• Title/Summary/Keyword: Number of layers

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Mospholops Mosphogicel Chauge on the Derelopmest of Duitus Oeferers of Meat Trppe Cockerds (육닭 정관의 발육에 따른 형태학적 변화)

  • 한방근;김우권;이재홍
    • Korean Journal of Animal Reproduction
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    • v.8 no.1
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    • pp.46-55
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    • 1984
  • The purpose of the experiment was to clarify morphologically normal growth pattern of the ductus deference in accordance with the sex maturity of meat-type cockerels. 1. Diameter of lumens in u, pp.r, mid and lower parts of ductus deferens, the most conspicuous enlargement of lumen was observed in the lower part. Heights of epithelial layers of ductus deferens showed abrupt growth at 12 weeks of age with subsequent gradual growth in all the part of u, pp.r, mid and lower, and heights of those at 30 weeks were a, pp.oximately 4 times as large in the u, pp.r and mid parts and 5 times as large in the lower part in contrast to those at 4 weeks of age. Thickness of muscular layer of ductus showed gradual growth in contrast with the diameter of lumen and height of epithelial layer, showing 1.3 times as large in the u, pp.r part, 1.6 times in the mid part and 1.9 times in the lower part at 30 weeks of age in contrast to the thickness at 4 weeks of age. 2. Within 10 weeks after hatching, lining cells of ductus deferens were mainly composed of round cells and columnar cells in simple columnar epithelium. During 10th to 20th week, the lining cells were mainly composed of high columnar cells and round cells in pseudostratified epithelium. From 22nd week, the lining cells were composed of pseudostratified columnar cells. Whereas round cells disa, pp.ared gradually. Enlargement of lumen and pooling of sperms in ductus deferens coincided with the maturation of seminiferous tubules. 3. In simple correlation between the values of testis weight and the values from various measurements in the ductus deferens, there was significant correlation coefficient with each other. 4. In the India ink absorption test, India ink granules were not absorbed on the epithelium of the ductus deferens, but the granules reactive to acid phosphatase a, pp.ared in a line on the free border of each parts of the ductus deferens. The granules reactive to alkaline phosphatase were noted on the luminal border of ductus deferens mainly, but weak reaction showed than acid phophatase were a, pp.ared. The granules reactive to PAS were a, pp.ared mostly near on the free border of hte epithelial cells of ductus deferens. 5. Number of sperm, Indes of sperm vitality and MRT in the different parts of ductus deferens were tended to be somewhat dominant in the mid and lower parts than in u, pp.r part, even though not significant in the statistical analysis. Ratio of sperm abnormality was tended to be relatively high in the u, pp.r part too, and in the sperm of abnormality blunted head was less in number significantly in the mid and lower part than in the u, pp.r part.

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Effects of Gamigwichulpajing-Tang on the Development of Experimentally-induced Endometriosis in Rats (가미귀출파징탕(加味歸朮破?湯)이 실험적으로 유발된 흰쥐의 자궁내막증에 미치는 영향)

