• Journal of the Korean Society of Marine Environment & Safety
• /
• v.16 no.2
• /
• pp.141-151
• /
• 2010

Harmful algal blooms (HABs), commonly known as red tides, are aquatic phenomena caused by the rapid growth and accumulation of certain microalgae, which can lead to marked discoloration of surface waters, and severe impacts on public health, commerce, and the environment. In South Korea, the red tides have been a serious and recurrent problem, especially along the south coast. Plenty of yellow loess was used to control an outbreak of the red tides for 15 years from 1996 until now. Yellow loess was almost sprayed in the vicinity of a large fish farming industry. In this research, the distribution characteristics and density distribution of zooplankton were investigated in autumn (Oct. 2008) and spring (Apr. 2009) using volume backscattering strength (SV) calculated by the zooplankton collected with north pacific standard (NORPAC) net and the echo intensity measured with ADCP at stations on the study area in the spraying ocean of yellow loess (SOYL), and the non-spraying ocean of yellow ocean (NOYL) by the red tide generating every year. The species number and the individuals per unit volume of the zooplankton collected in NOYL was high and it which was collected in SOYL was low. As a result of comparing the volume backscattering strength ($SV_c$) calculated by species and length of the zooplankton collected with NORPAC net with the volume backscattering strength ($SV_m$) calculated by the echo intensity measured with ADCP at stations on the study area, although $SV_c$ and $SV_m$ of NOYL were generally in agreement, $SV_m$ of SOYL was higher than $SV_c$ 4.3dB, i.e. ADCP is greatly influenced by suspended solid in SOYL. The horizontal distribution map of $SV_m$ at the study area in autumn (Oct. 2008) and spring (Apr. 2009) was drawn. $SV_m$ of SOYL is higher than NOYL and autumn is higher than spring. $SV_m$ can suppress the overestimate or underestimate of $SV_c$.

• Reproductive and Developmental Biology
• /
• v.34 no.3
• /
• pp.229-233
• /
• 2010

Pluripotency and self-renewal capacity of human embryonic stem cells (hESCs) are retained by hESCs related genes as OCT4, SOX2 and NANOG. These genes are shown high expression level in diverse cancer cells and have potential role in the carcinogenesis. On the contrary to this, several genes which are up-regulated in the differentiated hESCs are involved to suppress the carcinogenesis or proliferation of cells. We discovered several genes in immortalized lung fibroblast (WI-38 VA13) by suppression subtractive hybridization. Among them, we focused chromosome 6 open reading frame 62 (C6orf62) which is uncharacterized, mapped to 6p22.3 and generated to Hepatitis B virus X-transactivated proteins (HBVx-transactivated proteins, XTP). Aim of this study was to characterize C6orf62 through analyzing of expression pattern in various cell lines. Expression of C6orf62 was significantly upregulated in diverse normal cell lines than cancer cell lines. And C6orf62 was up-regulated in differentiated hESCs (endothelial cells, neural cells) compared to those of undifferentiated hESCs. Also, C6orf62 in WI-38 cells was highly up-regulated during G1/S transition of the cell cycle. Taken together, C6orf62 is shown expression pattern similar to differentiated hESCs-associated genes which down-regulated in cancer cells. Therefore, we assume that C6orf62 may participate to suppress the proliferation and to induce differentiation through regulating the cell cycle.

• Reproductive and Developmental Biology
• /
• v.35 no.1
• /
• pp.67-75
• /
• 2011

The faulty regulation of imprinting gene lead to the abnormal development of reconstructed embryo after nuclear transfer. However, the correlation between the imprinting status of donor cell and preimplantation stage of embryo development is not yet clear. In this study, to determine this correlation, we used the porcine spermatogonial stem cell (pSSC) and fetal fibroblast (pFF) as donor cells. As the results, the isolated cells with laminin matrix selection strongly expressed the GFR ${\alpha}$-1 and PLZF genes of SSCs specific markers. The pSSCs were maintained to 12 passages and positive for the pluripotent marker including OCT4, SSEA1 and NANOG. The methylation analysis of H19 DMR of pSSCs revealed that the zinc finger protein binding sites CTCF3 of H19 DMRs displayed an androgenic imprinting pattern (92.7%). Also, to investigate the reprogramming potential of pSSCs as donor cell, we compared the development rate and methylation status of H19 gene between the reconstructed embryos from pFF and pSSC. This result showed no significant differences of the development rate between the pFFs ($11.2{\pm}0.8%$) and SSCs ($13.3{\pm}1.1%$). However, interestingly, while the CTCF3 methylation status of pFF-NT blastocyst was decreased (36.3%), and the CTCF3 methylation status of pSSC-NT blastocyst was maintained. Therefore, this result suggested that the genomic imprinting status of pSSCs is more effective than that of normal somatic cells for the normal development because the maintenance of imprinting pattern is very important in early embryo stage.

