• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.4
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• pp.556-561
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• 1997

Vibrio cholerae non-O1 FM-3 was isolated from sea water, and it showed the same bacteriological characteristics as Vibrio cholerae non-O1 ATCC 25872. V. cholerae non-O1 FM-3 presented the highest hemolytic activity at stationary phase of its growth. The hemolytic activity was decreased in accordance with increasing of pretense activity of its cultural supernatant. The characteristics of the hemolysin produced by V. cholerae non-O1 FM-3 were investigated after partial purification with a Sephadex G-100 chromatography. The hemolytic activity of purified protein was stable at $4^{\circ}C$ while it was completely lost by heating at $60^{\circ}C$ for 30 min. The activity of hemolysin was increased by addition of divalent cations such as $Ca^{2+},\;Mg^{2+},\;and\;Mn^{2+}$ while it was inhibited by additions of $Zn^{2+}$. When the hemolysin was incubated with suspensions of erythrocytes at $4^{\circ}C$ and $37^{\circ}C$, respectively, hemolysis was not observed at $4^{\circ}C$ but at $37^{\circ}C$. It means that hemolysis by purified hemolysin was temperature-dependent while its binding step of hemolysin to cell membrane was temperature-independent.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.2
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• pp.345-352
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• 2020

This study compared how falls can be reduced in disabled elderly people by using the Otago exercise program and ascertained the program's effects on physical function and psychological recovery. The subjects were 30 people, and were assigned to two groups. The experimental group had 17 people, and the other was the control group that had 13 people. The experimental group attended the 50-minute program twice a week for 12 weeks. The outcome measures for lower extremity muscle strength, postural balance, flexibility, and self-efficacy were tested at the pre-test and post-test phases. In the analysis, general characteristics were analyzed by descriptive statistics. Physical function and self-efficacy were analyzed by independent T-test between the two groups, and correspondence T-tests were used within the two groups. The result of this study demonstrated that the experimental group showed significant increases in lower extremity muscle strength, static balance, flexibility and dynamic balance, and self-efficacy showed a small increase in the experimental group. But the control group showed significant decreases in static balance, flexibility and fall efficacy. The control group also showed decreases in lower extremity strength and dynamic balance. So the results of this study proved that the Otago exercise program brought about positive changes to improve physical function and psychological function to help prevent disabled elderly people from falling.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.12
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• pp.5958-5967
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• 2012

The purpose of this study is to build a bioethical foundation for Paramedic students by identifying and explaining the subjective types and structures of the students' perception on abortion using Q-methodology. Forty statements as a Q sample are selected from the literature and in-depth interview, and P-sample consists of twenty nine junior Paramedic students. The collected data is analyzed by using a QUANL program. The analysis identified three types of the paramedic students' perception on abortion: The fetus-oriented decision type, The mother-oriented active decision type(n=15), The Type(n=9),and mother-oriented passive decision type(n=5). The three factors all had eigenvalues greater than 1.0 and explained 61.3% of the variance in responses. Based on the result of this research, type 1 focuses on the importance of life, whereas other types seem to demonstrate a self-centered mindse that puts more importance on self than the fetus. Therefore, bioethics courses, especially ones that deal with abortion, are crucial to those paramedic students who handle the lives of others.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.11
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• pp.1638-1652
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• 2000

Prolific females require better nutrition and feeding practice because of larger litter size and the substantial decline in body fat. Life-time pig output will be compromised if body protein and fat are not properly managed. First litter females are especially vulnerable because they can loose ${\geq}15%$ of whole-body protein. Conservation of body protein mass during first lactation minimizes wean to estrus interval and increases second litter size (up to 1.2 pigs). The ability to influence litter-size by amino acid nutrition is a new dimension in our understanding. A P2 fat depth below 12 mm at farrow and below 10 mm at wean compromised wean to estrus interval (>2 d) and next litter size (0.5 to 1.5 pigs) in sows. It is now clear that a 'modest' excess of feed during the first 72 h of pregnancy decreases embryo viability so that the potential for an increased litter size at birth is not realized. The capacity for milk production by prolific young sows is 25% higher than the standard used previously (NRC, 1988). First litter females averaged 9.82 kg milk/d for a 21 d lactation. Second and third litter counterparts averaged 10.35 kg/d. Milk production was 95% of peak by 10 d of lactation and sows were in greatest negative energy and lysine balance during the first 6 d. Nearly 45% of the total loss in body protein occurred within the first 6 d, but this could reduced to 30-35% by using a more aggressive feeding strategy after parturition. There appear to be 2 phases in lactation for lysine need (d 2-12 vs 12-21). Feeding to the higher level alleviates the second litter size decline. The lysine requirement for lactation can be predicted with accuracy, but we are not able to predict the second limiting amino acid. Mammary uptake of valine relative to lysine and recent work with practical diets suggest that the recent NRC (1998) pattern is realistic and that threonine and valine could be co-limiting for corn-soy diets for prolific sows nursing 10-11 pigs. Empirical studies are needed to refine the ideal pattern so that synthetic lysine can be used with more confidence. Milk fat output for the elite sow is extraordinary and poses an unnecessarily high energetic cost. Methods that reduce mammary fat synthesis will benefit the sow and may enhance piglet growth.