  • Kim, Yeo-Yae;Lee, Dong-Nyung;Seo, Il-Bock;Kim, Hyung-Jun
    • The Journal of Korean Obstetrics and Gynecology
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    • v.19 no.2
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    • pp.162-185
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    • 2006
  • Purpose : This study was carried out to investigate the effects of Gamigwichulpajing-Tang(GGT) on the development of experimentally-induced endometriosis in rats. Materials and Methods : Endometriosis was induced in rats by auotransplanting uterine tissue to the peritoneum and devided them into three groups: (1) sham-operated group(n=8), (2) surgically induced endometriosis and untreated control group(n=8), (3)surgically induced endometriosis and GGT treated group. GGT(700mg/head) was orally administrated for 15days after operation. Then we measured the body weight, the volumes of endometriotic implants, the weight of uterus and ovary, and investigated the content of cytokines(MCP-1, $TNF-{\alpha}$, $IL-l{\beta}$) in serum and ascites. Histopathology, immunohistochemistry for COX-2, and histochemistry for mast cell in transplanted uterine tissue were performed. Results :- The $volume(mm^3)$ of endometriotic implants in GGT-treated group$(53.50\;{\pm}18.63)$ was significantly decreased(p<<0.01) compared with control group$(404.50{\pm}317.68)$. - The content(pg/ml) of MCP-1 in ascites in GGT-treated group$(4265{\pm}108)$ was significantly decreased(p<<0.001) compared with control group$(8632{\pm}1245)$. - The content(pg/ml) of $TNF-{\alpha}$ in serum in GGT-treated group$(64.5{\pm}21.6)$ was significantly decreased(p<<0.05) compared with control group$(147.1{\pm}78.2)$. - The content(pg/ml)- of $TNF-{\alpha}$ in ascites in GGT-treated group$(738.3{\pm}502.4)$ was significantly decreased(p<<0.05) compared with control group$(1245.2{\pm}362.2)$. - The percentage(%) of positive epithelial layers for COX-2 in GGT-treated group$(22.9{\pm}9.3)$ was significantly decreased(p<<0.001) compared with control group$(50.2{\pm}8.2)$. - The number of mast cells in adjacent tissue of transplanted uterine tissue in GGT-treated group$(61.4{\pm}13.9)$ was significantly decreased(p<<0.001) compared with control group$(109.3{\pm}30.2)$. - The number of mast cells in stroma of transplanted uterine tissue in GGT-treated group$(9.4{\pm}2.7)$ was significantly decreased(p<<0.001) compared with control group$(26.0{\pm}7.7)$. - Histopathologically, proliferation of endomeuiotic epithelia and stroma, and infiltration of inflammatory cells in transplanted uterine tissue of GGT-treated group were weakly observed than those of control group. Conclusion :On the basis of these results, we concluded that Gamigwichulpajing-Tang have inhibiting effects on the development of transplanted uterine tissue. And these effects may be related with decreased Production of MCP-1 and $TNF-{\alpha}$, decreased expression of COX-2, and decreased infiltration of mast cells by administration of Gamigwichulpajing-Tang.

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STUDIES ON THE TISSUE CULTURE OF PANAX GINSENG

  • Harn C
    • Proceedings of the Ginseng society Conference
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    • 1974.09a
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    • pp.9-22
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    • 1974
  • Unlike the tissue culture in animals and human being, in higher plants various parts of the plant are cultured for varied purposes, and they are named variously depending on which parts are used as explants or what purposes they are cultured for. Followings are some of the names of culture used frequently: organ culture, tissue culture, callus culture, single cell culture, meristem culture, mericlone culture, ovary culture, ovule culture, embryo culture, endosperm culture, anther culture, pollen culture, protoplast culture, etc.. As the names of the culture indicate, in some kinds of culture the explants used for culture are actually not tissues, but organs, single cells, or protoplasts. It seems, however, convenient to call all of the above-mentioned cultures grossly as tissue culture. Several kinds of tissue culture were attempted using Panax ginseng as material and some of the results were summarized below. 1. Callus culture After dormancy of the sed was broken, whole embryo or parts (hypocotyl, cotyledon and epicotyl) of partly grown embryo were cultured in the media supplemented with growth regulators. Rapid swelling occurred in a few weeks, but most of the swelling was observed only in the basal part of epicotyl, changes in the other parts of embryo appearing in much later stages. The swelling or increase in size, however, was resulted not from the divisions of cells, but from the mere expansion of cell. Real calli were formed about two months after inoculation of explants. Callus tissues developed from cortex, pith, and vascular bundle in the cases of hypo- and epicotyl, from mesophyl tissue in the case of cotyledon. Shoots developed more easily from cotyledons regardless of whether they are detached from or attached to the embryo proper. 2. Culture in the Knudson C medium When cotyledons, detached from or attached to the embryo proper, were cultured in the growth regulator-free Knudson C medium comprision only several kinds of mineral compounds and sucrose, shoot primordium or callus developed profusely and finally plantlets were produced directly from shoot primordium or indirectly through callus. In this medium epidermal cells as well as mesophyl cells of the cotyledon became meristematic and divided, changing into multinucleate cells or multicellular bodies, developing eventually into either shoot primordia or calli. 3. Anther culture Anthers were cultured in the media supplemented with various growth regulators applied singly or in combinations. Callus was formed mostly in the connective tissue of anther. Cells of anther wall layers changed in appearance, but no division occurred. Microspores of all stages in development were not changed, ruling out the possibility that microspore-originated callus might be formed. 4. Isolation of protoplast Protoplasts were isolated from young root, leaf, and epicotyl, using 0.7M D-mannitols as osmoticum and using macerozyme and cellulase respectively for maceration and digestion of the cell wall. Production in large number of naked intact protoplast was rather difficult as compared with other plant species. Fusion of protoplasts occurred infrequently mainly due to the fewer number of naked protoplasts in the solution.