• Journal of Korean Academy of Nursing
• /
• v.14 no.1
• /
• pp.69-82
• /
• 1984

The general fearfulness of hospitalized school-age children and the emotional anxiety of their mothers were investigated and compared to those of normal children and their mothers, in order to provide theoretical basis for establishing comprehensive nursing care of hospitalized children including their mothers. The present study was carried out from Sept. 5th to Oct. 3rd, 1983. A total of 81 hospital I zed children and their mothers were investigated and 95 normal elementary school children and their mothers as control group were surveyed The general fearfulness of children and the emotional anniety of their mothers were measured through questionaire by using Geer's FSS-Ⅱscale and Spielberger's STAI scale, respectively. The results were analyzed by computer using S.P.S.S. program and summarized as follows: 1. The emotional anxiety of the mothers of hospitalized children was greater than that of normal children's mothers. (P=0.000). 2. The general fearfulness of hospitalized children was greater than that of normal children (p=0.000) 3. The general fearfulness of hospitalized children varied with children's age and sex, mother's age and mother's experience of hospitalization. a) The general fearfulness of the hospitalized children at the age of 7∼9 was greater than that of the age 10∼12. (P=0.020) b) Girl's scored greater fearfulness than boys. (p=0.037) c) The younger mother's age resulted in the higher children fearfulness. (P= 0.0059). d) When the mothers did not have experience of hospitalization, the children's fearfulness was high. (P=0.014) 4. The anxiety-state of hospitalized children's mother was proportionally reacted to their anxiety-trait. (r=0.694, p=0.000) 5. The relationship between mother's emotional anxiety and their hospitalized children's general fearfulness failed to show statistical significance. (r= 0.1184, P＞0.05). These results indicated that the general fearfulness of hospitalized children was affected by environmental factors beside mother's emotional anxiety. An extensive study on the factors influencing the general fearfulness of hospitalized children is needed.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.47 no.6
• /
• pp.945-959
• /
• 2014

This study was conducted to provide basic data for optimal fisheries management through the accurate understanding the catches, fishing level, species composition, distribution characteristics and fisheries status of trammel net in Gangwon Province. As the results of trammel net survey, Pisces were collected 77 species, 1,983.0 kg, Crustacea 9 species, 569.0 kg, Cephalopoda 5 species, 75.3 kg, Gastropoda 12 species, 16.5 kg in the coastal waters of Sokcho and Pisces 55 species, 3,681.5 kg, Crustacea 6 species, 2,229.8 kg, Gastropoda 10 species, 72.8 kg, Cephalopoda 4 species, 10.1 kg in the coastal waters of Donghae, respectively. The catches of important species with season and depths showed that the main target species is Pleuronectidae spp. at all depth and Aptocyclus ventricosus at <100 m in Sokcho, Liparis spp. and Dasycottus setiger at 200-500 m in Donghae, respectively. The mean total length (cm) of Gadus macrocephalus and Aptocyclus ventricosus didn't appear significant difference, but the mean total length of Pleuronectidae spp., Liparis spp. and D. setiger in Donghae is bigger than in Sokcho. From the cluster and MDS analysis based on Bray-Curtis similarity matrix of fourth root transformed, catches data of dominant species in the coastal waters of Sokcho and Donghae was divided into three different groups of the demersal organisms community in 12 survey of Donghae (Group A) and Jul., Oct.-Dec. survey of Sokcho (Group B) and Jan.-Jun. and Aug.-Sep. survey of Sokcho (Group C).

