• 한국임상수의학회지
• /
• 제30권4호
• /
• pp.324-328
• /
• 2013

A 5-year-old intact male Yorkshire terrier dog was referred with a 1-week history of blindness. In both eyes, menace responses and pupillary light reflexes were absent. The indirect ophthalmoloscopy revealed multiple small gray-white and discoloration lesion in mid-nontapetum area in both eyes. Electroretinograms showed diminished amplitude of a- and b-waves. Multifocal serous retinal detachments were detected with optical coherence tomography (OCT) over the small gray-white and discoloration lesion in the fundus. After one week of treatments, the indirect ophthalmoloscopy showed that irregular patches and hyperpigmentation in the center of hyperreflectivity area. Reattachment and retinal thinning were observed by the OCT. The results indicate that OCT scanning is considered to be a useful method for retinal evaluation in dogs with chorioretinitis.

• Journal of the Optical Society of Korea
• /
• 제15권2호
• /
• pp.111-117
• /
• 2011

Full-range spectral domain optical coherence tomography (SD-OCT) with a 1-${\mu}m$ band light source is shown here. The phase of the reference beam is continuously stepped while the probing beam scans the sample laterally (B-scan). The two dimensional spectral interferogram obtained is processed by a Fourier transform method to obtain a complex spectrum leading to a full-range OCT image. A detailed mathematical explanation of the complex conjugate resolving method utilized is provided. The system's measurement speed was 7.96 kHz, the measured axial resolution was $9.6{\mu}m$ in air and the maximum sensitivity 99.4 dB. To demonstrate the effect of mirror image elimination, In vivo human eye pathology was measured.

• 대한화학회지
• /
• 제55권6호
• /
• pp.969-973
• /
• 2011

The synthesis, as well as spectroscopic and biological studies of a novel class of [3-(4-substitutedphenylamino)-8-azabicyclo [3.2.1] oct-8yl]-phenyl-methanone derivatives are described. All the synthesized compounds were characterized by elemental analysis FTIR, $^1H$-NMR, $^{13}C$ NMR, and Mass spectral data. All the synthesized compounds were exhibit in vitro antibacterial activity.

• Asian-Australasian Journal of Animal Sciences
• /
• 제33권12호
• /
• pp.2021-2030
• /
• 2020

Objective: Quantitative polymerase chain reaction (qPCR) has been extensively used in the field of mesenchymal stem cell (MSC) research to elucidate their characteristics and clinical potential by normalization of target genes against reference genes (RGs), which are believed to be stably expressed irrespective of various experimental conditions. However, the expression of RGs is also variable depending on the experimental conditions, which may lead to false or contradictory conclusions upon normalization. Due to the current lack of information for a clear list of stable RGs in bovine MSCs, we conducted this study to identify suitable RGs in bovine MSCs. Methods: The cycle threshold values of ten traditionally used RGs (18S ribosomal RNA [18S], beta-2-microglobulin [B2M], H2A histone family, member Z [H2A], peptidylprolyl isomerase A [PPIA], ribosomal protein 4 [RPL4], succinate dehydrogenase complex, subunit A [SDHA], beta actin [ACTB], glyceraldehyde-3-phosphate dehydrogenase [GAPDH], TATA box binding protein [TBP], and hypoxanthine phosphoribosyltrasnfrase1 [HPRT1]) in bovine bone marrow-derived MSCs (bBMMSCs) were validated for their stabilities using three types of RG evaluation algorithms (geNorm, Normfinder, and Bestkeeper). The effect of validated RGs was then verified by normalization of lineage-specific genes (fatty acid binding protein 4 [FABP4] and osteonectin [ON]) expressions during differentiations of bBMMSCs or POU class 5 homeobox 1 (OCT4) expression between bBMMSCs and dermal skins. Results: Based on the results obtained for the three most stable RGs from geNorm (TBP, RPL4, and H2A), Normfinder (TBP, RPL4, and SDHA), and Bestkeeper (TBP, RPL4, and SDHA), it was comprehensively determined that TBP and RPL4 were the most stable RGs in bBMMSCs. However, traditional RGs were suggested to be the least stable (18S) or moderately stable (GAPDH and ACTB) in bBMMSCs. Normalization of FABP4 or ON against TBP, RPL4, and 18S presented significant differences during differentiation of bBMMSCs. However, although significantly low expression of OCT4 was detected in dermal skins compared to that in bBMMSCs when TBP and RPL4 were used in normalization, normalization against 18S exhibited no significance. Conclusion: This study proposes that TBP and RPL4 were suitable as stable RGs for qPCR study in bovine MSCs.

