• Korean Journal of Veterinary Research
• /
• v.53 no.2
• /
• pp.109-115
• /
• 2013

Cancers are mainly sustained by a small pool of neoplastic cells, known as cancer stem cells or tumorinitiating cells. These cells possess the ability to self-renew and proliferate, and are thus able to form the tumor. In the present study cells that correspond to cancer stem cells in mammary and liver cancers in animals were identified by the expression of CD133, CD44, CK7, and OCT4 using immunochemistry. As a result, we found with CD133+ and CD44+ cancer stem cell-like phenotypes in mouse and canine hepatocellular carcinoma and canine mammary gland tumors. However, CK7+ and OCT4+ cells were not identified in animal mammary and liver cancer. CD133+ and CD44+ cells are wellknown stem cell lines and play key roles in development and metastasis in human cancer. These findings suggest that cancer stem cells are involved in animal tumorigenesis and may provide insight into mechanisms in cancer development as well as cancer diagnostics.

• Korean Journal of Crystallography
• /
• v.8 no.2
• /
• pp.132-137
• /
• 1997

The crystal structure of N,N'-di-tert-butoxycabonyl-2,7-diazabicyclo[3.3.0]oct-4-ene has been determined from single crystal x-ray diffraction study; C16H26N2O4, Triclinic, P1, a=11.119(1) Å, b=13.638(1) Å, c=6.214(1) Å, α=92.14(1)°, β=103.49(1)°, γ=73.35(1)°, V=877.4(2)Å3, T=293(2)K, Z=2, CuKα(λ=1.5418Å). The structure was solved by direct method and refined by full-matrix least squares to a final R=5.38% for 2389 unique observed F0>4σ(F0) reflections and 225 parameters.

• Plant Resources
• /
• v.7 no.3
• /
• pp.206-210
• /
• 2004

In order to find out the optimum harvest (dipping) date combined with sowing date on yield and nutrient quality of forage rye which is suitable at the Southern part of Korea, Paldanghomil variety was grown Sep. 2001 to May 2002 at Sunchon National University, and yield and nutrient quality of plant were observed. As harvest date and sowing date were delayed, the plant length was longest, number of leaves per plant was increased in the time of May 20 clipping. Fresh yield was the heaviest in the time of May 20 clipping and Oct. l0 sowing, and the most dry matter yield was the heaviest in the time of May 20 clipping and Oct. 10 sowing. Content of crude protein was the highest and that of crude fiber such as NDF, ADF, hemicellulose, cellulose and lignin were the lowest in the late time of clipping and sowing. Further more IVDMD was high and dry matter yield and digestible dry matter yield were the highest in the time of May 20 dipping and Oct. 10 sowing. Judging from the results reported above, at optimum harvest (dipping) date combined with sowing date for yield and nutrient quality of forage rye seemed to be the time of May 20 clipping and Oct. 10 sowing.

• Journal of the Optical Society of Korea
• /
• v.15 no.1
• /
• pp.74-77
• /
• 2011

We report a novel extension of 840 nm wavelength- based spectral domain optical tomography to in vivo/real-time human middle ear diagnosis. The system was designed to access the middle ear region with a specifically dedicated handheld probe. The real-time displaying feature was mandatory for in vivo imaging human subject with the handheld probe, and the system could provide about 20 frames per second for 2048 pixels by 1000 A-scans without using any graphics process units under the Labview platform. The inner ear structure of a healthy male volunteer was imaged with the developed system with the axial and lateral resolutions of $15\;{\mu}m$ and $30\;{\mu}m$, respectively. The application of the OCT technology to early diagnose otitis media(OM) is very promising and could be another extensive branch in the OCT field because it provides the depth resolved image including tympanic membrane (TM) and structures below TM whereas the conventional otoscope technique only gives asurface image of the TM.

• Proceedings of the Korea Information Processing Society Conference
• /
• 2022.11a
• /
• pp.541-543
• /
• 2022

In recent years, retinal disorders have become a serious health concern. Retinal disorders develop slowly and without obvious signs. To avoid vision deterioration, early detection and treatment are critical. Optical coherence tomography (OCT) is a non-invasive and non-contact medical imaging technique used to acquire informative and high-resolution image of retinal area and underlying layers. Disease signs are difficult to detect because OCT images have many areas which are not related to any disease. In this paper, we present a deep learning-based method to perform multi-label classification on a long-tailed OCT dataset. Our method first extracts the region of interest and then performs the classification task. We achieve 98% accuracy, 92% sensitivity, and 99% specificity on our private OCT dataset. Using the heatmap generated from trained convolutional neural network, our method is more robust and explainable than previous approaches because it focuses on areas that contain disease signs.