• The Korean Journal of Physiology and Pharmacology
• /
• v.26 no.5
• /
• pp.367-375
• /
• 2022

Gastric cancer stem cells (GCSCs) are a major cause of radioresistance and chemoresistance in gastric cancer (GC). Therefore, targeting GCSCs is regarded as a powerful strategy for the effective treatment of GC. Atorvastatin is a widely prescribed cholesterol-lowering drug that inhibits 3-hydroxy-3-methylglutaryl-coenzyme A reductase, a rate-limiting enzyme in the mevalonate pathway. The anticancer activity of atorvastatin, a repurposed drug, is being investigated; however, its therapeutic effect and molecular mechanism of action against GCSCs remain unknown. In this study, we evaluated the anticancer effects of atorvastatin on MKN45-derived GCSCs. Atorvastatin significantly inhibited the proliferative and tumorsphere-forming abilities of MKN45 GCSCs in a mevalonate pathway-independent manner. Atorvastatin induced cell cycle arrest at the G0/G1 phase and promoted apoptosis by activating the caspase cascade. Furthermore, atorvastatin exerted an antiproliferative effect against MKN45 GCSCs by inhibiting the expression of cancer stemness markers, such as CD133, CD44, integrin α6, aldehyde dehydrogenase 1A1, Oct4, Sox2, and Nanog, through the downregulation of β-catenin, signal transducer and activator of transcription 3, and protein kinase B activities. Additionally, the combined treatment of atorvastatin and sorafenib, a multi-kinase targeted anticancer drug, synergistically suppressed not only the proliferation and tumorsphere formation of MKN45 GCSCs but also the in vivo tumor growth in a chick chorioallantoic membrane model implanted with MKN45 GCSCs. These findings suggest that atorvastatin can therapeutically eliminate GCSCs.

• BMB Reports
• /
• v.51 no.2
• /
• pp.92-97
• /
• 2018

B cell leukemia/lymphoma 3 (Bcl3) plays a pivotal role in immune homeostasis, cellular proliferation, and cell survival, as a co-activator or co-repressor of transcription of the $NF-{\kappa}B$ family. Recently, it was reported that Bcl3 positively regulates pluripotency genes, including Oct4, in mouse embryonic stem cells (mESCs). However, the role of Bcl3 in the maintenance of pluripotency and self-renewal activity is not fully established. Here, we report the dynamic regulation of the proliferation, pluripotency, and self-renewal of mESCs by Bcl3 via an influence on Nanog transcriptional activity. Bcl3 expression is predominantly observed in immature mESCs, but significantly decreased during cell differentiation by LIF depletion and in mESC-derived EBs. Importantly, the knockdown of Bcl3 resulted in the loss of self-renewal ability and decreased cell proliferation. Similarly, the ectopic expression of Bcl3 also resulted in a significant reduction of proliferation, and the self-renewal of mESCs was demonstrated by alkaline phosphatase staining and clonogenic single cell-derived colony assay. We further examined that Bcl3-mediated regulation of Nanog transcriptional activity in mESCs, which indicated that Bcl3 acts as a transcriptional repressor of Nanog expression in mESCs. In conclusion, we demonstrated that a sufficient concentration of Bcl3 in mESCs plays a critical role in the maintenance of pluripotency and the self-renewal of mESCs via the regulation of Nanog transcriptional activity.

• Korean Journal of Occupational Health Nursing
• /
• v.8 no.1
• /
• pp.5-21
• /
• 1999

The purpose of this study is to evaluate the physical fitness status and health promoting life styles of the workers. For the purpose, 108 workers who attended the exercise program in Inchon branch of KISCO were selected as the subjects. From Oct. 20th to Nov. 30th, in 1998, they were firstly assessed their physical fitness. The items include cardio-respiratory endurance, flexibility, muscular strength, muscular endurance, agility, power, balance, body composition, etc. Secondly, the health promoting life styles were asked by questionnaires about daily life and dietary habits. Both of them were evaluated by 5 or 3 levels as A(very good) to E(very poor) or A (good) to C(poor). Those data were analyzed percentile, mean, standard deviation by SAS program. Major findings are as follows ; 1. The health promoting life styles were generally good, but 43.5% of the subjects didn't exercise at all. Most of them(93.5%) thought about their physical fitness status as lower than average level. About half of them(48.1%) didn't drink alcohol, non smokers were 70.4% of them. But they had poor dietary habits(lower than average level : 79.6%), females were a little bit better than males. The aged group had the poor body compositions, 21.4% of females and 10.0% of males were obese. 2. Physical fitness status of the workers were assessed as two areas, one is health related, the other is physical function related area. In the health related area, females were better than males, in view of age, forties aged group had the highest scores of all items except cardio-respiratory endurance. Among 'A' and 'B' level, muscular endurance was showed most frequently, followed by muscular strength, flexibility, cardiorespiratory endurance. In physical function related status, balance was ranked highly in the portion of over 'B', followed by power, agility. In view of sex, males were better than females for all items except balance, and there were various figures in the status by age groups. 3. Comprehensive assessment scores were poor(under 'D' leves were most frequent), females were better than males, and teenage group had the worst scores. In ages of the physical fitness, generally they had 1 year under their real ages, and females were better than males. In view of age, forties aged group was ranked highly and teenagers had lowest scores.

