• Plant Biotechnology Reports
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• v.4 no.2
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• pp.125-128
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• 2010

Culture conditions were established for high frequency plant regeneration via somatic embryogenesis from cell suspension cultures of Nymphoides coreana. Zygotic embryos formed pale-yellow globular structures and calluses at a frequency of 85.6% when cultured on half-strength Murashige and Skoog (MS) medium supplemented with 0.3 $mg\;l^{-1}$ of 2,4-D. However, the frequency of pale-yellow globular structures and white callus formation decreased slightly with an increasing concentration of 2,4-D up to 10 $mg\;l^{-1}$ with the frequency rate falling to 16.7%. Cell suspension cultures were established from zygotic embryo-derived calluses using half-strength MS medium supplemented with 0.3 $mg\;l^{-1}$ of 2,4-D. Upon plating onto half-strength MS basal medium, over 92.3% of cell aggregates gave rise to numerous somatic embryos and developed into plantlets. Regenerated plantlets were successfully transplanted into potting soil and achieved full growth to an adult plant in a growth chamber. The high frequency plant regeneration system for Nymphoides coreana established in this study will be useful for genetic manipulation and cryopreservation of this species.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.6 no.1
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• pp.41-50
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• 2003

The feasibility as floricultural crops and water garden plant materials of Korean native water plants was investigated. Propagation type, growing and flowering time were observed for development as water garden, interior aquarium plant and water purification materials. Flowering time of the water plant was 7 species in May, 28 species in June, 49 species in July, 55 species in August, 47 species in September, and 17 species in October. Beautiful flowering water plants were Nymphaeaceae, Nymphoides peltata, Nymphoides indica, Monochoria korsakowii, Iris pseudacorus, Iris laevigata, and etc. Ornamental leafy water plants were Ceratopteris thalictroides, Ludwigia ovalis, Myriophyllum verticillatim, Limnophila sessiliflora, Blyxa aubertii, Blyxa echinosperma, Vallisneria asiatica, Hydrilla verticillata and Eleocharis acicularis etc. Isoetes japonica, Isoetes coreana and Isoetes sinensis were propagated by spore. Blyxa aubertii, Blyxa echinosperma, Myriophyllum verticillatim, Nuphar japonicum, Nelumbo nucifera, Ottelia alismoides, Sagittaria aginashi, Trapa japonica, and Trapa natans were propagated by seed. Persicaria amphibia, Ceratophyllum demersum (hornwort), Myriophyllum verticillatim, Myriophyllum spicatum, Oenanthe javanica, Potamogeton crispus, Hydrilla verticillata and Acorus calamus were propagated by division. And Vallisneria asiatica, Hydrilla verticillata and Phragmites japonica were propagated by runner. Ceratophyllum demersum (hornwort), Myriophyllum verticillatim, Myriophyllum spicatum, Limnophila sessilifera were propagated by adventitious bud. Ceratopteris thalictroides was propagated by leaf cutting. The 35 genera, 68 species of water plants were available for horticultural use. The 45 species such as Iris laevigata, Eleocharis acicularis, Menyanthes trifoliata, Nymphaea minima, Nuphar pumilum, Nymphoides coreana, Nymphoides peltata, Nymphoides indica, Nymphaea tetragona (water lily), and Typha latifolia could be use for water garden plant. The 21 species such as Limnophila sessilifera, Vallisneria asiatica, Ceratophyllum demersum and Hydrilla verticillata available for indoor aquarium. The 19 species such as Ottelia alismoides, Oenanthe javanica, Limnophila sessilifera and Blyxa echinosperma could be culture in container. The 27 species such as Trapa japonica, Trapa incisa, Phramites commuris (reed), Phragmites japonica, and Zizania latifolia were usable for water purification plant materials.

• Journal of Wetlands Research
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• v.11 no.2
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• pp.17-28
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• 2009

The investigated Varcular plants in Youngsan River Streams of Downtown in Gwangju Metropolitan City consist of total 437 taxa: 2 forms, 49 varieties, 386 species, 265 genera, 91 families. Among 53 taxa of hydrophytes, emerged plants were 16taxa, floating-leaved plants were 10taxa, suvmerged plants were 10taxa, and free-floating plants were 3taxa and swamp plants were 14taxa. Based on the list of Rare and Endangered plants, 5taxa were recorded such as Hydrocharis dubia, Euryale Ferox, Penthorum chinense, Prunus yedoensis, Nymphoides coreana. And Korean endemic Plants were appeared as 5taxa: Poa annua, Forsythia koreana, Paulownia coreana, Galium koreanum, Aster koraiensis. From the specific plant species sorted by classes, class I has 10taxa, class II has 2taxa, class III has 2taxa, class IV has 3taxa, class V has 5taxa. Naturalized plant were listed as 62taxa: 15families, 44genera, 59species, 3varieties, and naturalization index was 14.19%. The ecosystem disturbance plants assigned by the Ministry of Environment, 3taxa were recorded: Paspalum distichum, Ambrosia artemisiifolia var. elatior, Solanum carilinense.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.9 no.4
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• pp.24-35
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• 2006