• Journal of Pharmacopuncture
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• v.18 no.4
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• pp.45-50
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• 2015

Objectives: In this study, we investigated the 4-week repeated-dose oral toxicity of gami-jakyak gamcho buja decoction (Mecasin) to develop safe treatments. Methods: In order to investigate the 4-week oral toxicity of Mecasin, we administered Mecasin orally to rats. Sprague-Dawley (SD) rats were divided into four groups of five male and five female animals per group: group 1 being the control group and groups 2, 3, and 4 being the experimental groups. Doses of Mecasin of 500, 1,000, and 2,000 mg/kg of body weight were administered to the experimental groups, and a dose of normal saline solution of 10 mL/kg was administered to the control group. We examined the survival rate, weight, clinical signs, and gross findings for four weeks. This study was conducted under the approval of the Institutional Animal Ethics Committee. Results: No deaths occurred in any of the four groups. No significant changes in weights or food consumption between the control group and the experimental groups were observed. Serum biochemistry revealed that some groups showed significant decrease in inorganic phosphorus (IP) (P < 0.05). During necropsy on the rats, one abnormal macroscopic feature, a slight loss of fur, was observed in the mid dosage (1,000 mg/kg) male group. No abnormalities were observed in any other rats. In histopathological findings, the tubular basophilia and cast of the kidney and extramedullary hematopoiesis of the spleen were found. However, those changes were minimal and had occurred naturally or sporadically. No other organ abnormalities were observed. Conclusion: During this 4-week, repeated, oral toxicity test of Mecasin in SD rats, no toxicity changes due to Mecasin were observed in any of the male or the female rats in the high dosage group. Thus, we suggest that the doses in a 13-week, repeated test should be 0, 500, 1,000, and 2,000 mg/kg respectively.

• Journal of Navigation and Port Research
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• v.42 no.4
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• pp.291-298
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• 2018

The purpose of this study was to analyze the effectiveness of on-board medical education for crew and medical managers. The satisfaction of the crew and the medical managers after undergoing the medical education was selected as a measure of effectiveness. The data collection period extended from October 2015 to March 2018 for about 2 years and 6 months, and a total of 310 questionnaires were collected and sampled. However, in this study, regression analysis was performed with only 96 samples because of the addition and modification of questionnaire items in the regression analysis. This study investigated the effect of on-board medical educational assistance (trauma education, nursing care, CPR, and overall educational content) and control variables (total boarding career, age, final education, and education year) on satisfaction. The important findings were as follows: i) Higher the degree of trauma education, higher was the satisfaction, ii) higher the degree of overall educational content, higher was the satisfaction, iii) higher the degree of CPR education, higher was the satisfaction, iv) increased total boarding career reduced the satisfaction level, v) and higher the final education, higher was the satisfaction.

• Korean Journal of Health Education and Promotion
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• v.20 no.3
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• pp.189-205
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• 2003

The purpose： This study was to get database of health service for smoking prevention through investigating the state of the students' experience, knowledge and attitude on smoking. Method： The subjects were cosisted of 545 students who were currently enrolled in 3, 4, 5 and 6th grade of 4 primary school in J community. The instruments for this study were Smoking Knowledge and Smoking Attitude questionaire (40 items) developed by WHO(l982). Frequency, percentage, t-test, ANOY A and Chi- square test with SAS program were used to analyze the data. Result： Among the students 10.8％ answered they had the experience of smoking, while 0.5％ said they are current smokers. The experience of smoking related to general characteristics were showed significantly different according to sex, grade and received or not received of smoking exhort. With regard to smokers smoking behavior, first beginning time of smoking was most by 29.8％ preschooler and the first motive of smoking was most by 80.8％ curiosity. The main smoking area is most by 31.1 ％ own house or friend's house. The main purchase route of tobacco appeared by thing which house(father or brother's tobacco) most by 34.9％. Smoking period was most less than 1 day and smoking frequency was most stops while bum sometimes. The amount for a day was less than one cigarette. Smoking time was appeared highest when curiosity occurs. Students' knowledge level about smoking prevention is high comparatively to mean 15.44(±2.66). Smoking prevention knowledge level related to general characteristics were showed significantly different according to the grade, academic score and learned or not of smoking prevention. Therefore, was expose that the smoking prevention knowledge level is high in students who results high grade, high academic score level and learned of smoking prevention. Students' attitude level about smoking prevention was high level to mean 55.90(±3.58). Smoking prevention attitude level related to general characteristics were showed significantly different according to academy score and received or not of smoking exhort(t＝2.33, p＝.020). Therefore, was expose that the smoking prevention attitude level is high in students who result high academic score level and received of smoking exhort group. Conclusion： It follows from this study that education for smoking prevention should be continued from lower grade students and teaching for refusal skill against smoking is needed.

