The Journal of the Convergence on Culture Technology
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v.6
no.3
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pp.457-462
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2020
Osteoporosis is a disease that occurs mainly in the elderly and increases the risk of fractures due to structural deterioration of bone mass and tissues. The purpose of this study are to assess the relationship between nutritional components and osteoporosis and to evaluate models for predicting osteoporosis based on nutrient components. In experimental method, association was performed using binary logistic regression, and predictive models were generated using the naive Bayes algorithm and variable subset selection methods. The analysis results for single variables indicated that food intake and vitamin B2 showed the highest value of the area under the receiver operating characteristic curve (AUC) for predicting osteoporosis in men. In women, monounsaturated fatty acids showed the highest AUC value. In prediction model of female osteoporosis, the models generated by the correlation based feature subset and wrapper based variable subset methods showed an AUC value of 0.662. In men, the model by the full variable obtained an AUC of 0.626, and in other male models, the predictive performance was very low in sensitivity and 1-specificity. The results of these studies are expected to be used as the basic information for the treatment and prevention of osteoporosis.
This experiment was carried out to investigate the bacteriolytic action of the egg white lysozyme isolated from Korean native Ogol fowl and to obtain the data for utilization of the enzyme as a food preservative. Staphyococcus aureus phage type 29 and Bacillus subtilis ATCC 6633 among the microorganisms tested were lyzed by the treatment with 0.05% lysozyme, but Staphylococcus aureus phage type 57 in addition to E. coli etc. was found to be a lysozyme- insensitive species. The lysis of S. aureus phage type 29 was maximized when incubated in nutrient broth (pH 7.0) at $37^{\circ}C$ for 24 hours and suspended it to absorbance 0.6 at 540nm in 0.05M sodium acetate but fer (pH 4.5) and then treated it with the 0.05% lysozyme for 30 min. at $30^{\circ}C$. It was found that the effect of 0.05% lysozyme in combination with 1% glycine on the growth inhibition of S. aurecus phage type 29 increased more 50% than that in the absence of glycine, but not effect with other any additeves and metal ions tested.
This study was conducted in to elucidate the changes of growth characteristics and photosynthesis in three soybean (Glycine max L. cv. Danwonkong, Hwangkeumkong and Kwangankong) 30 day old seedlings to 100mM NaCl concentration containing 1/2 Hoagland's nutrient solution in sand culture. The main stem height and number of main stem node were decreased. thus, leaf area and dry matter were decreased with 100mM NaCl. Growth reduction was less little in Hwangkeumkong than other cultivars. The stem growth rate was affected less than other parts as root or leaf, by NaCl treatment. The specific leaf area (SLA), shoot : root ratio and leaf : root ratio decreased with NaCl solution except for those of Hwangkeumkong. There is no general tendency in leaf thickness by leaf position of three cultivars. The chlorophyll content (SPAD) of the primary and 2nd leaf slightly decreased under NaCl solution but rapidly increased in non-NaCl solution at 15 days after treatments. The photosynthesis, stomatal conductance and transpiration of 2nd leaf positions reduced by NaCl and there were a sigificant correlation between photosynthesis and stomatal conductance or transpiration.
Little information is available on the effects of low pH on nodulation and growth of subterranean clover cultivars. Plants of 11 cultivars were inoculated with an acid-tolerant strain of Rhizobium trifolii and grown for 28 days in continuously flowing nitrogen-free nutrient solutions maintained at six constant pH values from 4.0 to 6.5. At pH 4.0, 4 cultivars failed to nodulate and the remainder formed only a few nodules; nodulation was delayed by 2 to 3 days in this treatment. All cultivars nodulated at pH 4.5 but, with the exception of cv. Dwalganup, nodule numbers were significantly less than at $pH{\geq}5.0$. Only in cvv. Clare and Woogenellup were nodule numbers significantly greater at pH 6.5 than at 5.0. Whole plant dry matter yields were strongly depressed at pH 4.0(21 to 33% of maximum). At pH 4.5, relative yields of all cultivars except Dwalganup were significantly depressed without significant reductions in nodule dry weights, suggesting that nodule function was inhibited by acidity. These results are consistent with the view that nodulation and growth of plants dependent on symbiotic nitrogen fixation are much more sensitive to low pH per se than is host plant growth in the presence of adequate mineral nitrogen.
