibberellin A4 ($GA_4$) treatment compared to the control treatment and waterlogging-only treatment. However, we could not detect plant height owing to plant death when we applied abscisic acid (ABA). Except for $GA_4$ and ABA treatments, plant heights slightly decreased in all treatments compared to the waterlogging-only treatment. The chlorophyll content and chlorophyll fluorescence showed a similar tendency among PGR treatments. The chlorophyll content and chlorophyll fluorescence were significantly increased by ethephon and kinetin treatments 28 days after waterlogging compared to the waterlogging-only treatment. Consequently, kinetin and ethephon treatments induced more resistant phenotypes in soybean plants during or after exposure to waterlogging conditions.

• Journal of Korean Society of Environmental Engineers
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• v.34 no.9
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• pp.604-612
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• 2012

Self-management plan for GHG (Greenhouse Gas) reduction should be prepared in academic facilities, which occupy a large amount of energy consumption. In this study, a university was chosen as one of the major academic facilities and its energy consuming pattern and GHG emission were analyzed. The results have shown that annual $CO_2$ emission from university buildings was 10,452 ton-$CO_2$ (0.65 ton-$CO_2/m^2$), and dependent upon 78.0% electricity, 20.5% LNG and 1.5% oil, respectively as energy sources. According to more detail analysis by usage of energy consumption, appliances occupies 36.7% followed by gas heating (18.9%), lighting (18.6%), heating with electricity (12.5%), cooling with electricity (10.2%), transportation (1.5%), gas cooling (1.2%) and cooking (0.4%). Furthermore, annual $CO_2$ emissions per unit area and a student by electricity usage were evaluated to 51.30 kg-$CO_2/m^2$ and 981.86 kg-$CO_2$/capita, respectively and those by LNG usage were 14.61 kg-$CO_2/m^2$ and 241.01 kg-$CO_2$/capita.

• Journal of Korean Society of Environmental Engineers
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• v.31 no.7
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• pp.531-540
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• 2009

In this study, thermo-gravimetric analysis(TGA) was used to investigate the effect of urea concentration and heating rate on the ammonia($NH_3$) formation process from urea solution. A newly designed pipe nozzle was inserted through a 1,000 N${\ss}$(C)/h oil firing boiler to compare the DeNOx efficiencies between the upward and downward nozzle. This experiment reveals the effect of path which an urea droplet goes through. Urea solution showed the same TGA graph without regard to the presence of oxygen. Heating rate had a great influence on the weight loss trend. But the concentration of urea solution between 10% and 40% did not affect so much the thermal decomposition temperature. Therefore, heating rate is more important factor on the thermal decomposition of urea than the concentration of urea solution. Three nozzles located at different positions showed similar DeNOx efficiencies such as 68.1%, 71.8%, 70.8% at the same temperature. Even though urea solution was injected for the same zone, the injection direction made much difference in DeNOx efficiency. A upward nozzle showed 68.1% and downward nozzle 9.5%. This results illustrate the importance of heating rate.

• Economic and Environmental Geology
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• v.47 no.6
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• pp.673-681
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• 2014

Recently, many countries in the world try to develop alternative energy sources, however, traditional hydrocarbon resources are still occupying most of the energy resources. Exploration demands for high technologies are increasing in the development of limited oil & gas resources field owing to the exhaustion of hydrocarbon resources for access area. Therefore, an effort for the development and the application of new technologies such as azimuth seismic survey, ocean-bottom seismic survey and marine controlled-source electromagnetic survey is necessary as well as an understanding of the existing technologies such as 2D/3D seismic survey. This dissertation is designed with the purpose of introducing marine hydrocarbon exploration technologies and analyzing their internalexternal researches, development and science information. In this study, we analised total 616 dissertations for the marine petroleum exploration released in the Sci-expanded DB of 'web of science' during the 2001~2014 periods.

• Korean Journal of Environmental Agriculture
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• v.23 no.4
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• pp.264-271
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• 2004

A three-dimensional numerical mesoscale model by Pielke's estimation (University of Virginia Mesoscale Model, UVMM) was applied to investigate the effects of land characteristics including land-humidity, land-roughness and land-albedo on some micro-climatic coefficients and the air cooling capacity. The results indicated that land-characteristics exposed a significant effect on air cooling. Air cooling effects between in urban and agricultural areas were compared and the effects were much higher in agricultural area. Air cooling effects of weed species were different and when converted into economic values by diesel oil price the effects were ranged from 411 to 816 Won/plant.

• Korean Journal of Environmental Agriculture
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• v.30 no.4
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• pp.466-472
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• 2011

BACKGROUND: Soybean [Glycine max (L.) Merrill] is a legume and an important oil crop worldwide. This study was conducted to evaluate the possible impact of transgenic soybean cultivation on the soil microbial community. METHODS AND RESULTS: Microorganisms were isolated from the rhizosphere soils. Microbial community was identified based on the culture-dependent and molecular biology methods. The total numbers of bacteria, fungi, and actinomycete in the rhizosphere soils cultivated with transgenic and non-transgenic soybeans were similar to each other, and there was no significant difference between transgenic and non-transgenic soybeans. Dominant bacterial phyla in the rhizosphere soils cultivated with transgenic or non-transgenic soybeans were Actinobacteria, Firmicutes, and Proteobacteria. The microbial communities in transgenic and non-transgenic soybean soils were characterized using the denaturing gradient gel electrophoresis (DGGE). The DGGE profiles showed the different patterns, but didn't show significant difference to each other at 0.05 significance level. DNAs were isolated from soils cultivating transgenic or non-transgenic soybeans and analyzed for persistence of transgenes in the soil by using PCR. PCR analysis revealed that there were no amplified ${\gamma}$-tmt and bar gene in soil DNA. CONCLUSION(S): The results of this study suggested that microbial community of soybean field were not significantly affected by cultivation of the transgenic soybeans.

• Korean Journal of Environmental Biology
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• v.27 no.1
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• pp.31-39
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• 2009

To recognize the species composition and community of chironomid midge in agroecosystem and evaluate removal capacity of organic matter using the dominant species among them. Chironomid midge and invertebrate cohabitants were quantitatively collected at 78 representative stations of five habitat types (in arable land during 2004$\sim$2006) A, paddy fields of the large scale arable land; B, paddy fields of the environmental friendly arable land; C, influent of domestic waste water; D, influent of waste water by livestock; E, the irrigation ditch of paddy area. The total sixteen species of chironomid midges at arable land were presented. And also chironomid midges were recognized by five habitat types: eleven species in D, eight in A, seven in E, six in B, and two in C. We confirmed dominant species in each habitat types as followings: Chironomus nipponensis in A, C, and D; Cricotopus sylvestris in E; Tanytarsus seosanensis in B. Water quality and community index were high in E, but low in A, B, C, and D. Comparing with non-treatment, removal activities of organic matter in bottom by C. nipponensis were increased 18% in 90 individuals treatment.