• Title/Summary/Keyword: OG

검색결과 672건 처리시간 0.03초

Analysis of Structure and Expression of Grapevine 2-oxoglutarate Oxygenase Genes in Response to Low Temperature

  • Kim, Seon Ae;Ahn, Soon Young;Yun, Hae Keun
    • 원예과학기술지
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    • 제34권1호
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    • pp.46-54
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    • 2016
  • 2-Oxoglutarate (2OG) acts as a signaling molecule and plays a critical role in secondary metabolism in a variety of organisms, including plants. Six 2-oxoglutarate (2OG) and Fe(II) oxygenase (2OGO) genes, VlCE2OGO1 [Vitis labruscana 2-oxoglutarate (2OG) and Fe(II) oxygenase 1], VlCE2OGO2, VlCE2OGO3, VlCE2OGO4, VlCE2OGO5, and VlCE2OGO6, which show different expression patterns upon transcriptome analysis of 'Campbell Early' grapevine exposed to low temperature for 4 weeks, were analyzed for their structure and expression. Comparison of the deduced amino acid sequences of the 2OGO genes from the V. labruscana transcripts revealed sequence similarities of 38.6% (VlCE2OGO1 and VlCE2OGO2) to 19.2% (VlCE2OGO2 and VlCE2OGO3). The lengths of these genes ranged from 1053 to 2298 bp, and they encoded 316 to 380 amino acids. The prediction of the secondary structure of the encoded proteins by Self-Optimized Prediction Method with Alignment (SOPMA) indicated that all the genes contained alpha helix (23.95 to 41.71%), extended strand (16 to 22.34%), beta turn (6.65 to 9.22%), and random coil (32.97 to 51.58%) in the analysis. Specific primers from unique regions in each gene obtained by alignment of nucleotide sequences were used in real time PCR for analysis of gene expression. All tested genes showed differential expression in grapevines exposed to low temperature. Of the six transcripts, VlCE2OGO1, VlCE2OGO2, and VlCE2OGO3 were up-regulated and VlCE2OGO4, VlCE2OGO5, and VlCE2OGO6 were down-regulated in response to cold treatments at all tested time points. The 2OG genes can be used for elucidation of mechanisms of tolerance to cold and as valuable molecular genetic resources for selection in breeding programs for cold-hardy grapevines.

Intracranial Aneurysms in the 3rd and 4th Decades in Comparison with Those in the 8th and 9th Decades

  • Kim, Chang-Hyun;Park, Seong-Hyun;Park, Jae-Chan;Hwang, Jeong-Hyun;Sung, Joo-Kyung;Hamm, In-Suk
    • Journal of Korean Neurosurgical Society
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    • 제38권1호
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    • pp.28-34
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    • 2005
  • Objective : This study is performed to compare older with younger groups about clinical characteristics and overall outcome of treatments for the intracranial aneurysms. Methods : We retrospectively investigated 633 patients with cerebral aneurysms who were admitted to our institute from January 2000 to May 2004. The authors divided the patients of cerebral aneurysm into two groups, one the third, fourth decades and the other eighth, ninth decades, analyzed clinical characteristics and overall outcome of treatments. Results : There were 57 patients [9.0%] under 39years old and 58 patients [9.2%] over 70. The female to male sex ratio was 0.5 : 1 in the younger group[YG] and 7.3 : 1 in the older group[OG], showing a female predominance with increasing age. In the YG, aneurysms were found in anterior communicating artery[A-com] [44.8%], middle cerebral artery [31.0%]. In the OG, aneurysm of posterior communicating artery [30.1%] was most common followed by that of A-com [26.9%]. More smokers and alcoholics were found in the YG. Older age was related to poor Hunt-Hess grade, Fisher's grade on admission, high incidence of unruptured aneurysms, and endovascular surgery. There was a higher prevalence of hypertension, intraventricular hematoma, hydrocephalus, and rebleeding in the preoperative state in the OG and postoperative complications including hydrocephalus, subdural fluid collection, and systemic complications. Overall outcome was poorer with advancing age [p=0.01]. Conclusion : The patients with aneurysms in the YG have distinct characteristics compared to those in the OG. Because of a good clinical grade on admission, a thin subarachnoid clot, and Low incidence of perioperative complications, the overall outcomes of the young patients were better than those of the old patients.