This study was performed to investigate the effects of CMS (Condensed Molasses Soluble, a by-product generated from molasses during the lysine production) on the nutrient uptake, growth and yield of rice. The nitrogen uptake of rice was found to be higher in CMS treatments compared to the control treatment during the growth period. The leaf color index, height of the plants and number of tillers were increased by the application of CMS. Rice yield was highest in ($7,070kg\;ha^{-1}$) and an increase of 21% was observed in 0.3% CMS treated plots compared untreated control ($3,000kg\;ha^{-1}$). The protein content of unpolished rice increased from 4 to 28% due to the effect of CMS, while Toyo palatability score decreased. Though a decrease in pH and an increase in EC values were observed with CMS application, this phenomenon did not affect the growth and yield of rice in paddy field.
A study was carried out to investigate the nutritive value and utilization of hybrid sorghum and perennial grass species viz. setaria (Setaria anceps) and hybrid napier when intercropped with soybean by growing Jersey crossbred heifers. Fifteen growing crossbred heifers (Jersey${\times}$Red Sindhi) of between 7-10 months age and pre-trial average body weight of 49-50 kg were divided on the basis of weight in to three treatment groups viz. $T_1$-hybrid sorghum+soybean, $T_2$-setaria+soybean and $T_3$-hybrid napier+soybean in a completely randomized block design. Intercropped forages were harvested fresh, chaffed and mixed before they were offered to the heifers. Chemical composition of the herbage, dry matter intake (DMI), body weight gain and nutrient digestibility co-efficients were estimated. The herbage mixtures had crude protein (CP) content in the range of 11.87 to 13.86% and ether extract (EE) contents were 2.91 to 3.11%, respectively. The herbage mixtures were rich in minerals (ash). The gross energy (kcal/g DM) was higher in hybrid napier+soybean, while hybrid sorghum+soybean and setaria+soybean herbage mixtures had lower value for gross energy. The hybrid sorghum+soybean and setaria+soybean herbage mixtures had higher contents of NDF, ADF, cellulose, lignin and silica as compared to that of hybrid napier+soybean herbage mixture. The heifers fed hybrid napier+soybean herbage mixture had significantly (p<0.05) higher $DMI\;g/kg\;W^{0.75}$ ($97.41{\pm}4.34$) as compared to hybrid sorghum+soybean ($88.31{\pm}2.66$) and setaria+soybean ($79.29{\pm}1.06$) herbage mixtures. Nutrients digestibility, DCP percent, DCP intake and nitrogen balance were significantly (p<0.05) higher in the heifers fed on hybrid napier+soybean herbage mixture. There was a significant (p<0.05) difference among different herbage mixtures in TDN. The heifers on setaria+soybean herbage mixture had lower average body weight gain (g/day) than those on hybrid sorghum+soybean and hybrid napier+soybean herbage mixtures. Data obtained in this experiment demonstrated that herbage mixture of hybrid napier+soybean was better than hybrid sorghum+soybean and setaria+soybean herbage mixtures in the nutrition of growing heifers. It had highest nutritive value, better digestibility co-efficients which showed better growth rate and higher feed efficiency. In ranking, hybrid napier+soybean herbage mixture was better followed by hybrid sorghum+soybean and setaria+soybean in nutritive value in the parameters studied. For future wasteland development program in humid-sub tropics of Himachal Pradesh hybrid napier and its intercropping with soybean is recommended for general adoption because of its better adaptability and higher nutritive value.
O'Neill, H.V. Masey;Liu, N.;Wang, J.P.;Diallo, A.;Hill, S.