• Clinical and Experimental Reproductive Medicine
• /
• 제28권1호
• /
• pp.33-40
• /
• 2001

Objective: This study was to establish the human embryonic stem (ES) cells derived from frozen-thawed blastocyst stage embryo that were destined to be discarded after five years in routine human IVF-ET program. Methods: Frozen-thawed and survived human blastocysts were treated by immunosurgery, and recovered ICM cells were cultured onto STO feeder cell layer and ICM colony was subcultured by mechanical dissociation into clumps. To identify ES cell, alkaline phosphatase staining and expression of Oct4 in replated ICM colonies were examined. Also, to examine the possibility of ES cell differentiation, retinoic acid (RA), basic fibroblast growth factor (b-FGF), nerve growth factor (NGF) were added in culture medium. In addition, to classify the specific cell type, differentiated cells were stained by indirect immunocytochemistry. Results: One ICM colony recovered from frozen-thawed six blastocysts was subcultured, continuously replated during 40 passage culture duration without differentiation. Subcultured colonies were strong positively stained by alkaline phophatase. When the expression of Oct4 in cultured ES colony was examined, Oct4b type is more clearly indicated than Oct4a one although there was not detected in embryoid body or differentiated cells. In differentiated cardiomyocytes from ES colony, cells were beaten regularly (60 times/min). In differentiated neural cells from ES colony, neurofilament (NF) 200 kDa protein, microtubule associated protein (MAP) 2 and ${\beta}$-tubulin of specific marker in neurons, glial fibrillary acidic protein (GFAP) of specific marker in astrocytes and galactocelebrocide (GalC) of specific marker in oligodendrocytes were confirmed by indirect immunocytochemistry. Also, muscle cells were detected by indirect immunocytochemistry. In addition, ES colonies can be successfully cryopreserved. Conclusion: This study suggested that establishment of human ES cells can be successfully derived from frozen-thawed blastocysts that were destined to be discarded, and obtained specific cell types (cardiomyocytes, neurons and muscle cells) through the in vitro differentiation procedures of ES cells.

• 한국자원식물학회지
• /
• 제11권2호
• /
• pp.136-141
• /
• 1998

To give some information on the harvest and storage of Chinese yam (Dioscorea opposita), the experiment was done to examine the effect of its harvesting date and storage method on tuber weight, tuberrelated characters during the storageor at the end of storage. The yam tubers harvested 4 times at 20 days interval from Oct. 15 were stored with different storage methods(pit, room and cold temperature) and on Mar.15. all the characters were measured. Fresh weight of the tuber harvestedafter Nov. 5 and stored were greater than that done on Oct. 15 while dry tuber weight did not have any difference between the harvesting dates. The fresh weigth done directly from the field on Mar. 15 was higher compared to the other harvesting dates owing to its higher moisture content. The rate of sound tuber stored at room or cold temperature condition was higher than that at the field at which the rate was greater at the harvest after late nov. compared to the earlier harvests. Although the two storages were similar at sound tuber rates and marketable yield, the tuber weight was more reduced when stored at room temperature than when done at cold temperature . Brightness and value 'b' of chromaticaity realted to the tuber coloring and sugar content were higher but moisture content was lowerwhen harvested on Oct. 15 than the other havesting dates. All the characteristics realted to the marketability after storage were nearly same when stored at room and cold temperature. Value 'a' of chromaticity and sugar content were higher when harvest on Mar. 15 than when harvested before Dec. 15 but the 'b' value and mositure content were reverse results.

• 한국전기전자재료학회:학술대회논문집
• /
• 한국전기전자재료학회 2000년도 하계학술대회 논문집
• /
• pp.942-945
• /
• 2000

Recently, electret applications have placed the focus on polymer thin film because electrical properties of a blend are studied. In this paper, we hale measured the infra sonic frequency by using Electret Sensor. Electret is formed by appling the voltage range of -4[kV] to -8[kV] to PTFE film and the sensor is manufactured by moment method to detect the infra-sonic signal. Electret Infrasonic Transducer, which is designed and manufactured by using of the moment method according to the potential and electric field simulation , shows its promising result, since the average rising rate of sensitivity is 7.68 [dB/oct] under 1[Hz], and the average values are within ${\pm}$ 1 [dB/oct]. As a result, it is believed that the characteristics of acquired transducer can be applied to the medical treatment, the industry, and the animal life researches, and also the study on noise elimination is required.

