• 대한물리치료과학회지
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• 제10권1호
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• pp.198-205
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• 2003

We evaluated the effect of low power GaAsAl laser on re-epithelization in full-thickness excisional wound of rat skin. Two full-thickness excisions were made on the back of the experimental animals. Low power laser applications with 10mW intensity were treated experimental animals twice a day for 7 days. On the seventh postoperative day the quantitative analysis of re-epithelization was performed using immunohistochemical staining for proliferating cell nuclear antigen (PCNA). The majority of PCNA immunoreactive cells was observed at epithelial cells in the margin of full thickness excisional wound. The low power laser treatments significantly increased the number of PCNA immunoreactive cell as compared to that of non treated animal group (p<0.01). The shape of PCNA immunoreactive cell appeared as small dark, round to ovoid structures. Most PCNA immunoreactive cells exhibited a high intensity of staining that contrasted sharply with the surrounding background. In conclusion, these findings suggest that GaAlAs laser treatments effectively enhance the epithelial wound healing by the stimulating cell proliferation. Furthermore, the majority of cell proliferation occurred in the margin of full thickness excisional wound.

• Nuclear Engineering and Technology
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• 제38권4호
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• pp.359-374
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• 2006

Since 1991, Korea, Canada and United States have performed the direct use of spent pressurized water reactor (PWR) fuel in the Canada deuterium uranium (CANDU) reactors (DUPIC) fuel development project. Unlike the Tandem fuel cycle, which requires a wet reprocessing, the DUPIC fuel technology can directly refabricate CANDU fuels from the PWR spent fuel and, therefore, is recognized as a highly proliferation-resistant fuel cycle technology, which can be adopted even in non-proliferation treaty countries. The Korea Atomic Energy Research Institute (KAERI) has fabricated DUPIC fuel elements in a laboratory-scale remote fuel fabrication facility. KAERI has demonstrated the fuel performance in the research reactor, and has confirmed the operational feasibility and safety of a CANDU reactor loaded with the DUPIC fuel using conventional design and analysis tools, which will be the foundation of the future practical and commercial uses of DUPIC fuel.

• 방사성폐기물학회지
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• 제8권4호
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• pp.269-277
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• 2010

원자력의 중흥기를 맞이하여 민감한 핵연료주기 기술의 무분별한 확장을 억제하고자 다양한 핵연료주기 다자 방안이 제시되고 있다. 현재 원자력 공급국 위주의 핵연료주기 다자화가 추진되고 있는 실정에서 후행 핵연료주기 기술의 다자화 추진 추이를 파악하고자 사용후핵연료 공동 관리 시설에 대한 분석 결과를 검토하였다. 또한 후행 핵연료주기 연구개발 시설의 다자화를 제안하고 기대효과와 문제점을 검토한 후 이를 실현하기 위한 추진방안을 도출하였다.

• Nuclear Engineering and Technology
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• 제39권3호
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• pp.171-186
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• 2007

Critical issues in the development targets for the future fast reactor(FR) cycle system, including sodium-cooled FR were to ensure safety assurance, efficient utilization of resources, reduction of environmental burden, assurance of nuclear non-proliferation, and economic competitiveness. A promising design concept of sodium-cooled fast reactor JSFR is proposed aiming at fully satisfaction of the development targets for the next generation nuclear energy system. A roadmap toward JSFR commercialization is described, to be followed up in a new framework of the Fast reactor Cycle Technology development(FaCT) Project launched in 2006.

• Journal of Radiation Protection and Research
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• 제20권3호
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• pp.155-162
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• 1995

비전리방사선에 대한 방호의 관점에서 현재의 검출 및 측정기술을 검토하였다. 특히 전파와 자외선을 중심으로 노출계측량, 측정기기 및 측정 시 고려사항을 설명하였다. 전파장에 대해서는 실제 파원별로 출력밀도의 수준을 요약하여 실무에 참고가 되게 하였다. 대체로 비 전리방사선의 측정에는 아직 큰 오차가 있으므로 늘어나는 비 전리방사선원과 이로 인한 유해한 건강영향에 대한 대중의 관심을 고려하면 비 전리방사선 측정기술의 개발수요는 크다.

• Journal of Radiation Protection and Research
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• 제37권2호
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• pp.56-62
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• 2012

To determine the biological effects of low-dose-rate radiation ($^{137}Cs$, 2.95 mGy/h) on EL4 lymphoma cells during 24 h, we investigated the expression of genes related to apoptosis, cell cycle arrest, DNA repair, iron transport, and ribonucleotide reductase. EL4 cells were continuously exposed to low-dose-rate radiation (total dose: 70.8 mGy) for 24 h. We analyzed cell proliferation and apoptosis by trypan blue exclusion and flow cytometry, gene expression by real-time PCR, and protein levels with the apoptosis ELISA kit. Apoptosis increased in the Low-dose-rate irradiated cells, but cell number did not differ between non- (Non-IR) and Low-dose-rate irradiated (LDR-IR) cells. In concordance with apoptotic rate, the transcriptional activity of ATM, p53, p21, and Parp was upregulated in the LDR-IR cells. Similarly, Phospho-p53 (Ser15), cleaved caspase 3 (Asp175), and cleaved Parp (Asp214) expression was upregulated in the LDR-IR cells. No difference was observed in the mRNA expression of DNA repair-related genes (Msh2, Msh3, Wrn, Lig4, Neil3, ERCC8, and ERCC6) between Non-IR and LDR-IR cells. Interestingly, the mRNA of Trfc was upregulated in the LDR-IR cells. Therefore, we suggest that short-term Low-dose-rate radiation activates apoptosis in EL4 lymphoma cells.