• Journal of Acupuncture Research
• /
• v.18 no.6
• /
• pp.1-13
• /
• 2001

Objective : To broaden understanding about relationship between Meridian Tendino-musculature and muscles in a lumbar area and to evaluate the effect of Meridian Tendino-musculature acupuncture and release therapy on acute lumbar sprain. Materials and Methods : From Oct. 1st, 2000 to Mar. 31th, 2001, 692 outpatient's chart of Kyung Won University Hospital were reviewed. Out of them, 39 outpatients were selected. they had low back pain, were diagnosed with acute lumbar sprain, showed only straightened curvature on lateral view of lumbar spine X-ray and get the Tendino-musculature acupuncture and release therapy. Results : 1. On patients' first visit, 72% had GrIII and Gr.IV predominantly. 2. Major muscles related with low back pain were divided into two groups. One was Quadratus lumborum group and the other was Rectus abdominis group. In the correlation with Meridian Tendino-musculature, the former was mainly related with Chok-taeyang(B) and slightly related with Chok-soyang(G), Chok-taeum(SP), the latter was mainly related with Chok-taeyum(SP), Chok-yangmyong(S) and slightly related with Chok-taeyang(B), Chok-soyang(G). 3. In the evaluation of treatment effect, Exellent was 27(69%), Good was 10(6%), Fair was 2(5%) and Bad was 0(0%). Fair rate reached 100% eventually. 4. Most of Gr.Ⅲ, Gr.Ⅳ patients who had severe conditions that almost every R.O.M. was limited and Milgram test positive was shown on the physical examination, were fully recovered and lived normal daily life without admission by Meridian Tendino-musculature acupuncture and release therapy within 3 to 5, 4 to 7 days, respectively, since they had started to get their outpatient treatment. 5. Two patients was troubled with pain induced, by twitching response and acupuncure stimuli, which lasted around acupunctured muscle for about a day after treatment, so they coudn't endure the pain and quitted treatment in spite of Fair condition. conclusion : Meridian Tendino-musculature acupuncture and release therapy was found to be helpful to patients who wish to recover from their back pain induced by acute lumbar sprain as soon as possible, but the treatment actually had some problems to be overcome such as pain during or after the treatment. therefor, in order to make this treatment method more available, we should pay more attention to improving treatment appliance and acupuncture technique.

• Clinical and Experimental Reproductive Medicine
• /
• v.31 no.4
• /
• pp.261-271
• /
• 2004

Objective: This study was performed to evaluate the possibility of prolonged culture of human embryonic stem cells (hESC; SNUhES2) on human amniotic fluid cells (hAFC), which had been storaged after karyotyping. Method: The hAFC was prepared for feeder layer in the presence of Chang's medium and STO medium (90% DMEM, 10% FBS) at $37^{circ}C$ in a 5% $CO_2$ in air atmosphere. Prior to use as a feeder layer, hAFC was mitotically inactivated by mitomycin C. The hESCs on hAFC were passaged mechanically every seven days with ES culture medium (80% DMEM/F12, 20% SR, bFGF). Results: The hAFC feeder layer support the growth of undifferentiated state of SNUhES2 for at least 59 passages thus far. SNUhES2 colonies on hAFC feeder appeared slightly angular and flatter shape as compared with circular and thicker colonies observed with STO feeder layer and showed higher level with complete undifferentiation in seven days. Like hESC cultured on STO feeders, SNUhES2 grown on hAFC expressed normal karyotype, positive for alkaline phosphatase activity, high telomerase activity, Oct-4, SSEA-3, SSEA-4, Tra-1-60 and Tra-1-81 and formed embryoid bodies (EBs). Conclusion: The hAFC supports undifferentiated growth of hESC. Therefore, these results may help to provide a clinically practicable method for expansion of hESC for cell therapies.