Intra-Oral Factors Influencing Halitosis in Young Women

  • Shon, Ho Sun;Kim, Kyoung Ok;Jung, Jae Kwan;Cha, Eun Jong;Lee, Su Ok;Kim, Kyung Ah
    • Osong Public Health and Research Perspectives
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    • 제9권6호
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    • pp.340-347
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    • 2018
  • Objectives: The aim of this research was to determine intra-oral factors that affect halitosis in young women. Methods: This study was performed between March 2014 to May 2014, and included 35 women in their 20s with good oral health. Correlation and logistic regression analyses were performed to investigate the change in halitosis immediately, and 1 hour after scaling. Results: In both oral gas (OG) and extraoral gas (EG) groups, halitosis was reduced after scaling compared to before scaling. The logistic regression analysis of oral state factors in OG showed that as oral fluid [odds ratio (OR) = 0.792, p = 0.045] and dental plaque (OR = 0.940, p = 0.016) decreased by 1 unit, the OR in the OG group decreased (> 50). In addition, as glucose levels in the oral cavity (OR = 1.245, p = 0.075) and tongue coating index (OR = 2.912, p = 0.064) increased by 1 unit, the OR in the OG group increased (> 50). Furthermore, in the EG group, as oral fluid (OR = 0.66, p = 0.01) and dental plaque (OR = 0.95, p = 0.04) decreased, the OR in the EG group decreased (> 50) significantly. Conclusion: To control halitosis, it is necessary to increase oral fluid and decrease the amount of tongue plaque. Furthermore, maintaining a healthy oral environment, aided by regular scaling and removal of dental plaque, may significantly control halitosis.

Structural Stability of Bacteriorhodopsin Solubilized by Triton X-100

  • Sasaki, Takanori;Sonoyama, Masashi;Mukai, Yuri;Nakazawa, Chieko;Mitaku, Shigeki
    • Journal of Photoscience
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    • 제9권2호
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    • pp.293-295
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    • 2002
  • The structural stability of bacteriorhodopsin (bR) solubilized by Triton X-100 (TX-100) was studied by measuring the denaturation kinetics in the dark and under illumination, and compared with the structural stability of bR solubilized by octyl-${\beta}$-glucoside (OG). In the dark, bR solubilized by TX- 100 was more stable than bR solubilized by OG. Under illumination, bR solubilized by TX-100 showed light-induced denaturation in the same manner as bR solubilized by OG. These results in the dark well correlated with the experimental results of the visible CD band. Although solubilized bR in the TX-100 concentration range of 2-50 mM showed almost identical positive CD band and did not denature in the dark at 35$^{\circ}$C, the kinetic constant of the photobleaching increased with the increase of TX-100 concentration. These results suggested that photo-intermediates of solubilized bR are destabilized by TX-100 micelles.

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외안근전침요법과 한약을 이용한 Tolosa-Hunt Syndrome 치험 1례 (A Case of Tolosa-Hunt Syndrome Treated with Herbal Medicine and Electroacupuncture at Oculomotor Muscles)

  • 윤인환;강정아;김남권
    • 대한한의학회지
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    • 제29권3호
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    • pp.155-160
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    • 2008
  • Tolosa-Hunt syndrome (THS) is characterized by painful ophthalmoplegia and diagnosed by the exclusion of other major diseases. This study was done to find the effectiveness of electroacupuncture at oculomotor muscles and herbal-med on a THS patient. We used this method and Bogan-san ($B\check{a}og\check{a}n-s\check{a}n$) without steroids and treated a THS patient who suffered from pain behind the right eye, ophthalmoplegia, ptosis, frontal headache and dysosmia. 18 days after admission, the symptoms were improved and almost gone. In conclusion, we suggest electroacupuncture at oculomotor muscles and Bogan-san ($B\check{a}og\check{a}n-s\check{a}n$) is effective to treat THS without steroids, and we recommend more studies of this method on THS.