Asian-Australasian Journal of Animal Sciences
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v.25
no.4
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pp.515-523
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2012
The objective of this study was to investigate the variability in broiler performance, apparent metabolisable energy (AME) and ileal digestible energy (IDE) between five different maize samples fed with and without xylanase at 16,000 U/kg. Various in vitro characterisations were conducted to determine if any could predict performance or AME. Samples of the maize were harvested in five diverse regions and fed individually in a mash diet as follows (g/kg): test maize 608.3; soya bean meal (SBM) 324.1; poultry fat 25.2; salt 4.6; met 2.6; lys 1.6; thr 0.5; limestone 9.7, dical 18.4; vit/min 5.0; CP 210 and ME (kcal/kg) 3,085. The diets were fed to 720 broilers with 6 replicates, each containing 12 birds per treatment, from 0 to 18 d of age. Maize samples were analysed for starch, protein, crude fibre, fat, protein solubility index (PSI) and vitreousness using near infra red reflectance spectroscopy (NIR). They were also assayed using an in vitro starch digestibility method. The results showed that there was no effect of harvest region on the feed intake (FI), body weight gain (BWG) or feed conversion ratio (FCR) of the broilers over the 18 d period (p = 0.959, 0.926, 0.819 respectively). There was an improvement in all parameters with the addition of xylanase (FI p = 0.011; BWG and FCR p<0.001). There was a significant positive effect of xylanase on IDE, AME, IDE Intake (IDEI) and AME intake (AMEI) (p<0.0001 in all cases). Although there was no significant effect of maize source, there was a strong trend towards variability in IDE (p = 0.066) and AME (p = 0.058). There were no significant correlations (p<0.05) between any proximate or physiochemical values and any performance or AME values. This may suggest that none of those selected were suitable predictors for performance or AME. The broilers performed well according to the breed guidelines, with slightly increased FI, increased BWG and similar FCR prior to the addition of xylanase. When FCR and BWG were analysed with FI as a covariate, xylanase addition remained significant suggesting that the improvement in BWG and FCR was driven by an increase in digestibility and nutrient availability.
Lv, J.N.;Chen, Y.Q.;Guo, X.J.;Piao, X.S.;Cao, Y.H.;Dong, B.
Asian-Australasian Journal of Animal Sciences
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v.26
no.4
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pp.579-587
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2013
A total of 288 crossbred (Duroc${\times}$Landrace${\times}$Yorkshire) growing pigs were used in two experiments to investigate the effects of adding ${\beta}$-mannanase to corn-soybean meal-based diets on pig performance and apparent total tract digestibility (ATTD). Both experiments lasted 28 d and were split into two phases namely 1 to 14 days (phase 1) and 15 to 28 days (phase 2). In Exp. 1,144 pigs weighing $23.60{\pm}1.59$ kg BW were assigned to one of four corn-soybean meal-based diets containing 0, 200, 400 or 600 U/kg ${\beta}$-mannanase. Increasing the level of ${\beta}$-mannanase increased weight gain (quadratic effect; p<0.01) and feed efficiency (linear and quadratic effect; p<0.01) during the second phase and the overall experiment. However, performance was unaffected (p>0.05) by treatment during phase 1. Increasing the amount of ${\beta}$-mannanase in the diet improved (linear and quadratic effect; p<0.05) the ATTD of CP, NDF, ADF, calcium, and phosphorus during both phases. Based on the results of Exp. 1, the optimal supplementation level was determined to be 400 U/kg and this was the level that was applied in Exp. 2. In Exp. 2, 144 pigs weighing $23.50{\pm}1.86$ kg BW were fed diets containing 0 or 400 U/kg of ${\beta}$-mannanase and 3,250 or 3,400 kcal/kg digestible energy (DE) in a $2{\times}2$ factorial design. ${\beta}$-Mannanase supplementation increased (p<0.01) weight gain and feed efficiency while the higher energy content increased (p<0.01) feed intake and feed efficiency during both phases and overall. Increased energy content and ${\beta}$-mannanase supplementation both increased (p<0.05) the ATTD of DM, CP, NDF, ADF, phosphorus, and GE during both phases. There were no significant interactions between energy level and ${\beta}$-mannanase for any performance or digestibility parameter. In conclusion, the ${\beta}$-mannanase used in the present experiment improved the performance of growing pigs fed diets based on corn and soybean. The mechanism through which the improvements were obtained appears to be related to improvements in ATTD.