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The Analysis and Design of Advanced Neurofuzzy Polynomial Networks (고급 뉴로퍼지 다항식 네트워크의 해석과 설계)

  • Park, Byeong-Jun;O, Seong-Gwon
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.39 no.3
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    • pp.18-31
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    • 2002
  • In this study, we introduce a concept of advanced neurofuzzy polynomial networks(ANFPN), a hybrid modeling architecture combining neurofuzzy networks(NFN) and polynomial neural networks(PNN). These networks are highly nonlinear rule-based models. The development of the ANFPN dwells on the technologies of Computational Intelligence(Cl), namely fuzzy sets, neural networks and genetic algorithms. NFN contributes to the formation of the premise part of the rule-based structure of the ANFPN. The consequence part of the ANFPN is designed using PNN. At the premise part of the ANFPN, NFN uses both the simplified fuzzy inference and error back-propagation learning rule. The parameters of the membership functions, learning rates and momentum coefficients are adjusted with the use of genetic optimization. As the consequence structure of ANFPN, PNN is a flexible network architecture whose structure(topology) is developed through learning. In particular, the number of layers and nodes of the PNN are not fixed in advance but is generated in a dynamic way. In this study, we introduce two kinds of ANFPN architectures, namely the basic and the modified one. Here the basic and the modified architecture depend on the number of input variables and the order of polynomial in each layer of PNN structure. Owing to the specific features of two combined architectures, it is possible to consider the nonlinear characteristics of process system and to obtain the better output performance with superb predictive ability. The availability and feasibility of the ANFPN are discussed and illustrated with the aid of two representative numerical examples. The results show that the proposed ANFPN can produce the model with higher accuracy and predictive ability than any other method presented previously.

A Study on the Calculation of Ternary Concrete Mixing using Bidirectional DNN Analysis (양방향 DNN 해석을 이용한 삼성분계 콘크리트의 배합 산정에 관한 연구)

  • Choi, Ju-Hee;Ko, Min-Sam;Lee, Han-Seung
    • Journal of the Korea Institute of Building Construction
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    • v.22 no.6
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    • pp.619-630
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    • 2022
  • The concrete mix design and compressive strength evaluation are used as basic data for the durability of sustainable structures. However, the recent diversification of mixing factors has created difficulties in calculating the correct mixing factor or setting the reference value concrete mixing design. The purpose of this study is to design a predictive model of bidirectional analysis that calculates the mixing elements of ternary concrete using deep learning, one of the artificial intelligence techniques. For the DNN-based predictive model for calculating the concrete mixing factor, performance evaluation and comparison were performed using a total of 8 models with the number of layers and the number of hidden neurons as variables. The combination calculation result was output. As a result of the model's performance evaluation, an average error rate of about 1.423% for the concrete compressive strength factor was achieved. and an average MAPE error of 8.22% for the prediction of the ternary concrete mixing factor was satisfied. Through comparing the performance evaluation for each structure of the DNN model, the DNN5L-2048 model showed the highest performance for all compounding factors. Using the learned DNN model, the prediction of the ternary concrete formulation table with the required compressive strength of 30 and 50 MPa was carried out. The verification process through the expansion of the data set for learning and a comparison between the actual concrete mix table and the DNN model output concrete mix table is necessary.

Effect of Intercropping Ratio on the Cherry Tomato with Basil on the Growth, Physiological, and Productivity Parameters on the Rooftop in Urban Agriculture (옥상 도시농업에서 방울토마토(Lycopersicon esculentum)와 바질(Ocimum basilicum)간의 공영식재가 생육, 생리, 생산성에 미치는 영향)