• Korean Journal of Clinical Pharmacy
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• v.30 no.2
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• pp.113-119
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• 2020

Background: Reducing the total anticholinergic burden (AB) in older adults is recommended owing to the several peripheral and central adverse effects. This study aimed to identify the AB status of patients admitted to geriatric centers for assessing the influence of the pharmacist-involved multidisciplinary geriatric team care on reducing the AB. Methods: We retrospectively reviewed the medical records of 328 older patients hospitalized in geriatric centers from July 1, 2018 to June 30, 2019, who received comprehensive geriatric assessment and pharmaceutical interventions from a multidisciplinary geriatric team. We measured the total AB scores for the medications at the time of admission and upon hospital discharge using the Korean Anticholinergic Burden Scale (KABS). The pre-admission factors associated with high AB (KABS score ≥3) at the time of admission were identified. Results: The proportion of patients with high AB significantly decreased from 41.8% (136/328) at the time of admission to 25.0% (82/328) on discharge (p<0.001). The pre-admission AB of patients transferred from skilled nursing facilities (odds ratio[OR]: 2.85, 95% CI: 1.26-3.75), taking more than 10 medications (OR: 3.70, 95% CI: 1.55-8.82), suffering from delirium (OR: 2.80, 95% CI: 1.04-7.50), or depression (OR: 2.78, 95% CI: 1.04-7.41) were significantly high. Antipsychotics were the most frequent classes of drugs that contributed to the total KABS score at the time of admission, followed by antihistamines. Conclusions: This study demonstrated that the multidisciplinary teams for geriatric care are effective at reducing AB in older adults. The factors associated with high AB should be considered when targeting pharmaceutical care in geriatric individuals.

• Tuberculosis and Respiratory Diseases
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• v.66 no.5
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• pp.349-357
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• 2009

Background: The treatment success rates in patients with tuberculosis are known to be lower in the private sector compared to the public sector. To improve treatment outcomes in the private sector we developed a public-private collaboration model for strengthening health education and case holding activities with public health nursing in the private sector. Methods: We performed a prospective cohort study in new smear positive pulmonary tuberculosis patients treated at private hospitals, selected by non-randomization, with an intervention consisting of health education and case holding activities by specially trained public health nurses (intervention group) results were compared with cases treated without the intervention (conventional group). Physicians were asked to treat both groups routinely. The treatment outcomes of patients under treatment by the National Tuberculosis Programme were also analyzed for comparison. Results: There were 172 cases each in the intervention and conventional groups. The mean age was 48.9${\pm}$19.0 and 48.2${\pm}$19.7 in the respective groups (p=0.66). The PHN interacted with the cases in the intervention group by initial face to face interview and telephone calls an average of 7.1${\pm}$9.2 times during the initial six months. The intervention group showed a significantly higher treatment success rate, 91.6%, (Rate Ratio [RR]; 1.23, 95% Confidence Interval [CI]; 1.12-1.36), lower default, 3.6%, (RR; 0.31, 95% CI; 0.13-0.75) and transfer-out rate, 3.0%, (RR; 0.32, 95% CI; 0.12-0.86) than the conventional group where they were: 75.0%, 11.6%, 9.3%, respectively. The success rate was even higher than the rate (80.5%) of 1,027 cases treated in health centers (RR; 1.11, 95% CI; 1.05-1.17). Of the completed cases in the intervention group, 82.2% regarded the role of the public health nurse as very helpful. Conclusion: The treatment success rate, of tuberculosis patients in the private sector, was significantly improved by an intervention using a public-private collaboration model.

• Clinical and Experimental Pediatrics
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• v.58 no.6
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• pp.211-217
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• 2015

Purpose: Mycoplasma pneumoniae (MP) infection is a major cause of respiratory infection in school-aged children. Extrapulmonary manifestations of MP infection are common, but liver involvement has been rarely reported. The aim of this study was to determine the clinical characteristics of MP-associated hepatitis. Methods: This prospective study included 1,044 pediatric patients with MP infection diagnosed serologically with MP IgM at one medical center from January 2006 to December 2012. Eighty of these patients had elevated levels of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT), each greater than 50 IU/L, without any other specific liver disorder and were compared with the 964 children without liver disorders. Results: In total, 7.7% of patients with MP infection had a diagnosis of hepatitis, especially in fall and winter. The ratio of male to female patients was 1.7:1, and the mean age of the patients was 5 years and 5 months. The most common symptoms were cough, fever, and sputum. Anorexia was the most common gastrointestinal symptom, followed by nausea/vomiting, diarrhea, and abdominal pain. Mean levels of AST and ALT were 100.65 IU/L and 118.73 IU/L, respectively. Serum AST/ALT level was normalized within 7.5 days on average without complications. The mean duration of hospitalization (11.3 days) was longer for children with hepatitis than for those without hepatitis (P=0.034). Conclusion: MP-associated hepatitis is not uncommon and has a relatively good prognosis. Therefore, clinicians should be concerned about liver involvement in MP infection but avoid further unnecessary evaluation of hepatitis associated with MP.