Gang, Jae Sin;Gang, Seong Ho;Lee, Yun Ho;Sim, Jeong Hui;Lee, Sang Hun
ALGAE
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v.18
no.1
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pp.13-20
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2003
Two isolated Antarctic marine diatoms, Chaetoceros neogracile VanLandingham and Stellarima microtrias (Ehrenberg) Hasle and Sims were examined to show changes of growth and uptake rate of nitrate due to UV-B irradiance. Chlorophyll (chl) a concentration was regarded as the growth index of diatom. The diatoms were treated with UV-B radiation and cultured for 4 days under cool-white fluorescent light without UV-B radiation. Two levels of UV-B exposures were applies: 1 and 6 W $m^{-2}$. Durations of UV-B treatment were 20, 40 and 60 minutes under 6 W $m^{-2}$ and 1, 2, 3, 4 and 5 hrs under 1 W $m^{-2}$. The control groups were cultured at the same time without UV-B radiation. The growth rates of two diatoms decreased under 1 and 6 W $m^{-2}$ UV-B irradiances than that of control group. After 4 days, chl a concentrations of C. neogracile were increased more than 4 times from 133 μgo$l^{-1}$ to 632 μgo$l^{-1}$ in control group. However, the concentration of experimental groups under 1 W $m^{-2}$ UV-B were only increased from 139 μgo$l^{-1}$ to 421 μgo$l^{-1}$ during one hour and the chl a concentrations were decreased from 144 μgo$l^{-1}$ to 108 μgo$l^{-1}$ during five hour. Growth of diatom dramatically more decreased under 6 W $m^{-2}$ UV-B than 1 W $m^{-2}$ UV-B. The chl a concentration of experimental groups under 6 W $m^{-2}$ UV-B for one hour was only increased from 111 μgo$l^{-1}$ to 122 μgo$l^{-1}$. In the case of S. microtrias showed also similar pattern to C. neogracile by UV-B radiation. The uptake rates of nitrate by the two strains were decreased abruptly under 6 W $m^{-2}$ UV-B irradiances. When two strains were treated under 1 and 6 W $m^{-2}$ UV-B during one hour, the strains were only continued growth and uptake of nitrate under 1 W $m^{-2}$ UV-B. This experimental evidence shows that exposure to UV-B radiation especially to high irradiance of UV-B decreases diatom survival and causes lower decrease of nutrient concentrations by microalgae in Antarctic water. Furthermore, evidence suggests that microalgal communities confined to near-surface waters in Antarctica will be harmed by increased UV-B radiation, thereby altering the dynamics of Antarctic marine ecosystems.
A study was carried out to investigate the nutritive value and utilization of three grass species, batiki grass (lschaemum aristatum var. indicum), guinea grass (Panicum maximum) and signal grass (Bracharia decumbens) by growing goats. Eighteen growing crossbred goats $(Anglo-Nubian{\times}Fiji\;local)$ of between 9-11 months of age and pre-trial average live weight of $9.50{\pm}1.60kg$ were divided on the basis of weight to three treatment groups in a completely randomized design. The grasses constituted the diets and they were harvested fresh and chopped into pieces before they were offered to the goats. Chemical composition of the grasses, DMI, body weight gain (BWG) and apparent nutrient digestibility coefficients were measured. The grasses had similar DM content. The CP content of the grasses was in the range of 8.3-11.2%. Crude fiber (CF) content was between 30.9-35.2%. Ether extract (EE) was low with a range of 1.2-1.8%. Nitrogen free extract (NFE) was similar (40.9%) for batiki and guinea grasses, while signal grass had more NFE content (51.1%). The grasses are good sources of minerals (ash). OM content was higher in signal grass while guinea and batiki grasses had similar OM content. The goats on signal grass had higher DMI than those on batiki and guinea grasses (p<0.05). The goats on batiki grass had lower average BWG (p<0.05) than those on guinea and signal grasses. Nutrients digestibility were significantly (p<0.05) higher in the goats on signal grass compared to those on guinea and batiki grasses. The goats on guinea grass were better (p<0.05) in the digestibility of CP, OM, NFE and ME than those on batiki grass. However, goats on batiki were significantly better (p<0.05) in digestibility of CF than those on guinea grass. Signal and guinea grasses had more DCP than batiki grass. DE was lower in batiki grass (p<0.05) than in guinea and signal grasses. There was no significant difference (p>0.05) between batiki and guinea grasses in TON. Data obtained in this experiment demonstrated that signal grass is better than guinea and signal in the nutrition of growing goats in the tropical environment of Samoa. It had the highest nutritive value, better apparent digestibility coefficients which have better growth rate and feed efficiency. In ranking, signal grass was better than guinea and batiki grasses, while guinea grass was better than batiki in nutritive value in the parameters measured. For future pasture establishment in Samoa, signal grass is recommended for consideration because of its higher nutritive value as a replacement for batiki, the most predominant grass.
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