• Applied Microscopy
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• v.19 no.2
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• pp.85-98
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• 1989

The ventricular myocardia of 14, 16, 18 and 20-day-old rat fetuses and newborns have been studies by light and electron microscopic morphometrics. The volume density of the myocyte and interstitial compartments as well as volume, surface and numerical density of nuclei were estimated by light microscopic morphometrics. Whereas, the volume density of myofibrils and glycogen granules as well as the volume, surface and numerical density of mitochondria were assessed by electron microscopic morphometrics. The volume density of myocyte compartment of the ventricular myocardia in developing fetuses decreased, but increased in newborn rats. On the other hand, the volume density of the interstitial compartment increased in growing fetuses and decreased in newborns. In all groups the volume, surface and numerical density of nuclei decreased gradually with elongation of myocytes. Conversely, the volume, surface and numerical density of mitochondria and volume density of myofibrils and glycogen granules in ventricular myocytes incresed. The increase in numerical density of mitochondria probably reflects an increase in metabolic activity. Sarcomere length also increased during development.

• The KIPS Transactions:PartB
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• v.16B no.2
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• pp.131-140
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• 2009

This paper presents a new algorithm to the segmentation of the FISH images. First, for segmentation of the cell nuclei from background, a threshold is estimated by using the gaussian mixture model and maximizing the likelihood function of gray value of cell images. After nuclei segmentation, overlapped nuclei and isolated nuclei need to be classified for exact nuclei analysis. For nuclei classification, this paper extracted the morphological features of the nuclei such as compactness, smoothness and moments from training data. Three probability density functions are generated from these features and they are applied to the proposed Bayesian networks as evidences. After nuclei classification, segmenting of overlapped nuclei into isolated nuclei is necessary. This paper first performs intensity gradient transform and watershed algorithm to segment overlapped nuclei. Then proposed stepwise merging strategy is applied to merge several fragments in major nucleus. The experimental results using FISH images show that our system can indeed improve segmentation performance compared to previous researches, since we performed nuclei classification before separating overlapped nuclei.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.15-15
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• 2001

We recently demonstrated that satellite regions exhibit an aberrant DNA methylation in cloned bovine embryos. Here, we examined, using bisulfite -sequencing technology, whether the inefficient demethylation of cloned donor genomes could be rescued by the presence of oocytic nuclei. Both AciI digestion and sequencing analyses showed that satellite sequence was demethylated more efficiently in cloned tetraploid blastocysts than in diploid clones. When methyl -CpG density (the number of methyl-CpG sites per string) was scored, a significant decrease was observed In tetraploids (P<0.001). These results suggest that unknown mechanisms provided by oocytic nuclei could assist the demethylation of satellite sequences in tetraploid clones.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.6 no.3
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• pp.360-367
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• 1996

The OISF (Oxidation Induced Stacking Fault)is expected to affect the electrical properties in Si single crystals, and the nuclei of OISF are believed to be formed during the crystal growing process. Initial oxygen concentration, dopant type and its density, and cooling rate are regareded as major factors on OISF formation. In this study, the variations of OISF density under various cooling rate were investigated. Si single crystal was heated to $1400^{\circ}C$ in Ar ambient and cooled down to room temperature at different cooling rate, using horizontal tube furnace. After that, they were oxidized at $1150^{\circ}C$, and then, OISF was observed with optical microscope. The relation between oxide procipitates and OISF nucleation was investigated by FTIR analysis. As a result, it was found that there exists the intermediate cooling rate range in which OISF nucleation is highly enhanced. And also, it was found that OISF nucleation is closely related with silicon oxide procipitation in Si single crystals.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
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• v.17 no.1
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• pp.27-49
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• 1987

Using ³H-proline as a radioactive tracer, the relationship between the ultrastructural differentiation and the site of protein synthesis has been investigated in developing red blood corpuscles. The general ultra-structure of erythropoietic cells in differentiation after 60 minutes of in vitro labeling has confirmed the results from previous investigations by Bessis, M., Thiery, J. and others. In dividing nuclei more than two-thirds of the labeling were present at the interface between heterochromatin and euchromatin. In less differentiated cells most of the grains in interphase cells was localized over the nucleus. As the cells continued to develope beyond a stage where cytoplasmic density was clearly increased over other cell lines in bone marrow, the majority of grains localized over the cytoplasmic area was decreased in more mature cells, as judged by the density of cytoplasm, and the structural changes in mitochondria, Golgi complex and polysomal configurations. These results show; 1) that the cytoplasm of erythroblast series does not change under in vitro conditions employed in the study; 2) that protein synthesis in the nucleus occurs largely at the interface between euchromatin and heterochromatin in active nuclei; and 3) that cytoplasmic synthesis of proteins continues to take place well into the normoblast stage solong as the physically visible polysomes are present in maturing red blood corpuscles.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.2
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• pp.48.1-48.1
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• 2015

Active galactic nuclei (AGNs) are powered by a supermassive black hole with an accretion disk and exhibit prominent broad and narrow emission lines. The unification model AGNs requires the presence of a geometrically and optically thick torus component that hides the broad line region from observers lying in the equatorial direction. The strong far UV radiation characterizing AGN spectra is expected to be scattered inelastically in the torus region to reappear around hydrogen Balmer lines or Paschen lines in the form of broad wings. Adopting a Monte Carlo technique we produce broad wings around $H{\alpha}$, $H{\beta}$ and $Pa{\alpha}$ that are formed through Raman scattering. The widths of the wings are mainly affected by the neutral column density of the torus, and the overall strengths are primarily determined by the covering factor and the column density of the neutral region. It is concluded that deep spectroscopy of AGNs of broad wings around hydrogen emission lines may shed much light on the AGN unification model.

• Journal of The Korean Astronomical Society
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• v.52 no.2
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• pp.31-40
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• 2019

A long standing problem in the study of Active Galactic Nuclei (AGNs) is that the observed VLBI core is in fact a blending of the actual AGN core (classically defined by the ${\tau}=1$ surface) and the upstream regions of the jet or optically thin flows. This blending may cause some biases in the observables of the core, such as its flux density, size or brightness temperature, which may lead to misleading interpretation of the derived quantities and physics. We study the effects of such blending under the view of the Korean VLBI Network (KVN) for a sample of AGNs at 43 GHz by comparing their observed properties with observations obtained using the Very Large Baseline Array (VLBA). Our results suggest that the observed core sizes are a factor ~ 11 larger than these of VLBA, which is similar to the factor expected by considering the different resolutions of the two facilities. We suggest the use of this factor to consider blending effects in KVN measurements. Other parameters, such as flux density or brightness temperature, seem to possess a more complicated dependence.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.2
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• pp.78.2-78.2
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• 2012

We have studied how the intergalactic magnetic field (IGMF) affects the propagation of super-GZK iron nuclei that originate from extragalactic sources within the local GZK sphere. Toward this end, we set up hypothetical sources of ultra-high-energy cosmic-rays (UHECRs), virtual observers, and the magnetized cosmic web in a model universe constructed from cosmological structure formation simulations. We then arranged a set of reference objects at high density region to represent astronomical objects formed in the large scale structure (LSS). With our model IGMF, the paths of UHE iron nuclei are deflected on average by about 70 degrees, which might indicate a nearly isotropic distribution of arrival directions. However, the separation angle between the arrival directions and the nearest reference object on the LSS is only ~6 degrees, which is twice the mean distance to the nearest neighbors among the reference objects. This means that the positional correlation of observed UHE iron events with their true sources would be erased by the IGMF, but the correlation with the LSS itself is to be sustained. We discuss implications of our findings for correlations studies of real UHECR events.