• 제목/요약/키워드: Nuclear localization

검색결과 308건 처리시간 0.023초

복부농양진단을 위한 Indium-111 표지백혈구스캔 (Scintigraphy with Indium-111-oxine Labeled Leukocytes for Localization of Abscesses)

  • 김병태;이동수;이경수;최형식;김명준;양승오;이재훈;최창순;김택규;정준기;이명철;고창순
    • 대한핵의학회지
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    • 제24권1호
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    • pp.37-48
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    • 1990
  • Detection of deep-seated abscesses is sometimes difficult with ultrasonogrpahy or computed tome graphy alone. Indium-111-labeled leukocyte has widely used in the localization of abscesses after introduction by Segal and Thakur in 1976. But there are some difficulties in using indium-111-oxine in our country because of hardness to get the radiopharmaceutical timely and long time for labeling leukocytes. So we peformed the indium-111-labeled leukocyte scan for establishment of the labeling procedure and clinical application. We labeled the mixed leukocytes from 36 ml of patient's blood using 4 ml of ACD solution, 7 ml of 6% hydroxyethyl starch solution $(HESPAN^{(R)})$, 1 mCi of indium-111 oxine, 5 ml of normal saline and centrifuge. It took about 2 hours for the preparation of radiolabeled leukocytes and attention for contamination was needed. The average injected dose of labeled mixed leukocytes was 465 uCi. The average number of injected leukocytes was $2.5\times10^8$ and the labeling ratio was $57{\pm}13%$ (Table 2, Fig. 5). These number and ratio were sufficient for the localization of abscess. About twenty per cent of indium was labeled to red blood cells and platelets (Fig. 6) and the half-life of injected radiolabeled leukocytes was 8.3 hours. Scan was performed in 9 patients who were suspected to have abscesses clinically or radiologically. Three patients were positive, in one patient who had abscess close to lower lumbar vertebrae was surgically drained and another 2 positive cases did not show abscess clearly on computed tomography, so only antibiotics were administrated and treated successfully. The negative 6 patients were improved without specific treatment. In conclusion, the use of indium-111 oxine labeled leukocytes for localization of abscesses were very specific and helpful in the decision of treatment considering its relatively simple labeling method, and could be easily performed providing timely supply of the radiopharmaceutical.

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생물정보학을 이용한 연체동물의 NLS (Nuclear Localization Signals) 포함 단백질의 분석 (Bioinformatic Analysis of NLS (Nuclear Localization Signals)-containing Proteins from Mollusks)

  • 이용석;강세원;조용훈;곽희철;채성화;최상행;안인영;박홍석;한연수;고원규
    • 한국패류학회지
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    • 제22권2호
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    • pp.109-113
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    • 2006
  • 연체동물 유래 아미노산 서열 22,138 개에서 NLS가 예측되는 아미노산 서열은 266 개였으며 이는 연체동물 전체 아미노산 중 1.2% 정도였다. 또한 현재 등재되어 있는 연체동물 8,314 종 중 NLS를 포함한 아미노산이 밝혀진 생물은 60여종에 불과 하였다. 현재 알려진 연체동물 서열 중에는 두족 강의 경우가 NLS를 포함한 아미노산이 많을 것으로 예측되었다.

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Byr4p, a Possible Regulator of Mitosis and Cytokinesis in Fission Yeast, Localizes to the Spindle Pole Body by its C-Terminal Domains