  • Ju, Jin-Hee;Song, Hee-Yeon;Oh, Deuk-Kyun;Park, Sun-Yeong;Yoon, Yong-Han
    • Journal of Environmental Science International
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    • v.30 no.9
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    • pp.709-717
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    • 2021
  • This study evaluated the growth, physiological responses and productivity based on the intercropping ratio of cherry tomato (Lycopersicon esculentum L.) with basil (Ocimum basilicum L.). on the rooftops to determine out the efficient ratio in urban agriculture. From April to September 2019, an experiment was conducted on the rooftop of Konkuk University Glocal Campus. Cherry tomato and basil were selected as companion plants for eco-friendly urban agriculture on the rooftops. Each plot was created with a width of 100 cm, length of 100 cm, and height of 25 cm. After installing drainage and waterproof layers from bottom to top, substrate was laid out with a height of 20 cm. Intercropping ratio was consisted of a single tomato plant (TC), 2:1 tomato to basil (T2B1), 1:1 tomato to basil (T1B1), 1:2 tomato to basil 2 (T1B2), and a single basil plant (BC), were conducted using a randomized complete plot design with five treatments and three replication (a total 15 plots). Measurements were divided into growth, physiological responses, and productivity parameters, and detailed items were investigated and analyzed by classifying them into plant height, leaf length, leaf width, number of leaves, root length, root collar caliper, chlorophyll contents, fresh weight, dry weight, number of fruit, fruit caliper, fruit weight, and sugar content. Comparative analyses of cherry tomato with basil plants by intercropping ratio, growth, physiological, and productivity responses are determined to be efficient when the ratio of cherry tomato to basil ratio is 2:1 or 1:1.

TEM ultrastmcture of the tegumental layer of Gymnophalloides seoi (Digenea: Gyinnophallidae) (참굴큰입흡충 표피층의 투과전자현미경적 미세구조)

  • Min SEO;Jong-Yil CHAI;Soon-Hyung LEE
    • Parasites, Hosts and Diseases
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    • v.33 no.3
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    • pp.165-172
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    • 1995
  • A transmission electron microscopic study was performed on the ultrastructure of the tegumental layer of GymophoLloines seoi (Digenea: Gymnophallidae) metacercarlae and adults. The metacercariae were obtained from naturally infected oysters, Crcssosoea gigas, and the adults from experimentally infected C3H mice. The tegumental layer generally revealed a small number of foldings, numerous small vacuoles, sines, and muscle bundles. Beneath the muscle layer, nuclei of the tegumental cells were located. There was little difference in the structure of the tegument between the metacercariae and adults. The oral sucker, having well-developed muscle layers, showed a similar structure to the ventral sucker except numerous foldlngs in the ventral sucker. The ventral pit was surrounded by a thin spcpiu layer, where a number of microtubules and mitochondria were seen. Around the ventral pit located well-developed circular and longitudinal muscles. The results showed that the ultrastructure of the tegumental layer of G. seoi metacercariae and adults revealed little difference from other trematodes in general. The ventral pit, a peculiar structure of this trematode, seems to function as a sphincter or an accessory adhesive organ.

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Community dynamics of Salix species during the sedimentation in Paksil-nup Wetland, Hapcheon (합천 박실늪의 퇴적에 따른 버들류 (Salix sp.)의 군집 동태)

  • Kim, Cheol-Soo;Lee, Pal-Hong;Son, Sung-Gon;Oh, Kyung-Hwan
    • Journal of Wetlands Research
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    • v.2 no.1
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    • pp.19-29
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    • 2000
  • The physico-chemical characteristics of core sediment, community dynamics of Salix species during the sedimentation were investigated in 1990~1997 for the purpose of inquiry to reveal the effects of terrestrialization on the environment and plant community in a natural wetland. The study site, Paksil-nup wetland was a valley blocked lake located near Hwang-River, Hapcheon-gun, Gyeongsangnam-do, Korea. The values of conductivity, organic matter, total nitrogen, exchangeable K, and exchangeable Ca were higher, and pH was lower in the upper layer of the core sediment. Soil properties such as available phosphorus, exchangeable Ca, exchangeable Na increased, and organic matter, total nitrogen, exchangeable K decreased during the sedimentation. Salix nipponica was the dominant species, and Salix glandulosa was subdominant among 10 Salix species. Sahx species were supposed to be the pioneer plants in shrub and tree layers during the succession in Paksil-nup wetland. Age class of SaliX species community from the epilittoral zone to the infralittoral zone were low, and age of Saljx species distributed from 2 years to 11 years. DBH, height, mean number of branches, number of herb species, and light intensity were increased, whereas density was decreased from a lower age to a higher age community. Salix nipponica was superior than Sahx purpurea var. japonica and other Salix species during the interspecific competition among Salix species.