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Diurnal Changes in the Distribution of Ruminal Bacteria Attached to Feed Particles in Sheep Fed Hay Once Daily

  • Pan, J.;Suzuki, T.;Ueda, K.;Tanaka, K.;Okubo, M.
    • Asian-Australasian Journal of Animal Sciences
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    • 제13권12호
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    • pp.1708-1716
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    • 2000
  • A study was made of diurnal changes in the ruminal bacteria associated with feed particles, i.e., non-associated (NAB), loosely associated (LAB), and tightly associated with particles (TAB), and the TAB concentration in different particle sizes from sheep fed orchardgrass (OG) or alfalfa (ALF) hay. Diaminopimelic acid (DAPA) was used to determine the TAB mass. Results showed that the bacterial masses in NAB and LAB were small, but comprised over 90% in TAB. The TAB mass in the ALF group sharply increased within 2 h after feeding and decreased afterward. The TAB mass showed the same trend in the OG group, increasing from 0 h to 2 h, but remained at the same level up to 14 h after feeding. The peak bacterial mass was, however, lower in the OG than the ALF group. The TAB concentration reflected the changes in total particulate tightly associated bacterial masses in both groups of hay fed sheep. Number of bacterial colonies per particle increased as the particulate size decreased in both groups. This difference, however, tended to decline as the postprandial period was prolonged. DAPA, however, tended to overestimate the TAB mass in the reticulo-rumen digesta of the hay fed sheep.

패키징으로 인한 응력이 MEMS 소자에 미치는 영향 분석 및 개선 (Effects of Package Induced Stress on MEMS Device and Its Improvements)

  • 좌성훈;조용철;이문철
    • 한국정밀공학회지
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    • 제22권11호
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    • pp.165-172
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    • 2005
  • In MEMS (Micro-Electro-Mechanical System), packaging induced stress or stress induced structure deformation becomes increasing concerns since it directly affects the performance of the device. In the decoupled vibratory MEMS gyroscope, the main factor that determines the yield rate is the frequency difference between the sensing and driving modes. The gyroscope, packaged using the anodic bonding at the wafer level and EMC (epoxy molding compound) molding, has a deformation of MEMS structure caused by thermal expansion mismatch. This effect results in large distribution in the frequency difference, and thereby a lower yield rate. To improve the yield rate we propose a packaged SiOG (Silicon On Glass) process technology. It uses a silicon wafer and two glass wafers to minimize the wafer warpage. Thus the warpage of the wafer is greatly reduced and the frequency difference is more uniformly distributed. In addition. in order to increase robustness of the structure against deformation caused by EMC molding, a 'crab-leg' type spring is replaced with a semi-folded spring. The results show that the frequency shift is greatly reduced after applying the semi-folded spring. Therefore we can achieve a more robust vibratory MEMS gyroscope with a higher yield rate.