  • Jwa, Mi-Ri;Shin, Se-Jeong;Albright, Charles F.;Song, Ki-Won
    • BMB Reports
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    • 제32권1호
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    • pp.92-97
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    • 1999
  • Cytokinesis and septation should be coordinated to nuclear division in the cell division cycle for precise transmission of the genome into daughter cells. byr4, an essential gene in fission yeast Schizosaccharomyces pombe, regulates the timing of cytokinesis and septation in a dosage-dependent manner. We examined the intracellular localization of the Byr4 protein by expressing byr4 as a fusion of green fluorescence protein (GFP). The Byr4 protein localizes as a single dot on the nuclear periphery of interphase cells, duplicates before mitosis, and the duplicated dots segregate with the nuclei in anaphase. The behavior of Byr4p throughout the cell cycle strongly suggests that Byr4p is localized to the spindle pole body (SPB), a microtubule organizing center (MTOC) in yeast. The presence of the Byr4 protein in the SPB is consistent with its function to coordinate mitosis and cytokinesis. We also mapped the domains of Byr4p for its proper localization to SPB by expressing various byr4 deletion mutants as GFP fusions. Analyses of the diverse byr4 deletion mutants suggest that the indirect repeats and the regions homologous to the open reading frame (ORF) YJR053W of S. cerevisiae in its C-terminus are essential for its localization to the SPB.

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발전소 제어 시스템 유지보수 방향 (Maintenance and repair of power plant control system)

  • 이종희;하달규
    • 제어로봇시스템학회:학술대회논문집
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    • 제어로봇시스템학회 1987년도 한국자동제어학술회의논문집; 한국과학기술대학, 충남; 16-17 Oct. 1987
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    • pp.625-630
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    • 1987
  • Technology associated with power plant control system has been heavily rely on foreign technology. The main reason is that the system has tomaintain stringent reliability and stability. Localization of this system can be started from localization of modules necessary for maintenance and repair in hydraulic and thermal power plant. Gradually and eventually system engineering capability can be built up to design and develop nuclear power plant control system through technology accumulation. Methods are presented to achieve this goal.

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Subcellular Localization of Diacylglycerol-responsive Protein Kinase C Isoforms in HeLa Cells

  • Kazi, Julhash U.;Kim, Cho-Rong;Soh, Jae-Won
    • Bulletin of the Korean Chemical Society
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    • 제30권9호
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    • pp.1981-1984
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    • 2009
  • Subcellular localization of protein kinase often plays an important role in determining its activity and specificity. Protein kinase C (PKC), a family of multi-gene protein kinases has long been known to be translocated to the particular cellular compartments in response to DAG or its analog phorbol esters. We used C-terminal green fluorescent protein (GFP) fusion proteins of PKC isoforms to visualize the subcellular distribution of individual PKC isoforms. Intracellular localization of PKC-GFP proteins was monitored by fluorescence microscopy after transient transfection of PKC-GFP expression vectors in the HeLa cells. In unstimulated HeLa cells, all PKC isoforms were found to be distributed throughout the cytoplasm with a few exceptions. PKC$\theta$ was mostly localized to the Golgi, and PKC$\gamma$, PKC$\delta$ and PKC$\eta$ showed cytoplasmic distribution with Golgi localization. DAG analog TPA induced translocation of PKC-GFP to the plasma membrane. PKC$\alpha$, PKC$\eta$ and PKC$\theta$ were also localized to the Golgi in response to TPA. Only PKC$\delta$ was found to be associated with the nuclear membrane after transient TPA treatment. These results suggest that specific PKC isoforms are translocated to different intracellular sites and exhibit distinct biological effects.

인간 hnRNP A1 단백질에 포함된 RGG 상자의 기능 분석 (Analysis of the Role of RGG box of human hnRNP A1 protein)