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An Analysis of Status Quo on the Multi-layer Planting at the landscape Planting Area in Apartment and Neighborhood Parks in Seoul Metropolitan Area (조경식재공간에서 다층식재의 실태분석 -수도권 아파트와 근린공원을 중심으로-)

  • 심우경;이동익
    • Journal of the Korean Institute of Landscape Architecture
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    • v.29 no.1
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    • pp.140-151
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    • 2001
  • This study based on the theoretical understanding of multi-layer planting which have engineering, ecological and landscape benefits, was conducted to find out the status of multi-layer planting in the apartment and neighborhood park in Seoul. This study was also aimed to seek for the problematic matters, and suggest a solution on the current multi-layer planting. The results of this study were as follows; 1) Since landscape woody plants have been classified just as tree and shrub in Korea, the classification for the multi-layer planting has been unreasonable, and landscape woody plants might have been classified as tree, sub-tree and shrub, or upper, middle, and lower-layer, It could be defined that upper layer is over eight meters in full growth, middle over 3-8 meters and lower under 3 meters. 2) In apartments, the upper layer consisted of eighteen species, the middle and lower layer seven species each. In neighborhood parks, the upper layer consisted of fifteen species, and the middle and lower layer five species each. 3) In terms of planting year of the surveyed areas, there were no differences in the number of species when planting year of the apartment was divided into two groups, the first half(1900-1995) and the second(1996-2000). But, in terms of individual occupation, the percentage was decreased in upper layer, while there was increasing in middle and lower layer. 4) As the result of survey of multi-layered area, it appeared that apartment was shown 0.65 percent and neighborhood park 0.61 percent of the planted area, which was less than 1 percentage of landscape architecturally planted area. 5) In apartment, the number of individual in middle layers has been increased in the first half and the second, but with respect to the correlation with multi-layered area, the apartments had the "$\rho$=0.208", saying that increasing middle layer was scattered planting instead of multi-layered planting. 6) In planting at the apartments in Korea, the planting density was limited, because the layer division was restricted to only tree and shrub. On the contrary, it was divided into upper, middle and lower tree in Japan. Therefore, in Korea, it should be classified as the planting density by dividing into tree, sub-tree, and shrubs, or upper, middle and lower tree by the law. And, it should be considered that the multi-layered planting has a proper organic relation as well as the planting density.g density.

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Impact of the lateral mean recirculation characteristics on the near-wake and bulk quantities of the BARC configuration

  • Lunghi, Gianmarco;Pasqualetto, Elena;Rocchio, Benedetto;Mariotti, Alessandro;Salvetti, Maria Vittoria
    • Wind and Structures
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    • v.34 no.1
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    • pp.115-125
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    • 2022
  • The high-Reynolds number flow around a rectangular cylinder, having streamwise to crossflow length ratio equal to 5 is analyzed in the present paper. The flow is characterized by shear-layer separation from the upstream edges. Vortical structures of different size form from the roll-up of these shear layers, move downstream and interact with the classical vortex shedding further downstream in the wake. The corresponding mean flow is characterized by a recirculation region along the lateral surface of the cylinder, ending by mean flow reattachment close to the trailing edge. The mean flow features on the cylinder side have been shown to be highly sensitive to set-up parameters both in numerical simulations and in experiments. The results of 21 Large Eddy Simulations (LES) are analyzed herein to highlight the impact of the lateral mean recirculation characteristics on the near-wake flow features and on some bulk quantities. The considered simulations have been carried out at Reynolds number Re=DU_∞/ν=40 000, being D the crossflow dimension, U_∞ the freestream velocity and ν the kinematic viscosity of air; the flow is set to have zero angle of attack. Some simulations are carried out with sharp edges (Mariotti et al. 2017), others with different values of the rounding of the upstream edges (Rocchio et al. 2020) and an additional LES is carried out to match the value of the roundness of the upstream edges in the experiments in Pasqualetto et al. (2022). The dimensions of the mean recirculation zone vary considerably in these simulations, allowing us to single out meaningful trends. The streamwise length of the lateral mean recirculation and the streamwise distance from the upstream edge of its center are the parameters controlling the considered quantities. The wake width increases linearly with these parameters, while the vortex-shedding non-dimensional frequency shows a linear decrease. The drag coefficient also linearly decreases with increasing the recirculation length and this is due to a reduction of the suctions on the base. However, the overall variation of C_D is small. Finally, a significant, and once again linear, increase of the fluctuations of the lift coefficient is found for increasing the mean recirculation streamwise length.