Laparoscopic Gastrectomy Performed by an Expert in Open Gastrectomy

  • Chi, Kyong-Choun;Park, Joong-Min
    • Journal of Gastric Cancer
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    • 제17권3호
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    • pp.237-245
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    • 2017
  • Purpose: Senior surgeons prefer open gastrectomy (OG), while young surgeons prefer laparoscopic gastrectomy (LG). The purpose of this study was to evaluate the surgical outcomes of LG performed by a senior surgeon who was an expert in OG during his learning period, by comparing them with LGs performed by a young surgeon. Materials and Methods: A senior surgeon performed 50 curative gastrectomies with laparoscopy (LG-S group) from March 2015 to August 2016. A young surgeon's initial 50 LGs comprised the LG-Y group. Clinicopathological characteristics and surgical outcomes were compared between the LG-S and LG-Y groups. Results: D2 lymphadenectomy was more frequently performed in the LG-S group than in the LG-Y group (P=0.029). The operation time and number of retrieved lymph nodes did not significantly differ between the 2 surgeons (P=0.258 and P=0.410, respectively). Postoperative hospital stay and postoperative complication rate were similar between 2 groups (P=0.234 and P=1.000, respectively). Similarly, significant decreases in operation time with increasing case numbers were observed for both surgeons, whereas the number of retrieved lymph nodes increased significantly in the LG-Y group but not in the LG-S group. Conclusions: The LG outcomes when performed by the senior surgeon were comparable to those when performed by the young surgeon, despite performing more extended lymphadenectomies. Senior surgeons who are experts in OG should not refrain from performing LG.

석유화학공장 방류수내 난분해성 유기물의 Fenton 산화처리 (Fenton난s Reagent Oxidation of Refractory Organics in Petrochemical Plant Effluent)

  • 이규훈;정대영;박태주
    • 한국환경과학회지
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    • 제5권1호
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    • pp.51-59
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    • 1996
  • The purpose of this study was to evaluate the partial oxidation of the biological treatment plant effluents using Fenton's reagent as a pretreatment step prior to a tertiary biological oxidation of these effluents. Fenton's reagent was evaluated as a pretreatment process for inhibitory or refractory organics. Based on the Fenton oxidation system, the petrochemical wastewater treatment plant effluent was shown to have significant improvement in toxicity after oxidation with hydrogen peroxide. For example, at ranee of 42 ∼ 184 mg/L COD of petrochemical plant effluents, the COD removal efficiencies were from 38.2% to 60.1% after reaction with hydrogen peroxide 200 mg/L and Fe2+ 100 mg/L and reaction time was 30 minutes. The total TOC reduction were about 15.8∼22.4% with same test condition and difference between the overall removal rate and BOD/COD ratio after Fenton's oxidation estabilished in the biodegradation and otherwise meets the discharge standard or reuse for cooling tower make-up water.

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4세대 이동통신용 이중접지 내장형 광대역 안테나의 설계와 제작에 관한 연구 (A Study on the Design and Fabrication of a Dual-Ground and Broad-band Internal Antenna for 4th-Generation Mobile Communications)

  • 박정률;최병하;공진우;윤현수;김규철
    • 한국항행학회논문지
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    • 제12권2호
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    • pp.100-108
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    • 2008
  • 본 논문에서는 4세대 이동통신용 이중접지 내장형 고이득, 광대역 안테나를 설계 및 제작하였다. 최적으로 설계된 안테나는 공기층에 의해 분리되어진 방사패치와 접지면의 유동에 의한 안테나의 파손을 막기 위해서 foam(${\varepsilon}_r{\fallingdotseq}1.03$)으로 고정하였으며, 방사패치는 0.05 [mm] 두께의 도체를 사용하였고, Ground는 FR4 기판을 사용하였다. 최적 설계된 안테나는 그 제작 방법이 간단하여 인쇄회로 기판은 사진 식각법으로 동 테이프는 수작업으로 제작하였다. 제작된 안테나의 입력 반사손실 -10 [dB]이하 (VSWR $$\leq_-$$ 2)를 기준으로 3499 ~ 4743 [MHz]까지 1244[MHz]라는 광대역 특성을 나타내었다. 3400, 3600, 3800, 4000, 4200[MHz]에 대하여 방사패턴을 측정한 결과 무지향성의 특성을 얻을 수 있었으며, E-plane에서 4.7 ~ 6.1 [dBi], H-plane 에서 2.1 ~ 4.3 [dBi]라는 높은 이득 결과를 얻을 수 있었다.

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