  • 최미영
    • 한국산학기술학회논문지
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    • 제18권12호
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    • pp.575-580
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    • 2017
  • 본 연구는 hnRNP A1 단백질에 포함되어 있는 RGG 상자가 이 단백질의 세포내 위치에 미치는 영향 및 이 단백질의 안정화에 미치는 영향을 분석하는 것을 목적으로 하며 2014년 10월부터 약 3년 동안 수행되었다. 이를 위해 먼저, RGG상자 내의 6개의 아르기닌을 라이신으로 돌연변이를 만든 다음 pcDNA1-HA-hnRNP A1(6R/K)를 재조합하였다. 다음, 이 플라스미드 DNA를 HeLa 세포에 형질주입하여 HA-hnRNP A1(6R/K) 단백질의 세포내 위치에 미치는 영향을 면역형광현미경법으로 분석하였다. 그 결과 hnRNP A1(6R/K) 단백질은 (wt 단백질처럼 핵에 위치하지 못하고) 핵과 세포질 모두에 퍼져 있는 것을 확인하였다. hnRNP A1(6R/K)의 안정성을 조사하기 위해서는 pcDNA1-HA-hnRNP A1(6R/K)를 HeLa 세포에 형질 주입시킨 다음 발현된 단백질을 웨스턴 블랏 실험으로 분석하였는데, 그 결과 HA-hnRNP A1(6R/K)는 (잘려서) 크기가 작아진 것을 확인할 수 있었다. 이 결과들로부터 HA-hnRNP A1(6R/K)가 핵과 세포질 모두에 퍼져 있는 것은 6R/K 돌연변이가 hnRNP A1의 핵 위치 능력에 영향을 미쳤기 때문이 아니라 6R/K 돌연변이가 일어난 부위 또는 그 부근이 절단되어 hnRNP A1의 핵 위치 신호(nuclear localization singal)인 M9 도메인이 들어있는 C-말단 부위를 잃었기 때문이라는 점을 확인 할 수 있었다. 본 연구의 결과들은 hnRNP A1에 있는 RGG 상자의 아르기닌이 hnRNP A1의 안정성에 중요한 역할을 한다는 것을 제시한다. 세균에서 발현시켜서 정제된 hnRNP A1(6R/K)의 RNA-결합 능력에 대한 영향 분석은 추후 과제로 남겨둔다.

201 Thallium Scintiscan으로 확인된 이소성 부갑상선 선종의 1예 (A Case of Ectopic Parathyroid Adenoma Detected by 201 Thallium Scintiscan)

  • 송영기;이명철;조보연;고창순;민헌기;오승근
    • 대한핵의학회지
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    • 제21권1호
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    • pp.75-78
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    • 1987
  • A 57 year old female patient was diagnosed as primary hyperparathyroidism after incidental finding of hypercalcemia. She was treated with radioiodine for Graves disease twenty years ago. Preoperative localization procedures with sonography and CT were unrevealing but 201 thallium scintiscan clearly demonstrated ectopic parathyroid adenoma which was confirmed by surgery. Brief review of the preoperative parathyroid localization procedure was done and the relationship between radioiodine therapy and hyperparathyroidism was discussed.

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신피질성 간질에서 발작기 $^{99m}Tc$-HMPAO 뇌혈류 SPECT의 간질병소 국소화 성능 (The Performance of Ictal Brain SPECT Localizing for Epileptogenic Zone in Neocortical Epilepsy)

  • 김은실;이동수;현인영;정준기;이명철;고창순;이상건;장기현
    • 대한핵의학회지
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    • 제29권4호
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    • pp.445-450
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    • 1995
  • 신피질성 간질에서 발작기 뇌혈류 SPECT는 발작기 표면뇌파도를 기준으로 국소화했을때 67%의 국소화성능을 보였다. MR에 병변을 찾지 못한 57%(12예)에서도 혈류증가부위를 9예에서 찾을 수 있었다. 그리드삽입을 위한 수술부위 결정에 도움을 받은 경우가 MR에 나타난 병변유무와 상관없었다. 반면 MR에서는 43%에서 병변을 찾았다. 일부예에서 혈류증가부위의 확산을 관찰할 수 있었고 드물게 확산이 반대쪽 반구를 포함하는 경우가 있으나 대부분 국소화에 도움이 되는 자료로 사용할 수 있었다. 수술후 간질발작이 가라앉는 것을 확인한 결과를 준거로 성능비교 연구를 확장하여야 한다고 생각하였다.

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