• Journal of Astronomy and Space Sciences
• /
• 제11권1호
• /
• pp.21-30
• /
• 1994

VV Cep-type long period spectroscopic-eclipsing binary AZ Cas has been observed for five years, 1985 Oct~1990 Feb, UBV at the Ilsan Station of Yonsei University Observatory. A total of 431 observations (U=129, B=142 and V=160) are made for 86 nights. Instrumental differential UBV and B-V light curves made with these observations cover phases nearly a half of one period. There is no appreciable light variation in V but in other two passbands a gradual decrease of the brightness is clearly noticed. The loss of light in B resulted in a reddening in $\Delta(B-V)$ by + 0.06 at phases between 0.4~0.5 as compared with that of at phase ~0.1. This intrinsic reddening arouses a question why at the orbital phase of the transit of a hot star in front of a cool M supergiant the heating of the facing hemisphere of M supergiant by the strong radiation from the B stat is absent. With regard to this unusual situation we propose a hypothesis that a large amount of gas stream of low temperature ejected from the surface of M supergiant component towards the B star dominates the brightness of B star and the reflection effect.

• Reproductive and Developmental Biology
• /
• 제32권4호
• /
• pp.223-227
• /
• 2008

Human embryonic stem (ES) cells retain the capacity for self-renewal, are pluripotent and differentiate into the three embryonic germ layer cells. The regulatory transcription factors Oct4, Nanog and Sox2 play an important role in maintaining the pluripotency of human ES cells. The aim of this research was to identify unknown genes upregulated in human ES cells along with Oct4, Nanog, and Sox2. This study characterizes an unknown gene, named chromosome 1 open reading frame 31 (C1orf31) mapping to chromosome 1q42.2. The product of C1orf31 is the hypothetical protein LOC388753 having a cytochrome c oxidase subunit VIb (COX6b) motif. In order to compare expression levels of C1orf31 in human ES cells, human embryoid body cells, vascular angiogenic progenitor cells (VAPCs), cord-blood endothelial progenitor cells (CB-EPCs) and somatic cell lines, we performed RT-PCR analysis. Interestingly, C1orf31 was highly expressed in human ES cells, cancer cell lines and SV40-immortalized cells. It has a similar expression pattern to the Oct4 gene in human ES cells and cancer cells. Also, the expression level of C1orf31 was shown to be upregulated in the S phase and early G2 phase of synchronized HeLa cells, leading us to purpose that it may be involved in the S/G2 transition process. For these reasons, we assume that C1orf31 may play a role in on differentiation of human ES cells and carcinogenesis.

• Macromolecular Research
• /
• 제17권10호
• /
• pp.739-745
• /
• 2009

N,N'-(bicyclo[2,2,2]oct-7-ene-tetracarboxylic)-bis-L-amino acids 3a-g were synthesized by the condensation reaction of bicyclo[2,2,2]oct-7-ene-2,3,5,6-tetracarboxylic dianhydride 1 with two equimolars of Lalanine 2a, L-valine 2b, L-leucine 2c, L-isoleucine 2d, L-phenyl alanine 2e, L-2-aminobutyric acid 2f and L-histidine 2g in an acetic acid solution. Seven new poly(amide-imide)s PAIs 5a-g were synthesized through the direct polycondensation reaction of seven chiral N,N'-(bicyclo[2,2,2]oct-7-ene-tetracarboxylic)-bis-L-amino acids 3a-g with bis(3-amino phenyl) phenyl phosphine oxide 4 by two different methods: direct polycondensation in a medium consisting of N-methyl-2-pyrrolidone (NMP)/triphenyl phosphite (TPP)/calcium chloride ($CaCl_2$/pyridine (py), and direct polycondensation in a tosyl chloride (TsCl)/pyridine (py)/N,N-dimethylformamide (DMF) system. The polymerization reaction produced a series of flame-retardant and thermally stable poly(amide-imide)s 5a-g with high yield. The resulted polymers were fully characterized by FTIR, $^1H$ NMR spectroscopy, elemental analyses, inherent viscosity, specific rotation and solubility tests. Data obtained by thermal analysis (TGA and DTG) revealed that the good thermal stability of these polymers. These polymers can be potentially utilized in flame retardant thermoplastic materials.