• 한국방사성폐기물학회:학술대회논문집
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• 한국방사성폐기물학회 2003년도 가을 학술논문집
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• pp.172-179
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• 2003

핵확산 저항성 핵연료주기공정, 트리튬을 활용하는 첨단산업 시설과 중수로형 원전 등의 국내 원자력 산업에서 다양한 화합물 형태로 발생하는 트리튬 폐기물을 처리하는 기술을 개발하기 위하여 국내외 트리튬 처리기술의 현황을 조사하였음. 트리튬 폐기물을 크게 기체상, 유기상, 수용액상으로 분류하였고, 이들을 각각 처리할 수 있는 기술을 분석하였음. 이러한 트리튬 폐기물의 처리기술 개발은 원자력 산업의 지속적인 발전을 위한 기술력 기반조성 및 국민의 방사성 폐기물에 대한 긍정적 이미지를 심어 주는데 필수적임.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2020년도 춘계학술발표대회
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• pp.188-190
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• 2020

최근 4차 산업혁명과 더불어 센서 네트워크와 같은 최신 무선통신 기술들의 기반시설 적용을 위한 연구들이 활발하게 이루어지고 있다. 원자력시설 또한, 보안 및 비상대응 시스템에 무선통신을 적용하기 위한 연구들이 진행되고 있으며, 미국과 UAE의 경우 이미 원자력시설에 무선통신을 적용하여 사용하고 있다. 그러나 무선통신의 경우, 물리적인 네트워크 접근 경로가 존재하지 않기 때문에 통신 경로에 대한 접근통제가 불가능하며 광범위한 지역에 네트워크를 설치하는 경우 중계 단말 수량의 증가로 인한 접근통제 취약점이 발생할 가능성이 있다. 이와 같은 무선통신의 특성 때문에 원자력시설의 필수디지털자산에 무선 네트워크를 적용 시 현재의 통신 경로 접근통제 등의 유선 통신을 기준으로 작성된 접근통제 규제기준으로는 무선통신에 대한 접근통제를 이행하기에는 부족함이 있다. 이에 본 논문에서는 무선 네트워크 접근통제를 위한 규제 기준 개선안을 제시한다.

• Journal of Applied Biological Chemistry
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• 제57권2호
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• pp.113-122
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• 2014

A5E is complex of several medicinal herb ethanol extracts. The aim of this study is investigating the anticancer effect for non-small cell lung cancer. The antitumor effects of A5E on NCI-H460 were examined by regulation of cell proliferation, apoptosis, cell cycle arrest, mitochondrial membrane potential (${\Delta}{\Psi}_m$), and apoptosis-related protein. Cell proliferation was measured by MTS assay. Apoptosis induced by A5E was confirmed by Annexin V-fluorescein isothiocyanate (FITC)/Propidium Iodide (PI) staining, and cell cycle arrest was measured by PI staining. NF-${\kappa}B$ translocation was detected by immunofluorescence and MMP (${\Delta}{\Psi}_m$) was measured by JC-1 staining. The expression of extrinsic pathway molecules such as FasL and FADD were elevated, and procaspase-8 was processed by A5E. In addition, intrinsic pathway related molecules were altered. The Bcl-2 and Bcl-xl levels decreased, Bax increased, and cytochrome C was released. In addition, the mitochondrial membrane potential collapsed, and caspase-3 and poly-(ADP-ribose) polymerase were processed by A5E. Moreover, A5E affected the cellular survival pathway involving phosphatidylinositol 3-kinase (PI3K)/Akt and NF-${\kappa}B$. PI3K and Akt were downregulated, also NF-${\kappa}B$ expression was decreased, and nuclear translocalization was inhibited by A5E. These results suggested that A5E delays proliferation, inhibit cell cycle progression and induce apoptosis in human lung cancer cell. We conclude that A5E is a potential anticancer agent for human lung carcinoma.

• 대한한의학회지
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• 제27권3호
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• pp.51-62
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• 2006

Objective: Although chronic non-bacterial prostatitis is a common disease, it is very difficult to treat effectively. Lygodium japonicum has traditionally been used in treatment of urinary tract inflammation and voiding disturbance. In this study, we investigated the therapeutic effects and action mechanism of Lygodium japonicum in the rat model of non-bacterial prostatitis induced by castration and testosterone treatment. Methods: Five-month-old rats were treated with $17\beta-estradiol$ after castration for induction of experimental non-bacterial prostatitis, which is similar to human chronic prostatitis in histopathological profiles. Lygodium japonicum and testosterone were administered as an experimental specimen and a positive control, respectively. The prostates were evaluated by histopathological parameters including the epithelial score and epithelio-stromal ratio for glandular damage, proliferating cell nuclear antigen (PCNA) labeling index for cyto-proliferation and a TUNEL (deoxyuridine triphosphate biotin nick end-labeling) assay for cell apoptosis. Results: While prostates of control rats revealed severe acinar gland atrophy and stromal proliferation, the rats treated with Lygodium japonicum showed a lesser range of tissue damage. Epithelial score was improved in Lygodium japonicum than that of the control (P<0.05). The epithelio-stromal ratio was lower in Lygodium japonicum when compared to that of the control (P<0.05). Although there was no difference in PCNA and TUNEL positive cells of the glandular epithelia, we found an decreased number of PCNA positive cell and concurrent increase of TUNEL positive cells in the stroma of Lygodium japonicum treated rats (P<0.01). Conclusions: These findings suggest that Lygodium japonicum may protect the glandular epithelial cells and also inhibit stromal proliferation in association with suppression of cyto-proliferation and stimulation of apoptosis. We concluded that Lygodium japonicum may be a useful remedy agent for treating the chronic non-bacterial prostatitis.