The vascular plants of the studied area was listed 384 taxa of 90 families, 234 genera, 331 species, 1 subspecies, 45 varieties and 7 forms. The flora of each reservoir, 285 taxa of 83 families, 190 genera, 247 species, 1 subspecies, 30 varieties, 7 forms in Osongjae, 117 taxa of 39 families, 87 genera, 100 species, 17 varieties in Ogongjae, 83 taxa of 30 families, 67 genera, 73 species, 8 varieties, 2 forms in Shinyongjae and 86 taxa of 33families, 70 genera, 75 species, 1 subspecies, 10 varieties in Eodujae. Based on the list of the rare plants by the Forest Research Institute, 3 taxa were recorded in the studied areas; Magnolia kobus (Preservation priority order : No. 187), Penthorum chinense (No. 144), Prunus yedoensis (No. 110) and based on the list of Korean endemic plants, 4 taxa were recorded; Populus tomentiglandulosa, Clematis trichotoma, Prunus yedoensis, Paulownia coreana. Specific plant species by floral region were total 15 taxa; Prunus yedoensis in class V, 2 taxa (Carex idzuroei, Magnolia kobus) in class IV, 2 taxa (Monochoria korsakowi, Poncirus trifoliata ) in class III, 10 taxa (Sagittaria aginashi, Salix glandulosa, Clematis brachyura, Nymphoides peltata, etc.) in class I. The naturalized plants in this site were 13 families, 29 genera, 43 species, 1 varieties, 44 taxa and naturalization rate was 11.5% and based on the list of an ecosystem disturbance plants, 3 taxa were recorded in the studied areas : Paspalum distichum, Solanum carolinense, Ambrosia artemisiifolia var. elatior. The hydrophytes was listed 31 taxa of 15 families, 20 genera, 29 species, 2 varieties and Eleocharis mamillata var. cyclocarpa, Penthorum chinense, Soirodela polyrhiza were recorded in Osongjae, Sagittaria aginashi, Eleocharis kuroguwai, Scirpus juncoides, Scirpus triqueter, Monochoria korsakowi, Monochoria vaginalis var. plantaginea were recorded in Ogongjae. Besides, Scirpus fluviatilis, Trapa pseudoincisa were recorded in Shinyongjae and Scirpus triangulatus, Nymphoides peltata, Myriophyllum verticillatum were recorded in Eodujae. This site is located in the urban area and ecosystem-disturbing wild animal and plant has been imported here, therefore ecosystem has been disturbed more and more. The wetland here rapidly has changed into upland, so it needs conservation measures through long-term monitoring.

• Korean Journal of Ecology and Environment
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• v.51 no.2
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• pp.174-183
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• 2018

In the Sumeunmulbaengdui Wetland Protected Area of Jeju Island, there exists a unique pond, a so-called 'Ohmi', which is likely to be an oasis supporting the wildlife and escaped-livestock. We exquisitely described the diversity and distribution of plant community of the Ohmi. Despite its small area, six syntaxa were identified: Sphagnum community of peat bog type, Nymphoides coreana community of the floating-leaf limnetic vegetation, Scirpus triangulatus community and Schoenoplectus hotarui community of the littoral zone vegetation, and Deinostema violacea-Eleocharis congesta community and Eriocaulon atrum-miquelianum community of the ephemeral vegetation. A zonal distribution of plant communities along the water depth was recognized. The Sphagnum islets regarded as a typical component of peat bog, in Korea were first described at the center of the Ohmi. We conclude that the Ohmi is a primeval keystone habitat of the Sumeunmulbaengdui intermediate moor with a less oligotrophic ombrotrophic environment.

• Journal of Environmental Science International
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• v.18 no.4
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• pp.411-422
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• 2009

This study was investigated on biodiversity and life forms of plants distributed in 28 wetlands on Jeju city, in order to apply wetlands to nature exploration sites for environmental education. The 131 kinds of the plants were found on 28 wetlands. The plant biodiversity was relatively high in Ban pond, Jeongmool, Mosanimool, Dolgaegi pond, Woot pond, and etc. The $5{\sim}6$ life forms of plants, relatively high in variousness to other wet lands, were distributed in Mosanimool, Dolgaegi pond, Woot pond, and Yeonhoa pond as well as Jeongmool and Yongsoo reservoir. The 71 kinds, which correspond to 54.2% of plants observed in this study, were identified as hydrophytes. The $4{\sim}5$ forms of hydrophytes were distributed on 23 wetlands, including Jeongmool, Mosanimool, Yongsoo reservoir, Ban pond, Dolgaegi pond, and etc. In conclusion, 5 wetlands such as Jeongmool, Yongsoo reservoir, Dolgaegi pond, Mosanimool and Woot pond were high in variousness of plant life forms as well as biodiversity of plants. These results suggest that 5 wetlands above could be applied as nature exploration sites. In addition, Korean rare and endangered plants such as Isoetes japonica, Nymphoides coreana, Utricularia japonica and Sparganium stoloniferum in this study need to be conserved.

• Korean Journal of Plant Taxonomy
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• v.41 no.4
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• pp.429-439
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• 2011

We investigated the flora of Imja-do(Isl.), an island of the Shinan archipelago, located in Imja-myeon, Shinan-gun, Jollanamo-do and discussed the vascular plants found there. Twenty-four separate field trips (100days) were completed from August of 1997 to October of 2009. As a result, we identified the distribution of 526 taxa in Imja-do(Isl.), comprising 112 families, 331 genera, 463 species, 1 subspecies, 60 varieties, and 2 forms. There are a total of 78 taxa of floristic regional indicator plants and one taxon of level II endangered species, Millettia japonica, as designated by the Korean Ministry of the Environment. The floristic regional indicator plants are as follows; four taxa of level V such as Utricularia bifida, two taxa of level IV such as Cladium chinense, 14 taxa of level III such as Aletris spicata, three taxa of level II, and 55 taxa of level I. In addition, one endangered species (EN), Nymphoides coreana, five vulnerable species (VU),such as Ilex cornuta, and three near-threatened species (NT) such as Utricula riaracemosa, were found in the investigated area. The plants of Imja-do(Isl.) are classified into four types based on the plant geographical origin, as follows: 1) boreal plants advancing southward in a glacial period, 2) temperate plants spreading eastward from the Himalayas and China to Japan passing through the south coast of Korea, 3) subtropical plants, and 4) indigenously developed endemic species of the warm-temperate region of East Asia.