• The Journal of the Korea Contents Association
• /
• v.12 no.8
• /
• pp.240-247
• /
• 2012

Marginal and internal fit is an important part of the longevity of dental restorations. The purpose of this study was to analysis the marginal and internal fit of zirconia core by dental CAD/CAM system using innovative and non-destructive methods such as optical coherence tomography(OCT) and compare with conventional method such as silicone replica technique(SRT). Ten dental stone models of abutment of maxillary right central incisal were manufactured and scanned. Ten zirconia cores were fabricated with commercial CAD/CAM system. To measure the marginal and internal fit of each sample, five point of fitness were measured using 2 different methods(OCT and SRT). Statistical analysis was performed using independent sample t-test(${\alpha}$=0.05). OCT and SRT groups were not significantly different(P>0.05). By this results, analysis the fitness of dental restorations using OCT were acceptable measuring method.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.15 no.4
• /
• pp.238-244
• /
• 1995

The objective of this study is to obtain the basic data for investigating the effects of organic reserves on winter survial or regrowth yield. Forage rape(Brassica napus L.) was sown on Sep. 1, 1994. Experimental plots were divided into three replicates under 6 different cutting dates(l0 days interval from Oct. 15 to Dec. 4). Field-grown palnts were sampled on the each cutting date and on the wintering period (Jan. 16) to analyze the nitrogen and non-structural cahohydrate reserves. The rate of winter survival and regrowth yield were also measured in the spring of next year. On the before wintering, dry matter yields were 152, 274, 500, 718, 776 and 981 kg/lOa, respectively, from the cutting date on Oct. 15, Oct. 25, Nov. 4, Nov. 14, Nov. 24, and Dec. 4. Cmde protein yield significantly increased as cutting date was later until Nov. 14, thereafter a significant increase did not occured. Nitrogen and starch contents per plant significnatly increased as the cutting date was later. The increasing rate of starch was greatly higher than that of nitrogen. On the wintering period, nitrogen reserves in mts were 85.3, 68.8, 47.6, 28.3, 44.3, and 55.3 mglplant, and starch reserve were 11 1.3, 75.3, 39.3, 19.6, 26.4 and 34.6 mglplant, respectively, in the plots cut on Oct. 15, Oct. 25, Nov. 4, Nov. 14, Nov. 24, and Dec. 4. It showed that carbohydrate reserves were much highly utilized than nitrogen reserves during wintering period. The rates of winter survival were 91, 83, 46, 22, 35 and 43% and regrowth yields were 692, 545, 316, 84, 127 and 140 kgD.M/lOa, respectively, in each plots. The highly significant correlation (p<0.01) between the level of organic reserves and the rate of winter survival or regrowth yield were obtained.

• Microbiology and Biotechnology Letters
• /
• v.19 no.1
• /
• pp.82-87
• /
• 1991

Xanthomonns curnpestris M12, Xunthornonas sp. M28, Acinetuhucter Iwofz GI, and Klebsiella pneumoniae L25, Pseudomonas rnaltophiliu N246 were screened to increase the ability for crude oil utilization. All of these could utilize hexadecane and octane with the exception of N246 strain for only octane biodegradation. Thus N246, M12, and M28, strains were specially examined for octane oxidation. Octane biodegradation by three strains showed the optimal conditions at $30^{\circ}C$, pH 7.0~9.0, and 0.2~0.3% octane concentration as a substrate. It was found that P. multofihila N246 and X. curnpestns M12 had plasmid and the cured plasmid from N246 strain lost octane uitilization. Therefore, it was confirmed that certain genes for octane utilization were Iocated on OCT plasmid in N246 strain. The size of OCT plasmid in N246 strain was 118 kb. The N246 strain was resistant to ampicillin.

• Journal of the Korean Society of Safety
• /
• v.31 no.1
• /
• pp.13-18
• /
• 2016

KTX wheelset bearings have thus far been maintained in accordance with the maintenance system of French national railway company, SNCF. The overhaul cycle time (OCT) of KTX wheelset bearings is now 1.4 million km in mileage. This value, however, has not been validated in Korea that has much different railway environments with France. In fact, it is impossible to validate OCT of wheelset bearings directly because they are disassembled and cleaned only when some faults are detected. In this paper, the accuracy of the current OCT value was evaluated indirectly by investigating the effect of grease lubricants on the bearing life. Five grease samples (one new and four aged greases) were used in this study. Four aged greases of different conditions were obtained from four wheelset bearings whose mileages were about 0.3, 1.2, 1.3, and 1.8 million km, respectively. Each grease sample was then injected into the RCF (Rolling Contact Fatigue) tester and fatigue lifetime was experimentally estimated. In addition, the wt% of Fe in each grease sample was analysed. The experiment results reveal that the bearing lifetime is inversely proportional to the mileage of grease sample while the wt% of Fe increases with the mileage of grease sample. Based on the experimental results, it can be concluded that the current OCT value is appropriate for the first overhaul of wheelset bearings. However, further validation is required to determine the second and third OCT values.

• BMB Reports
• /
• v.44 no.12
• /
• pp.793-798
• /
• 2011

Recently, pluripotency induction or cellular reprogramming by introducing critical transcription factors has been extensively studied, but has been demonstrated only in vitro. Based on reports that Oct4 is critically involved in transforming neural stem cells into pluripotent cells, we used the lentiviral vector to introduce the Oct4 gene into the hippocampal dentate gyrus (DG) of adult mice. We examined whether this manipulation led to cellular or behavioral changes, possibly through processes involving the transformation of NS cells into pluripotent cells. The Oct4 lentivirus-infused group and the green fluorescent protein lentivirus-infused group showed a similar thickness of the DG and a comparable level of synaptophysin expression in the DG. Furthermore, our behavioral analyses did not show any differences between the groups concerning exploratory activity, anxiety, or memory abilities. This first trial for pluripotency induction in vivo, despite negative results, provides implications and information for future studies on in vivo cellular reprogramming.